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  1. Article: Pseudorabies virus uses clathrin mediated endocytosis to enter PK15 swine cell line.

    Andreu, Sabina / Agúndez, Carmen / Ripa, Inés / López-Guerrero, José Antonio / Bello-Morales, Raquel

    Frontiers in microbiology

    2024  Volume 15, Page(s) 1332175

    Abstract: Pseudorabies virus (PRV), a herpesvirus responsible for Aujeszky's disease, causes high mortality in swine populations. To develop effective and novel antiviral strategies, it is essential to understand the mechanism of entry used by PRV to infect its ... ...

    Abstract Pseudorabies virus (PRV), a herpesvirus responsible for Aujeszky's disease, causes high mortality in swine populations. To develop effective and novel antiviral strategies, it is essential to understand the mechanism of entry used by PRV to infect its host. Viruses have different ways of entering host cells. Among others, they can use endocytosis, a fundamental cellular process by which substances from the external environment are internalized into the cell. This process is classified into clathrin-mediated endocytosis (CME) and clathrin-independent endocytosis (CIE), depending on the role of clathrin. Although the involvement of cholesterol-rich lipid rafts in the entry of PRV has already been described, the importance of other endocytic pathways involving clathrin remains unexplored to date. Here, we characterize the role of CME in PRV entry into the PK15 swine cell line. By using CME inhibitory drugs, we report a decrease in PRV infection when the CME pathway is blocked. We also perform the shRNA knockdown of the μ-subunit of the adaptor protein AP-2 (AP2M1), which plays an important role in the maturation of clathrin-coated vesicles, and the infection is greatly reduced when this subunit is knocked down. Furthermore, transmission electron microscopy images report PRV virions inside clathrin-coated vesicles. Overall, this study suggests for the first time that CME is a mechanism used by PRV to enter PK15 cells and provides valuable insights into its possible routes of entry.
    Language English
    Publishing date 2024-02-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2024.1332175
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  2. Article ; Online: Liposomal Lactoferrin Exerts Antiviral Activity against HCoV-229E and SARS-CoV-2 Pseudoviruses In Vitro.

    Andreu, Sabina / Ripa, Inés / Bello-Morales, Raquel / López-Guerrero, José Antonio

    Viruses

    2023  Volume 15, Issue 4

    Abstract: A limited number of effective therapies are currently available to treat human coronavirus SARS-CoV-2 and other human coronaviruses, which are responsible for nearly a third of global cases of the common cold. The possibility of new emerging ... ...

    Abstract A limited number of effective therapies are currently available to treat human coronavirus SARS-CoV-2 and other human coronaviruses, which are responsible for nearly a third of global cases of the common cold. The possibility of new emerging coronaviruses demands powerful new antiviral strategies. Lactoferrin is a well-known protein that possesses anti-inflammatory and immunomodulatory activities, and it has previously shown antiviral activity against several viruses, including SARS-CoV-2. To increase this antiviral activity, here we present bovine liposomal lactoferrin. Liposomal encapsulation of the compound was proven to increase permeability, bioavailability, and time release. In the present work, we compare the antiviral activity of free and liposomal bovine lactoferrin against HCoV229E and SARS-CoV-2 in vitro and in human primary bronchial epithelial cells, and we demonstrated that the liposomal form exerts a more potent antiviral activity than its free form at non-cytotoxic doses.
    MeSH term(s) Humans ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; SARS-CoV-2 ; Coronavirus 229E, Human ; Lactoferrin/pharmacology ; COVID-19 ; Liposomes ; RNA Viruses
    Chemical Substances Antiviral Agents ; Lactoferrin (EC 3.4.21.-) ; Liposomes
    Language English
    Publishing date 2023-04-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15040972
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Soft Tissue Sarcomas with Chromosomal Alterations in the 12q13-15 Region: Differential Diagnosis and Therapeutic Implications.

    Lavernia, Javier / Claramunt, Reyes / Romero, Ignacio / López-Guerrero, José Antonio / Llombart-Bosch, Antonio / Machado, Isidro

    Cancers

    2024  Volume 16, Issue 2

    Abstract: The chromosomal region 12q13-15 is rich in oncogenes and contains several genes involved in the pathogenesis of various mesenchymal neoplasms. Notable genes in this region ... ...

    Abstract The chromosomal region 12q13-15 is rich in oncogenes and contains several genes involved in the pathogenesis of various mesenchymal neoplasms. Notable genes in this region include
    Language English
    Publishing date 2024-01-19
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers16020432
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  4. Article ; Online: Real-world experience with trabectedin for the treatment of recurrent ovarian cancer.

    Romero, Ignacio / López-Guerrero, José Antonio / Pignata, Sandro

    Expert review of anticancer therapy

    2021  Volume 21, Issue 10, Page(s) 1089–1095

    Abstract: ... ...

    Abstract Introduction
    MeSH term(s) Antineoplastic Combined Chemotherapy Protocols/adverse effects ; Dioxoles ; Doxorubicin ; Humans ; Neoplasm Recurrence, Local/drug therapy ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/pathology ; Polyethylene Glycols ; Prospective Studies ; Retrospective Studies ; Tetrahydroisoquinolines/adverse effects ; Trabectedin
    Chemical Substances Dioxoles ; Tetrahydroisoquinolines ; Polyethylene Glycols (3WJQ0SDW1A) ; Doxorubicin (80168379AG) ; Trabectedin (ID0YZQ2TCP)
    Language English
    Publishing date 2021-06-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2112544-2
    ISSN 1744-8328 ; 1473-7140
    ISSN (online) 1744-8328
    ISSN 1473-7140
    DOI 10.1080/14737140.2021.1941890
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5907: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: The hallmarks of ovarian cancer: proliferation and cell growth.

    López-Reig, Raquel / López-Guerrero, José Antonio

    EJC supplements : EJC : official journal of EORTC, European Organization for Research and Treatment of Cancer ... [et al.

    2020  Volume 15, Page(s) 27–37

    Abstract: Epithelial ovarian cancer (EOC) is a heterogeneous group of diseases with distinct biological and clinical behaviour. Despite the differences between them, the capability of tumour cells to continuously proliferate and avoid death is maintained among ... ...

    Abstract Epithelial ovarian cancer (EOC) is a heterogeneous group of diseases with distinct biological and clinical behaviour. Despite the differences between them, the capability of tumour cells to continuously proliferate and avoid death is maintained among histotypes. This ability is the result of alterations at different levels, causing the deregulation of cell cycle and proliferative-related pathways. Even if the leading role is played by RB and TP53, changes in other molecular pathways are involved in the development of EOC. This ability can be exploited to generate
    Language English
    Publishing date 2020-08-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2125676-7
    ISSN 1359-6349
    ISSN 1359-6349
    DOI 10.1016/j.ejcsup.2019.12.001
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 456 -Suppl.-: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 457 -Suppl.-: Show issues

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  6. Article ; Online: Interplay between Autophagy and Herpes Simplex Virus Type 1: ICP34.5, One of the Main Actors.

    Ripa, Inés / Andreu, Sabina / López-Guerrero, José Antonio / Bello-Morales, Raquel

    International journal of molecular sciences

    2022  Volume 23, Issue 21

    Abstract: Herpes simplex virus type 1 (HSV-1) is a neurotropic virus that occasionally may spread to the central nervous system (CNS), being the most common cause of sporadic encephalitis. One of the main neurovirulence factors of HSV-1 is the protein ICP34.5, ... ...

    Abstract Herpes simplex virus type 1 (HSV-1) is a neurotropic virus that occasionally may spread to the central nervous system (CNS), being the most common cause of sporadic encephalitis. One of the main neurovirulence factors of HSV-1 is the protein ICP34.5, which although it initially seems to be relevant only in neuronal infections, it can also promote viral replication in non-neuronal cells. New ICP34.5 functions have been discovered during recent years, and some of them have been questioned. This review describes the mechanisms of ICP34.5 to control cellular antiviral responses and debates its most controversial functions. One of the most discussed roles of ICP34.5 is autophagy inhibition. Although autophagy is considered a defense mechanism against viral infections, current evidence suggests that this antiviral function is only one side of the coin. Different types of autophagic pathways interact with HSV-1 impairing or enhancing the infection, and both the virus and the host cell modulate these pathways to tip the scales in its favor. In this review, we summarize the recent progress on the interplay between autophagy and HSV-1, focusing on the intricate role of ICP34.5 in the modulation of this pathway to fight the battle against cellular defenses.
    MeSH term(s) Humans ; Herpesvirus 1, Human/physiology ; Viral Proteins/metabolism ; Autophagy/physiology ; Virus Replication ; Antiviral Agents/metabolism ; Herpes Simplex/metabolism
    Chemical Substances Viral Proteins ; Antiviral Agents
    Language English
    Publishing date 2022-11-07
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232113643
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  7. Article ; Online: Extracellular Polymeric Substances: Still Promising Antivirals.

    Bello-Morales, Raquel / Andreu, Sabina / Ruiz-Carpio, Vicente / Ripa, Inés / López-Guerrero, José Antonio

    Viruses

    2022  Volume 14, Issue 6

    Abstract: Sulfated polysaccharides and other polyanions have been promising candidates in antiviral research for decades. These substances gained attention as antivirals when they demonstrated a high inhibitory effect in vitro against human immunodeficiency virus ( ...

    Abstract Sulfated polysaccharides and other polyanions have been promising candidates in antiviral research for decades. These substances gained attention as antivirals when they demonstrated a high inhibitory effect in vitro against human immunodeficiency virus (HIV) and other enveloped viruses. However, that initial interest was followed by wide skepticism when in vivo assays refuted the initial results. In this paper we review the use of sulfated polysaccharides, and other polyanions, in antiviral therapy, focusing on extracellular polymeric substances (EPSs). We maintain that, in spite of those early difficulties, the use of polyanions and, specifically, the use of EPSs, in antiviral therapy should be reconsidered. We base our claim in several points. First, early studies showed that the main disadvantage of sulfated polysaccharides and polyanions is their low bioavailability, but this difficulty can be overcome by the use of adequate administration strategies, such as nebulization of aerosols to gain access to respiratory airways. Second, several sulfated polysaccharides and EPSs have demonstrated to be non-toxic in animals. Finally, these macromolecules are non-specific and therefore they might be used against different variants or even different viruses.
    MeSH term(s) Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Extracellular Polymeric Substance Matrix ; HIV Infections/drug therapy ; Polysaccharides/pharmacology
    Chemical Substances Antiviral Agents ; Polysaccharides
    Language English
    Publishing date 2022-06-19
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061337
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells.

    Bello-Morales, Raquel / López-Guerrero, José Antonio

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 2060, Page(s) 305–317

    Abstract: Extracellular vesicles (EVs) are secreted membrane vesicles, derived from endosomes or from the plasma membrane, which have been isolated from most cell types and biological fluids. Although EVs are highly heterogeneous and their classification is ... ...

    Abstract Extracellular vesicles (EVs) are secreted membrane vesicles, derived from endosomes or from the plasma membrane, which have been isolated from most cell types and biological fluids. Although EVs are highly heterogeneous and their classification is complex, two major categories can be distinguished: microvesicles (MVs), which derive from the shedding of the plasma membrane, and exosomes, which correspond to intraluminal vesicles released to the extracellular milieu upon fusion of multivesicular bodies (MVBs) with the plasma membrane. Cells infected with viruses may secrete MVs containing viral proteins, RNAs and, in some instances, infectious virions. A recent study carried out by our laboratory has shown that MVs released by cells infected with HSV-1 contained virions and were endocytosed by naïve cells leading to a productive infection. This suggests that HSV-1 may use MVs for spreading, expanding its tropism and evading the host immune response. Here we describe in detail the methods used to isolate and analyse the MVs released from HSV-1-infected cells.
    MeSH term(s) Cell Line ; Cell-Derived Microparticles/chemistry ; Cell-Derived Microparticles/metabolism ; Cell-Derived Microparticles/virology ; Herpes Simplex/metabolism ; Herpes Simplex/pathology ; Herpesvirus 1, Human/chemistry ; Herpesvirus 1, Human/isolation & purification ; Herpesvirus 1, Human/metabolism ; Humans
    Language English
    Publishing date 2019-10-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9814-2_17
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  9. Article ; Online: ETV6::NTRK3

    Machado, Isidro / Claramunt-Alonso, Reyes / Lavernia, Javier / Romero, Ignacio / Barrios, María / Safont, María José / Santonja, Nuria / Navarro, Lara / López-Guerrero, José Antonio / Llombart-Bosch, Antonio

    International journal of molecular sciences

    2024  Volume 25, Issue 7

    Abstract: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract, with proto-oncogene, receptor tyrosine kinase ( ...

    Abstract Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract, with proto-oncogene, receptor tyrosine kinase (
    MeSH term(s) Female ; Humans ; Gastrointestinal Stromal Tumors/drug therapy ; Gastrointestinal Stromal Tumors/genetics ; Tertiary Lymphoid Structures ; In Situ Hybridization, Fluorescence ; Receptor, Platelet-Derived Growth Factor alpha/genetics ; Immunotherapy ; Proto-Oncogene Proteins c-kit ; Receptor Protein-Tyrosine Kinases
    Chemical Substances Receptor, Platelet-Derived Growth Factor alpha (EC 2.7.10.1) ; Proto-Oncogene Proteins c-kit (EC 2.7.10.1) ; Receptor Protein-Tyrosine Kinases (EC 2.7.10.1)
    Language English
    Publishing date 2024-03-26
    Publishing country Switzerland
    Document type Review ; Case Reports
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25073707
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  10. Article: Extracellular Polymeric Substances: Still Promising Antivirals

    Bello-Morales, Raquel / Andreu, Sabina / Ruiz-Carpio, Vicente / Ripa, Inés / López-Guerrero, José Antonio

    Viruses. 2022 June 19, v. 14, no. 6

    2022  

    Abstract: Sulfated polysaccharides and other polyanions have been promising candidates in antiviral research for decades. These substances gained attention as antivirals when they demonstrated a high inhibitory effect in vitro against human immunodeficiency virus ( ...

    Abstract Sulfated polysaccharides and other polyanions have been promising candidates in antiviral research for decades. These substances gained attention as antivirals when they demonstrated a high inhibitory effect in vitro against human immunodeficiency virus (HIV) and other enveloped viruses. However, that initial interest was followed by wide skepticism when in vivo assays refuted the initial results. In this paper we review the use of sulfated polysaccharides, and other polyanions, in antiviral therapy, focusing on extracellular polymeric substances (EPSs). We maintain that, in spite of those early difficulties, the use of polyanions and, specifically, the use of EPSs, in antiviral therapy should be reconsidered. We base our claim in several points. First, early studies showed that the main disadvantage of sulfated polysaccharides and polyanions is their low bioavailability, but this difficulty can be overcome by the use of adequate administration strategies, such as nebulization of aerosols to gain access to respiratory airways. Second, several sulfated polysaccharides and EPSs have demonstrated to be non-toxic in animals. Finally, these macromolecules are non-specific and therefore they might be used against different variants or even different viruses.
    Keywords Human immunodeficiency virus ; antiviral agents ; atomization ; bioavailability ; polymers ; polysaccharides ; therapeutics
    Language English
    Dates of publication 2022-0619
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061337
    Database NAL-Catalogue (AGRICOLA)

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