Article ; Online: Selection and characterization of two Bacillus thuringiensis strains showing nematicidal activity against Caenorhabditis elegans and Meloidogyne incognita.
2021 Volume 368, Issue 5
Abstract: Bacillus thuringiensis has been widely used as a biological control agent against insect pests. Additionally, nematicidal strains have been under investigation. In this report, 310 native strains of B. thuringiensis against Caenorhabditis elegans were ... ...
| Abstract | Bacillus thuringiensis has been widely used as a biological control agent against insect pests. Additionally, nematicidal strains have been under investigation. In this report, 310 native strains of B. thuringiensis against Caenorhabditis elegans were tested. Only the LBIT-596 and LBIT-107 strains showed significant mortality. LC50s of spore-crystal complexes were estimated at 37.18 and 31.89 μg/mL for LBIT-596 and LBIT-107 strains, respectively, while LC50s of partially purified crystals was estimated at 23.76 and 20.25 μg/mL for LBIT-596 and LBIT-107, respectively. The flagellin gene sequence and plasmid patterns indicated that LBIT-596 and LBIT-107 are not related to each other. Sequences from internal regions of a cry5B and a cyt1A genes were found in the LBIT-596 strain, while a cry21A, a cry14A and a cyt1A genes were found in the LBIT-107 strain. Genome sequence of the LBIT-107 strain showed new cry genes, along with other virulence factors, hence, total nematicidal activity of the LBIT-107 strain may be the result of a multifactorial effect. The highlight of this contribution is that translocation of spore-crystal suspensions of LBIT-107 into tomato plants inoculated at their rhizosphere decreased up to 90% the number of galls of Meloidogyne incognita, perhaps the most important nematode pest in the world. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Animals ; Antinematodal Agents/metabolism ; Bacillus thuringiensis/genetics ; Bacillus thuringiensis/metabolism ; Bacillus thuringiensis Toxins/genetics ; Biological Control Agents/metabolism ; Caenorhabditis elegans/microbiology ; Endotoxins/genetics ; Flagellin/genetics ; Hemolysin Proteins/genetics ; Lycopersicon esculentum/parasitology ; Plant Diseases/parasitology ; Plant Diseases/therapy ; Plasmids/genetics ; Tylenchoidea/microbiology ; Virulence Factors/genetics | |||||
| Chemical Substances | Antinematodal Agents ; Bacillus thuringiensis Toxins ; Biological Control Agents ; Endotoxins ; Hemolysin Proteins ; Virulence Factors ; insecticidal crystal protein, Bacillus Thuringiensis ; Flagellin (12777-81-0) | |||||
| Language | English | |||||
| Publishing date | 2021-03-15 | |||||
| Publishing country | England | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 752343-9 | |||||
| ISSN | 1574-6968 ; 0378-1097 | |||||
| ISSN (online) | 1574-6968 | |||||
| ISSN | 0378-1097 | |||||
| DOI | 10.1093/femsle/fnaa186 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1372: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.