LIVIVO - Search results -

Search results

Result 1 - 10 of total 64

Search options

Article ; Online: Dodging Endosomes: Effective Cytosolic Antibody Delivery.

Löwik, Dennis W P M

Chembiochem : a European journal of chemical biology

2017 Volume 18, Issue 22, Page(s) 2196–2198

Abstract: On the inside: New methodologies for delivering antibodies right into the cytosol of cells either directly across the plasma membrane or by allowing the antibody to escape from endosomes have been proposed recently by the Cardoso/Hackenberger and Futaki ... ...

Abstract	On the inside: New methodologies for delivering antibodies right into the cytosol of cells either directly across the plasma membrane or by allowing the antibody to escape from endosomes have been proposed recently by the Cardoso/Hackenberger and Futaki groups, respectively.
Language	English
Publishing date	2017-11-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2020469-3
ISSN	1439-7633 ; 1439-4227
ISSN (online)	1439-7633
ISSN	1439-4227
DOI	10.1002/cbic.201700510
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- Full text online
- Order with fees

Article: Delivery of Various Cargos into Cancer Cells and Tissues via Cell-Penetrating Peptides: A Review of the Last Decade.

Shoari, Alireza / Tooyserkani, Raheleh / Tahmasebi, Mehdi / Löwik, Dennis W P M

Pharmaceutics

2021 Volume 13, Issue 9

Abstract: Cell-penetrating peptides (CPPs), also known as protein transduction domains, are a class of diverse amino acid sequences with the ability to cross cellular membranes. CPPs can deliver several bioactive cargos, including proteins, peptides, nucleic acids ...

Abstract	Cell-penetrating peptides (CPPs), also known as protein transduction domains, are a class of diverse amino acid sequences with the ability to cross cellular membranes. CPPs can deliver several bioactive cargos, including proteins, peptides, nucleic acids and chemotherapeutics, into cells. Ever since their discovery, synthetic and natural CPPs have been utilized in therapeutics delivery, gene editing and cell imaging in fundamental research and clinical experiments. Over the years, CPPs have gained significant attention due to their low cytotoxicity and high transduction efficacy. In the last decade, multiple investigations demonstrated the potential of CPPs as carriers for the delivery of therapeutics to treat various types of cancer. Besides their remarkable efficacy owing to fast and efficient delivery, a crucial benefit of CPP-based cancer treatments is delivering anticancer agents selectively, rather than mediating toxicities toward normal tissues. To obtain a higher therapeutic index and to improve cell and tissue selectivity, CPP-cargo constructions can also be complexed with other agents such as nanocarriers and liposomes to obtain encouraging outcomes. This review summarizes various types of CPPs conjugated to anticancer cargos. Furthermore, we present a brief history of CPP utilization as delivery systems for anticancer agents in the last decade and evaluate several reports on the applications of CPPs in basic research and preclinical studies.
Language	English
Publishing date	2021-09-02
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics13091391
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Activation of cell-penetrating peptide fragments by disulfide formation.

Tooyserkani, Raheleh / Lipiński, Wojciech / Willemsen, Bob / Löwik, Dennis W P M

Amino acids

2020 Volume 52, Issue 8, Page(s) 1161–1168

Abstract: Three cell-penetrating peptides (CPPs), Tat, Pep-3 and penetratin, were split into two parts and each fragment was terminated with a cysteine residue, to allow disulfide bridge formation, as well as a fluorescent label, for visualization and quantitative ...

Abstract	Three cell-penetrating peptides (CPPs), Tat, Pep-3 and penetratin, were split into two parts and each fragment was terminated with a cysteine residue, to allow disulfide bridge formation, as well as a fluorescent label, for visualization and quantitative analysis. After disulfide formation between two complementary CPP fragments, cellular uptake of the resulting conjugates was observed. As confirmed by in vitro experiments, the conjugated peptides showed uptake activity comparable to the native CPP sequences, while the truncated peptides were hardly active. Until now, this split CPP strategy has only been demonstrated for oligo-arginine CPPs, but here we demonstrate that it is also applicable to other cell-penetrating peptides. This wider applicability may help in the design of new activatable cell-penetrating peptides for, e.g., targeted drug delivery.
MeSH term(s)	Cell-Penetrating Peptides/chemistry ; Disulfides/chemistry ; Fluoresceins/chemistry ; HeLa Cells ; Humans ; Microscopy, Fluorescence/methods ; Peptide Fragments/chemistry ; Peptides/chemistry ; tat Gene Products, Human Immunodeficiency Virus/chemistry
Chemical Substances	Cell-Penetrating Peptides ; Disulfides ; Fluoresceins ; Pep-3 peptide ; Peptide Fragments ; Peptides ; tat Gene Products, Human Immunodeficiency Virus ; tat peptide (49-57), Human immunodeficiency virus 1 ; 6-carboxyfluorescein (3301-79-9) ; penetratin (A2AQZ20TEK)
Language	English
Publishing date	2020-07-31
Publishing country	Austria
Document type	Journal Article
ZDB-ID	1121341-3
ISSN	1438-2199 ; 0939-4451
ISSN (online)	1438-2199
ISSN	0939-4451
DOI	10.1007/s00726-020-02880-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3490: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Activatable cell-penetrating peptides: 15 years of research.

de Jong, Heleen / Bonger, Kimberly M / Löwik, Dennis W P M

RSC chemical biology

2020 Volume 1, Issue 4, Page(s) 192–203

Abstract: An important hurdle for the intracellular delivery of large cargo is the cellular membrane, which protects the cell from exogenous substances. Cell-penetrating peptides (CPPs) can cross this barrier but their use as drug delivery vehicles is hampered by ... ...

Abstract	An important hurdle for the intracellular delivery of large cargo is the cellular membrane, which protects the cell from exogenous substances. Cell-penetrating peptides (CPPs) can cross this barrier but their use as drug delivery vehicles is hampered by their lack of cell type specificity. Over the past years, several approaches have been explored to control the activity of CPPs that can be primed for cellular uptake. Since the first report on such activatable CPPs (ACPPs) in 2004, various methods of activation have been developed. Here, we provide an overview of the different ACPPs strategies known to date and summarize the benefits, drawbacks, and future directions.
Language	English
Publishing date	2020-08-26
Publishing country	England
Document type	Journal Article ; Review
ISSN	2633-0679
ISSN (online)	2633-0679
DOI	10.1039/d0cb00114g
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Activation of cell-penetrating peptide fragments by disulfide formation

Tooyserkani, Raheleh / Lipiński, Wojciech / Willemsen, Bob / Löwik, Dennis W. P. M

Amino acids. 2020 Aug., v. 52, no. 8

2020

Abstract	Three cell-penetrating peptides (CPPs), Tat, Pep-3 and penetratin, were split into two parts and each fragment was terminated with a cysteine residue, to allow disulfide bridge formation, as well as a fluorescent label, for visualization and quantitative analysis. After disulfide formation between two complementary CPP fragments, cellular uptake of the resulting conjugates was observed. As confirmed by in vitro experiments, the conjugated peptides showed uptake activity comparable to the native CPP sequences, while the truncated peptides were hardly active. Until now, this split CPP strategy has only been demonstrated for oligo-arginine CPPs, but here we demonstrate that it is also applicable to other cell-penetrating peptides. This wider applicability may help in the design of new activatable cell-penetrating peptides for, e.g., targeted drug delivery.
Keywords	cysteine ; disulfide bonds ; disulfides ; drugs ; fluorescence ; peptides ; quantitative analysis
Language	English
Dates of publication	2020-08
Size	p. 1161-1168.
Publishing place	Springer Vienna
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	1121341-3
ISSN	1438-2199 ; 0939-4451
ISSN (online)	1438-2199
ISSN	0939-4451
DOI	10.1007/s00726-020-02880-x
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 3490: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Comparison of Bioorthogonally Cross-Linked Hydrogels for

Zhan, Henan / de Jong, Heleen / Löwik, Dennis W P M

ACS applied bio materials

2019 Volume 2, Issue 7, Page(s) 2862–2871

Abstract: Hydrogels are water-saturated polymer networks and extensively used in drug delivery, tissue repair engineering, and cell cultures. For encapsulation of drugs or cells, the possibility to form ... ...

Abstract	Hydrogels are water-saturated polymer networks and extensively used in drug delivery, tissue repair engineering, and cell cultures. For encapsulation of drugs or cells, the possibility to form hydrogels
Language	English
Publishing date	2019-06-28
Publishing country	United States
Document type	Journal Article
ISSN	2576-6422
ISSN (online)	2576-6422
DOI	10.1021/acsabm.9b00253
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Self-recovering dual cross-linked hydrogels based on bioorthogonal click chemistry and ionic interactions.

Zhan, Henan / Jiang, Shanshan / Jonker, Anika M / Pijpers, Imke A B / Löwik, Dennis W P M

Journal of materials chemistry. B

2020 Volume 8, Issue 27, Page(s) 5912–5920

Abstract: The biocompatible, injectable and high water-swollen nature of hydrogels makes them a popular candidate to imitate the extracellular matrix (ECM) for tissue engineering both in vitro and in vivo. However, commonly used covalently cross-linked hydrogels, ... ...

Abstract	The biocompatible, injectable and high water-swollen nature of hydrogels makes them a popular candidate to imitate the extracellular matrix (ECM) for tissue engineering both in vitro and in vivo. However, commonly used covalently cross-linked hydrogels, despite their stability and tunability, are elastic and deteriorate as bulk material degrades which would impair proper cell function. To improve these deficiencies, here, we present a self-recovering cross-linked hydrogel formed instantaneously with functionalized poly(ethylene glycol) as a basis. We combine covalent cross-links introduced via a strain-promoted azide-alkyne cycloaddition (SPAAC) click reaction and non-covalent links between phosphonate groups and calcium ions. By adjusting the ratios of non-covalent and covalent cross-links, we synthesized these dual cross-linked (DC) hydrogels that displayed storage moduli below ∼2000 Pa and relaxation times from seconds to minutes. The gels recovered to 41-96% of their initial mechanical properties after two subsequent strain failures. Cryo-scanning electron microscopy revealed that DC hydrogels containing approximately equal amounts of covalent and non-covalent cross-links displayed phase separation. Finally, we functionalized the DC hydrogels by incorporating an integrin binding motif, RGDS, to provide a biocompatible environment for human mesenchymal stem cells (HMSCs) by facilitating adhesion inside the gel network. Inside these DC gels HSMCs displayed a viability up to 73% after five days of cell culture.
MeSH term(s)	Alkynes/chemistry ; Azides/chemistry ; Biocompatible Materials/chemistry ; Biocompatible Materials/metabolism ; Calcium/chemistry ; Cations, Divalent/chemistry ; Cell Proliferation ; Cells, Cultured ; Click Chemistry ; Cross-Linking Reagents/chemistry ; Cycloaddition Reaction ; Extracellular Matrix/metabolism ; Humans ; Hydrogels/chemistry ; Hydrogels/metabolism ; Mechanical Phenomena ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Oligopeptides/chemistry ; Organophosphonates/chemistry ; Polyethylene Glycols/chemistry ; Polyethylene Glycols/metabolism ; Rheology ; Tissue Engineering ; Tissue Scaffolds/chemistry
Chemical Substances	Alkynes ; Azides ; Biocompatible Materials ; Cations, Divalent ; Cross-Linking Reagents ; Hydrogels ; Oligopeptides ; Organophosphonates ; Polyethylene Glycols (3WJQ0SDW1A) ; arginyl-glycyl-aspartyl-serine (AC6UDA2MFC) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2020-06-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/d0tb01042a
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Activity Sensing of Coagulation and Fibrinolytic Proteases.

Sondag, Daan / Verhoeven, Stijn / Löwik, Dennis W P M / van Geffen, Mark / Veer, Cornelis Van't / van Heerde, Waander L / Boltje, Thomas J / Rutjes, Floris P J T

Chemistry (Weinheim an der Bergstrasse, Germany)

2023 Volume 29, Issue 18, Page(s) e202203473

Abstract: The blood coagulation cascade is a complex physiological process involving the action of multiple coupled enzymes, cofactors, and substrates, ultimately leading to clot formation. Serine proteases have a crucial role, and aberrations in their activity ... ...

Abstract	The blood coagulation cascade is a complex physiological process involving the action of multiple coupled enzymes, cofactors, and substrates, ultimately leading to clot formation. Serine proteases have a crucial role, and aberrations in their activity can lead to life-threatening bleeding disorders and thrombosis. This review summarizes the essential proteases involved in blood coagulation and fibrinolysis, the endogenous peptide sequences they recognize and hydrolyze, and synthetic peptide probes based on these sequences to measure their activity. The information in this review can contribute to developing novel anticoagulant therapies and specific substrates for point-of-care diagnosis of coagulation pathologies.
MeSH term(s)	Humans ; Blood Coagulation ; Fibrinolysis/physiology ; Thrombosis ; Serine Proteases ; Serine Endopeptidases
Chemical Substances	Serine Proteases (EC 3.4.-) ; Serine Endopeptidases (EC 3.4.21.-)
Language	English
Publishing date	2023-02-07
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202203473
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Solid-Phase Synthesis of Caged Luminescent Peptides via Side Chain Anchoring.

Sondag, Daan / Heming, Jurriaan J A / Löwik, Dennis W P M / Krivosheeva, Elena / Lejeune, Denise / van Geffen, Mark / Van't Veer, Cornelis / van Heerde, Waander L / Beens, Marjolijn C J / Kuijpers, Brian H M / Boltje, Thomas J / Rutjes, Floris P J T

Bioconjugate chemistry

2023 Volume 34, Issue 12, Page(s) 2234–2242

Abstract: The synthesis of caged luminescent peptide substrates remains challenging, especially when libraries of the substrates are required. Most currently available synthetic methods rely on a solution-phase approach, which is less suited for parallel synthesis ...

Abstract	The synthesis of caged luminescent peptide substrates remains challenging, especially when libraries of the substrates are required. Most currently available synthetic methods rely on a solution-phase approach, which is less suited for parallel synthesis purposes. We herein present a solid-phase peptide synthesis (SPPS) method for the synthesis of caged aminoluciferin peptides via side chain anchoring of the P
MeSH term(s)	Solid-Phase Synthesis Techniques ; Peptides/chemistry ; Peptide Library ; Lysine/chemistry ; Arginine
Chemical Substances	Peptides ; Peptide Library ; Lysine (K3Z4F929H6) ; Arginine (94ZLA3W45F)
Language	English
Publishing date	2023-12-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1024041-x
ISSN	1520-4812 ; 1043-1802
ISSN (online)	1520-4812
ISSN	1043-1802
DOI	10.1021/acs.bioconjchem.3c00381
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3400: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Theranostic PSMA ligands with optimized backbones for intraoperative multimodal imaging and photodynamic therapy of prostate cancer.

Derks, Yvonne H W / van Lith, Sanne A M / Amatdjais-Groenen, Helene I V / Wouters, Lieke W M / Kip, Annemarie / Franssen, Gerben M / Laverman, Peter / Löwik, Dennis W P M / Heskamp, Sandra / Rijpkema, Mark

European journal of nuclear medicine and molecular imaging

2022 Volume 49, Issue 7, Page(s) 2425–2435

Abstract: Introduction: The first generation ligands for prostate-specific membrane antigen (PSMA)-targeted radio- and fluorescence-guided surgery followed by adjuvant photodynamic therapy (PDT) have already shown the potential of this approach. Here, we ... ...

Abstract	Introduction: The first generation ligands for prostate-specific membrane antigen (PSMA)-targeted radio- and fluorescence-guided surgery followed by adjuvant photodynamic therapy (PDT) have already shown the potential of this approach. Here, we developed three new photosensitizer-based dual-labeled PSMA ligands by crucial modification of existing PSMA ligand backbone structures (PSMA-1007/PSMA-617) for multimodal imaging and targeted PDT of PCa. Methods: Various new PSMA ligands were synthesized using solid-phase chemistry and provided with a DOTA chelator for Results: In order to synthesize the new dual-labeled ligands, we modified the PSMA peptide linker by substitution of a glutamic acid into a lysine residue, providing a handle for conjugation of multiple functional moieties. Ligand optimization showed that the new backbone structure leads to high-affinity PSMA ligands (all IC Conclusion: The new high-affinity dual-labeled PSMA-targeting ligands with optimized backbone compositions showed increased tumor targeting and enabled multimodal image-guided PCa surgery combined with targeted photodynamic therapy.
MeSH term(s)	Animals ; Antigens, Surface/metabolism ; Cell Line, Tumor ; Glutamate Carboxypeptidase II/metabolism ; Humans ; Ligands ; Male ; Mice ; Mice, Nude ; Multimodal Imaging ; Photochemotherapy ; Photosensitizing Agents/therapeutic use ; Precision Medicine ; Prostatic Neoplasms/drug therapy ; Prostatic Neoplasms/therapy ; Tissue Distribution
Chemical Substances	Antigens, Surface ; Ligands ; Photosensitizing Agents ; Glutamate Carboxypeptidase II (EC 3.4.17.21)
Language	English
Publishing date	2022-01-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	8236-3
ISSN	1619-7089 ; 0340-6997 ; 1619-7070
ISSN (online)	1619-7089
ISSN	0340-6997 ; 1619-7070
DOI	10.1007/s00259-022-05685-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1227: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito