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  1. Article ; Online: Draft genome sequences of two thermophilic, spore-forming

    Lücking, Genia / Albrecht, Klement / Märtlbauer, Erwin / Schauer, Kristina

    Microbiology resource announcements

    2023  Volume 13, Issue 1, Page(s) e0089623

    Abstract: The presence of thermophilic spore-forming bacteria is challenging in industrial food processing. The presented genome sequences ... ...

    Abstract The presence of thermophilic spore-forming bacteria is challenging in industrial food processing. The presented genome sequences of
    Language English
    Publishing date 2023-12-13
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.00896-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR

    Maier, Christopher / Hofmann, Katharina / Huptas, Christopher / Scherer, Siegfried / Wenning, Mareike / Lücking, Genia

    Applied microbiology and biotechnology. 2021 Feb., v. 105, no. 4

    2021  

    Abstract: The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification ... ...

    Abstract The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in raw milk, we developed two triplex qPCR assays taking into account species-dependent differences in AprX activity. Besides five species-specific hydrolysis probes, targeting the aprX gene, a universal rpoB probe was included in the assay to determine the total Pseudomonas counts. For all six probes, linear regression lines between Cq value and target DNA concentration were obtained in singleplex as well as in multiplex approaches, yielding R² values of > 0.975 and amplification efficiencies of 85–97%. Moreover, high specificity was determined using genomic DNA of 75 Pseudomonas strains, assigned to 57 species, and 40 other bacterial species as templates in the qPCR. Quantification of the target species and total Pseudomonas counts resulted in linear detection ranges of approx. 10³–10⁷ cfu/ml, which correspond well to common Pseudomonas counts in raw milk. Application of the assay using 60 raw milk samples from different dairies showed good agreement of total Pseudomonas counts calculated by qPCR with cell counts derived from cultivation. Furthermore, a remarkably high variability regarding the species composition was observed for each milk sample, whereby P. lundensis and P. proteolytica/P. gessardii were the predominant species detected. KEY POINTS: • Multiplex qPCR for quantification of seven proteolytic Pseudomonas species and total Pseudomonas counts in raw milk • High specificity and sensitivity via hydrolysis probes against aprX and rpoB • Rapid method to determine Pseudomonas contamination in raw milk and predict spoilage potential
    Keywords DNA ; UHT treatment ; biotechnology ; genes ; heat stability ; hydrolysis ; microbiology ; milk ; proteolysis ; rapid methods ; raw milk ; regression analysis ; species diversity ; spoilage
    Language English
    Dates of publication 2021-02
    Size p. 1693-1708.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-021-11109-0
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 79/727: Show issues

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  3. Article ; Online: Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR.

    Maier, Christopher / Hofmann, Katharina / Huptas, Christopher / Scherer, Siegfried / Wenning, Mareike / Lücking, Genia

    Applied microbiology and biotechnology

    2021  Volume 105, Issue 4, Page(s) 1693–1708

    Abstract: The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification ... ...

    Abstract The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in raw milk, we developed two triplex qPCR assays taking into account species-dependent differences in AprX activity. Besides five species-specific hydrolysis probes, targeting the aprX gene, a universal rpoB probe was included in the assay to determine the total Pseudomonas counts. For all six probes, linear regression lines between C
    MeSH term(s) Animals ; Hot Temperature ; Milk/metabolism ; Peptide Hydrolases/metabolism ; Proteolysis ; Pseudomonas/genetics ; Pseudomonas/metabolism
    Chemical Substances Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2021-02-01
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-021-11109-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2229: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Genetic Organization of the

    Maier, Christopher / Huptas, Christopher / von Neubeck, Mario / Scherer, Siegfried / Wenning, Mareike / Lücking, Genia

    Frontiers in microbiology

    2020  Volume 11, Page(s) 1190

    Abstract: ... ...

    Abstract Psychrotolerant
    Language English
    Publishing date 2020-06-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.01190
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Reprogramming Human Siderocalin To Neutralize Petrobactin, the Essential Iron Scavenger of Anthrax Bacillus.

    Dauner, Martin / Eichinger, Andreas / Lücking, Genia / Scherer, Siegfried / Skerra, Arne

    Angewandte Chemie (International ed. in English)

    2018  Volume 57, Issue 44, Page(s) 14619–14623

    Abstract: Bacillus anthracis owes its pronounced virulence-apart from specific toxins-to a twofold import mechanism for ... ...

    Abstract Bacillus anthracis owes its pronounced virulence-apart from specific toxins-to a twofold import mechanism for Fe
    MeSH term(s) Bacillus anthracis/metabolism ; Bacterial Proteins/metabolism ; Benzamides/metabolism ; Carrier Proteins/metabolism ; Humans ; Iron/metabolism ; Ligands
    Chemical Substances Bacterial Proteins ; Benzamides ; Carrier Proteins ; LCN2 protein, human ; Ligands ; petrobactin ; Iron (E1UOL152H7)
    Language English
    Publishing date 2018-10-02
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.201807442
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  6. Book ; Audio / Video ; Online: Pseudomonaden in Rohmilch

    Franz, Charles M. A. P. / Gieschler, Stefanie / Hofmann, Katharina / Hille, Frank / Fiedler, Gregor / Lücking, Genia / Wenning, Mareike / Böhnlein, Christina

    Eintragsquellen und Möglichkeiten zur Biokontrolle mit Bakteriophagen

    2022  

    Keywords Text ; ddc:610
    Language German
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Book ; Audio / Video ; Online: Pseudomonaden in Rohmilch

    Gieschler, Stefanie / Hoffmann, Katharina / Lücking, Genia / Wenning, Mareike / Hetzer, Birgit / Böhnlein, Christina / Scherer, Siegfried / Franz, Charles M. A. P.

    (k)ein Problem für deutsche Milcherzeuger

    2022  

    Keywords Text ; ddc:610
    Language German
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Book ; Online ; Thesis: Molecular characterization of cereulide synthesis in emetic Bacillus cereus

    Lücking, Genia [Verfasser]

    2009  

    Author's details Genia Lücking
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  9. Book ; Audio / Video ; Online: Hofseitige Einflussfaktoren auf die Zusammensetzung des Rohmilchmikrobioms

    Wenning, Mareike / Siebert, Annemarie / Fiedler, Gregor / Hofmann, Katharina / Gieschler, Stefanie / Lücking, Genia / Walte, Hans-Georg / Böhnlein, Christina / Franz, Charles M. A. P. / Scherer, Siegfried

    2022  

    Keywords Text ; ddc:610
    Language German
    Publishing country de
    Document type Book ; Audio / Video ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  10. Article ; Online: Transcriptional kinetic analyses of cereulide synthetase genes with respect to growth, sporulation and emetic toxin production in Bacillus cereus.

    Dommel, Monica K / Lücking, Genia / Scherer, Siegfried / Ehling-Schulz, Monika

    Food microbiology

    2011  Volume 28, Issue 2, Page(s) 284–290

    Abstract: In light of the increasing number of serious food borne outbreaks caused by emetic Bacillus cereus, a better understanding of the cereulide synthetase (ces) gene expression and toxin synthesis is required. Here, the relative expression levels of three ... ...

    Abstract In light of the increasing number of serious food borne outbreaks caused by emetic Bacillus cereus, a better understanding of the cereulide synthetase (ces) gene expression and toxin synthesis is required. Here, the relative expression levels of three ces genes (cesP, cesA and cesB) were investigated using quantitative real-time reverse transcription PCR in relation to growth, degree of sporulation and toxin production of the emetic reference strain B. cereus F4810/72 and the weakly emetic strain IH41385. The strict co-transcription of all three genes confirmed the operon structure of the ces gene cluster responsible for cereulide formation. ces transcription turned out to be highly temporal and tightly regulated; ces mRNA was only detectable during mid to late exponential growth in both strains. The low toxigenic potential of the weakly emetic strain IH41385 correlated well with its respective ces transcripts, showing reduced activity at a transcriptional level. Two non-sporulating mutants (F4810/72Δspo0A and F4810/72INsigH) demonstrated that cereulide synthesis is part of the Spo0A regulon but independent of later sporulation processes. Besides strain specific intrinsic factors, ces transcription was found to be significantly influenced by the cellular growth state as well as by extrinsic abiotic factors, like salt. An increase of sodium chloride in the media resulted in lower ces transcription and coincided with lower cereulide toxin levels. Interestingly, at 25 gl(-1) NaCl, toxin levels were already reduced without strongly affecting the growth of B. cereus, indicating an inhibitory effect of NaCl on cereulide biosynthesis independent of growth. This illustrates that ces gene expression and toxicity cannot be predicted solely from growth rates or cell numbers, but is influenced by complex interactions of various intrinsic as well as extrinsic factors, which remain to be clarified in detail.
    MeSH term(s) Bacillus cereus/genetics ; Bacillus cereus/metabolism ; Bacterial Toxins/biosynthesis ; Bacterial Toxins/genetics ; Depsipeptides/genetics ; Depsipeptides/metabolism ; Food Microbiology ; Humans ; Ligases/genetics ; Ligases/metabolism ; Promoter Regions, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; Spores, Bacterial/genetics ; Spores, Bacterial/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
    Chemical Substances Bacterial Toxins ; Depsipeptides ; Transcription Factors ; cereulide ; Ligases (EC 6.-)
    Language English
    Publishing date 2011-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 50892-5
    ISSN 1095-9998 ; 0740-0020
    ISSN (online) 1095-9998
    ISSN 0740-0020
    DOI 10.1016/j.fm.2010.07.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4667: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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