LIVIVO - Search results -

Search results

Result 1 - 10 of total 23

Search options

Article ; Online: Bordetella trematum

Wong, Crystal / Calungsud, Lynna Grace / La, My-Van

Access microbiology

2023 Volume 5, Issue 7

Abstract: Introduction: Bordetella trematum: Case presentation: Case presentation.: Conclusion: ... ...

Abstract	Introduction: Bordetella trematum Case presentation: Case presentation. Conclusion: Invasive
Language	English
Publishing date	2023-07-13
Publishing country	England
Document type	Case Reports
ISSN	2516-8290
ISSN (online)	2516-8290
DOI	10.1099/acmi.0.000602.v3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: A Study of Analytical and Clinical Sensitivity of Aptima SARS-CoV-2 Assay (Hologic) and Proposals of Complementary Tests for SARS-CoV-2 Detection in Low Viral Load Specimens

La, My-Van / Koo, Seok Hwee / Jiang, Boran / Heng, Ying Xuan / Tan, Thean Yen

Current microbiology. 2022 Jan., v. 79, no. 1

2022

Abstract: Early and accurate detection of SARS-CoV-2 is important for diagnosis and transmission control. The use of high-throughput and automated testing allows laboratories to better deliver diagnostic testing given manpower and resource limitations. We ... ...

Abstract	Early and accurate detection of SARS-CoV-2 is important for diagnosis and transmission control. The use of high-throughput and automated testing allows laboratories to better deliver diagnostic testing given manpower and resource limitations. We validated the clinical and analytical performance of the Hologic Panther Aptima SARS-CoV-2 assay with an emphasis on detection of specimens with low viral loads. The clinical performance was evaluated using 245 clinical specimens, against a comparator PCR-based laboratory developed test (LDT). The analytical performance was determined by replicate testing of contrived samples in a ten-fold dilution series (CT values 32–42, based on LDT). The Aptima assay had 96.7% overall percent agreement, 100% negative percent agreement and 88.1% positive percent agreement. It was able to consistently detect SARS-CoV-2 in contrived samples with CT = 32 by LDT (calculated 2354 copies/mL). The 95% limit of detection of the Aptima assay was estimated to be at LDT CT = 33 (equivalent to 870 copies/mL). The relative light units (RLU) × 1000 for 52 true positive clinical specimens was 962.2 ± 181.5, and that for the 186 true negative specimens was 264.6 ± 14.3. The Aptima assay was a reliable method with a high overall percent agreement against our comparator LDT. We propose that samples reported as negative by the Aptima assay with RLU > 350 be tested by a secondary method, in order to improve detection of samples with very low viral loads.
Keywords	Severe acute respiratory syndrome coronavirus 2 ; detection limit ; microbiology ; polymerase chain reaction ; viral load
Language	English
Dates of publication	2022-01
Size	p. 29.
Publishing place	Springer US
Document type	Article
ZDB-ID	134238-1
ISSN	1432-0991 ; 0343-8651
ISSN (online)	1432-0991
ISSN	0343-8651
DOI	10.1007/s00284-021-02730-3
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 80/708: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Development of a rapid multiplex PCR assay for the detection of common pathogens associated with community-acquired pneumonia.

Koo, Seok Hwee / Jiang, Boran / Lim, Pei Qi / La, My-Van / Tan, Thean Yen

Transactions of the Royal Society of Tropical Medicine and Hygiene

2021 Volume 115, Issue 12, Page(s) 1450–1455

Abstract: Background: Community-acquired pneumonia (CAP) is one of the most common infectious diseases and is a significant cause of mortality and morbidity globally. A microbial cause was not determined in a sizable percentage of patients with CAP; there are ... ...

Abstract	Background: Community-acquired pneumonia (CAP) is one of the most common infectious diseases and is a significant cause of mortality and morbidity globally. A microbial cause was not determined in a sizable percentage of patients with CAP; there are increasing data to suggest regional differences in bacterial aetiology. We devised a multiplex real-time PCR assay for detecting four microorganisms (Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae and Burkholderia pseudomallei) of relevance to CAP infections in Asia. Methods: Analytical validation was accomplished using bacterial isolates (n=10-33 of each target organism for analytical sensitivity and n=117 for analytical sensitivity) and clinical validation using 58 culture-positive respiratory tract specimens. Results: The qPCR assay exhibited 100% analytical sensitivity and analytical specificity, and 100% clinical sensitivity and 94-100% clinical specificity. The limit of detection and efficiency for the multiplex PCR assay were 3-33 CFU/mL and 93-110%, respectively. The results showed that the PCR-based method had higher sensitivity than traditional culture-based methods. The assay also demonstrated an ability to semiquantify bacterial loads. Conclusion: We have devised a reliable laboratory-developed multiplex qPCR assay, with a turnaround time of within one working day, for detection of four clinically important CAP-associated microorganisms in Asia. The availability of a test with improved diagnostic capabilities potentially leads to an informed choice of antibiotic usage and appropriate management of the patient to achieve a better treatment outcome and financial savings.
MeSH term(s)	Community-Acquired Infections/diagnosis ; Humans ; Multiplex Polymerase Chain Reaction ; Pneumonia, Bacterial/diagnosis ; Sensitivity and Specificity ; Streptococcus pneumoniae/genetics
Language	English
Publishing date	2021-05-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	441375-1
ISSN	1878-3503 ; 0035-9203
ISSN (online)	1878-3503
ISSN	0035-9203
DOI	10.1093/trstmh/trab079
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Un I Zs.257: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A Study of Analytical and Clinical Sensitivity of Aptima SARS-CoV-2 Assay (Hologic) and Proposals of Complementary Tests for SARS-CoV-2 Detection in Low Viral Load Specimens.

La, My-Van / Koo, Seok Hwee / Jiang, Boran / Heng, Ying Xuan / Tan, Thean Yen

Current microbiology

2021 Volume 79, Issue 1, Page(s) 29

Abstract	Early and accurate detection of SARS-CoV-2 is important for diagnosis and transmission control. The use of high-throughput and automated testing allows laboratories to better deliver diagnostic testing given manpower and resource limitations. We validated the clinical and analytical performance of the Hologic Panther Aptima SARS-CoV-2 assay with an emphasis on detection of specimens with low viral loads. The clinical performance was evaluated using 245 clinical specimens, against a comparator PCR-based laboratory developed test (LDT). The analytical performance was determined by replicate testing of contrived samples in a ten-fold dilution series (C
MeSH term(s)	COVID-19 ; Humans ; Molecular Diagnostic Techniques ; RNA, Viral ; SARS-CoV-2 ; Sensitivity and Specificity ; Viral Load
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-12-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	134238-1
ISSN	1432-0991 ; 0343-8651
ISSN (online)	1432-0991
ISSN	0343-8651
DOI	10.1007/s00284-021-02730-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1446: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Development and evaluation of a multiplex real-time PCR for the detection of IMP, VIM, and OXA-23 carbapenemase gene families on the BD MAX open system.

Teo, Jeanette W P / La, My-Van / Lin, Raymond T P

Diagnostic microbiology and infectious disease

2016 Volume 86, Issue 4, Page(s) 358–361

Abstract: A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all ... ...

Abstract	A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like.
MeSH term(s)	Bacterial Proteins/analysis ; Bacterial Proteins/genetics ; Bacteriological Techniques/methods ; Enterobacteriaceae/enzymology ; Enterobacteriaceae/genetics ; Enterobacteriaceae Infections/microbiology ; Humans ; Multiplex Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/methods ; beta-Lactamases/analysis ; beta-Lactamases/genetics
Chemical Substances	Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
Language	English
Publishing date	2016-12
Publishing country	United States
Document type	Evaluation Studies ; Journal Article
ZDB-ID	604920-5
ISSN	1879-0070 ; 0732-8893
ISSN (online)	1879-0070
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2016.08.019
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1845: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Colistin and Polymyxin B Susceptibility Testing for Carbapenem-Resistant and

Chew, Ka Lip / La, My-Van / Lin, Raymond T P / Teo, Jeanette W P

Journal of clinical microbiology

2017 Volume 55, Issue 9, Page(s) 2609–2616

Abstract: Colistin and polymyxin B remain part of the last line of antibiotics for multidrug-resistant Gram-negative bacteria, such as carbapenem- ... ...

Abstract	Colistin and polymyxin B remain part of the last line of antibiotics for multidrug-resistant Gram-negative bacteria, such as carbapenem-resistant
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Carbapenem-Resistant Enterobacteriaceae/drug effects ; Carbapenem-Resistant Enterobacteriaceae/genetics ; Colistin/pharmacology ; Drug Resistance, Multiple, Bacterial/genetics ; Escherichia coli Proteins/genetics ; Microbial Sensitivity Tests ; Plasmids/genetics ; Polymyxin B/pharmacology
Chemical Substances	Anti-Bacterial Agents ; Escherichia coli Proteins ; MCR-1 protein, E coli ; Polymyxin B (1404-26-8) ; Colistin (Z67X93HJG1)
Language	English
Publishing date	2017
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.00268-17
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1188: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Prevalence and antibiotic susceptibility of colistin-resistance gene (mcr-1) positive Enterobacteriaceae in stool specimens of patients attending a tertiary care hospital in Singapore.

La, My-Van / Lee, Biondi / Hong, Brian Z M / Yah, Jing Yan / Koo, Seok-Hwee / Jiang, Boran / Ng, Lily S Y / Tan, Thean-Yen

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2019 Volume 85, Page(s) 124–126

Abstract: Objectives: The aim of this study was to determine the prevalence of the colistin-resistance gene (mcr-1) and the antibiotic-susceptibility profile of mcr-1 positive, colistin-resistant isolates in stool specimens of patients attending a tertiary care ... ...

Abstract	Objectives: The aim of this study was to determine the prevalence of the colistin-resistance gene (mcr-1) and the antibiotic-susceptibility profile of mcr-1 positive, colistin-resistant isolates in stool specimens of patients attending a tertiary care hospital in Singapore. Methods: 201 diarrheal stool specimens of patients attending the Changi General Hospital between May to August 2017 were collected and screened for the presence of mcr-1 by culture and molecular methods. Antibiotic-susceptibility profile of mcr-1 positive isolates was determined using the polymyxin B and colistin E-tests and the VITEK 2 system. Results: We observed an unexpectedly high prevalence of mcr-1 in patients attending a tertiary care hospital in Singapore, i.e 6.0% and 8.0% estimated by stool culture and direct stool PCR, respectively. The mcr-1 gene was detected predominantly in Escherichia coli. Antibiotic-susceptibility testing on 12 mcr-1 positive Enterobacteriaceae isolates revealed variable susceptibility profiles with no detection of carbapenem-resistant Enterobacteriaceae. Conclusions: This is the first report of the prevalence of human faecal carriage of mcr-1 in Singapore. Our findings highlight the potential risk of mcr-1 spread among our patient cohort. The mcr-1 gene detection combined with the detection of other resistance gene targets of clinical importance is recommended to pre-empt the spread mcr-1 in our patients.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Colistin/pharmacology ; Drug Resistance, Bacterial/genetics ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/genetics ; Enterobacteriaceae/isolation & purification ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Feces/microbiology ; Genes, Bacterial ; Humans ; Polymyxin B/pharmacology ; Singapore ; Tertiary Care Centers
Chemical Substances	Anti-Bacterial Agents ; Polymyxin B (J2VZ07J96K) ; Colistin (Z67X93HJG1)
Language	English
Publishing date	2019-05-30
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2019.05.029
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4516: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Draft Genome Sequence of Singapore

Koh, Tse H / Abdul Rahman, Nurdyana Binte / Teo, Jeanette W P / La, My-Van / Periaswamy, Balamurugan / Chen, Swaine L

Genome announcements

2018 Volume 6, Issue 1

Abstract: We sequenced the ... ...

Abstract	We sequenced the first
Language	English
Publishing date	2018-01-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	2704277-7
ISSN	2169-8287
ISSN	2169-8287
DOI	10.1128/genomeA.01377-17
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Emergence of a New Delhi metallo-β-lactamase-1-producing Pseudomonas aeruginosa in Singapore.

Teo, Jeanette W P / La, My-Van / Jureen, Roland / Lin, Raymond T P

Emerging microbes & infections

2015 Volume 4, Issue 11, Page(s) e72

MeSH term(s)	Aged, 80 and over ; Anti-Bacterial Agents/pharmacology ; Cross Infection/microbiology ; Cross Infection/transmission ; Drug Resistance, Multiple, Bacterial ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Pseudomonas Infections/epidemiology ; Pseudomonas Infections/microbiology ; Pseudomonas Infections/transmission ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/enzymology ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/isolation & purification ; Singapore/epidemiology ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6) ; beta-lactamase NDM-1 (EC 3.5.2.6)
Language	English
Publishing date	2015-11-25
Publishing country	United States
Document type	Case Reports ; Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2681359-2
ISSN	2222-1751 ; 2222-1751
ISSN (online)	2222-1751
ISSN	2222-1751
DOI	10.1038/emi.2015.72
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Putative Integrative Mobile Elements That Exploit the Xer Recombination Machinery Carrying

Koh, Tse H / Rahman, Nurdyana Binte Abdul / Teo, Jeanette W P / La, My-Van / Periaswamy, Balamurugan / Chen, Swaine L

Antimicrobial agents and chemotherapy

2017 Volume 62, Issue 1

Abstract: Whole-genome sequencing was performed on 16 isolates of the carbapenemase- ... ...

Abstract	Whole-genome sequencing was performed on 16 isolates of the carbapenemase-producing
MeSH term(s)	Bacterial Proteins/genetics ; Drug Resistance, Bacterial/genetics ; Enterobacter cloacae/drug effects ; Enterobacter cloacae/genetics ; Enterobacter cloacae/isolation & purification ; Genome, Bacterial/genetics ; Humans ; Interspersed Repetitive Sequences/genetics ; Multilocus Sequence Typing ; Singapore ; Whole Genome Sequencing ; beta-Lactamases/genetics
Chemical Substances	Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
Language	English
Publishing date	2017-12-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.01542-17
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 809: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Einzelsignatur: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito