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Article: Effect of Stool Sampling on a Routine Clinical Method for the Quantification of Six Short Chain Fatty Acids in Stool Using Gas Chromatography-Mass Spectrometry.

Mahdi, Tarek / Desmons, Aurore / Krasniqi, Pranvera / Lacorte, Jean-Marc / Kapel, Nathalie / Lamazière, Antonin / Fourati, Salma / Eguether, Thibaut

Microorganisms

2024 Volume 12, Issue 4

Abstract: Short chain fatty acids (SCFAs) are primarily produced in the caecum and proximal colon via the bacterial fermentation of undigested carbohydrates that have avoided digestion in the small intestine. Increasing evidence supports the critical role that ... ...

Abstract	Short chain fatty acids (SCFAs) are primarily produced in the caecum and proximal colon via the bacterial fermentation of undigested carbohydrates that have avoided digestion in the small intestine. Increasing evidence supports the critical role that SCFAs play in health and homeostasis. Microbial SCFAs, namely butyric acid, serve as a principal energy source for colonocytes, and their production is essential for gut integrity. A direct link between SCFAs and some human pathological conditions, such as inflammatory bowel disease, irritable bowel syndrome, diarrhea, and cancer, has been proposed. The direct measurement of SCFAs in feces provides a non-invasive approach to demonstrating connections between SCFAs, microbiota, and metabolic diseases to estimate their potential applicability as meaningful biomarkers of intestinal health. This study aimed to adapt a robust analytical method (liquid-liquid extraction, followed by isobutyl chloroformate derivatization and GC-MS analysis), with comparable performances to methods from the literature, and to use this tool to tackle the question of pre-analytical conditions, namely stool processing. We focused on the methodology of managing stool samples before the analysis (fresh stool or dilution in either ethanol/methanol, lyophilized stool, or RNAlater
Language	English
Publishing date	2024-04-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms12040828
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Article ; Online: Detection of RAS mutations in circulating tumor cells: applications in colorectal cancer and prospects.

Denis, Jérôme Alexandre / Lacorte, Jean-Marc

Annales de biologie clinique

2017 Volume 75, Issue 6, Page(s) 607–618

Abstract: The somatic mutations in the RAS genes (KRAS and NRAS) are widely associated with non-response to immunotherapies targeting the epidermal growth factor receptor in metastatic colorectal cancer. The detection of these mutations is carried out from tissue ... ...

Title translation	Détection des mutations RAS dans les cellules tumorales circulantes : applications au cancer colorectal et perspectives.
Abstract	The somatic mutations in the RAS genes (KRAS and NRAS) are widely associated with non-response to immunotherapies targeting the epidermal growth factor receptor in metastatic colorectal cancer. The detection of these mutations is carried out from tissue biopsies and become mandatory to prescribe these treatments. Nethertheless, this analysis is not possible in about 25% of cases and the development of alternative methods is therefore required. Among them, the search for mutations directly in the blood of patients are promising approaches. Circulating tumor cells (CTCs) represent a particularly relevant direct target. These cells, some of them have inducing-metastasis capabilities, have been able to detach themselves from the primary tumor, then migrate and finally enter into the bloodstream. In this sense, they are particularly resistant to physical-chemical and immunological constraints used by the organism to prevent their dissemination. Consequently, they represent a particularly valuable source of information on the most aggressive tumor cells. As a corollary, these cells are very rare requiring particularly highly performant technologies to be detected. In this presentation, we focus mainly on the molecular methods used to detect these mutated RAS cells by analyzing the performance of a solution based on a filtration device followed by detection with digital PCR. Finally, we will discuss the biological significance of these cells before highlighting prospects in colorectal cancer but also in other cancers.
MeSH term(s)	Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; DNA Mutational Analysis/methods ; DNA Mutational Analysis/trends ; Genetic Testing/trends ; Humans ; Medical Oncology/trends ; Mutation ; Neoplastic Cells, Circulating/metabolism ; Neoplastic Cells, Circulating/pathology ; Proto-Oncogene Proteins p21(ras)/genetics ; Proto-Oncogene Proteins p21(ras)/metabolism
Chemical Substances	Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2)
Language	English
Publishing date	2017-12-01
Publishing country	France
Document type	Journal Article ; Review
ZDB-ID	2023584-7
ISSN	1950-6112 ; 0003-3898
ISSN (online)	1950-6112
ISSN	0003-3898
DOI	10.1684/abc.2017.1304
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Article ; Online: Circulating Apolipoprotein B-48 as a Biomarker of Parenteral Nutrition Dependence in Adult Patients with Short Bowel Syndrome.

Fourati, Salma / Hamon, Annick / Daclat, Rita / Salem, Joe-Elie / Peoc'h, Katell / Le Beyec, Johanne / Joly, Francisca / Lacorte, Jean-Marc

Nutrients

2023 Volume 15, Issue 18

Abstract: Short bowel syndrome (SBS) is a rare but serious condition that may lead to chronic intestinal failure. Citrulline concentrations are currently used to reflect the residual intestinal mass in patients with SBS, although this method has several ... ...

Abstract	Short bowel syndrome (SBS) is a rare but serious condition that may lead to chronic intestinal failure. Citrulline concentrations are currently used to reflect the residual intestinal mass in patients with SBS, although this method has several limitations. In a cohort of patients with SBS, we quantified apolipoprotein B-48 (ApoB-48), which is exclusively synthesized by enterocytes and secreted associated with dietary lipids and investigated the relationship between ApoB-48 and clinical and biological data as well as PN dependence. A total of 51 adult patients were included, 36 of whom were PN-dependent. We found a robust positive correlation between circulating ApoB-48 and residual small bowel length, which was also found in the subgroup of patients with jejunocolic anastomosis. Fasting ApoB-48 levels were significantly lower in PN-dependent patients than in PN-weaned patients and negatively correlated with parenteral nutrition dependence. Our results suggest that ApoB-48 could be proposed as a marker of intestinal absorptive function and could be an interesting follow-up marker in patients with SBS.
Language	English
Publishing date	2023-09-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu15183982
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Article ; Online: Interleukin-6 chemiluminescent immunoassay on Lumipulse G600 II: analytical evaluation and comparison with three other laboratory analyzers.

Glady, Ludovic / Lavaux, Thomas / Charchour, Rim / Lacorte, Jean-Marc / Lessinger, Jean-Marc

Clinical chemistry and laboratory medicine

2019 Volume 58, Issue 10, Page(s) e229–e231

MeSH term(s)	Clinical Laboratory Techniques/instrumentation ; Humans ; Immunoassay/instrumentation ; Interleukin-6/blood ; Limit of Detection ; Luminescence ; Reproducibility of Results
Chemical Substances	Interleukin-6
Language	English
Publishing date	2019-12-20
Publishing country	Germany
Document type	Comparative Study ; Letter
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2019-1145
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Article ; Online: Influence of K656N Polymorphism of the Leptin Receptor Gene on Obesity-Related Traits in Nondiabetic Afro-Caribbean Individuals.

Foucan, Lydia / Bassien-Capsa, Valérie / Rambhojan, Christine / Lacorte, Jean-Marc / Larifla, Laurent

Metabolic syndrome and related disorders

2019 Volume 17, Issue 4, Page(s) 197–203

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Adult ; Africa ; Alleles ; Blacks ; Body Mass Index ; Caribbean Region ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Genotype ; Guadeloupe/epidemiology ; Humans ; Leptin/blood ; Linear Models ; Male ; Metabolic Syndrome/ethnology ; Metabolic Syndrome/genetics ; Middle Aged ; Obesity/ethnology ; Obesity/genetics ; Obesity, Abdominal/genetics ; Overweight/genetics ; Phenotype ; Polymorphism, Single Nucleotide ; Receptors, Leptin/genetics ; Regression Analysis ; Waist Circumference
Chemical Substances	LEPR protein, human ; Leptin ; Receptors, Leptin
Language	English
Publishing date	2019-02-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2151220-6
ISSN	1557-8518 ; 1540-4196
ISSN (online)	1557-8518
ISSN	1540-4196
DOI	10.1089/met.2018.0133
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Article ; Online: Serum vascular endothelial growth factor is associated with cardiovascular involvement and response to therapy in Erdheim-Chester disease.

Roeser, Anais / Bravetti, Marine / Dong, Lida / Azoulay, Levi-Dan / Charlotte, Frederic / Miyara, Makoto / Ghillani-Dalbin, Pascale / Emile, Jean-Francois / El Kouari, Fadwa / Ouni, Hamza / Lacorte, Jean-Marc / Brocheriou, Isabelle / Amoura, Zahir / Cohen-Aubart, Fleur / Haroche, Julien

Haematologica

2023 Volume 108, Issue 2, Page(s) 513–521

Abstract: Erdheim-Chester disease (ECD) is a rare histiocytosis, considered to be an inflammatory myeloid neoplasm. Tropism for specific involvements of the disease remains unexplained. Vascular endothelial growth factor-A (VEGF) is implicated in cancer ... ...

Abstract	Erdheim-Chester disease (ECD) is a rare histiocytosis, considered to be an inflammatory myeloid neoplasm. Tropism for specific involvements of the disease remains unexplained. Vascular endothelial growth factor-A (VEGF) is implicated in cancer pathophysiology and mutations of the RAS oncogene have been shown to induce upregulation of VEGF gene expression. We therefore hypothesized that VEGF might play a particular role in ECD pathophysiology. We conducted a retrospective, single-center study to assess serum VEGF (sVEGF) concentrations and determine whether they were associated with the characteristics of ECD patients, and to determine whether VEGF was expressed by histiocytes. We evaluated 247 ECD patients, 53.4% of whom had sVEGF levels above the normal range (>500 pg/mL). Patients with high sVEGF levels more frequently had cardiac and vascular involvement (58.3% vs. 41.4%, P=0.008 and 70.5% vs. 48.3%, P=0.0004, respectively). In treatment-naïve patients (n=135), the association of C-reactive protein >5 mg/L and sVEGF >500 pg/mL was strongly associated with vascular involvement (odds ratio=5.54 [95% confidence interval: 2.39-13.62], P<0.001), and independently associated with cardiac involvement (odds ratio=3.18 [95% confidence interval: 1.34-7.83], P=0.010) after adjustment for the presence of the BRAF V600E mutation. Changes in sVEGF concentration on treatment were associated with a response of cardiac involvement on consecutive cardiac magnetic resonance images. All histological samples analyzed (n=24) displayed histiocytes with intracytoplasmic expression of VEGF, which was moderate to high in more than 90% of cases. Our study suggests a role for VEGF in cardiac and vascular involvement in ECD.
MeSH term(s)	Humans ; Vascular Endothelial Growth Factor A/genetics ; Retrospective Studies ; Erdheim-Chester Disease/diagnosis ; Erdheim-Chester Disease/drug therapy ; Erdheim-Chester Disease/genetics ; Vascular Endothelial Growth Factors ; Neoplasms
Chemical Substances	Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors
Language	English
Publishing date	2023-02-01
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2333-4
ISSN	1592-8721 ; 0017-6567 ; 0390-6078
ISSN (online)	1592-8721
ISSN	0017-6567 ; 0390-6078
DOI	10.3324/haematol.2022.280755
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Article ; Online: Similar Gut Hormone Secretions Two Years After One Anastomosis Gastric Bypass and Roux-en-Y Gastric Bypass: a Pilot Study.

De Bandt, David / Rives-Lange, Claire / Frigout, Yvann / Bergerot, Damien / Blanchard, Anne / Le Gall, Maude / Lacorte, Jean-Marc / Chevallier, Jean-Marc / Czernichow, Sébastien / Poghosyan, Tigran / Carette, Claire / Le Beyec, Johanne

Obesity surgery

2022 Volume 32, Issue 3, Page(s) 757–762

Abstract: Objectives: One-anastomosis gastric bypass (OAGB) is as effective as Roux-en-Y gastric bypass (RYGB) regarding weight loss and diabetes remission. However, there are no data on gut hormone secretions after OAGB. The aim of this study was to compare ... ...

Abstract	Objectives: One-anastomosis gastric bypass (OAGB) is as effective as Roux-en-Y gastric bypass (RYGB) regarding weight loss and diabetes remission. However, there are no data on gut hormone secretions after OAGB. The aim of this study was to compare fasting and postprandial secretions of gut and pancreatic hormones in OAGB versus RYGB patients. Design and methods: Twenty-nine patients, 16 OAGB- and 13 RYGB-operated, underwent a liquid mixed-meal tolerance test at 2 years' post-surgery. Blood was sampled before and 15, 30, 60, 90, and 120 min after meal for plasma measurement of glucose, C-peptide, insulin, glucagon, GLP-1, GIP, GLP-2, PYY, and ghrelin. Results: Percentage of total weight loss 2 years post-surgery were -33.9 ± 1.8% for OAGB and -31.2 ± 1.6% for RYGB (p = 0.6). Four patients with persistent diabetes were excluded for further analysis. Fasting and postprandial glucose levels (peaks and area under curve values) were similar between groups. HOMA index was lower in the OAGB group (0.8 ± 0.1 vs 1.3 ± 0.2 in RYGB, p < 0.05). Levels of C-peptide (or insulin) measured at 30 min were significantly lower in OAGB vs RYGB patients (6.9 ± 0.5 vs 9.7 ± 1.1 µg/l, p < 0.05). No difference was observed between OAGB and RYGB groups for GLP-1, GLP-2, PYY, or ghrelin postprandial secretions, but GIP tended to be lower in OAGB vs RYGB patients (756 ± 155 vs 1100 ± 188 pg/ml for postprandial peak concentrations, p = 0.06). Conclusions: This is the first clinical study showing that OAGB procedure, like RYGB, results in high postprandial secretions of gut hormones, in particular GLP-1. Trial registration: Clinical Trials NCT03482895.
MeSH term(s)	Anastomosis, Roux-en-Y/methods ; Blood Glucose/analysis ; C-Peptide ; Gastric Bypass/methods ; Ghrelin ; Glucagon-Like Peptide 1 ; Glucose ; Humans ; Insulin ; Obesity, Morbid/surgery ; Pilot Projects ; Weight Loss
Chemical Substances	Blood Glucose ; C-Peptide ; Ghrelin ; Insulin ; Glucagon-Like Peptide 1 (89750-14-1) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2022-01-07
Publishing country	United States
Document type	Clinical Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1070827-3
ISSN	1708-0428 ; 0960-8923
ISSN (online)	1708-0428
ISSN	0960-8923
DOI	10.1007/s11695-021-05837-5
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Article ; Online: Could the chylomicron marker apoB48 be of value in the diagnosis of chylous effusions?

Lefrère, Bertrand / Sakka, Mehdi / Fourati, Salma / Levasseur, Antoine / Curis, Emmanuel / Cherfils, Corinne / Grès, Pierre / Guilbert, Zoé / Lacorte, Jean-Marc / Chenevière, Cristina / Bittar, Randa / Bonnefont-Rousselot, Dominique

Clinica chimica acta; international journal of clinical chemistry

2022 Volume 539, Page(s) 184–190

Abstract: Background: Chylous effusions such as chylothorax, chylopericardium and chylous ascites are marked by the abnormal presence of chylomicrons in serous membranes. These relatively rare situations are associated with high morbidity and mortality rates. ... ...

Abstract	Background: Chylous effusions such as chylothorax, chylopericardium and chylous ascites are marked by the abnormal presence of chylomicrons in serous membranes. These relatively rare situations are associated with high morbidity and mortality rates. Given that a macroscopic assessment of the fluid is insufficient, the current gold standard method for chylous effusion is the electrophoretic separation of lipoproteins. Serous effusions are most frequently assayed for triglycerides, with a diagnostic threshold varying between studies. The present study is the first to assess the value of the apolipoprotein B48, specific of the chylomicron, in the diagnosis of chylous effusions. Methods: A chemiluminescent sandwich enzyme immunoassay was used to measure levels of apoB48 in remnant samples of effusion fluid sent to our laboratory for chylomicron detection and lipid assays. The diagnostic values of apoB48 and triglyceride assays were compared with that of the gold standard method. Results: The triglyceride and apoB48 levels and the triglyceride/cholesterol ratio in the effusion fluid were significantly higher in patients with chylous effusion. The threshold values for apoB48 were respectively 2.45, 0.25 and 19.00 µg/mL for a maximal Youden index, a sensitivity > 95 %, and a specificity > 95 %. The apoB48 assay's diagnostic value might be at least as high as that of a triglyceride assay (area under the receiver operating characteristic curve [95 % confidence interval]: 0.84 [0.72, 0.96]) and 0.80 [0.67, 0.94], respectively). Conclusion: ApoB48 appears to be a promising marker for the diagnosis of chylous effusions; the putative diagnostic improvement must be confirmed in larger studies.
MeSH term(s)	Humans ; Chylomicrons ; Apolipoprotein B-48 ; Pleural Effusion/diagnosis ; Chylothorax/diagnosis ; Triglycerides
Chemical Substances	Chylomicrons ; Apolipoprotein B-48 ; Triglycerides
Language	English
Publishing date	2022-12-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2022.11.022
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Article ; Online: Detection of BRAFV600E by digital PCR on fine-needle aspirate enables rapid initiation of dabrafenib and trametinib in unresectable anaplastic thyroid carcinoma.

Buffet, Camille / Allard, Lucie / Guillerm, Erell / Ghander, Cécile / Mathy, Elise / Lussey-Lepoutre, Charlotte / Julien, Nicolas / Touma, Eliane / Quilhot, Pauline / Godiris-Petit, Gaelle / Lacorte, Jean-Marc / Leenhardt, Laurence / Denis, Jérôme Alexandre

European journal of endocrinology

2022 Volume 187, Issue 3, Page(s) K33–K38

Abstract: Introduction: Recently, targeted therapies using BRAFV600E and MEK inhibitors (dabrafenib and trametinib, respectively) have been recommended in BRAF-mutated anaplastic thyroid carcinoma (ATC). Considering the fast development of ATC, droplet digital ... ...

Abstract	Introduction: Recently, targeted therapies using BRAFV600E and MEK inhibitors (dabrafenib and trametinib, respectively) have been recommended in BRAF-mutated anaplastic thyroid carcinoma (ATC). Considering the fast development of ATC, droplet digital PCR (ddPCR) performed on fine-needle aspirate (FNA), which is a rapid, reliable, and low-cost method, appears interesting for the detection of BRAFV600E mutation in these patients and allows early initiation of targeted therapies. Results: In our two patients, both presenting extensive cervical masses inaccessible to surgery, ddPCR results were available in less than 24 h. Therefore, dabrafenib and trametinib were started only a few days after first contact. Conclusions: We suggest that ddPCR on FNA be used in non-resectable cervical masses for rapid BRAFV600E mutation detection in the hope that starting targeted therapies early might improve outcomes.
MeSH term(s)	Antineoplastic Combined Chemotherapy Protocols ; Humans ; Imidazoles ; Mutation ; Oximes ; Polymerase Chain Reaction/methods ; Proto-Oncogene Proteins B-raf/genetics ; Pyridones ; Pyrimidinones ; Thyroid Carcinoma, Anaplastic/drug therapy ; Thyroid Carcinoma, Anaplastic/genetics ; Thyroid Carcinoma, Anaplastic/pathology ; Thyroid Neoplasms/drug therapy ; Thyroid Neoplasms/genetics ; Thyroid Neoplasms/pathology
Chemical Substances	Imidazoles ; Oximes ; Pyridones ; Pyrimidinones ; trametinib (33E86K87QN) ; Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; dabrafenib (QGP4HA4G1B)
Language	English
Publishing date	2022-08-02
Publishing country	England
Document type	Journal Article
ZDB-ID	1183856-5
ISSN	1479-683X ; 0804-4643
ISSN (online)	1479-683X
ISSN	0804-4643
DOI	10.1530/EJE-22-0366
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Article ; Online: The Role of BEAMing and Digital PCR for Multiplexed Analysis in Molecular Oncology in the Era of Next-Generation Sequencing.

Denis, Jérôme Alexandre / Guillerm, Erell / Coulet, Florence / Larsen, Annette K / Lacorte, Jean-Marc

Molecular diagnosis & therapy

2017 Volume 21, Issue 6, Page(s) 587–600

Abstract: BEAMing polymerase chain reaction (PCR) and digital PCR (dPCR) are used for robust and accurate quantification of nucleic acids. These methods are particularly well suited for the identification of very small fractions (<1%) of variant copies such as the ...

Abstract	BEAMing polymerase chain reaction (PCR) and digital PCR (dPCR) are used for robust and accurate quantification of nucleic acids. These methods are particularly well suited for the identification of very small fractions (<1%) of variant copies such as the presence of mutant genes in a predominantly wild-type background. BEAMing and dPCR are increasingly used in diverse fields including bacteriology, virology, non-invasive prenatal testing, and oncology, in particular for the molecular analysis of liquid biopsies. In this review, we present the principles of BEAMing and dPCR as well as the trends of future technical development, focusing on the possibility of developing multiplexed mutation analysis. Finally, we will discuss why such techniques will remain useful despite the ever-decreasing costs and increased automatization of next-generation sequencing (NGS), using molecular characterization of cancer cells as an example.
MeSH term(s)	Biopsy ; DNA Mutational Analysis/methods ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Multiplex Polymerase Chain Reaction/methods ; Neoplasms/genetics ; Polymerase Chain Reaction/methods
Language	English
Publishing date	2017
Publishing country	New Zealand
Document type	Journal Article ; Review
ZDB-ID	2232796-4
ISSN	1179-2000 ; 1177-1062
ISSN (online)	1179-2000
ISSN	1177-1062
DOI	10.1007/s40291-017-0287-7
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