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Article ; Online: Modulation of epileptiform activity by three subgroups of GABAergic interneurons in mouse somatosensory cortex.

Lado, Wudu E / Xu, Xin / Hablitz, John J

2022 Volume 183, Page(s) 106937

Abstract: 4-Aminopyridine (4-AP) induces ictal-like epileptiform discharges in a variety of brain regions. These events are associated with enhanced inhibitory and excitatory synaptic neurotransmission. The relative contribution of specific subclasses of GABAergic ...

Abstract	4-Aminopyridine (4-AP) induces ictal-like epileptiform discharges in a variety of brain regions. These events are associated with enhanced inhibitory and excitatory synaptic neurotransmission. The relative contribution of specific subclasses of GABAergic interneurons (INs) to epileptiform activity in the 4-AP model is not well characterized. We have used genetically encoded channelrhodopsin (ChR) and Archaerhodopsin (Arch) expression in parvalbumin (PV), somatostatin (SST) and vasoactive intestinal polypeptide (VIP) INs to investigate the role of interneuron subclasses in 4-AP-induced epileptiform discharges. Whole-cell patch-clamp recordings were obtained from L5 pyramidal cells (PYRs) in somatosensory cortex of 30-to-70-day old mice. In the presence of 100 µM 4-AP, photostimulation of ChR in PV and SST, but not VIP INs, evoked epileptiform discharges similar to spontaneous and electrically evoked events. Light activation of Arch in PV INs was more effective in reducing epileptiform activity compared to SST and VIP INs. Epileptiform discharges were evoked at offset of Arch induced hyperpolarizations in PV and SST interneurons but not VIP INs. PV and SST INs could both initiate and inhibit 4-AP-induced epileptiform activity in L5 PYRs. VIP INs did not contribute significantly to eliciting or inhibiting epileptiform discharges. These results suggest that subclasses of INs contribute differently to the initiation and modulation of epileptiform discharges in cortical networks.
MeSH term(s)	Action Potentials/physiology ; Animals ; Interneurons/metabolism ; Mice ; Parvalbumins/metabolism ; Pyramidal Cells ; Somatosensory Cortex
Chemical Substances	Parvalbumins
Language	English
Publishing date	2022-04-28
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	632939-1
ISSN	1872-6844 ; 0920-1211
ISSN (online)	1872-6844
ISSN	0920-1211
DOI	10.1016/j.eplepsyres.2022.106937
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Zs.A 2193: Show issues

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Article ; Online: Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition.

Hossein-Javaheri, Nariman / Wilkie, Michael P / Lado, Wudu E / Buck, Leslie T

The Journal of experimental biology

2017 Volume 220, Issue Pt 4, Page(s) 695–704

Abstract: With oxygen deprivation, the mammalian brain undergoes hyper-activity and neuronal death while this does not occur in the anoxia-tolerant goldfish ( ...

Abstract	With oxygen deprivation, the mammalian brain undergoes hyper-activity and neuronal death while this does not occur in the anoxia-tolerant goldfish (
MeSH term(s)	Action Potentials/drug effects ; Anaerobiosis ; Animals ; Fish Proteins/metabolism ; GABA-A Receptor Antagonists/pharmacology ; GABA-B Receptor Antagonists/pharmacology ; Goldfish/physiology ; Humans ; Hypoxia/metabolism ; Oxygen/metabolism ; Patch-Clamp Techniques ; Pyramidal Cells/cytology ; Pyramidal Cells/drug effects ; Pyramidal Cells/metabolism ; Receptors, GABA-A/metabolism ; Receptors, GABA-B/metabolism ; Telencephalon/cytology ; Telencephalon/physiology
Chemical Substances	Fish Proteins ; GABA-A Receptor Antagonists ; GABA-B Receptor Antagonists ; Receptors, GABA-A ; Receptors, GABA-B ; Oxygen (S88TT14065)
Language	English
Publishing date	2017-02-15
Publishing country	England
Document type	Journal Article
ZDB-ID	218085-6
ISSN	1477-9145 ; 0022-0949
ISSN (online)	1477-9145
ISSN	0022-0949
DOI	10.1242/jeb.146605
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Zs.A 2629: Show issues

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Article: Electrophysiological characterization of male goldfish (Carassius auratus) ventral preoptic area neurons receiving olfactory inputs.

Lado, Wudu E / Spanswick, David C / Lewis, John E / Trudeau, Vance L

Frontiers in neuroscience

2014 Volume 8, Page(s) 185

Abstract: Chemical communication via sex pheromones is critical for successful reproduction but the underlying neural mechanisms are not well-understood. The goldfish is a tractable model because sex pheromones have been well-characterized in this species. We used ...

Abstract	Chemical communication via sex pheromones is critical for successful reproduction but the underlying neural mechanisms are not well-understood. The goldfish is a tractable model because sex pheromones have been well-characterized in this species. We used male goldfish forebrain explants in vitro and performed whole-cell current clamp recordings from single neurons in the ventral preoptic area (vPOA) to characterize their membrane properties and synaptic inputs from the olfactory bulbs (OB). Principle component and cluster analyses based on intrinsic membrane properties of vPOA neurons (N = 107) revealed five (I-V) distinct cell groups. These cells displayed differences in their input resistance (Rinput: I < II < IV < III = V), time constant (TC: I = II < IV < III = V), and threshold current (Ithreshold: I > II = IV > III = V). Evidence from electrical stimulation of the OB and application of receptor antagonists suggests that vPOA neurons receive monosynaptic glutamatergic inputs via the medial olfactory tract, with connectivity varying among neuronal groups [I (24%), II (40%), III (0%), IV (34%), and V (2%)].
Language	English
Publishing date	2014-06-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2411902-7
ISSN	1662-453X ; 1662-4548
ISSN (online)	1662-453X
ISSN	1662-4548
DOI	10.3389/fnins.2014.00185
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Article ; Online: Ovulation but not milt production is inhibited in fathead minnows (Pimephales promelas) exposed to a reproductively inhibitory pulp mill effluent.

Waye, Andrew / Lado, Wudu E / Martel, Pierre H / Arnason, John T / Trudeau, Vance L

Reproductive biology and endocrinology : RB&E

2014 Volume 12, Page(s) 43

Abstract: Background: A 5-day fathead minnow (FHM) spawning assay is used by industry to monitor pulp mill effluent quality, with some mill effluents capable of completely inhibiting spawning. The purpose of this report is to characterize the effect of an ... ...

Abstract	Background: A 5-day fathead minnow (FHM) spawning assay is used by industry to monitor pulp mill effluent quality, with some mill effluents capable of completely inhibiting spawning. The purpose of this report is to characterize the effect of an inhibitory effluent on egg and milt production in FHM. Methods: Eight tanks were treated with an inhibitory effluent while eight were kept with clean water. Each tank contained two males and four females as per the 5-day FHM spawning assay used by industry. Females were stripped of ovulated eggs and males of milt in four effluent-exposed and four control tanks. Eggs oviposited in every tank were also counted and checked for fertilization and data analyzed with 2-way ANOVA. Results: We show that female, but not male, fathead minnow reproductive function is impaired in the 5-day fathead minnow spawning assay used by industry to evaluate pulp mill effluent quality in Canada. Milt production was not changed in the control or exposed males mid-way and at the end of the five day exposure (p > 0.05; n = 8). Total egg production (stripped + oviposited) was impaired (p < 0.05) in fathead minnows exposed to effluent (288 eggs/tank, n = 4 tanks) compared to those in control tanks (753 eggs/tank, n = 4 tanks). Conclusions: Our results indicate that males are able to detect female signals and prepare appropriately for spawning while in females inhibition of ovulation is occurring somewhere along the hypothalamus-pituitary-gonad reproductive axis. These results suggest female-specific neuroendocrine disruption and provide mechanistic insight into an assay used by industry to assess pulp mill effluent quality.
MeSH term(s)	Animals ; Aquaculture ; Biological Assay ; Canada ; Cyprinidae/physiology ; Drug Resistance ; Endocrine Disruptors/analysis ; Endocrine Disruptors/toxicity ; Female ; Fertilization/drug effects ; Infertility, Female/chemically induced ; Infertility, Male/chemically induced ; Male ; Oviposition/drug effects ; Ovulation/drug effects ; Ovum/drug effects ; Sex Characteristics ; Waste Water/chemistry ; Waste Water/toxicity ; Water Pollutants, Chemical/analysis ; Water Pollutants, Chemical/toxicity ; Water Quality ; Wood
Chemical Substances	Endocrine Disruptors ; Waste Water ; Water Pollutants, Chemical
Language	English
Publishing date	2014-05-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1477-7827
ISSN (online)	1477-7827
DOI	10.1186/1477-7827-12-43
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Article: Increased conditioned immobility and weight loss in rats following mechanical impacts to the skull that do not produce loss of consciousness

Lado, Wudu E / Persinger, Michael A

Central European journal of biology. 2008 Dec., v. 3, no. 4

2008

Abstract: Rats either received a single vertical impact (15 km/h) of mechanical energy to their right dorsal skulls over the parietal region or served as handled controls. About 50% of the rats appeared normal after the impact. Thirty days later there were ... ...

Abstract	Rats either received a single vertical impact (15 km/h) of mechanical energy to their right dorsal skulls over the parietal region or served as handled controls. About 50% of the rats appeared normal after the impact. Thirty days later there were conspicuous areas containing neurons with shrunken and darkly stained somas within the cortices beneath the impact site and within the amygdala and entorhinal cortices. These neurons, occupying an average total area that ranged from 0.50 mm² to 5 mm², were evident even in rats that showed no stunning following the impact. These neurons were not seen in control rats. Subsequent decreases in body weight for rats that received the impact (even with no obvious stunning) were attenuated by oral access to 10% glucose but not by treatment with acetaminophen or ketamine. The rats that sustained the impact also displayed increased immobility within settings with which an aversive stimulus had been associated. Post-impact injection with ketamine did not normalize this behaviour. These results show that quantitative changes in some neuronal soma remain weeks after a single impact of mechanical energy that is not associated with immediate changes in behaviour. Concomitant with these neuronal alterations was increased emotional responsiveness to contexts associated with a single aversive episode and transient decreases in body weights.
Keywords	weight loss
Language	English
Dates of publication	2008-12
Size	p. 422-430.
Publisher	SP Versita
Publishing place	Heidelberg
Document type	Article
ISSN	1644-3632
DOI	10.2478/s11535-008-0041-6
Database	NAL-Catalogue (AGRICOLA)

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Article: Rapid modulation of gene expression profiles in the telencephalon of male goldfish following exposure to waterborne sex pheromones

Lado, Wudu E / Zhang, Dapeng / Mennigen, Jan A / Zamora, Jacob M / Popesku, Jason T / Trudeau, Vance L

General and Comparative Endocrinology. 2013 Oct. 1, v. 192

2013

Abstract: Sex pheromones rapidly affect endocrine physiology and behaviour, but little is known about their effects on gene expression in the neural tissues that mediate olfactory processing. In this study, we exposed male goldfish for 6h to waterborne 17,20βP (4 ... ...

Abstract	Sex pheromones rapidly affect endocrine physiology and behaviour, but little is known about their effects on gene expression in the neural tissues that mediate olfactory processing. In this study, we exposed male goldfish for 6h to waterborne 17,20βP (4.3nM) and PGF₂α (3nM), the main pre-ovulatory and post-ovulatory pheromones, respectively. Both treatments elevated milt volume (P=0.001). Microarray analysis of male telencephalon following PGF₂α treatment identified 71 unique transcripts that were differentially expressed (q<5%; 67 up, 4 down). Functional annotation of these regulated genes indicates that PGF₂α pheromone exposure affects diverse biological processes including nervous system functions, energy metabolism, cholesterol/lipoprotein transport, translational regulation, transcription and chromatin remodelling, protein processing, cytoskeletal organization, and signalling. By using real-time RT-PCR, we further validated three candidate genes, ependymin-II, calmodulin-A and aldolase C, which exhibited 3–5-fold increase in expression following PGF₂α exposure. Expression levels of some other genes that are thought to be important for reproduction were also determined using real-time RT-PCR. Expression of sGnRH was increased by PGF₂α, but not 17,20βP, whereas cGnRH expression was increased by 17,20βP but not PGF₂α. In contrast, both pheromones increase the expression of glutamate (GluR2a, NR2A) and γ-aminobutyric acid (GABAA γ2) receptor subunit mRNAs. Milt release and rapid modulation of neuronal transcription are part of the response of males to female sex pheromones.
Keywords	cholesterol ; chromatin ; cytoskeleton ; energy metabolism ; fructose-bisphosphate aldolase ; gamma-aminobutyric acid ; gene expression ; gene expression regulation ; genes ; glutamic acid ; goldfish ; gonadotropin-releasing hormone ; lipoproteins ; males ; messenger RNA ; microarray technology ; milt ; nervous system ; reproduction ; reverse transcriptase polymerase chain reaction ; sex pheromones
Language	English
Dates of publication	2013-1001
Size	p. 204-213.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	1851-x
ISSN	1095-6840 ; 0016-6480
ISSN (online)	1095-6840
ISSN	0016-6480
DOI	10.1016/j.ygcen.2013.06.015
Database	NAL-Catalogue (AGRICOLA)

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Z 88/702: Show issues

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Article ; Online: Rapid modulation of gene expression profiles in the telencephalon of male goldfish following exposure to waterborne sex pheromones.

Lado, Wudu E / Zhang, Dapeng / Mennigen, Jan A / Zamora, Jacob M / Popesku, Jason T / Trudeau, Vance L

General and comparative endocrinology

2013 Volume 192, Page(s) 204–213

Abstract	Sex pheromones rapidly affect endocrine physiology and behaviour, but little is known about their effects on gene expression in the neural tissues that mediate olfactory processing. In this study, we exposed male goldfish for 6h to waterborne 17,20βP (4.3 nM) and PGF2α (3 nM), the main pre-ovulatory and post-ovulatory pheromones, respectively. Both treatments elevated milt volume (P=0.001). Microarray analysis of male telencephalon following PGF2α treatment identified 71 unique transcripts that were differentially expressed (q<5%; 67 up, 4 down). Functional annotation of these regulated genes indicates that PGF2α pheromone exposure affects diverse biological processes including nervous system functions, energy metabolism, cholesterol/lipoprotein transport, translational regulation, transcription and chromatin remodelling, protein processing, cytoskeletal organization, and signalling. By using real-time RT-PCR, we further validated three candidate genes, ependymin-II, calmodulin-A and aldolase C, which exhibited 3-5-fold increase in expression following PGF2α exposure. Expression levels of some other genes that are thought to be important for reproduction were also determined using real-time RT-PCR. Expression of sGnRH was increased by PGF2α, but not 17,20βP, whereas cGnRH expression was increased by 17,20βP but not PGF2α. In contrast, both pheromones increase the expression of glutamate (GluR2a, NR2A) and γ-aminobutyric acid (GABAA γ2) receptor subunit mRNAs. Milt release and rapid modulation of neuronal transcription are part of the response of males to female sex pheromones.
MeSH term(s)	Animals ; Dinoprost/pharmacology ; Female ; Goldfish/metabolism ; Male ; Sex Attractants/pharmacology ; Telencephalon/drug effects ; Telencephalon/metabolism
Chemical Substances	Sex Attractants ; Dinoprost (B7IN85G1HY)
Language	English
Publishing date	2013-10-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1851-x
ISSN	1095-6840 ; 0016-6480
ISSN (online)	1095-6840
ISSN	0016-6480
DOI	10.1016/j.ygcen.2013.06.015
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Article ; Online: Estrogen-like effects in male goldfish co-exposed to fluoxetine and 17 alpha-ethinylestradiol.

Silva de Assis, Helena C / Simmons, Denina B D / Zamora, Jacob M / Lado, Wudu E / Al-Ansari, Ahmed M / Sherry, James P / Blais, Jules M / Metcalfe, Chris D / Trudeau, Vance L

Environmental science & technology

2013 Volume 47, Issue 10, Page(s) 5372–5382

Abstract: The antidepressant fluoxetine (FLX) and the synthetic estrogen, 17 alpha-ethinylestradiol (EE2), are present in municipal sewage discharges. To better understand possible interactions between them, male goldfish were exposed to an ethanol control or to ... ...

Abstract	The antidepressant fluoxetine (FLX) and the synthetic estrogen, 17 alpha-ethinylestradiol (EE2), are present in municipal sewage discharges. To better understand possible interactions between them, male goldfish were exposed to an ethanol control or to nominal concentrations of FLX (0.54 μg/L) and EE2 (5 ng/L) alone and in combination for 14 days. Real-time reverse-transcription polymerase chain reaction was used to assess effects on hepatic gene expression and liquid chromatography tandem mass spectrometry to analyze the plasma proteome. The results showed an increase in estrogen receptor alpha (esr1) and vitellogenin (vtg) gene expression by 1.9-2.4-fold in the FLX and EE2 groups, but this did not reach statistical significance. In contrast, co-exposure up regulated esr1 and vtg gene expression by 5.5- and 5.3-fold, respectively. Fluoxetine and EE2 alone did not affect estrogen receptor beta (esr2), but the co-exposure down regulated esr2 expression by 50%. There was a significant increase in the number of plasma proteins that were related to endocrine system disorders in the FLX and FLX plus EE2 groups. The level of VTG protein was increased in the plasma from goldfish exposed to EE2, FLX, and FLX plus EE2. Our study demonstrates that low concentrations of FLX and EE2 in a simple mixture produce strong estrogen-like effects in the male goldfish.
MeSH term(s)	Animals ; Base Sequence ; Chromatography, Liquid ; DNA Primers ; Estrogens/analysis ; Estrogens/pharmacology ; Ethinyl Estradiol/pharmacology ; Fluoxetine/analysis ; Fluoxetine/pharmacology ; Gas Chromatography-Mass Spectrometry ; Goldfish ; Male ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Serotonin Uptake Inhibitors/analysis ; Serotonin Uptake Inhibitors/pharmacology ; Tandem Mass Spectrometry ; Water Pollutants, Chemical/analysis ; Water Pollutants, Chemical/pharmacology
Chemical Substances	DNA Primers ; Estrogens ; Serotonin Uptake Inhibitors ; Water Pollutants, Chemical ; Fluoxetine (01K63SUP8D) ; Ethinyl Estradiol (423D2T571U)
Language	English
Publishing date	2013-05-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/es3044888
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Article: Estrogen-like Effects in Male Goldfish Co-exposed to Fluoxetine and 17 Alpha-Ethinylestradiol

Silva de Assis, Helena C / Al-Ansari Ahmed M / Blais Jules M / Lado Wudu E / Metcalfe Chris D / Sherry James P / Simmons Denina B. D / Trudeau Vance L / Zamora Jacob M

Environmental Science & Technology. 2013 May 21, v. 47, no. 10

2013

Abstract	The antidepressant fluoxetine (FLX) and the synthetic estrogen, 17 alpha-ethinylestradiol (EE2), are present in municipal sewage discharges. To better understand possible interactions between them, male goldfish were exposed to an ethanol control or to nominal concentrations of FLX (0.54 Î¼g/L) and EE2 (5 ng/L) alone and in combination for 14 days. Real-time reverse-transcription polymerase chain reaction was used to assess effects on hepatic gene expression and liquid chromatography tandem mass spectrometry to analyze the plasma proteome. The results showed an increase in estrogen receptor alpha (esr1) and vitellogenin (vtg) gene expression by 1.9â2.4-fold in the FLX and EE2 groups, but this did not reach statistical significance. In contrast, co-exposure up regulated esr1 and vtg gene expression by 5.5- and 5.3-fold, respectively. Fluoxetine and EE2 alone did not affect estrogen receptor beta (esr2), but the co-exposure down regulated esr2 expression by 50%. There was a significant increase in the number of plasma proteins that were related to endocrine system disorders in the FLX and FLX plus EE2 groups. The level of VTG protein was increased in the plasma from goldfish exposed to EE2, FLX, and FLX plus EE2. Our study demonstrates that low concentrations of FLX and EE2 in a simple mixture produce strong estrogen-like effects in the male goldfish.
Keywords	antidepressants ; blood proteins ; endocrine system ; estrogen receptors ; ethanol ; gene expression ; gene expression regulation ; goldfish ; liquid chromatography ; males ; proteome ; reverse transcriptase polymerase chain reaction ; sewage ; synthetic estrogens ; tandem mass spectrometry ; transcription (genetics) ; vitellogenin
Language	English
Dates of publication	2013-0521
Size	p. 5372-5382.
Publishing place	American Chemical Society
Document type	Article
ISSN	1520-5851
DOI	10.1021%2Fes3044888
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Waterborne fluoxetine disrupts the reproductive axis in sexually mature male goldfish, Carassius auratus.

Mennigen, Jan A / Lado, Wudu E / Zamora, Jake M / Duarte-Guterman, Paula / Langlois, Valérie S / Metcalfe, Chris D / Chang, John P / Moon, Thomas W / Trudeau, Vance L

Aquatic toxicology (Amsterdam, Netherlands)

2010 Volume 100, Issue 4, Page(s) 354–364

Abstract: Fluoxetine (FLX) is a pharmaceutical acting as a selective serotonin reuptake inhibitor and is used to treat depression in humans. Fluoxetine and the major active metabolite norfluoxetine (NFLX) are released to aquatic systems via sewage-treatment ... ...

Abstract	Fluoxetine (FLX) is a pharmaceutical acting as a selective serotonin reuptake inhibitor and is used to treat depression in humans. Fluoxetine and the major active metabolite norfluoxetine (NFLX) are released to aquatic systems via sewage-treatment effluents. They have been found to bioconcentrate in wild fish, raising concerns over potential endocrine disrupting effects. The objective of this study was to determine effects of waterborne FLX, including environmental concentrations, on the reproductive axis in sexually mature male goldfish. We initially cloned the goldfish serotonin transporter to investigate tissue and temporal expression of the serotonin transporter, the FLX target, in order to determine target tissues and sensitive exposure windows. Sexually mature male goldfish, which showed the highest levels of serotonin transporter expression in the neuroendocrine brain, were exposed to FLX at 0.54μg/L and 54μg/L in a 14-d exposure before receiving vehicle or sex pheromone stimulus consisting of either 4.3nM 17,20β-dihydroxy-4-pregnene-3-one (17,20P) or 3nM prostaglandin F₂(α) (PGF₂(α)). Reproductive endpoints assessed included gonadosomatic index, milt volume, and blood levels of the sex steroids testosterone and estradiol. Neuroendocrine function was investigated by measuring blood levels of luteinizing hormone, growth hormone, pituitary gene expression of luteinizing hormone, growth hormone and follicle-stimulating hormone and neuroendocrine brain expression of isotocin and vasotocin. To investigate changes at the gonadal level of the reproductive axis, testicular gene expression of the gonadotropin receptors, both the luteinizing hormone receptor and the follicle-stimulating hormone receptor, were measured as well as expression of the growth hormone receptor. To investigate potential impacts on spermatogenesis, testicular gene expression of the spermatogenesis marker vasa was measured and histological samples of testis were analyzed qualitatively. Estrogen indices were measured by expression and activity analysis of gonadal aromatase, as well as liver expression analysis of the estrogenic marker, esr1. After 14d, basal milt volume significantly decreased at 54μg/L FLX while pheromone-stimulated milt volume decreased at 0.54μg/L and 54μg/L FLX. Fluoxetine (54μg/L) inhibited both basal and pheromone-stimulated testosterone levels. Significant concentration-dependent reductions in follicle-stimulating hormone and isotocin expression were observed with FLX in the 17,20P- and PGF₂(α)-stimulated groups, respectively. Estradiol levels and expression of esr1 concentration-dependently increased with FLX. This study demonstrates that FLX disrupts reproductive physiology of male fish at environmentally relevant concentrations, and potential mechanisms are discussed.
MeSH term(s)	Animals ; Aromatase/metabolism ; Base Sequence ; Estrogen Receptor alpha/metabolism ; Fish Proteins/genetics ; Fish Proteins/metabolism ; Fluoxetine/toxicity ; Gene Expression/drug effects ; Goldfish/genetics ; Goldfish/physiology ; Liver/metabolism ; Male ; Molecular Sequence Data ; Oxytocin/analogs & derivatives ; Oxytocin/genetics ; Oxytocin/metabolism ; Prostaglandins F/genetics ; Prostaglandins F/metabolism ; Reproduction/drug effects ; Serotonin Plasma Membrane Transport Proteins/drug effects ; Serotonin Uptake Inhibitors/toxicity ; Testis/metabolism ; Testosterone/metabolism ; Water Pollutants, Chemical/toxicity
Chemical Substances	Estrogen Receptor alpha ; Fish Proteins ; Prostaglandins F ; Serotonin Plasma Membrane Transport Proteins ; Serotonin Uptake Inhibitors ; Water Pollutants, Chemical ; Fluoxetine (01K63SUP8D) ; Testosterone (3XMK78S47O) ; Oxytocin (50-56-6) ; isotocin (550-21-0) ; prostaglandin F3alpha (745-64-2) ; Aromatase (EC 1.14.14.1)
Language	English
Publishing date	2010-11-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	782699-0
ISSN	1879-1514 ; 0166-445X
ISSN (online)	1879-1514
ISSN	0166-445X
DOI	10.1016/j.aquatox.2010.08.016
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