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Article ; Online: An Integrated Affinity Chromatography-Based Approach to Unravel the sRNA Interactome in Nitrogen-Fixing Rhizobia.

García-Tomsig, Natalia Isabel / Lagares, Antonio / Becker, Anke / Valverde, Claudio / Jiménez-Zurdo, José Ignacio

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2741, Page(s) 363–380

Abstract: The activity mechanism and function of bacterial base-pairing small non-coding RNA regulators (sRNAs) are largely shaped by their main interacting cellular partners, i.e., proteins and mRNAs. We describe here an MS2 affinity chromatography-based ... ...

Abstract	The activity mechanism and function of bacterial base-pairing small non-coding RNA regulators (sRNAs) are largely shaped by their main interacting cellular partners, i.e., proteins and mRNAs. We describe here an MS2 affinity chromatography-based procedure adapted to unravel the sRNA interactome in nitrogen-fixing legume endosymbiotic bacteria. The method consists of tagging of the bait sRNA at its 5'-end with the MS2 aptamer followed by pulse overexpression and immobilization of the chimeric transcript from cell lysates by an MS2-MBP fusion protein conjugated to an amylose resin. The sRNA-binding proteins and target mRNAs are further profiled by mass spectrometry and RNAseq, respectively.
MeSH term(s)	RNA, Small Untranslated/genetics ; Rhizobium/genetics ; Rhizobium/metabolism ; Nitrogen/metabolism ; Bacteria/genetics ; Nitrogen-Fixing Bacteria/genetics ; Chromatography, Affinity/methods ; RNA, Bacterial/genetics ; Gene Expression Regulation, Bacterial
Chemical Substances	RNA, Small Untranslated ; Nitrogen (N762921K75) ; RNA, Bacterial
Language	English
Publishing date	2024-01-13
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3565-0_19
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Article: Pericytes and vascular smooth muscle cells in central nervous system arteriovenous malformations.

Nakisli, Sera / Lagares, Alfonso / Nielsen, Corinne M / Cuervo, Henar

Frontiers in physiology

2023 Volume 14, Page(s) 1210563

Abstract: Previously considered passive support cells, mural cells-pericytes and vascular smooth muscle cells-have started to garner more attention in disease research, as more subclassifications, based on morphology, gene expression, and function, have been ... ...

Abstract	Previously considered passive support cells, mural cells-pericytes and vascular smooth muscle cells-have started to garner more attention in disease research, as more subclassifications, based on morphology, gene expression, and function, have been discovered. Central nervous system (CNS) arteriovenous malformations (AVMs) represent a neurovascular disorder in which mural cells have been shown to be affected, both in animal models and in human patients. To study consequences to mural cells in the context of AVMs, various animal models have been developed to mimic and predict human AVM pathologies. A key takeaway from recently published work is that AVMs and mural cells are heterogeneous in their molecular, cellular, and functional characteristics. In this review, we summarize the observed perturbations to mural cells in human CNS AVM samples and CNS AVM animal models, and we discuss various potential mechanisms relating mural cell pathologies to AVMs.
Language	English
Publishing date	2023-08-04
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2023.1210563
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Article ; Online: Analysis of hospital costs by morbidity group for patients with severe mental illness.

Caballer-Tarazona, Vicent / Zúñiga-Lagares, Antonio / Reyes-Santias, Francisco

Annals of medicine

2022 Volume 54, Issue 1, Page(s) 858–866

Abstract: Objectives: The goal of this study is to analyse hospital costs and length of stay of patients admitted to psychiatric units in hospitals in a European region of the Mediterranean Arc. The aim is to identify the effects of comorbidities and other ... ...

Abstract	Objectives: The goal of this study is to analyse hospital costs and length of stay of patients admitted to psychiatric units in hospitals in a European region of the Mediterranean Arc. The aim is to identify the effects of comorbidities and other variables in order to create an explanatory cost model. Methods: In order to carry out the study, the Ministry of Health was asked to provide data on access to the mental health facilities of all hospitals in the region. Among other questions, this database identifies the most important diagnostic variables related to admission, like comorbidities, age and gender. The method used, based on the Manning-Mullahy algorithm, was linear regression. The results were measured by the statistical significance of the independent variables to determine which of them were valid to explain the cost of hospitalization. Results: Psychiatric inpatients can be divided into three main groups (psychotic, organic and neurotic), which have statistically significant differences in costs. The independent variables that were statistically significant ( Conclusions: Unlike most health services, access to psychiatric facilities does not correlate with comorbidities due to the specific nature of this specialization. Patients admitted to psychosis had higher costs and a higher number of average staysKEY MESSAGESThe highest average hospital expenditure occurred in patients admitted for psychotic disorders.Due to the particularities of psychiatry units and unlike other medical specialties, the number of comorbidities did not influence the number of hospital stays or hospital expenditure.Apart from the main diagnostic group, the variables that were useful to explain hospital expenditure were the presence of poisoning and injuries as comorbidity, diseases of circulatory system as comorbidity, history of substance abuse and readmission.
MeSH term(s)	Hospital Costs ; Hospitals ; Humans ; Length of Stay ; Mental Disorders/epidemiology ; Morbidity ; Substance-Related Disorders
Language	English
Publishing date	2022-03-23
Publishing country	England
Document type	Journal Article
ZDB-ID	1004226-x
ISSN	1365-2060 ; 1651-2219 ; 0785-3890 ; 1743-1387
ISSN (online)	1365-2060 ; 1651-2219
ISSN	0785-3890 ; 1743-1387
DOI	10.1080/07853890.2022.2048884
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Article ; Online: In Reply: Precautions for Endoscopic Transnasal Skull Base Surgery During the COVID-19 Pandemic.

Paredes, Igor / García, Alfredo / Garcia, Esther / Lagares, Alfonso

Neurosurgery

2021 Volume 89, Issue 2, Page(s) E133–E134

MeSH term(s)	COVID-19 ; Endoscopy ; Humans ; Pandemics ; SARS-CoV-2 ; Skull Base/surgery
Language	English
Publishing date	2021-04-28
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	135446-2
ISSN	1524-4040 ; 0148-396X
ISSN (online)	1524-4040
ISSN	0148-396X
DOI	10.1093/neuros/nyab160
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Zs.A 1424: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Biomarkers for Pre-Treatment Risk Stratification of Prostate Cancer Patients: A Systematic Review.

Sequeira, José Pedro / Salta, Sofia / Freitas, Rui / López-López, Rafael / Díaz-Lagares, Ángel / Henrique, Rui / Jerónimo, Carmen

Cancers

2024 Volume 16, Issue 7

Abstract: Background: Prostate cancer (PCa) is one of the most frequently occurring malignancies. Although most cases are not life-threatening, approximately 20% endure an unfavorable outcome. PSA-based screening reduced mortality but at the cost of an increased ... ...

Abstract	Background: Prostate cancer (PCa) is one of the most frequently occurring malignancies. Although most cases are not life-threatening, approximately 20% endure an unfavorable outcome. PSA-based screening reduced mortality but at the cost of an increased overdiagnosis/overtreatment of low-risk (lrPCa) and favorable intermediate-risk (firPCa) PCa. PCa risk-groups are usually identified based on serum Prostate-Specific Antigen (PSA), the Gleason score, and clinical T stage, which have consistent although variable specificity or subjectivity. Thus, more effective and specific tools for risk assessment are needed, ideally making use of minimally invasive methods such as liquid biopsies. In this systematic review we assessed the clinical potential and analytical performance of liquid biopsy-based biomarkers for pre-treatment risk stratification of PCa patients. Methods: Studies that assessed PCa pre-treatment risk were retrieved from PubMed, Scopus, and MedLine. PCa risk biomarkers were analyzed, and the studies' quality was assessed using the QUADAS-2 tool. Results: The final analysis comprised 24 full-text articles, in which case-control studies predominated, mostly reporting urine-based biomarkers (54.2%) and biomarker quantification by qPCR (41.7%). Categorization into risk groups was heterogeneous, predominantly making use of the Gleason score. Conclusion: This systematic review unveils the substantial clinical promise of using circulating biomarkers in assessing the risk for prostate cancer patients. However, the standardization of groups, categories, and biomarker validation are mandatory before this technique can be implemented. Circulating biomarkers might represent a viable alternative to currently available tools, obviating the need for tissue biopsies, and allowing for faster and more cost-effective testing, with superior analytical performance, specificity, and reproducibility.
Language	English
Publishing date	2024-03-30
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers16071363
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Article ; Online: Twelve controversial questions in aneurysmal subarachnoid hemorrhage.

Llompart-Pou, Juan Antonio / Pérez-Bárcena, Jon / Lagares, Alfonso / Godoy, Daniel Agustín

Medicina intensiva

2023 Volume 48, Issue 2, Page(s) 92–102

Abstract: Critical care management of aneurysmal subarachnoid hemorrhage (aSAH) remains a major challenge. Despite the recent publication of guidelines from the American Heart Association/American Stroke Association and the Neurocritical Care Society, there are ... ...

Abstract	Critical care management of aneurysmal subarachnoid hemorrhage (aSAH) remains a major challenge. Despite the recent publication of guidelines from the American Heart Association/American Stroke Association and the Neurocritical Care Society, there are many controversial questions in the intensive care unit (ICU) management of this population. The authors provide an analysis of common issues in the ICU and provide guidance on the daily management of this specific population of neurocritical care patients.
MeSH term(s)	United States ; Humans ; Subarachnoid Hemorrhage/complications ; Subarachnoid Hemorrhage/therapy ; Stroke ; Critical Care ; Intensive Care Units
Language	English
Publishing date	2023-11-10
Publishing country	Spain
Document type	Journal Article ; Review
ISSN	2173-5727
ISSN (online)	2173-5727
DOI	10.1016/j.medine.2023.09.003
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Article ; Online: Atlanto-occipital dislocation with concomitant severe traumatic brain injury: A retrospective study at a level 1 trauma center.

García-Pérez, Daniel / Panero, Irene / Lagares, Alfonso / Gómez, Pedro Antonio / Alén, José F / Paredes, Igor

Neurocirugia (English Edition)

2023 Volume 34, Issue 1, Page(s) 12–21

Abstract: Background: Traumatic atlanto-occipital dislocation (AOD) is a life-threatening injury. Although traumatic brain injury (TBI) is associated with increased mortality in AOD patients, a detailed individual analysis of these patients is lacking in the ... ...

Abstract	Background: Traumatic atlanto-occipital dislocation (AOD) is a life-threatening injury. Although traumatic brain injury (TBI) is associated with increased mortality in AOD patients, a detailed individual analysis of these patients is lacking in the literature. Methods: Patients ≥16 years old who were diagnosed of AOD with concomitant severe TBI from 2010 to 2020 were included in this retrospective study. We examined the epidemiology, injury mechanisms, associated injuries, and outcomes of these patients. Results: Eight patients were included. Six patients died before any intervention could be performed, and two patients underwent an occipito-cervical fixation, showing a notorious neurologic improvement on follow-up. Cardiorespiratory arrest (CRA) was a strong predictor of subsequent death. CT signs of diffuse axonal injury (DAI) were present in most patients and were confirmed by magnetic resonance imaging (MRI) in survivors. Although TBI was not the main cause of death, it was responsible for the delayed neurological improvement and deferred stabilization. The average sensitivity of the different used methodologies for AOD diagnosis ranged from 0.50 to 1.00, being the Basion Dens Interval (BDI) and the Condyle-C1 interval (CCI) sum the most reliable criteria. Non-survivors tended to show greater distraction measurements. The high incidence of condylar avulsion fractures suggests that their visualization on the initial CT study should heighten the suspicion for AOD. Conclusions: Our data suggest that patients with AOD and concomitant severe TBI might be salvageable patients. In those who survive beyond the first hospital days and show neurological improvement, surgical treatment should be performed as they can achieve an important neurologic recovery.
MeSH term(s)	Humans ; Adolescent ; Retrospective Studies ; Trauma Centers ; Atlanto-Occipital Joint/diagnostic imaging ; Atlanto-Occipital Joint/injuries ; Tomography, X-Ray Computed/methods ; Joint Dislocations/diagnostic imaging ; Joint Dislocations/epidemiology ; Joint Dislocations/etiology ; Brain Injuries, Traumatic/complications ; Brain Injuries, Traumatic/diagnostic imaging ; Brain Injuries, Traumatic/epidemiology
Language	English
Publishing date	2023-01-07
Publishing country	Spain
Document type	Journal Article
ISSN	2529-8496
ISSN (online)	2529-8496
DOI	10.1016/j.neucie.2022.11.004
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Article ; Online: Low rCBV values in glioblastoma tumor progression under chemoradiotherapy.

Hilario, A / Salvador, E / Cardenas, A / Romero, J / Lechuga, C / Chen, Z / Martinez de Aragon, A / Perez-Nuñez, A / Hernandez-Lain, A / Sepulveda, J / Lagares, A / Toldos, O / Rodriguez-Gonzalez, V / Ramos, A

Neuroradiology

2024 Volume 66, Issue 3, Page(s) 317–323

Abstract: Purpose: After standard treatment for glioblastoma, perfusion MRI remains challenging for differentiating tumor progression from post-treatment changes. Our objectives were (1) to correlate rCBV values at diagnosis and at first tumor progression and (2) ...

Abstract	Purpose: After standard treatment for glioblastoma, perfusion MRI remains challenging for differentiating tumor progression from post-treatment changes. Our objectives were (1) to correlate rCBV values at diagnosis and at first tumor progression and (2) to analyze the relationship of rCBV values at tumor recurrence with enhancing volume, localization of tumor progression, and time elapsed since the end of radiotherapy in tumor recurrence. Methods: Inclusion criteria were (1) age > 18 years, (2) histologically confirmed glioblastoma treated with STUPP regimen, and (3) tumor progression according to RANO criteria > 12 weeks after radiotherapy. Co-registration of segmented enhancing tumor VOIs with dynamic susceptibility contrast perfusion MRI was performed using Olea Sphere software. For tumor recurrence, we correlated rCBV values with enhancing tumor volume, with recurrence localization, and with time elapsed from the end of radiotherapy to progression. Analyses were performed with SPSS software. Results: Sixty-four patients with glioblastoma were included in the study. Changes in rCBV values between diagnosis and first tumor progression were significant (p < 0.001), with a mean and median decreases of 32% and 46%, respectively. Mean rCBV values were also different (p < 0.01) when tumors progressed distally (radiation field rCBV values of 1.679 versus 3.409 distally). However, changes and, therefore, low rCBV values after radiotherapy in tumor recurrence were independent of time. Conclusion: Chemoradiation alters tumor perfusion and rCBV values may be decreased in the setting of tumor progression. Changes in rCBV values with respect to diagnosis, with low rCBV in tumor progression, are independent of time but related to the site of recurrence.
MeSH term(s)	Humans ; Adult ; Middle Aged ; Glioblastoma/diagnostic imaging ; Glioblastoma/radiotherapy ; Brain Neoplasms/diagnostic imaging ; Brain Neoplasms/radiotherapy ; Neoplasm Recurrence, Local/diagnostic imaging ; Contrast Media ; Chemoradiotherapy ; Magnetic Resonance Imaging/methods
Chemical Substances	Contrast Media
Language	English
Publishing date	2024-01-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	123305-1
ISSN	1432-1920 ; 0028-3940
ISSN (online)	1432-1920
ISSN	0028-3940
DOI	10.1007/s00234-023-03279-7
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Article ; Online: Guidelines for Inferring and Characterizing a Family of Bacterial trans-Acting Small Noncoding RNAs.

Lagares, Antonio / Valverde, Claudio

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1737, Page(s) 31–45

Abstract: So far, every sequenced bacterial transcriptome encompasses hundreds of small regulatory noncoding RNAs (sRNAs). From those sRNAs that have been already characterized, we learned that their regulatory functions could span over almost every bacterial ... ...

Abstract	So far, every sequenced bacterial transcriptome encompasses hundreds of small regulatory noncoding RNAs (sRNAs). From those sRNAs that have been already characterized, we learned that their regulatory functions could span over almost every bacterial process, mostly acting at the posttranscriptional control of gene expression (Wagner and Romby, Adv Genet 90:133-208, 2015). Canonical molecular mechanisms of sRNA action have been described to rely on both sequence and/or structural traits of the RNA molecule. As for protein-coding genes, the conservation of sRNAs among species suggests conserved and adjusted functions across evolution. Knowing the phylogenetic distribution of an sRNA gene and how its functional traits have evolved may help to get a broad picture of its biological role in each single species. Here, we present a simple computational workflow to identify close and distant sRNA homologs present in sequenced bacterial genomes, which allows defining novel sRNA families. This strategy is based on the use of Covariance Models (CM) and assumes the conservation of sequence and structure of functional sRNA genes throughout evolution. Moreover, by carefully inspecting the conservation of the close genomic context of every member of the RNA family and how the patterns of microsynteny follow the path of species evolution, it is possible to define subgroups of sRNA orthologs, which in turn enables the definition of RNA subfamilies.
MeSH term(s)	Bacteria/genetics ; Computational Biology/methods ; Evolution, Molecular ; Gene Expression Regulation, Bacterial ; Genome, Bacterial ; Guidelines as Topic ; Molecular Sequence Annotation ; Phylogeny ; RNA, Bacterial/genetics ; RNA, Small Untranslated/genetics ; Sequence Analysis, RNA/methods ; Trans-Activators/genetics
Chemical Substances	RNA, Bacterial ; RNA, Small Untranslated ; Trans-Activators
Language	English
Publishing date	2018-02-26
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-7634-8_2
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Article: Opening of unusual vascular collaterals leads to early recanalization of a giant intracavernous carotid artery aneurysm following common carotid artery occlusion: A Case report and literature review.

Castaño-Leon, Ana M / Alen, Jose F / Lagares, Alfonso

Surgical neurology international

2020 Volume 11, Page(s) 62

Abstract: Background: Parent artery occlusion (PAO) with or without bypass surgery is a feasible treatment for large intracavernous carotid artery (ICCA) aneurysms. The ideal occlusion site (internal or common carotid artery [CCA]) and ischemic complications ... ...

Abstract	Background: Parent artery occlusion (PAO) with or without bypass surgery is a feasible treatment for large intracavernous carotid artery (ICCA) aneurysms. The ideal occlusion site (internal or common carotid artery [CCA]) and ischemic complications after PAO have received special attention since the description of the technique. Unfrequently, some patients can also develop unusual external carotid artery-internal carotid artery collateral pathways distal to the ligation site that can explain the failure to aneurysm size reduction. Case description: We describe a rare case of delayed refilling of a large ICCA aneurysm partially thrombosed which early recanalized after surgical ligation of the cervical CCA through an unusual collateral pathway. Conclusion: Based on our experience, we recommend periodic long-term follow-up neuroimaging, especially in those cases where potential collateral branches have not been clearly identified in the preoperative studies.
Language	English
Publishing date	2020-04-04
Publishing country	United States
Document type	Case Reports
ISSN	2229-5097
ISSN	2229-5097
DOI	10.25259/SNI_597_2019
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