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Article ; Online: The quality and reliability of patient education regarding sound therapy videos for tinnitus on YouTube.

Huang, Chao / Lan, Hongli / Jiang, Fan / Huang, Yu / Lai, Dan

2024 Volume 12, Page(s) e16846

Abstract: Background: Numerous online videos are available on sound therapy as a treatment modality for tinnitus, but it is uncertain if these videos are adequate for patient education. This study aims to evaluate the quality and reliability of tinnitus sound ... ...

Abstract	Background: Numerous online videos are available on sound therapy as a treatment modality for tinnitus, but it is uncertain if these videos are adequate for patient education. This study aims to evaluate the quality and reliability of tinnitus sound therapy videos on YouTube for patient education. Methods: YouTube videos were searched using keywords related to "tinnitus sound therapy". The top 100 videos were analyzed after excluding those were repetitive, irrelevant, less than 3 min, or not in English. After categorising the videos based on their authorship and content, the video power index (VPI) was relied to determine their popularity. The DISCERN questionnaire (DISCERN), the Global Quality Score (GQS), the Journal of the American Medical Association benchmark criteria (JAMA), and the Patient Education Materials Assessment Tool (PEMAT) were utilized to evaluate the quality, transparency, and patient education. Results: Over half (56%) of the videos were published by professional organizations. A total of 93% of them contained sound only. Only 17% followed the recommendations of the Clinical Management of Tinnitus Guidelines, and 3% provided literature referenced by the video. A variety types of sound were used, among which music accounting for 35%. The videos were highly popular with an average views of 7,335,003.28 ± 24,174,764.02 and an average VPI of 4,610.33 ± 11,531.10. However, their quality was poor (the median scores: 38/80 for DISCERN, 2/5 for GQS, 1/4 for JAMA, and 50%/100% for PEMAT). There was a negative correlation between the popularity of the videos and their quality, indicated by PEMAT: -0.207, DISCERN: -0.307, GQS: -0.302, and JAMA: -0.233. Several dimensions of the videos require improvement, especially actionability, treatment options, and transparency with lacks of 100%, 63%, and 75% respectively. Conclusion: The tinnitus sound therapy videos available on YouTube exhibit low quality. Nevertheless, they also hold potential for health education if refined and utilized suitably.
MeSH term(s)	United States ; Humans ; Reproducibility of Results ; Social Media ; Tinnitus/diagnosis ; Patient Education as Topic ; American Medical Association
Language	English
Publishing date	2024-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.16846
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Article ; Online: Evaluation of the current situation and quality of neonatal hearing screening from hearing screening practitioners' perspective: a cross-sectional study.

Lan, Hongli / Liu, Maojie / Huang, Chao / Ren, Jing / Huang, Yu / Jiang, Fan / Lai, Dan

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

2024 Volume 37, Issue 1, Page(s) 2317412

Abstract: Background: In recent years, neonatal hearing screening (NHS) has gained rapid traction in both developed and developing nations. However, the efficacy of these efforts depends on comprehensive standardization across all screening facets. This study ... ...

Abstract	Background: In recent years, neonatal hearing screening (NHS) has gained rapid traction in both developed and developing nations. However, the efficacy of these efforts depends on comprehensive standardization across all screening facets. This study aimed to assess the status and quality of NHS by investigating the knowledge, attitudes, beliefs, and practices of hearing screening practitioners regarding NHS. Methods: A cross-sectional survey was conducted, and an online questionnaire based on the knowledge-attitude/belief ( Results: A total of 63 valid questionnaires were collected. The practitioners were mainly female (96.83%), with nursing backgrounds (63.49%), and undergraduate degrees (66.67%). Most had ≤5 years of experience (74.60%) and had junior/intermediate titles (93.65%). The NHS within the Luzhou area started in 2006 with provincial institutions, expanding to 42 institutions by 2022. Statistically significant correlations were observed between the Conclusions: It has been 17 years since the first medical institution in Luzhou launched NHS, and the overall performance of practitioners from different institutions has been consistent in terms of their knowledge, attitudes, or level of practice. However, there is room for further improvement in both the professional development of individuals and aspects related to work, such as health education and long-term follow-up.
MeSH term(s)	Infant, Newborn ; Humans ; Female ; Male ; Cross-Sectional Studies ; Neonatal Screening ; Hearing Tests ; Health Knowledge, Attitudes, Practice ; Surveys and Questionnaires ; Hearing
Language	English
Publishing date	2024-02-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2077261-0
ISSN	1476-4954 ; 1057-0802 ; 1476-7058
ISSN (online)	1476-4954
ISSN	1057-0802 ; 1476-7058
DOI	10.1080/14767058.2024.2317412
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Zs.A 3510: Show issues

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Article ; Online: SARS-CoV-2 spike linear epitope scanning via a peptide microarray through sera profiling.

Li, Yang / Lai, Dan-Yun / Tao, Sheng-Ce

STAR protocols

2021 Volume 2, Issue 3, Page(s) 100707

Abstract: Host humoral immunological response plays an essential role in protection against pathogens. Identification of B-cell epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 (severe acute respiratory syndrome ... ...

Abstract	Host humoral immunological response plays an essential role in protection against pathogens. Identification of B-cell epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) spike linear epitopes, we developed a protocol of profiling sera from patients with COVID-19 (coronavirus disease 2019) via a peptide microarray designed according to spike protein. The protocol is also applicable for other antigens or sample types. This protocol is rapid, high throughput, and the cost is acceptable while it needs specialized microarray facilities. For complete details on the use and execution of this protocol, please refer to Li et al. (2020, 2021a, 2021b).
MeSH term(s)	Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Antigens, Viral/blood ; Antigens, Viral/immunology ; COVID-19/blood ; COVID-19/immunology ; COVID-19/virology ; Epitopes, B-Lymphocyte/immunology ; Humans ; Peptide Fragments/blood ; Peptide Fragments/immunology ; SARS-CoV-2/immunology ; Spike Glycoprotein, Coronavirus/blood ; Spike Glycoprotein, Coronavirus/immunology
Chemical Substances	Antibodies, Viral ; Antigens, Viral ; Epitopes, B-Lymphocyte ; Peptide Fragments ; Spike Glycoprotein, Coronavirus
Language	English
Publishing date	2021-07-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2021.100707
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Article ; Online: Combined evaluation of audiology examination and self-reported symptoms in patients with hyperacusis.

Huang, Yu / Xiang, Tao / Jiang, Fan / Ren, Jing / Xu, Tao / Lai, Dan

Scientific reports

2023 Volume 13, Issue 1, Page(s) 1522

Abstract: To investigate the application of combined audiological examination and a self-reported symptoms survey in the evaluation of hyperacusis. Patients who visited the outpatient department of Otolaryngology Head and Neck Surgery and Otological medicine, ... ...

Abstract	To investigate the application of combined audiological examination and a self-reported symptoms survey in the evaluation of hyperacusis. Patients who visited the outpatient department of Otolaryngology Head and Neck Surgery and Otological medicine, Affiliated Hospital of Southwest Medical University, from January 2019 to March 2021 were divided into a hyperacusis group and a normal control group. We measured the loudness discomfort level (LDL) and hearing threshold (HT) of the subjects and investigated their self-reported symptoms. We compared the demographic characteristics, loudness discomfort level, and hearing threshold of the two groups and analyzed the self-reported symptoms and audiological characteristics of hyperacusis. We considered 87 subjects, comprising 40 patients with hyperacusis and 47 healthy individuals. Among the hyperacusis patients, bilateral disease was predominant. Among them, 33 were females, 23 had hearing loss, and 20 had tinnitus. Patients are mainly in the 21-60 age group. Patients with hyperacusis had low discomfort thresholds at all frequencies except 500 Hz (P ≤ 0.05, mean LDL decreased by 6.14-1.37 dB HL for all frequencies). The incidences of feeling upset, pain, and anxiety or fear were 95%, 65%, and 82.5%, respectively, in patients with hyperacusis. The severity of symptoms varies between patients with hyperacusis and healthy individuals. A combination of LDL measurements and self-reported symptom surveys allows for an accurate and comprehensive assessment of hyperacusis.Trial registration: This study was retrospectively registered (TRN: ChiCTR2100047391) on June 13, 2021.
MeSH term(s)	Female ; Humans ; Male ; Hyperacusis/diagnosis ; Self Report ; Audiology ; Hearing ; Tinnitus/diagnosis ; Tinnitus/epidemiology ; Outpatients
Language	English
Publishing date	2023-01-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-28570-8
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Article: Management of the palato-radicular groove with a periodontal regenerative procedure and prosthodontic treatment: A case report.

Ling, Dan-Hua / Shi, Wei-Ping / Wang, Yan-Hong / Lai, Dan-Ping / Zhang, Yan-Zhen

World journal of clinical cases

2022 Volume 10, Issue 17, Page(s) 5732–5740

Abstract: Background: Palato-radicular groove (PRG) is defined as an anomalous formation of teeth. The etiology of PRG remains unclear. The prognosis of a tooth with a PRG is unfavorable. The treatment of combined periodontal-endodontic lesions requires ... ...

Abstract	Background: Palato-radicular groove (PRG) is defined as an anomalous formation of teeth. The etiology of PRG remains unclear. The prognosis of a tooth with a PRG is unfavorable. The treatment of combined periodontal-endodontic lesions requires multidisciplinary management to control the progression of bone defects. Some researchers reported cases that had short-term observations. The management of teeth with PRGs is of great clinical significance. However, to date, no case reports have been documented on the use of bone regeneration and prosthodontic treatment for PRGs. Case summary: This case reported the management of a 40-year-old male patient with the chief complaint of slight mobility and abscess in the upper right anterior tooth for 15 d and was diagnosed with type II PRG of tooth 12 with combined endodontic-periodontal lesions. The accumulation of plaque and calculus caused primary periodontitis and a secondary endodontic infection. A multidisciplinary management approach was designed that included root canal therapy, groove sealing, a periodontal regenerative procedure, and prosthodontic treatment. During a 2-year follow-up period, a good prognosis was observed. Conclusion: This report indicates that bone regeneration and prosthodontic treatment may contribute to the long-term favorable prognosis of teeth with PRGs.
Language	English
Publishing date	2022-08-01
Publishing country	United States
Document type	Case Reports
ISSN	2307-8960
ISSN	2307-8960
DOI	10.12998/wjcc.v10.i17.5732
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Article ; Online: SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling.

Lai, Dan-Yun / Jiang, He-Wei / Li, Yang / Zhang, Hai-Nan / Tao, Sheng-Ce

STAR protocols

2022 Volume 3, Issue 2, Page(s) 101238

Abstract: The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by ... ...

Abstract	The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either
MeSH term(s)	Antibodies, Viral ; COVID-19 ; Escherichia coli ; Humans ; Proteome ; SARS-CoV-2
Chemical Substances	Antibodies, Viral ; Proteome
Language	English
Publishing date	2022-02-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2666-1667
ISSN (online)	2666-1667
DOI	10.1016/j.xpro.2022.101238
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Article ; Online: Current advances in antibody-based serum biomarker studies: From protein microarray to phage display.

Qi, Huan / Xue, Jun-Biao / Lai, Dan-Yun / Li, Ang / Tao, Sheng-Ce

Proteomics. Clinical applications

2022 Volume 16, Issue 6, Page(s) e2100098

Abstract: Purpose: This review aims to summarize the technological advances in the field of antibody-based biomarker studies by proteome microarray and phage display. In addition, the possible development directions of this field are also discussed.: ... ...

Abstract	Purpose: This review aims to summarize the technological advances in the field of antibody-based biomarker studies by proteome microarray and phage display. In addition, the possible development directions of this field are also discussed. Experimental design: We have focused on the antibody profiling by proteome microarray and phage display, including the technological advances, the tools/resources constructed, and the characteristics of both platforms. Results: With the help of tools/resources and technological advances in proteome microarray and phage display, the efficiency of profiling antibody-based biomarkers in serum samples has been greatly improved. Conclusions: In the past few years, proteome microarray and phage display, especially the latter one, have already demonstrated their capacity and efficiency for biomarker identification. In the near future, we believe that more antibody-based biomarkers could be identified, and some of them could eventually be developed into real clinical applications.
MeSH term(s)	Protein Array Analysis ; Proteome ; Antibodies/genetics ; Biomarkers ; Bacteriophages/genetics ; Peptide Library
Chemical Substances	Proteome ; Antibodies ; Biomarkers ; Peptide Library
Language	English
Publishing date	2022-09-27
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	2261788-7
ISSN	1862-8354 ; 1862-8346
ISSN (online)	1862-8354
ISSN	1862-8346
DOI	10.1002/prca.202100098
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Zs.A 6575: Show issues

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Article ; Online: Intratympanic glucocorticosteroid therapy for idiopathic sudden hearing loss: Meta-analysis of randomized controlled trials.

Lai, Dan / Zhao, Fei / Jalal, Nasim / Zheng, Yun

Medicine

2018 Volume 96, Issue 50, Page(s) e8955

Abstract: Background and objective: Glucocorticoids are the standard treatment for idiopathic sudden sensorineural hearing loss (ISSNHL), but whether intratympanic or systemic therapy is superior remains controversial. Previous meta-analyses of this question have ...

Abstract	Background and objective: Glucocorticoids are the standard treatment for idiopathic sudden sensorineural hearing loss (ISSNHL), but whether intratympanic or systemic therapy is superior remains controversial. Previous meta-analyses of this question have omitted key clinical trials or included observational studies. Methods: English-language randomized controlled trials in OvidSP, PubMed, Embase, CINAHL, and the Cochrane Library comparing intratympanic versus systemic glucocorticoid therapy for ISSNHL were meta-analyzed using RevMan 5.3. The primary outcome of interest was improvement in pure tone average (PTA) threshold. Results: Six trials involving 248 patients receiving intratympanic steroids and 236 receiving systemic steroids were meta-analyzed. PTA thresholds were similar between the 2 groups at 3 months after therapy initiation (mean difference, 0.24; 95% confidence interval [CI] -2.43 to 2.91, P = .86; I = 54%, P = .07, random-effects model). PTA thresholds were also similar at 6 months (mean difference, 4.69, 95% CI -5.84 to 15.22, P = .38), although the results showed extremely high heterogeneity (I = 98%). Sensitivity analysis indicated that a single trial containing 250 patients provided the strongest evidence for equivalence between the 2 types of therapy. Rates of recovery within 3 months (defined as PTA improvement >10 dB) were similar between the 2 types of therapy (odds ratio 0.92, 95% CI 0.59-1.43, P = .70), with no significant heterogeneity in the pooled data (I = 1%, P = .40). Conclusion: Intratympanic and systemic steroids' therapies appear to show similar short-term efficacy for restoring hearing in patients with ISSNHL. Intratympanic therapy may reduce systemic side effects associated with steroid use.
MeSH term(s)	Glucocorticoids/therapeutic use ; Hearing Loss, Sensorineural/drug therapy ; Hearing Loss, Sudden/drug therapy ; Humans ; Injection, Intratympanic ; Randomized Controlled Trials as Topic
Chemical Substances	Glucocorticoids
Language	English
Publishing date	2018-01-29
Publishing country	United States
Document type	Journal Article ; Meta-Analysis
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000008955
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Article: Ma-Huang-Fu-Zi-Xi-Xin Decoction for Allergic Rhinitis: A Systematic Review.

Zhong, Juan / Lai, Dan / Zheng, Yun / Li, Gang

Evidence-based complementary and alternative medicine : eCAM

2018 Volume 2018, Page(s) 8132798

Abstract: Background: The treatment effects and safety of Ma-Huang-Fu-Zi-Xi-Xin decoction for patients with allergic rhinitis have yet to be clarified.: Objectives: The aim of this study is to evaluate the effects and safety of Ma-Huang-Fu-Zi-Xi-Xin decoction ... ...

Abstract	Background: The treatment effects and safety of Ma-Huang-Fu-Zi-Xi-Xin decoction for patients with allergic rhinitis have yet to be clarified. Objectives: The aim of this study is to evaluate the effects and safety of Ma-Huang-Fu-Zi-Xi-Xin decoction in patients with allergic rhinitis. Methods: We searched PubMed, EMBASE (Excerpta Medical Database), Cochrane Library, Chinese Cochrane Centre's Controlled Trials Register Platform, Wanfang Chinese Digital Periodical and Conference Database, China National Knowledge Infrastructure (CNKI) Database, and VIP Chinese Science and Technique Journals Database to collect randomized controlled trials of Ma-Huang-Fu-Zi-Xi-Xin decoction (MHFZXXD) for allergic rhinitis (AR) prior to May 8, 2017. RevMan 5.3 software was used to conduct a meta-analysis. GRADE methodology was applied to evaluate the evidence quality for each outcome. Results: Six randomized controlled trials (RCTs) involving 576 participants (10-78 years old) were included. A meta-analysis revealed that the overall effect of MHFZXXD for AR was not better than western medical treatment (RR: 0.41; 95% CI: 0.26-0.65;
Language	English
Publishing date	2018-02-05
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2018/8132798
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Zs.A 5995: Show issues

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Article: [Effect of hydrogen peroxide on electric current of large-conductance calcium-activated potassium channel in isolated outer hair cells of old guinea pig cochlea].

Lai, Dan / Huang, Fei

Sheng li xue bao : [Acta physiologica Sinica

2014 Volume 66, Issue 3, Page(s) 302–306

Abstract: The present study was aimed to investigate the effect of hydrogen peroxide (H₂O₂, oxygen free radical donator) on the current of large-conductance calcium-activated potassium channels (BK(Ca) channels) in isolated outer hair cells of old guinea pig ... ...

Abstract	The present study was aimed to investigate the effect of hydrogen peroxide (H₂O₂, oxygen free radical donator) on the current of large-conductance calcium-activated potassium channels (BK(Ca) channels) in isolated outer hair cells of old guinea pig cochlea, and to explore the underlying mechanism. Outer hair cells of old guinea pig cochlea were acutely enzyme-isolated, and currents were recorded by whole-cell patch clamp. The results showed that, rapid activation and non-deactivation electric currents with a string of large amplitude were recorded. Activation voltage of the current was above -40 - -30 mV. The amplitude of current was increased continuously with the rising of membrane potential. The current showed characteristics of outward rectification without "rundown" phenomenon. IbTX (100 nmol/L) could completely block the activity of channel, which confirmed BK(Ca) channel's current. BK(Ca) current amplitude and peak current density increased with the increment of H₂O₂ concentration (1, 2, 4 μmol/L), showing concentration-dependent activation by H₂O₂. Our results suggest that oxygen free radical/BK(Ca) pathway may be able to adjust the balance of intracellular calcium in outer hair cells.
MeSH term(s)	Animals ; Calcium/metabolism ; Cochlea/cytology ; Guinea Pigs ; Hair Cells, Vestibular/drug effects ; Hydrogen Peroxide/pharmacology ; Large-Conductance Calcium-Activated Potassium Channels/metabolism ; Membrane Potentials
Chemical Substances	Large-Conductance Calcium-Activated Potassium Channels ; Hydrogen Peroxide (BBX060AN9V) ; Calcium (SY7Q814VUP)
Language	Chinese
Publishing date	2014-06-25
Publishing country	China
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604308-2
ISSN	0371-0874
ISSN	0371-0874
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