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Article: The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat.

Laksmitawati, Dian Ratih / Noor, Siti Umrah / Sumiyati, Yati / Hartanto, Adrian / Widowati, Wahyu / Pratami, Diah Kartika

Veterinary world

2022 Volume 15, Issue 4, Page(s) 841–847

Abstract: Background and aim: Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and ... ...

Abstract	Background and aim: Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and culture medium/conditioned medium (CM), altering tissue physiology. These factors can help wound healing, but their effect on third-degree burns is poorly understood. This research aimed to study the activity of mesenchymal stem cell-conditioned medium gel in healing and repairing third-degree burns on rats skin. Materials and methods: Twenty-four Sprague-Dawley rats with burn wounds on the dorsal area were divided into four groups; the first group was treated with CM gel, with a concentration equivalent to 0.05% protein, the second group was treated with a placebo gel, the third group with silver sulfadiazine (SSD) cream (SSD-Burnazin contain 10 mg/g SSD), and the fourth group was not given any treatment, for 21 days, and on the final day, the rats were sacrificed, and the skins were taken. All topical treatments completely cover the wound area. Results: Wound healing process indicators observed include wound diameter, scabs' formation, blister formation, and hair growth every day. The skins taken were processed with hematoxylin-eosin and Masson's trichrome staining. The indicators studied include neutrophil infiltration, mononuclear cell infiltration, neovascularization, collagen area, and re-epithelization ratio. Conclusion: CM shows better wound healing than other groups and faster hair growth.
Language	English
Publishing date	2022-04-07
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2022.841-847
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Article: In-vitro antioxidant, in-vivo anti-inflammatory, and acute toxicity study of Indonesian propolis capsule from

Farida, Siti / Pratami, Diah Kartika / Sahlan, Muhamad / Laksmitawati, Dian Ratih / Rohmatin, Etin / Situmorang, Herbert

Saudi journal of biological sciences

2021 Volume 29, Issue 4, Page(s) 2489–2500

Abstract: Propolis is widely used as traditional medicine since ancient times. It was necessary to conduct the pre-clinical study because of its relevant curative properties. This study aimed to investigate in-vitro antioxidant, standardize quality parameters, ... ...

Abstract	Propolis is widely used as traditional medicine since ancient times. It was necessary to conduct the pre-clinical study because of its relevant curative properties. This study aimed to investigate in-vitro antioxidant, standardize quality parameters, study acute toxicity, and determine in-vivo anti-inflammatory. Three spectrophotometric methods were used to determine antioxidant activity. The standardization includes physical, chemical, and microbiological evaluation. Furthermore, an acute toxicity test was conducted using 20 female Sprague Dawley (SD) strain rats divided into 4 groups with different dose of propolis. The in vivo anti-inflammatory test was carried out using the carrageenan induction method on rats' soles. A total of 36 female SD rats were classified into 6 groups as follows, Group normal, negative control, diclofenac sodium, and three propolis groups (72; 144; and 288 mg/kg BW). The results demonstrated the IC
Language	English
Publishing date	2021-12-16
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2021.12.034
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Article ; Online: Production of Inflammatory Mediators in Conditioned Medium of Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC)-Treated Fresh Frozen Plasma.

Laksmitawati, Dian Ratih / Widowati, Wahyu / Noverina, Rachmawati / Ayuningtyas, Wireni / Kurniawan, Dedy / Kusuma, Hanna Sari Widya / Afifah, Ervi / Rinendyaputri, Ratih / Rilianawati, Rilianawati / Faried, Ahmad / Susilarini, Ni Ketut

Medical science monitor basic research

2022 Volume 28, Page(s) e933726

Abstract: BACKGROUND Inflammation is the body's first response to an illness that causes irritation or infection. Inflammation is tightly correlated with aging, which is a progressive degenerative process. Conditioned medium (CM) from adipose tissue-derived ... ...

Abstract	BACKGROUND Inflammation is the body's first response to an illness that causes irritation or infection. Inflammation is tightly correlated with aging, which is a progressive degenerative process. Conditioned medium (CM) from adipose tissue-derived mesenchymal stem cells (CM-ATMSCs) has been shown to stimulate collagen synthesis and dermal fibroblast migration, as well as reduce wrinkles and improve wound healing. This study aimed to observe the production of inflammatory modulators - interleukin (IL)-1alpha, IL-6, IL-10, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) - in CM-ATMSCs treated with fresh frozen plasma (FFP) at passages 3 (P3), 7, 11, and 15. MATERIAL AND METHODS ATMSCs P3 were obtained from liposuction of female donors, and the CM from ATMSCs was collected. Measurement of these cytokines was performed with ELISA. RESULTS At many passages, IL-6, a proinflammatory modulator, was discovered to be the most powerful modulator among FFP- and non-FFP-treated cells. However, CM-ATMSCs treated with FFP and in the late passage have significant differences (P<0.05) compared to non-FFP treatments and in other passages in their effects on secretion of inflammatory modulators. CONCLUSIONS In conclusion, CM-ATMSC has the potential to secrete proinflammatory modulators.
MeSH term(s)	Adipose Tissue ; Culture Media, Conditioned/pharmacology ; Female ; Humans ; Inflammation Mediators ; Mesenchymal Stem Cells/physiology ; Plasma
Chemical Substances	Culture Media, Conditioned ; Inflammation Mediators
Language	English
Publishing date	2022-03-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2711344-9
ISSN	2325-4416 ; 2325-4416
ISSN (online)	2325-4416
ISSN	2325-4416
DOI	10.12659/MSMBR.933726
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Article: Decreased Inhibition of Proliferation and Induction of Apoptosis in Breast Cancer Cell Lines (T47D and MCF7) from Treatment with Conditioned Medium Derived from Hypoxia-Treated Wharton's Jelly MSCs Compared with Normoxia-Treated MSCs.

Widowati, Wahyu / Murti, Harry / Widyastuti, Halida / Laksmitawati, Dian Ratih / Rizal, Rizal / Widya Kusuma, Hanna Sari / Sumitro, Sutiman Bambang / Widodo, M Aris / Bachtiar, Indra

International journal of hematology-oncology and stem cell research

2021 Volume 15, Issue 2, Page(s) 77–89

Abstract: ... ...

Abstract	Background
Language	English
Publishing date	2021-08-27
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2652853-8
ISSN	2008-2207 ; 2008-3009
ISSN (online)	2008-2207
ISSN	2008-3009
DOI	10.18502/ijhoscr.v15i2.6038
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Article: In vitro

Sandhiutami, Ni Made Dwi / Moordiani, Moordiani / Laksmitawati, Dian Ratih / Fauziah, Nurul / Maesaroh, Maesaroh / Widowati, Wahyu

Iranian journal of basic medical sciences

2017 Volume 20, Issue 1, Page(s) 99–106

Abstract: Objectives: Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer's and ... ...

Abstract	Objectives: Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer's and various cancer. Indonesian cassia ( Materials and methods: The cytotoxic assay of coumarin and ICE against RAW264.7 cells was conducted using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). The anti-inflammatory potential was determined using LPS-induced RAW 267.4 macrophages cells to measure inhibitory activity of compound and ISEon production of nitric oxide (NO), prostaglandin E2 (PGE2), and also cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and TNF-α. Results: Coumarin 10 µM and ICE 10 µg/ml were nontoxic to the RAW264.7 cells. Both of coumarin and ICE were capable to reduce the PGE2, TNF-α, NO, IL-6, and IL-β level in LPS-induced RAW264.7 cells. Coumarin had higher activity to decrease PGE2 and TNF-α, whilst ICE had higher activity to inhibit NO, IL-6, and IL-β levels. Conclusion: Coumarin and ICE possess anti-inflammatory properties through inhibition of PGE2 and NO along with pro-inflammatory cytokines TNF-α, IL-6, IL-1β production.
Language	English
Publishing date	2017-01-15
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2500485-2
ISSN	2008-3874 ; 2008-3866
ISSN (online)	2008-3874
ISSN	2008-3866
DOI	10.22038/ijbms.2017.8102
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Article: Mangosteen peel extract (

Widowati, Wahyu / Laksmitawati, Dian Ratih / Wargasetia, Teresa Liliana / Afifah, Ervi / Amalia, Annisa / Arinta, Yukko / Rizal, Rizal / Suciati, Tri

Iranian journal of basic medical sciences

2018 Volume 21, Issue 9, Page(s) 972–977

Abstract: Objectives: This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (: Materials and methods: MPE was performed based on maceration method. Cytotoxic ... ...

Abstract	Objectives: This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line ( Materials and methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT. Results: In viability assay, MPE 5 and 20 µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) ( Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β1 and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation.
Language	English
Publishing date	2018-11-22
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2500485-2
ISSN	2008-3874 ; 2008-3866
ISSN (online)	2008-3874
ISSN	2008-3866
DOI	10.22038/IJBMS.2018.29349.7094
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Article: Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma.

Widowati, Wahyu / Noverina, Rachmawati / Ayuningtyas, Wireni / Kurniawan, Dedy / Kusuma, Hanna Sari Widya / Arumwardana, Seila / Artie, Dwi Surya / Sholihah, Ika Adhani / Handayani, Rr Anisa Siwianti / Laksmitawati, Dian Ratih / Rinendyaputri, Ratih / Rilianawati, Rilianawati / Faried, Ahmad

International journal of molecular and cellular medicine

2020 Volume 8, Issue 4, Page(s) 283–294

Abstract: Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research is aimed to ... ...

Abstract	Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research is aimed to determine the proliferation, characteristics and differentiation capacity of adipose tissue-derived (AT)-MSCs at many passages with different media. The cell proliferation capacity was assayed using trypan blue. MSCs characterization (CD90, CD44, CD105, CD73, CD11b, CD19, CD34, CD45, and HLA-DR) was performed by flow cytometry, and cell differentiation was determined by specific stainings. Population doubling time (PDT) of AT-MSCs treated with fresh frozen plasma (FFP) and non-FFP increased in the late passage (P) (P15 FFP was 22.67 ± 7.01 days and non-FFP was 19.65 ± 2.27 days). Cumulative cell number was significantly different between FFP and non-FFP at P5, 10, 15. AT-MSCs at P4-15 were positive for CD90, CD44, CD105, and CD73, and negative for CD11b, CD19, CD34, CD45, and HLA-DR surface markers. AT-MSCs at P5, 10, 15 had potential toward adipogenic, chondrogenic, and osteogenic differentiation. Therefore, PDT was affected by increased age but no difference was observed in morphology, surface markers and differentiation capacity among passages. Cumulative cell number in FFP was higher in comparison with non-FFP in P5, 10, 15. Our data suggest that FFP may replace FBS for culturing MSCs.
Language	English
Publishing date	2020-06-10
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2697015-6
ISSN	2251-9645 ; 2251-9637
ISSN (online)	2251-9645
ISSN	2251-9637
DOI	10.22088/IJMCM.BUMS.8.4.283
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Article: Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients.

Widowati, Wahyu / Heriady, Yusuf / Laksmitawati, Dian Ratih / Jasaputra, Diana Krisanti / Wargasetia, Teresa Liliana / Rizal, Rizal / Perdana, Fajar Sukma / Amalia, Annisa / Arlisyah, Annisa / Khoiriyah, Zakiyatul / Faried, Ahmad / Subangkit, Mawar

Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH

2019 Volume 26, Issue 4, Page(s) 240–244

Abstract: Introduction: The most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of ... ...

Abstract	Introduction: The most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of therapy in model cell of breast cancer. Aim: This study aim to develop an isolation technique of breast cancer cell from patients as a cancer cell model. Material and methods: Breast cancer cell isolation is performed by enzymatic methods using the collagen I and hyaluronidase. Then, breast cancer cells were characterized using flow cytometry based on the CD44/CD24 expression where MDA-MB468 and MCF-7 breast cancer cell lines were used as positive controls. Estrogen receptor (ER), progesterone receptor (PR), p53, HER2, and Ki67 expression were assessed using an immunohistochemistry assay. Result and discussion: The morphology of cancer cells was fibroblast like cells on the day 7 Conclusion: These cells belonged to a basal type of breast carcinoma and expressed CD44
Language	English
Publishing date	2019-01-28
Publishing country	Bosnia and Herzegovina
Document type	Journal Article
ZDB-ID	2558601-4
ISSN	1986-5988 ; 0353-8109
ISSN (online)	1986-5988
ISSN	0353-8109
DOI	10.5455/aim.2018.26.240-244
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Article: Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity.

Widowati, Wahyu / Widyanto, Rahma Micho / Husin, Winsa / Ratnawati, Hana / Laksmitawati, Dian Ratih / Setiawan, Bambang / Nugrahenny, Dian / Bachtiar, Indra

Iranian journal of basic medical sciences

2015 Volume 17, Issue 9, Page(s) 702–709

Abstract: Objectives: Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green tea is known to have potent antioxidant and free radical scavenging activities. This study aimed to investigate whether ... ...

Abstract	Objectives: Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green tea is known to have potent antioxidant and free radical scavenging activities. This study aimed to investigate whether green tea extract (GTE) can protect endothelial progenitors cells (EPCs) against oxidative stress through antioxidant mechanisms. Materials and methods: Mononuclear cells (MNCs) were isolated from peripheral blood by density gradient centrifugation with Ficoll. The cells were then plated on fibronectin-coated culture dishes. After 7 days of culture, EPCs were characterized as adherent cells double positive for DiI-ac-LDL uptake and lectin binding. EPCs were further identified by assessing the expression of CD34/45, CD133, and KDR. EPCs were then treated with hydrogen peroxide (H2O2) at doses of 50, 100, 200 µM and incubated with or without GTE (25 µg/ml). The intracellular reactive oxygen species (ROS) levels were detected by flow cytometry using a 2',7'-dichlorofluorescein diacetate (DCF-DA) fluorescent probe. Results: GTE ameliorated the cell viability of EPCs induced by H2O2 at doses of 50, 100, 200 µM for about 25.47, 22.52, and 11.96% higher than controls, respectively. GTE also decreased the intracellular ROS levels of EPCs induced by H2O2 at doses of 50, 100, 200 µM for about 84.24, 92.27, and 93.72% compared to controls, respectively. Conclusion: GTE improves cell viability by reducing the intracellular ROS accumulation in H2O2-induced EPCs.
Language	English
Publishing date	2015-02-07
Publishing country	Iran
Document type	Journal Article
ZDB-ID	2500485-2
ISSN	2008-3874 ; 2008-3866
ISSN (online)	2008-3874
ISSN	2008-3866
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Article: The effect of mesenchymal stem cell-conditioned medium gel on burn wound healing in rat.

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Article: In-vitro antioxidant, in-vivo anti-inflammatory, and acute toxicity study of Indonesian propolis capsule from

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Article ; Online: Production of Inflammatory Mediators in Conditioned Medium of Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC)-Treated Fresh Frozen Plasma.

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Article: Decreased Inhibition of Proliferation and Induction of Apoptosis in Breast Cancer Cell Lines (T47D and MCF7) from Treatment with Conditioned Medium Derived from Hypoxia-Treated Wharton's Jelly MSCs Compared with Normoxia-Treated MSCs.

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Article: In vitro

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Article: Mangosteen peel extract (

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Article: Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma.

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Article: Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients.

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Article: Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity.

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