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Article: Post-traumatic thrombosis of the axillary vein.

LAMOUREUX, M

2010 Volume 76, Issue 4, Page(s) 491

Title translation	Thrombose post-traumatique de la veine axillaire.
MeSH term(s)	Axilla ; Axillary Vein ; Humans ; Thrombosis
Language	French
Publishing date	2010-02-28
Publishing country	Canada
Document type	Journal Article
ZDB-ID	390006-x
ISSN	0041-6959 ; 0701-2543
ISSN	0041-6959 ; 0701-2543
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Article ; Online: Real world external validation of metabolic gestational age assessment in Kenya.

Hawken, Steven / Ward, Victoria / Bota, A Brianne / Lamoureux, Monica / Ducharme, Robin / Wilson, Lindsay A / Otieno, Nancy / Munga, Stephen / Nyawanda, Bryan O / Atito, Raphael / Stevenson, David K / Chakraborty, Pranesh / Darmstadt, Gary L / Wilson, Kumanan

PLOS global public health

2022 Volume 2, Issue 11, Page(s) e0000652

Abstract: Using data from Ontario Canada, we previously developed machine learning-based algorithms incorporating newborn screening metabolites to estimate gestational age (GA). The objective of this study was to evaluate the use of these algorithms in a ... ...

Abstract	Using data from Ontario Canada, we previously developed machine learning-based algorithms incorporating newborn screening metabolites to estimate gestational age (GA). The objective of this study was to evaluate the use of these algorithms in a population of infants born in Siaya county, Kenya. Cord and heel prick samples were collected from newborns in Kenya and metabolic analysis was carried out by Newborn Screening Ontario in Ottawa, Canada. Postnatal GA estimation models were developed with data from Ontario with multivariable linear regression using ELASTIC NET regularization. Model performance was evaluated by applying the models to the data collected from Kenya and comparing model-derived estimates of GA to reference estimates from early pregnancy ultrasound. Heel prick samples were collected from 1,039 newborns from Kenya. Of these, 8.9% were born preterm and 8.5% were small for GA. Cord blood samples were also collected from 1,012 newborns. In data from heel prick samples, our best-performing model estimated GA within 9.5 days overall of reference GA [mean absolute error (MAE) 1.35 (95% CI 1.27, 1.43)]. In preterm infants and those small for GA, MAE was 2.62 (2.28, 2.99) and 1.81 (1.57, 2.07) weeks, respectively. In data from cord blood, model accuracy slightly decreased overall (MAE 1.44 (95% CI 1.36, 1.53)). Accuracy was not impacted by maternal HIV status and improved when the dating ultrasound occurred between 9 and 13 weeks of gestation, in both heel prick and cord blood data (overall MAE 1.04 (95% CI 0.87, 1.22) and 1.08 (95% CI 0.90, 1.27), respectively). The accuracy of metabolic model based GA estimates in the Kenya cohort was lower compared to our previously published validation studies, however inconsistency in the timing of reference dating ultrasounds appears to have been a contributing factor to diminished model performance.
Language	English
Publishing date	2022-11-28
Publishing country	United States
Document type	Journal Article
ISSN	2767-3375
ISSN (online)	2767-3375
DOI	10.1371/journal.pgph.0000652
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Article ; Online: Fluorescence labelling of DNA by carboxylic polypyridyl-Ru complexes containing bpy and DIP ligands: a study revisited.

Lamoureux, Michèle / Seksek, Olivier

Journal of fluorescence

2010 Volume 20, Issue 3, Page(s) 631–643

Abstract: The coordination complexes (DIP)(2)Ru(CH(3)bpyCOOH) and (DIP)(2)Ru(COOHbpyCOOH), where DIP and bpy are diphenylphenanthroline and bispyridine, have been recently proposed as fluorescent markers of nuclear DNA (Musatkina et al., J. Inorg. Biochem. 101: ... ...

Abstract	The coordination complexes (DIP)(2)Ru(CH(3)bpyCOOH) and (DIP)(2)Ru(COOHbpyCOOH), where DIP and bpy are diphenylphenanthroline and bispyridine, have been recently proposed as fluorescent markers of nuclear DNA (Musatkina et al., J. Inorg. Biochem. 101:1086-1089, 2007), but no DNA binding investigation and no quantitative fluorescence evaluations had been done. Both complexes, as well as the smaller ones with bpy's in place of DIP's, have been investigated here by spectroscopic DNA titrations (UV-vis absorption, fluorescence, circular dichroism) and by in vitro cellular studies (flow cytometry and fluorescence imaging). Contrary to previous reports, neither the carboxylic function nor the more extended DIP ligand ensures any appreciable binding to DNA. This is clearly illustrated by the appearance of an isosbestic point of a second kind and by the proportionality of the fluorescence maximum intensity to the absorbance at the excitation wavelength. Above all, the lack of enhanced fluorescence in the presence of DNA definitively rules out the use of such complexes as DNA markers. Moreover, there is no detectable nuclear uptake. However, the fluorescent complexes with the DIP ligands, especially (DIP)(2)Ru(CH(3)bpyCOOH), are massively incorporated into the cytoplasm while preserving cell integrity, which could suggest other types of biological application.
MeSH term(s)	Absorption ; Circular Dichroism ; Coordination Complexes ; DNA/chemistry ; DNA/metabolism ; Fluorescence ; Ligands ; Salicylates ; Spectrum Analysis
Chemical Substances	Coordination Complexes ; Ligands ; Salicylates ; 3,5-diisopropylsalicylic acid (2215-21-6) ; DNA (9007-49-2)
Language	English
Publishing date	2010-04-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2016892-5
ISSN	1573-4994 ; 1053-0509
ISSN (online)	1573-4994
ISSN	1053-0509
DOI	10.1007/s10895-009-0592-6
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Article ; Online: Metabolic Signatures of Cystic Fibrosis Identified in Dried Blood Spots For Newborn Screening Without Carrier Identification.

DiBattista, Alicia / McIntosh, Nathan / Lamoureux, Monica / Al-Dirbashi, Osama Y / Chakraborty, Pranesh / Britz-McKibbin, Philip

Journal of proteome research

2019 Volume 18, Issue 3, Page(s) 841–854

Abstract: Cystic fibrosis (CF) is a complex multiorgan disorder that is among the most common fatal genetic diseases benefiting from therapeutic interventions early in life. Newborn screening (NBS) for presymptomatic detection of CF currently relies on a two-stage ...

Abstract	Cystic fibrosis (CF) is a complex multiorgan disorder that is among the most common fatal genetic diseases benefiting from therapeutic interventions early in life. Newborn screening (NBS) for presymptomatic detection of CF currently relies on a two-stage immunoreactive trypsinogen (IRT) and cystic fibrosis transmembrane conductance regulator (CFTR) mutation panel algorithm that is sensitive but not specific for identifying affected neonates with a low positive predictive value. For the first time, we report the discovery of a panel of CF-specific metabolites from a single 3.2 mm diameter dried blood spot (DBS) punch when using multisegment injection-capillary electrophoresis-mass spectrometry (MS) as a high-throughput platform for nontargeted metabolite profiling from volume-restricted/biobanked specimens with quality control. This retrospective case-control study design identified 32 metabolites, including a series of N-glycated amino acids, oxidized glutathione disulfide, and nicotinamide that were differentially expressed in normal birth weight CF neonates without meconium ileus ( n = 36) as compared to gestational age/sex-matched screen-negative controls ( n = 44) after a false discovery rate adjustment ( q < 0.05). Also, 16 metabolites from DBS extracts allowed for discrimination of true CF cases from presumptive screen-positive carriers with one identified CFTR mutation and transient neonatal hypertrypsinogenemic neonates ( n = 72), who were later confirmed as unaffected due to a low sweat chloride (<29 mM) test result. Importantly, six CF-specific biomarker candidates satisfying a Bonferroni adjustment ( p < 7.25 × 10
MeSH term(s)	Biomarkers/blood ; Case-Control Studies ; Cystic Fibrosis/diagnosis ; Cystic Fibrosis/metabolism ; Dried Blood Spot Testing/methods ; Electrophoresis, Capillary ; Female ; Humans ; Infant, Newborn ; Male ; Neonatal Screening/methods ; Retrospective Studies ; Tandem Mass Spectrometry
Chemical Substances	Biomarkers
Language	English
Publishing date	2019-01-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.8b00351
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Article ; Online: Development and external validation of machine learning algorithms for postnatal gestational age estimation using clinical data and metabolomic markers.

PloS one

2023 Volume 18, Issue 3, Page(s) e0281074

Abstract: Background: Accurate estimates of gestational age (GA) at birth are important for preterm birth surveillance but can be challenging to obtain in low income countries. Our objective was to develop machine learning models to accurately estimate GA shortly ...

Abstract	Background: Accurate estimates of gestational age (GA) at birth are important for preterm birth surveillance but can be challenging to obtain in low income countries. Our objective was to develop machine learning models to accurately estimate GA shortly after birth using clinical and metabolomic data. Methods: We derived three GA estimation models using ELASTIC NET multivariable linear regression using metabolomic markers from heel-prick blood samples and clinical data from a retrospective cohort of newborns from Ontario, Canada. We conducted internal model validation in an independent cohort of Ontario newborns, and external validation in heel prick and cord blood sample data collected from newborns from prospective birth cohorts in Lusaka, Zambia and Matlab, Bangladesh. Model performance was measured by comparing model-derived estimates of GA to reference estimates from early pregnancy ultrasound. Results: Samples were collected from 311 newborns from Zambia and 1176 from Bangladesh. The best-performing model accurately estimated GA within about 6 days of ultrasound estimates in both cohorts when applied to heel prick data (MAE 0.79 weeks (95% CI 0.69, 0.90) for Zambia; 0.81 weeks (0.75, 0.86) for Bangladesh), and within about 7 days when applied to cord blood data (1.02 weeks (0.90, 1.15) for Zambia; 0.95 weeks (0.90, 0.99) for Bangladesh). Conclusions: Algorithms developed in Canada provided accurate estimates of GA when applied to external cohorts from Zambia and Bangladesh. Model performance was superior in heel prick data as compared to cord blood data.
MeSH term(s)	Infant, Newborn ; Female ; Pregnancy ; Humans ; Gestational Age ; Premature Birth ; Prospective Studies ; Retrospective Studies ; Zambia ; Algorithms ; Ankle Injuries ; Knee Injuries ; Machine Learning ; Ontario
Language	English
Publishing date	2023-03-06
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0281074
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Article ; Online: Ulcération neurotrophique après thermocoagulation du ganglion de Gasser.

Lamoureux, M / Bonnot, P / Schmidt-Guerre, A-R / Humbert, P / Algros, M-P / Meyer, C

Revue de stomatologie et de chirurgie maxillo-faciale

2011 Volume 112, Issue 6, Page(s) 372–378

Abstract: Introduction: Neurotrophic ulceration (NTU) in the trigeminal nerve sensitive area is rare. It may be caused by a lesion anywhere on the trigeminal nerve's trajectory. The diagnosis is usually clinical, but other diagnoses, particularly neoplastic, must ...

Title translation	Neurotrophic ulceration following thermocoagulation of the Gasserian ganglion.
Abstract	Introduction: Neurotrophic ulceration (NTU) in the trigeminal nerve sensitive area is rare. It may be caused by a lesion anywhere on the trigeminal nerve's trajectory. The diagnosis is usually clinical, but other diagnoses, particularly neoplastic, must be ruled out first. The physiopathology and treatment of NTU remain controversial. We report a severe case of NTU and describe the main features of this poorly documented disease. Observation: A 67-year-old female patient consulted in the dermatology department for a progressive ulceration of the nose ala and the right upper lip, having developed over the two previous years. She had undergone two thermocoagulations of the right Gasserian ganglion for facial neuralgia 3 years before. The diagnosis of NTU, initially ruled out because of biopsies suggesting verrucous carcinoma, was finally retained because of the clinical presentation and anamnesis. Treatment consisted in surgical excision and primary reconstruction using a forehead flap. The diagnosis was confirmed after histopathological examination of the surgical specimen. A recurrence was noted 2 years postoperatively, then the patient was lost to follow-up. Discussion: The physiopathology of NTUs is badly documented. The cutaneous ulcerations look like facial neoplasms but the clinical findings (unilateral and paranasal location; lesion of the trigeminal nerve; local trauma; psychological instability) suggest neurotrophic ulceration. The histopathological examination, sometimes difficult, reveals non-specific chronic inflammatory ulceration. There is no consensus on treatment. The psychological profile may be a risk factor for recurrences and must be taken into account.
MeSH term(s)	Aged ; Electrocoagulation/adverse effects ; Female ; Humans ; Nose Diseases/diagnostic imaging ; Nose Diseases/etiology ; Radiography ; Skin Ulcer/diagnostic imaging ; Skin Ulcer/etiology ; Trigeminal Ganglion/surgery ; Trigeminal Nerve/pathology ; Trigeminal Nerve Diseases/diagnostic imaging ; Trigeminal Nerve Diseases/etiology ; Trigeminal Nerve Diseases/surgery ; Ulcer/diagnostic imaging ; Ulcer/etiology
Language	French
Publishing date	2011-12
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	412547-2
ISSN	1776-257X ; 0035-1768 ; 2213-6533
ISSN (online)	1776-257X
ISSN	0035-1768 ; 2213-6533
DOI	10.1016/j.stomax.2011.01.012
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Article ; Online: Propanil in a Manitoba soil: an interactive spreadsheet model based on conventional chemical kinetics.

Gamble, Donald S / Webster, G R Barrie / Lamoureux, Marc

Journal of environmental monitoring : JEM

2012 Volume 14, Issue 4, Page(s) 1167–1173

Abstract: An interactive spreadsheet model has been created for quantitative predictions of propanil sorption and reaction in a slurried Manitoba clay soil. Based on experimental values for the numbers of empty and filled sorption sites as reactants and products, ... ...

Abstract	An interactive spreadsheet model has been created for quantitative predictions of propanil sorption and reaction in a slurried Manitoba clay soil. Based on experimental values for the numbers of empty and filled sorption sites as reactants and products, the reaction mechanism has been described with conventional chemical kinetics. The on line HPLC μ extraction method revealed labile sorption, intraparticle diffusion, and a chemical reaction. Laidler's integral rate law for second order kinetics describes the labile sorption. Desorption, intraparticle diffusion, and the chemical reaction are all described by first order kinetics. The time dependent effects of initial concentration and amount of slurried soil can be predicted for sorption, intraparticle diffusion, and the amount of reaction product. Suggested applications include storm runoff and inputs for fate and transport hydrology models.
MeSH term(s)	Herbicides/analysis ; Herbicides/chemistry ; Kinetics ; Manitoba ; Models, Chemical ; Propanil/analysis ; Propanil/chemistry ; Soil Pollutants/analysis ; Soil Pollutants/chemistry
Chemical Substances	Herbicides ; Soil Pollutants ; Propanil (709-98-8)
Language	English
Publishing date	2012-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1453583-x
ISSN	1464-0333 ; 1464-0325
ISSN (online)	1464-0333
ISSN	1464-0325
DOI	10.1039/c2em10366d
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Article ; Online: Unlocking the global health potential of dried blood spot cards.

Journal of global health

2022 Volume 12, Page(s) 3027

MeSH term(s)	Global Health ; Humans
Language	English
Publishing date	2022-07-16
Publishing country	Scotland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2741629-X
ISSN	2047-2986 ; 2047-2986
ISSN (online)	2047-2986
ISSN	2047-2986
DOI	10.7189/jogh.12.03027
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Article ; Online: Temporal Signal Pattern Recognition in Mass Spectrometry: A Method for Rapid Identification and Accurate Quantification of Biomarkers for Inborn Errors of Metabolism with Quality Assurance.

DiBattista, Alicia / McIntosh, Nathan / Lamoureux, Monica / Al-Dirbashi, Osama Y / Chakraborty, Pranesh / Britz-McKibbin, Philip

Analytical chemistry

2017 Volume 89, Issue 15, Page(s) 8112–8121

Abstract: Mass spectrometry (MS)-based metabolomic initiatives that use conventional separation techniques are limited by low sample throughput and complicated data processing that contribute to false discoveries. Herein, we introduce a new strategy for ... ...

Abstract	Mass spectrometry (MS)-based metabolomic initiatives that use conventional separation techniques are limited by low sample throughput and complicated data processing that contribute to false discoveries. Herein, we introduce a new strategy for unambiguous identification and accurate quantification of biomarkers for inborn errors of metabolism (IEM) from dried blood spots (DBS) with quality assurance. A multiplexed separation platform based on multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS) was developed to provide comparable sample throughput to flow injection analysis-tandem MS (FIA-MS/MS) but with greater selectivity as required for confirmatory testing and discovery-based metabolite profiling of volume-restricted biospecimens. Mass spectral information is encoded temporally within a separation by serial injection of three or more sample pairs, each having a unique dilution pattern, alongside a quality control (QC) that serves as a reference in every run to facilitate between-sample comparisons and/or batch correction due to system drift. Optimization of whole blood extraction conditions on DBS filter paper cut-outs was first achieved to maximize recovery of a wide range of polar metabolites from DBS extracts. An interlaboratory comparison study was also conducted using a proficiency test and retrospective neonatal DBS that demonstrated good agreement between MSI-CE-MS and validated FIA-MS/MS methods within an accredited facility. Our work demonstrated accurate identification of various IEM based on reliable measurement of a panel of primary or secondary biomarkers above an upper cutoff concentration limit for presumptive screen-positive cases without stable isotope-labeled reagents. Additionally, nontargeted metabolite profiling by MSI-CE-MS with temporal signal pattern recognition revealed new biomarkers for early detection of galactosemia, such as N-galactated amino acids, that are a novel class of pathognomonic marker due to galactose stress in affected neonates.
MeSH term(s)	Biomarkers/analysis ; Dried Blood Spot Testing ; Electrophoresis, Capillary ; Humans ; Infant, Newborn ; Metabolism, Inborn Errors/diagnosis ; Metabolism, Inborn Errors/metabolism ; Quality Control ; Signal Processing, Computer-Assisted ; Spectrometry, Mass, Electrospray Ionization/methods ; Spectrometry, Mass, Electrospray Ionization/standards
Chemical Substances	Biomarkers
Language	English
Publishing date	2017-07-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.7b01727
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Article ; Online: Longitudinal Study of

Jolicoeur, Alice P / Lemay, Marie-Laurence / Beaubien, Elyse / Bélanger, Jessy / Bergeron, Claudia / Bourque-Leblanc, Françoise / Doré, Laurie / Dupuis, Marie-Ève / Fleury, Audrey / Garneau, Josiane E / Labrie, Simon J / Labrie, Steve / Lacasse, Geneviève / Lamontagne-Drolet, Marianne / Lessard-Hurtubise, Roxanne / Martel, Bruno / Menasria, Rym / Morin-Pelchat, Rachel / Pageau, Gabrielle /

Samson, Julie E / Rousseau, Geneviève M / Tremblay, Denise M / Duquenne, Manon / Lamoureux, Maryse / Moineau, Sylvain

Applied and environmental microbiology

2023 Volume 89, Issue 5, Page(s) e0042123

Abstract: The presence of virulent phages is closely monitored during cheese manufacturing, as these bacterial viruses can significantly slow down the milk fermentation process and lead to low-quality cheeses. From 2001 to 2020, whey samples from cheddar cheese ... ...

Abstract	The presence of virulent phages is closely monitored during cheese manufacturing, as these bacterial viruses can significantly slow down the milk fermentation process and lead to low-quality cheeses. From 2001 to 2020, whey samples from cheddar cheese production in a Canadian factory were monitored for the presence of virulent phages capable of infecting proprietary strains of Lactococcus cremoris and Lactococcus lactis used in starter cultures. Phages were successfully isolated from 932 whey samples using standard plaque assays and several industrial
MeSH term(s)	Humans ; Cheese/microbiology ; Multilocus Sequence Typing ; Phylogeny ; Longitudinal Studies ; Canada ; Bacteriophages ; Lactococcus lactis/genetics ; Siphoviridae/genetics ; Multiplex Polymerase Chain Reaction
Language	English
Publishing date	2023-04-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/aem.00421-23
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