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  1. Book ; Thesis: Vergleichende Beanspruchungsanalyse nach Implantation verschiedener zementfreier Hüftendoprothesen

    Langhans, Markus C.

    1989  

    Author's details vorgelegt von Markus Christian Langhans
    Keywords Hip Prosthesis / standards ; Models, Anatomic ; Biomechanics
    Size 147 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Gießen, Univ., Diss., 1991
    HBZ-ID HT004213024
    Database Catalogue ZB MED Medicine, Health

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    92 D 2702 order for loan Magazin

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  2. Article ; Online: Combination of hydrophobicity and codon usage bias determines sorting of model K

    Engel, Anja J / Paech, Steffen / Langhans, Markus / van Etten, James L / Moroni, Anna / Thiel, Gerhard / Rauh, Oliver

    Traffic (Copenhagen, Denmark)

    2023  Volume 24, Issue 11, Page(s) 533–545

    Abstract: ... When the ... ...

    Abstract When the K
    MeSH term(s) Animals ; Codon Usage ; Proteins/metabolism ; Mitochondria/metabolism ; Protein Transport ; Endoplasmic Reticulum/metabolism ; Codon/metabolism ; Hydrophobic and Hydrophilic Interactions ; Mammals/genetics ; Mammals/metabolism
    Chemical Substances Proteins ; Codon
    Language English
    Publishing date 2023-08-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1483852-7
    ISSN 1600-0854 ; 1398-9219
    ISSN (online) 1600-0854
    ISSN 1398-9219
    DOI 10.1111/tra.12915
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5634: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Toward Fabrication of Bioactive Papers: Covalent Immobilization of Peptides and Proteins

    Liebich, Valentina J. / Avrutina, Olga / Habermann, Jan / Hillscher, Laura M. / Langhans, Markus / Meckel, Tobias / Biesalski, Markus / Kolmar, Harald

    Biomacromolecules. 2021 June 08, v. 22, no. 7

    2021  

    Abstract: Herein, we report a novel two-step method for the covalent, site-directed, and efficient immobilization of proteins on lab-made paper sheets. First, paper fibers were modified with a peptidic anchor comprising enzyme recognition motifs. Four different ... ...

    Abstract Herein, we report a novel two-step method for the covalent, site-directed, and efficient immobilization of proteins on lab-made paper sheets. First, paper fibers were modified with a peptidic anchor comprising enzyme recognition motifs. Four different conjugation strategies for peptide immobilization were evaluated with respect to reproducibility and fiber loading efficiency. After manufacturing of the peptide-preconditioned paper, oriented conjugation of the model protein tGFP containing a C-terminal recognition sequence for either sortase A or microbial transglutaminase was assessed semiquantitatively by fluorescence measurement and inspected by confocal laser scanning microscopy (CLSM). The two enzymes utilized for protein conjugation used the same oligoglycine peptide anchor, and both proved to be suitable for controlled oriented linkage of substrate proteins at physiological conditions.
    Keywords fluorescence ; paper ; peptides ; protein-glutamine gamma-glutamyltransferase ; sortase A
    Language English
    Dates of publication 2021-0608
    Size p. 2954-2962.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1526-4602
    DOI 10.1021/acs.biomac.1c00354
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Light-Regulated Transcription of a Mitochondrial-Targeted K

    Engel, Anja J / Winterstein, Laura-Marie / Kithil, Marina / Langhans, Markus / Moroni, Anna / Thiel, Gerhard

    Cells

    2020  Volume 9, Issue 11

    Abstract: The inner membranes of mitochondria contain several types of ... ...

    Abstract The inner membranes of mitochondria contain several types of K
    MeSH term(s) Humans ; Membrane Potentials/physiology ; Mitochondria/metabolism ; Potassium Channels/metabolism
    Chemical Substances Potassium Channels
    Language English
    Publishing date 2020-11-19
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells9112507
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Single-molecule detection and tracking in plants.

    Langhans, Markus / Meckel, Tobias

    Protoplasma

    2014  Volume 251, Issue 2, Page(s) 277–291

    Abstract: Combining optical properties with a limited choice of fluorophores turns single-molecule imaging in plants into a challenging task. This explains why the technique, despite its success in the field of animal cell biology, is far from being routinely ... ...

    Abstract Combining optical properties with a limited choice of fluorophores turns single-molecule imaging in plants into a challenging task. This explains why the technique, despite its success in the field of animal cell biology, is far from being routinely applied in plant cell research. The same challenges, however, also apply to the application of single-molecule microscopy to any intact tissue or multicellular 3D cell culture. As recent and upcoming progress in fluorescence microscopy will permit single-molecule detection in the context of multicellular systems, plant tissue imaging will experience a huge benefit from this progress. In this review, we address every step of a single-molecule experiment, highlight the critical aspects of each and elaborate on optimizations and developments required for improvements. We relate each step to recent achievements, which have so far been conducted exclusively on the root epidermis of Arabidopsis thaliana seedlings with inclined illumination and show examples of single-molecule measurements using different cells or illumination schemes.
    MeSH term(s) Arabidopsis/chemistry ; Arabidopsis/cytology ; Arabidopsis/ultrastructure ; Microscopy, Fluorescence/methods ; Nanotechnology/methods ; Plant Structures/ultrastructure
    Language English
    Publishing date 2014-01-03
    Publishing country Austria
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 123809-7
    ISSN 1615-6102 ; 0033-183X
    ISSN (online) 1615-6102
    ISSN 0033-183X
    DOI 10.1007/s00709-013-0601-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2366: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Single-molecule detection and tracking in plants

    Langhans, Markus / Meckel, Tobias

    Protoplasma. 2014 Mar., v. 251, no. 2

    2014  

    Abstract: Combining optical properties with a limited choice of fluorophores turns single-molecule imaging in plants into a challenging task. This explains why the technique, despite its success in the field of animal cell biology, is far from being routinely ... ...

    Abstract Combining optical properties with a limited choice of fluorophores turns single-molecule imaging in plants into a challenging task. This explains why the technique, despite its success in the field of animal cell biology, is far from being routinely applied in plant cell research. The same challenges, however, also apply to the application of single-molecule microscopy to any intact tissue or multicellular 3D cell culture. As recent and upcoming progress in fluorescence microscopy will permit single-molecule detection in the context of multicellular systems, plant tissue imaging will experience a huge benefit from this progress. In this review, we address every step of a single-molecule experiment, highlight the critical aspects of each and elaborate on optimizations and developments required for improvements. We relate each step to recent achievements, which have so far been conducted exclusively on the root epidermis of Arabidopsis thaliana seedlings with inclined illumination and show examples of single-molecule measurements using different cells or illumination schemes.
    Keywords Arabidopsis thaliana ; animals ; cell biology ; cell culture ; fluorescence microscopy ; image analysis ; lighting ; monitoring ; optical properties ; plant tissues ; seedlings
    Language English
    Dates of publication 2014-03
    Size p. 277-291.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 123809-7
    ISSN 1615-6102 ; 0033-183X
    ISSN (online) 1615-6102
    ISSN 0033-183X
    DOI 10.1007/s00709-013-0601-0
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 46/342: Show issues

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  7. Article ; Online: Toward Fabrication of Bioactive Papers: Covalent Immobilization of Peptides and Proteins.

    Liebich, Valentina J / Avrutina, Olga / Habermann, Jan / Hillscher, Laura M / Langhans, Markus / Meckel, Tobias / Biesalski, Markus / Kolmar, Harald

    Biomacromolecules

    2021  Volume 22, Issue 7, Page(s) 2954–2962

    Abstract: Herein, we report a novel two-step method for the covalent, site-directed, and efficient immobilization of proteins on lab-made paper sheets. First, paper fibers were modified with a peptidic anchor comprising enzyme recognition motifs. Four different ... ...

    Abstract Herein, we report a novel two-step method for the covalent, site-directed, and efficient immobilization of proteins on lab-made paper sheets. First, paper fibers were modified with a peptidic anchor comprising enzyme recognition motifs. Four different conjugation strategies for peptide immobilization were evaluated with respect to reproducibility and fiber loading efficiency. After manufacturing of the peptide-preconditioned paper, oriented conjugation of the model protein tGFP containing a C-terminal recognition sequence for either sortase A or microbial transglutaminase was assessed semiquantitatively by fluorescence measurement and inspected by confocal laser scanning microscopy (CLSM). The two enzymes utilized for protein conjugation used the same oligoglycine peptide anchor, and both proved to be suitable for controlled oriented linkage of substrate proteins at physiological conditions.
    MeSH term(s) Bacterial Proteins ; Peptides ; Reproducibility of Results ; Transglutaminases
    Chemical Substances Bacterial Proteins ; Peptides ; Transglutaminases (EC 2.3.2.13)
    Language English
    Publishing date 2021-06-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1526-4602
    ISSN (online) 1526-4602
    DOI 10.1021/acs.biomac.1c00354
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Reducing Unspecific Protein Adsorption in Microfluidic Papers Using Fiber-Attached Polymer Hydrogels.

    von Stockert, Alexander Ritter / Luongo, Anna / Langhans, Markus / Brandstetter, Thomas / Rühe, Jürgen / Meckel, Tobias / Biesalski, Markus

    Sensors (Basel, Switzerland)

    2021  Volume 21, Issue 19

    Abstract: Microfluidic paper combines pump-free water transport at low cost with a high degree of sustainability, as well as good availability of the paper-forming cellulosic material, thus making it an attractive candidate for point-of-care (POC) analytics and ... ...

    Abstract Microfluidic paper combines pump-free water transport at low cost with a high degree of sustainability, as well as good availability of the paper-forming cellulosic material, thus making it an attractive candidate for point-of-care (POC) analytics and diagnostics. Although a number of interesting demonstrators for such paper devices have been reported to date, a number of challenges still exist, which limit a successful transfer into marketable applications. A strong limitation in this respect is the (unspecific) adsorption of protein analytes to the paper fibers during the lateral flow assay. This interaction may significantly reduce the amount of analyte that reaches the detection zone of the microfluidic paper-based analytical device (µPAD), thereby reducing its overall sensitivity. Here, we introduce a novel approach on reducing the nonspecific adsorption of proteins to lab-made paper sheets for the use in µPADs. To this, cotton linter fibers in lab-formed additive-free paper sheets are modified with a surrounding thin hydrogel layer generated from photo-crosslinked, benzophenone functionalized copolymers based on poly-(oligo-ethylene glycol methacrylate) (POEGMA) and poly-dimethyl acrylamide (PDMAA). This, as we show in tests similar to lateral flow assays, significantly reduces unspecific binding of model proteins. Furthermore, by evaporating the transport fluid during the microfluidic run at the end of the paper strip through local heating, model proteins can almost quantitatively be accumulated in that zone. The possibility of complete, almost quantitative protein transport in a µPAD opens up new opportunities to significantly improve the signal-to-noise (S/N) ratio of paper-based lateral flow assays.
    MeSH term(s) Adsorption ; Hydrogels ; Microfluidics ; Paper ; Polymers
    Chemical Substances Hydrogels ; Polymers
    Language English
    Publishing date 2021-09-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s21196348
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  9. Article ; Online: ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells.

    Eckert, Denise / Rapp, Felicitas / Tsedeke, Ayele T / Molendowska, Jessica / Lehn, Robert / Langhans, Markus / Fournier, Claudia / Rödel, Franz / Hehlgans, Stephanie

    Cells

    2021  Volume 11, Issue 1

    Abstract: Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose-effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a ... ...

    Abstract Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose-effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0-2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.
    MeSH term(s) Carbon ; Cell Adhesion/radiation effects ; Cell Adhesion Molecules/metabolism ; Cells, Cultured ; Dose-Response Relationship, Radiation ; Endothelial Cells/cytology ; Endothelial Cells/radiation effects ; Gene Expression Regulation/radiation effects ; Humans ; Leukocytes/cytology ; Leukocytes/radiation effects ; Microvessels/cytology ; Models, Biological ; NF-E2-Related Factor 2/metabolism ; Oxidation-Reduction ; Oxidative Stress/radiation effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Reactive Oxygen Species/metabolism ; X-Rays
    Chemical Substances Cell Adhesion Molecules ; NF-E2-Related Factor 2 ; NFE2L2 protein, human ; RNA, Messenger ; Reactive Oxygen Species ; Carbon (7440-44-0)
    Language English
    Publishing date 2021-12-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11010072
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Codon Bias Can Determine Sorting of a Potassium Channel Protein.

    Engel, Anja J / Kithil, Marina / Langhans, Markus / Rauh, Oliver / Cartolano, Matea / Van Etten, James L / Moroni, Anna / Thiel, Gerhard

    Cells

    2021  Volume 10, Issue 5

    Abstract: Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, ... ...

    Abstract Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K
    MeSH term(s) Codon Usage ; Genetic Code ; Humans ; Potassium Channels/genetics ; Potassium Channels/metabolism ; Protein Biosynthesis ; Protein Transport
    Chemical Substances Potassium Channels
    Language English
    Publishing date 2021-05-07
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10051128
    Database MEDical Literature Analysis and Retrieval System OnLINE

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