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Article: [Methodical approach to the construction of the index of doctors' confidence in their activities].

Russkikh, S V / Larionov, A V / Vasiliev, M D

Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny

2023 Volume 31, Issue Special Issue 2, Page(s) 1183–1188

Abstract: Introduction: The COVID-19 pandemic has demonstrated the importance of developing a state policy to increase the attractiveness of a doctor's work and counteract professional burnout. The trust of doctors in their work determines the state of human ... ...

Abstract	Introduction: The COVID-19 pandemic has demonstrated the importance of developing a state policy to increase the attractiveness of a doctor's work and counteract professional burnout. The trust of doctors in their work determines the state of human resources in the field of healthcare. The development of an approach to the organization of monitoring the trust of doctors will allow identifying organizations at an early stage in which there is a decrease in the confidence of doctors in their activities. The purpose of the study is to develop and test a methodological approach to calculating the index of trust of doctors. Materials and methods: To construct the index, a list of statements was developed that are applicable for a comprehensive assessment of the doctor's activities. The approbation of the developed approach was carried out as part of a pilot sociological study in which 346 doctors from different regions of Russia took part. The significance of the statements used in the assessment was confirmed by binary probit regression. Results: The analysis made it possible to calculate the index of doctors' trust for the obtained sample, equal to 62,5%. The results of the study can be used to assess the attitude of doctors to their activities both at the regional level and at the level of individual organizations. The Ministry of Health of the Russian Federation can use the results obtained to develop approaches to increasing the attractiveness of the medical profession to increase the public health of Russia.
MeSH term(s)	Humans ; Pandemics ; Physicians ; Delivery of Health Care ; Trust ; Public Health
Language	Russian
Publishing date	2023-12-08
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article
ZDB-ID	1483853-9
ISSN	0869-866X
ISSN	0869-866X
DOI	10.32687/0869-866X-2023-31-s2-1183-1188
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Article: Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer.

Druzhinin, V G / Baranova, E D / Demenkov, P S / Matskova, L V / Larionov, A V

Vavilovskii zhurnal genetiki i selektsii

2024 Volume 28, Issue 2, Page(s) 204–214

Abstract: Recent studies have shown that the bacterial microbiome of the respiratory tract influences the development of lung cancer. Changes in the composition of the microbiome are observed in patients with chronic inflammatory processes. Such microbiome changes ...

Abstract	Recent studies have shown that the bacterial microbiome of the respiratory tract influences the development of lung cancer. Changes in the composition of the microbiome are observed in patients with chronic inflammatory processes. Such microbiome changes may include the occurrence of bacteria that cause oxidative stress and that are capable of causing genome damage in the cells of the host organism directly and indirectly. To date, the composition of the respiratory microbiome in patients with various histological variants of lung cancer has not been studied. In the present study, we determined the taxonomic composition of the sputum microbiome of 52 patients with squamous cell carcinoma of the lung, 52 patients with lung adenocarcinoma and 52 healthy control donors, using next-generation sequencing (NGS) on the V3-V4 region of the bacterial gene encoding 16S rRNA. The sputum microbiomes of patients with different histological types of lung cancer and controls did not show significant differences in terms of the species richness index (Shannon); however, the patients differed from the controls in terms of evenness index (Pielou). The structures of bacterial communities (beta diversity) in the adenocarcinoma and squamous cell carcinoma groups were also similar; however, when analyzed according to the matrix constructed by the Bray-Curtis method, there were differences between patients with squamous cell carcinoma and healthy subjects, but not between those with adenocarcinoma and controls. Using the LEFse method it was possible to identify an increase in the content of Bacillota (Streptococcus and Bacillus) and Actinomycetota (Rothia) in the sputum of patients with squamous cell carcinoma when compared with samples from patients with adenocarcinoma. There were no differences in the content of bacteria between the samples of patients with adenocarcinoma and the control ones. The content of representatives of the genera Streptococcus, Bacillus, Peptostreptococcus (phylum Bacillota), Prevotella, Macellibacteroides (phylum Bacteroidota), Rothia (phylum Actinomycetota) and Actinobacillus (phylum Pseudomonadota) was increased in the microbiome of sputum samples from patients with squamous cell carcinoma, compared with the control. Thus, the sputum bacterial microbiome of patients with different histological types of non-small-cell lung cancer has significant differences. Further research should be devoted to the search for microbiome biomarkers of lung cancer at the level of bacterial species using whole-genome sequencing.
Language	English
Publishing date	2024-04-27
Publishing country	Russia (Federation)
Document type	Journal Article
ISSN	2500-0462
ISSN	2500-0462
DOI	10.18699/vjgb-24-25
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Article: Assessment of the State of Economic Security in Russia Using the Example of the Unemployment Rate Indicator: Fractal Analysis Method.

Belyaev, I I / Larionov, A V / Sil'vestrov, S N

Studies on Russian economic development

2021 Volume 32, Issue 2, Page(s) 141–146

Abstract: The article proposes methodological approaches to the verification of indicators of the state of the country's economic security using the method of fractal analysis. Fractal analysis technologies make it possible to determine the nature and dynamics of ... ...

Abstract	The article proposes methodological approaches to the verification of indicators of the state of the country's economic security using the method of fractal analysis. Fractal analysis technologies make it possible to determine the nature and dynamics of changes in the indicator, to verify its values (indicative or critical), and also to reveal the rate at which these states are reached on the time horizon of statistical observations. The unemployment rate indicator is selected as an example.
Language	English
Publishing date	2021-05-06
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	2226332-9
ISSN	1531-8664 ; 1075-7007
ISSN (online)	1531-8664
ISSN	1075-7007
DOI	10.1134/S1075700721020027
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Article: Microarray-Based Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Lung Cancer Patients

Minina, V. I. / Druzhinin, V. G. / Larionov, A. V. / Baranova, E. D. / Buslaev, V. Yu / Matskova, L. V. / Bakanova, M. L.

Russian journal of genetics. 2022 July, v. 58, no. 7

2022

Abstract: Lung cancer is the most commonly occurring cancer in men worldwide. To search for new biological markers of this pathology, the transcriptome of the blood mononuclear cells from patients and healthy donors (residents of Kemerovo oblast, Russia) was ... ...

Abstract	Lung cancer is the most commonly occurring cancer in men worldwide. To search for new biological markers of this pathology, the transcriptome of the blood mononuclear cells from patients and healthy donors (residents of Kemerovo oblast, Russia) was studied using SurePrint G3 Human Gene Expression microarray technology. A total of 288 differentially expressed genes were identified, including 108 up-regulated genes and 180 down-regulated genes. Functional enrichment analysis using the WebGestalt resource and different databases (Gene Ontology, KEGG, and Reactome) indicated changes in the expression profiles of genes involved in the processes of immune response, protein synthesis, cell cycle control, and apoptosis. Analysis of protein–protein interactions using the STRING algorithm made it possible to identify functional clusters of gene products with different expression levels.
Keywords	Russia ; algorithms ; apoptosis ; cell cycle ; gene expression ; gene expression regulation ; gene ontology ; genes ; humans ; immune response ; lung neoplasms ; microarray technology ; protein synthesis ; transcriptome ; transcriptomics
Language	English
Dates of publication	2022-07
Size	p. 814-822.
Publishing place	Pleiades Publishing
Document type	Article
ZDB-ID	1195858-3
ISSN	1608-3369 ; 1022-7954
ISSN (online)	1608-3369
ISSN	1022-7954
DOI	10.1134/S1022795422070122
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Article: [The genetic diagnostics of mutations UGT1A1 in practice of modern medicine.]

Volkov, A N / Khabieva, S M / Smirnova, E Yu / Larionov, A V

Klinicheskaia laboratornaia diagnostika

2019 Volume 63, Issue 3, Page(s) 186–192

Abstract: The detection of mutations of the gene of UDF-glucuronyltransferase A1 (UGT1A1) has an important practical value. The carriers of mutant genotypes, mainly *28/*28, are characterized by a reduced function of glucuronidation and excretion of a number of ... ...

Abstract	The detection of mutations of the gene of UDF-glucuronyltransferase A1 (UGT1A1) has an important practical value. The carriers of mutant genotypes, mainly 28/28, are characterized by a reduced function of glucuronidation and excretion of a number of endogenous and exogenous toxins. A precise association of particular forms of benign hyperbilirubinemia (especially Gilbert's syndrome) with mutations in promoter and exonic areas of UGT1A1 is established. On the other hand, carriers of various genotypes of UGT1A1 differ significantly in metabolism characteristics of a number of common medications (irinotecan, belinostat, etc.), that requires a dosage of these medications considering individual genetic status of patient. The analysis of modern technical solutions for genetic diagnostics of UGT1A1 mutations is carried out. The particular attention is paid to discussion of national developments for genetic typing of UGT1A1. The conclusion is made concerning small assortment of corresponding test-systems of Russian production. In some cases, there is no data about their main analytical and diagnostic characteristics. When developing design of diagnosticums, various methodological approaches are applied that allow to potential consumers to choose depending on financial technical capabilities of laboratory, amount of implemented analyses, qualification of personnel. To support UGT1A1 research instrumentally, laboratory equipment of national manufacturers can be sufficient that would permit to organize entire analytical cycle on the basis of import substitution principle.
MeSH term(s)	Exons ; Genotype ; Glucuronosyltransferase/genetics ; Humans ; Mutation ; Polymorphism, Genetic ; Promoter Regions, Genetic ; Russia
Chemical Substances	UGT1A1 enzyme (EC 2.4.1.-) ; Glucuronosyltransferase (EC 2.4.1.17)
Language	Russian
Publishing date	2019-01-23
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	1155086-7
ISSN	0869-2084
ISSN	0869-2084
DOI	10.18821/0869-2084-2018-63-3-186-192
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Zs.MO 647: Show issues

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Article ; Online: Investigation of spin stiffness in spin-depolarized states of two-dimensional electron systems with time-resolved Kerr rotation.

Larionov, A V / Stepanets-Khussein, E / Kulik, L V / Umansky, V / Kukushkin, I V

Scientific reports

2020 Volume 10, Issue 1, Page(s) 2270

Abstract: An experimental technique based on time-resolved Kerr rotation allows a comparison of the spin stiffnesses of different spin-polarized and depolarized states in a two-dimensional electron system. With this technique, a new spin-correlated phase that has ... ...

Abstract	An experimental technique based on time-resolved Kerr rotation allows a comparison of the spin stiffnesses of different spin-polarized and depolarized states in a two-dimensional electron system. With this technique, a new spin-correlated phase that has no known analogues was discovered. The new spin-depolarized phase is characterized by high spin stiffness equal to that of a spin-polarized quantum Hall ferromagnet.
Language	English
Publishing date	2020-02-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-58658-4
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Article: Complex Approach to Evaluating Genotoxicity from Occupational Factors in Coal Mining Industry

Meyer, A. V / Tolochko, T. A / Minina, V. I / Timofeeva, A. A / Larionov, A. V

Russian journal of genetics. 2020 May, v. 56, no. 5

2020

Abstract: The level of cytogenetic damage was analyzed in coal miners (N = 116) and the control sample (N = 169) using an assay for scoring of chromosomal aberrations (CAs) and the DNA comet assay in lymphocytes, as well as the micronucleus test in buccal ... ...

Abstract	The level of cytogenetic damage was analyzed in coal miners (N = 116) and the control sample (N = 169) using an assay for scoring of chromosomal aberrations (CAs) and the DNA comet assay in lymphocytes, as well as the micronucleus test in buccal epithelial cells (MN in BEC). The group of coal miners was characterized by a statistically significantly increase in the main index values of the used test system, compared to the control group. Specifically, the level of chromosomal aberrations in the miner and control groups was 4.69 ± 0.28 and 2.13 ± 0.10%; the proportion of DNA in the comet tail was 4.33 ± 0.38 and 2.16 ± 0.24%; and the micronucleus level was 1.44 ± 0.21 and 0.23 ± 0.12‰, respectively (Р < 0.05). Moreover, in the sample of coal miners, a considerable increase in the levels of additional test indices was observed. For CAs, these parameters included the frequency of single fragments, chromatid-type aberrations, paired fragments, dicentrics without fragments, chromosome exchanges, and chromosome-type aberrations; for the DNA comet assay, these were the comet tail moment and Olive tail moment; for the MN in BEC, the frequencies of binucleated cells, cells with notched nuclei, geminate nuclei, karyorhexis, and apoptotic bodies. Ranking of the results according to major cytogenetic abnormalities made it possible to establish that, in the group of miners, the proportion of individuals with the index values above the background was 68.97% for CAs, 52.13% for the DNA comet assay, and 36.08% for the MN in BEC. The proportion of individuals with cytogenetic damage higher than the background simultaneously in three test systems was 20% of the total sample of miners. To assess the mutagenic effects from occupational factors of coal mining enterprises, it seems reasonable to use a complex of test systems (CA test, DNA comet assay, and MN in BEC).
Keywords	DNA ; apoptosis ; chromosomes ; coal ; comet assay ; epithelium ; genotoxicity ; micronucleus tests ; mutagens
Language	English
Dates of publication	2020-05
Size	p. 611-617.
Publishing place	Pleiades Publishing
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	1195858-3
ISSN	1608-3369 ; 1022-7954
ISSN (online)	1608-3369
ISSN	1022-7954
DOI	10.1134/S1022795420050105
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Article: The Length of Telomeres and the Baseline Level of Cytogenetic Damage in Leukocytes of Lung Cancer Patients

Druzhinin, V. G. / Baranova, E. D. / Volobaev, V. P. / Ivanov, V. I. / Larionov, A. V. / Minina, V. I. / Smagulova, F. / Legoff, L. / Titov, V. A. / Fucic, A.

Russian journal of genetics. 2022 Feb., v. 58, no. 1

2022

Abstract: Despite the great efforts of basic and clinical research on lung cancer (LC), the prevention and treatment of this type of cancer show still far from satisfactory indicators. The complex mechanisms of the LC biology are already recognized, but there is a ...

Abstract	Despite the great efforts of basic and clinical research on lung cancer (LC), the prevention and treatment of this type of cancer show still far from satisfactory indicators. The complex mechanisms of the LC biology are already recognized, but there is a significant gap in the knowledge about the interaction of key biomarkers in the etiology and progression of this disease. The present work is aimed at studying the relationship between the telomere length (TL) and frequencies of chromosomal aberrations (CA) and micronuclei (MN) in leukocytes of primary lung cancer patients. In 67 men with lung cancer and 77 control donors (men), the baseline frequencies of CA and MN were determined in blood leukocytes, and TL was also studied. As a result, a significant increase in TL was found in lung cancer patients compared with control donors (25.86 ± 2.13 versus 15.29 ± 1.22, respectively, p = 0.00001). In leukocytes of LC patients, a significant increase in the baseline level of structural aberrations of chromosomes, as well as micronuclei containing centromeres, was noted. Only in the sample of lung cancer patients were direct correlations of TL with the frequency of chromosomal type aberrations and with the frequency of paired fragments revealed. In the same sample, significant inverse correlations of TL with the frequency of binucleated leukocytes containing nuclear protrusions and with the frequency of cells entering apoptosis were recorded. The study first showed that an increase in TL in lung cancer patients is associated with structural chromosomal damages, but not with the incidence of aneuploidies.
Keywords	aneuploidy ; apoptosis ; biomarkers ; biomedical research ; centromeres ; etiology ; lung neoplasms ; telomeres
Language	English
Dates of publication	2022-02
Size	p. 73-84.
Publishing place	Pleiades Publishing
Document type	Article
ZDB-ID	1195858-3
ISSN	1608-3369 ; 1022-7954
ISSN (online)	1608-3369
ISSN	1022-7954
DOI	10.1134/S1022795422010021
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Article: [Ecological interpretation of higher units of steppe vegetation in southern mountains of Middle Siberia via quantitative ordination method].

Ermakov, N B / Larionov, A V / Polyakova, M A / Plugatar, Yu V

Zhurnal obshchei biologii

2016 Volume 77, Issue 4, Page(s) 293–302

Abstract: Detrended correspondent analysis is used for constructing an ecological ordination model of higher units of steppe vegetation in southern mountains of Middle Siberia. The model is based on 326 complete geobotanical descriptions and correlation analysis ... ...

Abstract	Detrended correspondent analysis is used for constructing an ecological ordination model of higher units of steppe vegetation in southern mountains of Middle Siberia. The model is based on 326 complete geobotanical descriptions and correlation analysis between the values of major axes and climatic, soil-ground, and geographical parameters. In the space of two first principal ordination axes, ecological series of steppe vegetation coenofloras are observed which are orientated along climatic factors of annual and seasonal precipitation, temperature, oceanity-continentality, and ground stoniness. A syntaxonomic interpretation of observed ecological-geographical steppe types is given, and the hierarchy of higher classification units is substantiated from the ecological point of view.
MeSH term(s)	Grassland ; Models, Biological ; Plants ; Siberia
Language	Russian
Publishing date	2016-07
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	955645-x
ISSN	0044-4596
ISSN	0044-4596
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Article ; Online: Genetic damage in lymphocytes of lung cancer patients is correlated to the composition of the respiratory tract microbiome.

Druzhinin, V G / Matskova, L V / Demenkov, P S / Baranova, E D / Volobaev, V P / Minina, V I / Larionov, A V / Titov, V A / Fucic, A

Mutagenesis

2021 Volume 36, Issue 2, Page(s) 143–153

Abstract: Recent findings indicate that the microbiome may have significant impact on the development of lung cancer by its effects on inflammation, dysbiosis or genome damage. The aim of this study was to compare the sputum microbiome of lung cancer (LC) patients ...

Abstract	Recent findings indicate that the microbiome may have significant impact on the development of lung cancer by its effects on inflammation, dysbiosis or genome damage. The aim of this study was to compare the sputum microbiome of lung cancer (LC) patients with the chromosomal aberration (CA) and micronuclei (MN) frequency in peripheral blood lymphocytes. In the study, the taxonomic composition of the sputum microbiome of 66 men with untreated LC were compared with 62 control subjects with respect to CA and MN frequency and centromere fluorescence in situ hybridisation analysis. Results showed a significant increase in CA (4.11 ± 2.48% versus 2.08 ± 1.18%) and MN (1.53 ± 0.67% versus 0.87 ± 0.49%) frequencies, respectively, in LC patients as compared to control subjects. The higher frequency of centromeric positive MN of LC patients was mainly due to aneuploidy. A significant increase in Streptococcus, Bacillus, Gemella and Haemophilus in LC patients was detected, in comparison to the control subjects while 18 bacterial genera were significantly reduced, which indicates a decrease in the beta diversity in the microbiome of LC patients. Although, the CA frequency in LC patients is significantly associated with an increased presence of the genera Bacteroides, Lachnoanaerobaculum, Porphyromonas, Mycoplasma and Fusobacterium in their sputum, and a decrease for the genus Granulicatella after application of false discovery rate correction, significance was not any more present. The decrease of MN frequency of LC patients is significantly associated with an increase in Megasphaera genera and Selenomonas bovis. In conclusion, a significant difference in beta diversity of microbiome between LC and control subjects and association between the sputum microbiome composition and genome damage of LC patients was detected, thus supporting previous studies suggesting an etiological connection between the airway microbiome and LC.
MeSH term(s)	Adult ; Aged ; Aneuploidy ; Biodiversity ; Centromere/genetics ; Chromosome Aberrations/statistics & numerical data ; DNA Damage ; DNA, Bacterial ; Dysbiosis/microbiology ; Humans ; Inflammation/microbiology ; Lung Neoplasms/microbiology ; Lymphocytes ; Male ; Microbiota ; Micronuclei, Chromosome-Defective/statistics & numerical data ; Middle Aged ; RNA, Ribosomal, 16S ; Respiratory System/microbiology ; Sputum/microbiology
Chemical Substances	DNA, Bacterial ; RNA, Ribosomal, 16S
Language	English
Publishing date	2021-01-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632903-2
ISSN	1464-3804 ; 0267-8357
ISSN (online)	1464-3804
ISSN	0267-8357
DOI	10.1093/mutage/geab004
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