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Article: Damage-induced basal epithelial cell migration modulates the spatial organization of redox signaling and sensory neuron regeneration.

Fister, Alexandra M / Horn, Adam / Lasarev, Michael / Huttenlocher, Anna

bioRxiv : the preprint server for biology

2024

Abstract: Epithelial damage leads to early reactive oxygen species (ROS) signaling, which regulates sensory neuron regeneration and tissue repair. How the initial type of tissue injury influences early damage signaling and regenerative growth of sensory axons ... ...

Abstract	Epithelial damage leads to early reactive oxygen species (ROS) signaling, which regulates sensory neuron regeneration and tissue repair. How the initial type of tissue injury influences early damage signaling and regenerative growth of sensory axons remains unclear. Previously we reported that thermal injury triggers distinct early tissue responses in larval zebrafish. Here, we found that thermal but not mechanical injury impairs sensory axon regeneration and function. Real-time imaging revealed an immediate tissue response to thermal injury characterized by the rapid Arp2/3-dependent migration of keratinocytes, which was associated with tissue-scale ROS production and sustained sensory axon damage. Isotonic treatment was sufficient to limit keratinocyte movement, spatially restrict ROS production and rescue sensory neuron function. These results suggest that early keratinocyte dynamics regulate the spatial and temporal pattern of long-term signaling in the wound microenvironment during tissue repair.
Language	English
Publishing date	2024-05-03
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.03.14.532628
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Article: Risk factors of enterococcal bacteriuria in cats: A retrospective study.

Clark, Hannah / Lasarev, Michael / Wood, Michael

The Canadian veterinary journal = La revue veterinaire canadienne

2023 Volume 64, Issue 1, Page(s) 40–44

Abstract: Objective: To determine if factors associated with urothelial damage and inflammation, including urinary catheterization, urinary obstruction, and urolithiasis are associated with the presence of enterococcal bacteriuria in cats.: Animals: Thirty-one ...

Abstract	Objective: To determine if factors associated with urothelial damage and inflammation, including urinary catheterization, urinary obstruction, and urolithiasis are associated with the presence of enterococcal bacteriuria in cats. Animals: Thirty-one cats with Procedure: A retrospective case-control study with cases and controls identified by records search for Results: Urinary catheterization, urinary obstruction, and urolithiasis were not observed more often in Conclusion: No association was identified between urinary catheterization, urolithiasis, or any other comorbidities (hyperthyroidism, chronic kidney disease) and enterococcal bacteriuria in cats. Clinical relevance: Unlike in humans and dogs, urothelial damage and inflammation caused by factors such as urinary catheterization and urolithiasis may not be the mechanism for enterococcal bacteriuria in cats.
MeSH term(s)	Animals ; Cats ; Bacteriuria/epidemiology ; Bacteriuria/veterinary ; Bacteriuria/diagnosis ; Case-Control Studies ; Cat Diseases/epidemiology ; Cat Diseases/etiology ; Enterococcus ; Escherichia coli ; Inflammation/complications ; Inflammation/veterinary ; Retrospective Studies ; Risk Factors ; Urethral Diseases/veterinary ; Urinary Tract Infections/veterinary ; Urolithiasis/epidemiology ; Urolithiasis/veterinary
Language	English
Publishing date	2023-01-01
Publishing country	Canada
Document type	Journal Article
ZDB-ID	41603-4
ISSN	0008-5286
ISSN	0008-5286
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Article ; Online: A novel factor IXa-specific enzyme-linked immunosorbent assay detects factor IXa in human plasma.

Misenheimer, Tina M / Lasarev, Michael R / Kumfer, Kraig T / Sheehan, John P / Schwartz, Bradford S

Research and practice in thrombosis and haemostasis

2024 Volume 8, Issue 1, Page(s) 102338

Abstract: Background: Factor (F)IXa activity has been detected in human plasma and may impact thrombotic risk. Current FIXa activity assays are complex and cumbersome.: Objectives: To develop a reproducible enzyme-linked immunosorbent assay (ELISA) using a ... ...

Abstract	Background: Factor (F)IXa activity has been detected in human plasma and may impact thrombotic risk. Current FIXa activity assays are complex and cumbersome. Objectives: To develop a reproducible enzyme-linked immunosorbent assay (ELISA) using a novel monoclonal antibody that detects total FIXa in human plasma. Methods: A monoclonal antibody was raised against the new N-terminus exposed upon activation of FIX to FIXa by cleavage after R226. This antibody is specific for FIXa protease and does not recognize FIX zymogen or FIXα. The antibody was used to develop a FIXa-specific ELISA capable of quantifying total FIXa (free FIXa and FIXa-antithrombin complex) in human plasma. Total FIXa quantified using the ELISA was compared to that of FIXa-antithrombin quantified using modifications of a previously described ELISA. Results: The FIXa-specific ELISA was reproducible and quantified total FIXa in human plasma. Total FIXa levels correlated with FIXa-antithrombin levels. Conclusion: A monoclonal antibody was developed that specifically detects human FIXa protease. A FIXa-specific ELISA using the new antibody is capable of reproducibly measuring total FIXa in human plasma (both free FIXa and FIXa-antithrombin). This assay should facilitate the evaluation of total FIXa levels in a variety of clinical circumstances.
Language	English
Publishing date	2024-02-05
Publishing country	United States
Document type	Journal Article
ISSN	2475-0379
ISSN (online)	2475-0379
DOI	10.1016/j.rpth.2024.102338
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Article: Decreased Intraoperative Renal Tissue Oxygenation after Cardiopulmonary Bypass Predicts Cardiac Surgery-Associated Acute Kidney Injury in Neonates.

Condit, Paige E / Gorski, Daniel P / Lasarev, Michael R / Al-Subu, Awni M / Harer, Matthew W

Children (Basel, Switzerland)

2024 Volume 11, Issue 3

Abstract: 1) Background: Near-infrared spectroscopy (NIRS) is a noninvasive tool frequently used during cardiac surgery and postoperatively in the cardiac intensive care unit to monitor regional tissue oxygen saturation. A relationship between trends of ... ...

Abstract	(1) Background: Near-infrared spectroscopy (NIRS) is a noninvasive tool frequently used during cardiac surgery and postoperatively in the cardiac intensive care unit to monitor regional tissue oxygen saturation. A relationship between trends of intraoperative renal oxygenation and the risk of developing cardiac surgery-associated acute kidney injury (AKI) post-operatively has not yet been established in the neonatal population. The objective of this study is to evaluate the relationship of cerebral and renal oxygenation during cardiopulmonary bypass with cardiac surgery-associated AKI in the first 72 h post-operation in neonates < 30 days of age. (2) Methods: A prospective cohort study at a tertiary care children's hospital was performed. Renal and cerebral oxygenation measured were collected intraoperatively from neonates < 30 days of age who underwent cardiopulmonary bypass for the correction of congenital heart disease. AKI was defined accordance with the Kidney Disease: Improving Global Outcomes criteria modified for neonates. Variables were compared between groups. (3) Results: 32 neonates with 35 cardiopulmonary bypass cases were included. AKI was diagnosed in 60% of cases. Intra-operative renal oxygenation, both on- and off-bypass, did not differ among the three AKI groups (
Language	English
Publishing date	2024-03-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2732685-8
ISSN	2227-9067
ISSN	2227-9067
DOI	10.3390/children11030315
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Article ; Online: Comparison of preoperative retrobulbar bupivacaine and postoperative subcutaneous liposome-encapsulated bupivacaine on postoperative analgesia in dogs undergoing enucleation.

Opgenorth, Taylor A / Bentley, Ellison / Smith, Lesley J / Bartholomew, Kyle J / Lasarev, Michael R

Journal of the American Veterinary Medical Association

2024 , Page(s) 1–7

Abstract: Objective: To compare the effectiveness of preoperative bupivacaine inferotemporal retrobulbar blocks to postoperative liposome-encapsulated bupivacaine (Nocita) line blocks for analgesia following enucleation.: Animals: 39 client-owned dogs (40 eyes) ...

Abstract	Objective: To compare the effectiveness of preoperative bupivacaine inferotemporal retrobulbar blocks to postoperative liposome-encapsulated bupivacaine (Nocita) line blocks for analgesia following enucleation. Animals: 39 client-owned dogs (40 eyes) presenting to the Ophthalmology Service for enucleation. Methods: Dogs were randomly assigned to receive either a preoperative inferotemporal retrobulbar block with 0.5% bupivacaine or a peri-incisional line block with liposome-encapsulated bupivacaine (Nocita) at closure. Patients underwent unilateral enucleation and were hospitalized for 24 hours after surgery. Pain scores were performed by a masked observer with the Glasgow Composite Measure Pain Scale and the University of Wisconsin Ocular Pain Scale at 0.25, 0.5, 1, 2, 4, 6, 8, and 24 hours following surgery. Intraoperative use of blood pressure and anesthetic support mediations as well as need for rescue pain control were recorded and compared between groups. Results: There was no significant difference in rescue rates between treatment groups. When comparing the use of medical intraoperative heart rate, blood pressure, or anesthetic plane support, there were no significant differences in use between groups. Clinical relevance: Use of preoperative bupivacaine retrobulbar blocks and postoperative Nocita line blocks were equally effective at postoperative pain control with similarly low complication rates.
Language	English
Publishing date	2024-02-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	390811-2
ISSN	1943-569X ; 0003-1488
ISSN (online)	1943-569X
ISSN	0003-1488
DOI	10.2460/javma.23.11.0629
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Zs.A 423: Show issues

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Article ; Online: Renal tissue oxygenation and development of AKI in preterm neonates born < 32 weeks' gestational age in the first week of age.

Condit, Paige E / Chuck, Jennifer E / Lasarev, Michael R / Chock, Valerie Y / Harer, Matthew W

Journal of perinatology : official journal of the California Perinatal Association

2024 Volume 44, Issue 3, Page(s) 434–438

Abstract: Objective: To evaluate the relationship between regional renal saturation of oxygen (RrSO: Design: Post-hoc analysis of multicenter prospectively measured neonatal RrSO: Results: One hundred nine neonates were included and 561 SCr values were ... ...

Abstract	Objective: To evaluate the relationship between regional renal saturation of oxygen (RrSO Design: Post-hoc analysis of multicenter prospectively measured neonatal RrSO Results: One hundred nine neonates were included and 561 SCr values were obtained. Eight participants developed AKI by SCr criteria. A 10-percentage point increase in mean %RrSO Conclusions: Increases in mean %RrSO
MeSH term(s)	Humans ; Infant, Newborn ; Acute Kidney Injury/etiology ; Creatinine ; Gestational Age ; Infant, Newborn, Diseases ; Kidney ; Retrospective Studies ; Multicenter Studies as Topic
Chemical Substances	Creatinine (AYI8EX34EU)
Language	English
Publishing date	2024-01-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	645021-0
ISSN	1476-5543 ; 0743-8346
ISSN (online)	1476-5543
ISSN	0743-8346
DOI	10.1038/s41372-024-01873-y
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Zs.A 2412: Show issues

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Article ; Online: Neutrophil motility is regulated by both cell intrinsic and endothelial cell ARPC1B.

Peterson, Ashley / Bennin, David / Lasarev, Michael / Chini, Julia / Beebe, David J / Huttenlocher, Anna

Journal of cell science

2024 Volume 137, Issue 3

Abstract: Neutrophil-directed motility is necessary for host defense, but its dysregulation can also cause collateral tissue damage. Actinopathies are monogenic disorders that affect the actin cytoskeleton and lead to immune dysregulation. Deficiency in ARPC1B, a ... ...

Abstract	Neutrophil-directed motility is necessary for host defense, but its dysregulation can also cause collateral tissue damage. Actinopathies are monogenic disorders that affect the actin cytoskeleton and lead to immune dysregulation. Deficiency in ARPC1B, a component of the Arp2/3 complex, results in vascular neutrophilic inflammation; however, the mechanism remains unclear. Here, we generated human induced pluripotent stem cell (iPSC)-derived neutrophils (denoted iNeutrophils) that are deficient in ARPC1B and show impaired migration and a switch from forming pseudopodia to forming elongated filopodia. We show, using a blood vessel on a chip model, that primary human neutrophils have impaired movement across an endothelium deficient in APRC1B. We also show that the combined deficiency of ARPC1B in iNeutrophils and endothelium results in further reduction in neutrophil migration. Taken together, these results suggest that ARPC1B in endothelium is sufficient to drive neutrophil behavior. Furthermore, the findings provide support for using the iPSC system to understand human neutrophil biology and model disease in a genetically tractable system.
MeSH term(s)	Humans ; Actin-Related Protein 2-3 Complex/genetics ; Cell Movement ; Cytoskeletal Proteins ; Endothelial Cells ; Endothelium ; Induced Pluripotent Stem Cells ; Neutrophils
Chemical Substances	Actin-Related Protein 2-3 Complex ; ARPC1B protein, human ; Cytoskeletal Proteins
Language	English
Publishing date	2024-02-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.261774
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Zs.A 1200: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Newborn Screening for Severe Combined Immunodeficiency: Lessons Learned from Screening and Follow-Up of the Preterm Newborn Population.

Gaviglio, Amy / Lasarev, Michael / Sheller, Ruthanne / Singh, Sikha / Baker, Mei

International journal of neonatal screening

2023 Volume 9, Issue 4

Abstract: Newborn screening (NBS) for Severe Combined Immunodeficiency (SCID) by measurement of T-cell receptor excision circles (TRECs) successfully identifies newborns with SCID and severe T-cell lymphopenia, as intended. At the same time, NBS programs face the ... ...

Abstract	Newborn screening (NBS) for Severe Combined Immunodeficiency (SCID) by measurement of T-cell receptor excision circles (TRECs) successfully identifies newborns with SCID and severe T-cell lymphopenia, as intended. At the same time, NBS programs face the challenge of false positive results, with a disproportionately high number in the premature newborn population. This study evaluates TREC values and SCID screening outcomes in premature newborns and elucidates evidence-based SCID screening practices that reduce unnecessary follow-up activities in this population. De-identified individual SCID newborn screening data and aggregate SCID screening data were obtained from seven states across the US for babies born between 2018 and 2020. Relevant statistics were performed on data pooled from these states to quantify screening performance metrics and clinical impact on various birth and gestational age categories of newborns. The data were normalized using multiples-of-the-median (MoM) values to allow for the aggregation of data across states. The aggregation of NBS data across a range of NBS programs highlighted the trajectory of TREC values over time, both between and within newborns, and provides evidence for improved SCID screening recommendations in the premature and low birth weight population.
Language	English
Publishing date	2023-12-15
Publishing country	Switzerland
Document type	Journal Article
ISSN	2409-515X
ISSN (online)	2409-515X
DOI	10.3390/ijns9040068
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Article ; Online: Exploring knowledge gaps in the understanding of peripheral and deep en face margin assessment.

Sable, Kimberly A / Rudningen, Kyle E / Lasarev, Michael R / Xu, Yaohui G

Archives of dermatological research

2023 Volume 315, Issue 9, Page(s) 2683–2687

Abstract: In 2022, National Comprehensive Cancer Network updated the phrase of "complete circumferential peripheral and deep margin assessment (CCPDMA)" to "peripheral and deep en face margin assessment (PDEMA)," which was meant to create more consistency across ... ...

Abstract	In 2022, National Comprehensive Cancer Network updated the phrase of "complete circumferential peripheral and deep margin assessment (CCPDMA)" to "peripheral and deep en face margin assessment (PDEMA)," which was meant to create more consistency across all treatment modalities and provide clarity to the meaning of total margin evaluation. The aim of this project was to investigate the interpretation of PDEMA across pertinent specialties and to identify any existing knowledge gaps in hopes of improving clinical performance of institutional practice. An electronic survey was administered to medical professionals within the divisions of dermatology and otolaryngology retrieving demographic data and assessing respondents' knowledge on tissue processing techniques and PDEMA. Of the four knowledge-based assessment questions administered, dermatology respondents answered three questions with > 80% accuracy and one question with < 65% accuracy. Otolaryngology respondents answered one question with > 80% accuracy and three with < 65% accuracy. Both groups answered the knowledge-based question evaluating the concept of "what must be true for Mohs or PDEMA to have value" with under 65% accuracy. When comparing dermatology and otolaryngology respondents, only one question which evaluated the proper methods to "achieve processing of the epidermal edge and the base of the tumor along a single plane in the lab" significantly differed between groups, with a percentage correct of 96% for dermatologists compared to 54% for otolaryngologists (p < 0.001). Results were found to be similar when resident physicians were removed from analysis. The overall percent correct for knowledge-based questions was shifted higher for dermatologists compared to otolaryngologists (p = 0.014). This trend was also redemonstrated when analyzing the data excluding residents (p = 0.053).
MeSH term(s)	Humans ; Skin Neoplasms/diagnosis ; Skin Neoplasms/pathology ; Carcinoma, Basal Cell/pathology ; Otolaryngology ; Surveys and Questionnaires
Language	English
Publishing date	2023-05-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	130131-7
ISSN	1432-069X ; 0340-3696
ISSN (online)	1432-069X
ISSN	0340-3696
DOI	10.1007/s00403-023-02642-1
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Ui VI Zs.10: Show issues

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Article ; Online: Retrospective investigation of perioperative risk factors for immediate postoperative corneal erosions in dogs undergoing phacoemulsification.

Chen, Tiffany Mei / Lasarev, Michael R / Eaton, Joshua Seth

Veterinary ophthalmology

2022 Volume 26, Issue 3, Page(s) 191–204

Abstract: Objective: To characterize the incidence, contributing risk factors, and healing characteristics of immediate postoperative corneal erosions (IPCE) in dogs undergoing routine phacoemulsification.: Animals studied: Medical records of 313 canine eyes ( ... ...

Abstract	Objective: To characterize the incidence, contributing risk factors, and healing characteristics of immediate postoperative corneal erosions (IPCE) in dogs undergoing routine phacoemulsification. Animals studied: Medical records of 313 canine eyes (159 dogs) undergoing routine phacoemulsification surgery. Procedures: Medical records of dogs undergoing planned cataract surgery at UW Veterinary Care were retrospectively reviewed. Patient-related variables including age, skull conformation, diabetes status, and cataract stage at the time of surgery were recorded. Intraoperative variables per eye were also recorded including surgical technique, surgeon expertise level, average phacoemulsification power, and phacoemulsification time. Diagnosis of IPCE ≤ 24 h after completion of surgery and time to IPCE healing were recorded where follow-up data were available. Results: Immediate postoperative corneal erosions were observed in 48/313 (15.3%) operated eyes. The presence of diabetes mellitus or brachycephalic skull conformation, preoperative Schirmer tear test (STT) value, surgical technique and surgeon experience level, phacoemulsification time, and absolute phacoemulsification time were not statistically significant risk factors for IPCE. Average phacoemulsification power was associated with IPCE (RR 1.52, p = .001). Time to IPCE healing was similar in diabetic and non-diabetic dogs (median [IQR] 8 [6-11] days and 8 [6-15] days, respectively). Diabetes mellitus, brachycephaly, and phacoemulsification parameters were not associated with IPCE healing at 7 or 14 days postoperatively. Conclusions: Higher average phacoemulsification power may be associated with the development of IPCE in canine eyes. The presence of diabetes mellitus or brachycephaly are not risk factors for the development of IPCE, nor are they factors that influence IPCE healing.
MeSH term(s)	Dogs ; Animals ; Phacoemulsification/adverse effects ; Phacoemulsification/veterinary ; Phacoemulsification/methods ; Retrospective Studies ; Diabetes Mellitus/etiology ; Diabetes Mellitus/surgery ; Diabetes Mellitus/veterinary ; Cataract/complications ; Cataract/veterinary ; Craniosynostoses/veterinary ; Postoperative Complications/veterinary ; Postoperative Complications/epidemiology ; Dog Diseases/epidemiology
Language	English
Publishing date	2022-11-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2011043-1
ISSN	1463-5224 ; 1463-5216
ISSN (online)	1463-5224
ISSN	1463-5216
DOI	10.1111/vop.13040
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