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  1. Article: Close Links between Cold Shock Proteins and Cancer.

    Toulany, Mahmoud / Lasham, Annette

    Cancers

    2023  Volume 15, Issue 9

    Abstract: Nine of the ten papers published in this Special Issue explore various aspects of the multifunctional protein Y-box binding protein-1 (YB-1) and its role in cancer [ ... ]. ...

    Abstract Nine of the ten papers published in this Special Issue explore various aspects of the multifunctional protein Y-box binding protein-1 (YB-1) and its role in cancer [...].
    Language English
    Publishing date 2023-04-23
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers15092421
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  2. Article ; Online: Combining TP53 mutation and isoform has the potential to improve clinical practice.

    Ray Das, Sankalita / Delahunt, Brett / Lasham, Annette / Li, Kunyu / Wright, Deborah / Print, Cristin / Slatter, Tania / Braithwaite, Antony / Mehta, Sunali

    Pathology

    2024  Volume 56, Issue 4, Page(s) 473–483

    Abstract: The clinical importance of assessing and combining data on TP53 mutations and isoforms is discussed in this article. It gives a succinct overview of the structural makeup and key biological roles of the isoforms. It then provides a comprehensive summary ... ...

    Abstract The clinical importance of assessing and combining data on TP53 mutations and isoforms is discussed in this article. It gives a succinct overview of the structural makeup and key biological roles of the isoforms. It then provides a comprehensive summary of the roles that p53 isoforms play in cancer development, therapy response and resistance. The review provides a summary of studies demonstrating the role of p53 isoforms as potential prognostic indicators. It further provides evidence on how the presence of TP53 mutations may affect one or more of these activities and the association of p53 isoforms with clinicopathological data in various tumour types. The review gives insight into the present diagnostic hurdles for identifying TP53 isoforms and makes recommendations to improve their evaluation. In conclusion, this review offers suggestions for enhancing the identification and integration of TP53 isoforms in conjunction with mutation data within the clinical context.
    MeSH term(s) Humans ; Protein Isoforms/genetics ; Tumor Suppressor Protein p53/genetics ; Mutation ; Neoplasms/genetics ; Neoplasms/pathology ; Prognosis
    Chemical Substances Protein Isoforms ; Tumor Suppressor Protein p53 ; TP53 protein, human
    Language English
    Publishing date 2024-03-19
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 7085-3
    ISSN 1465-3931 ; 0031-3025
    ISSN (online) 1465-3931
    ISSN 0031-3025
    DOI 10.1016/j.pathol.2024.02.003
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 723: Show issues Location:
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  3. Article: Breast Cancer Patient Prognosis Is Determined by the Interplay between

    Lasham, Annette / Knowlton, Nicholas / Mehta, Sunali Y / Braithwaite, Antony W / Print, Cristin G

    Cancers

    2021  Volume 13, Issue 7

    Abstract: ... ...

    Abstract The
    Language English
    Publishing date 2021-03-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers13071531
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  4. Article ; Online: Dynamic ctDNA Mutational Complexity in Patients with Melanoma Receiving Immunotherapy.

    Fitzgerald, Sandra / Blenkiron, Cherie / Stephens, Rosalie / Mathy, Jon A / Somers-Edgar, Tiffany / Rolfe, Gill / Martin, Richard / Jackson, Christopher / Eccles, Michael / Robb, Tamsin / Rodger, Euan / Lawrence, Ben / Guilford, Parry / Lasham, Annette / Print, Cristin G

    Molecular diagnosis & therapy

    2023  Volume 27, Issue 4, Page(s) 537–550

    Abstract: Background: Circulating tumour DNA (ctDNA) analysis promises to improve the clinical care of people with cancer, address health inequities and guide translational research. This observational cohort study used ctDNA to follow 29 patients with advanced- ... ...

    Abstract Background: Circulating tumour DNA (ctDNA) analysis promises to improve the clinical care of people with cancer, address health inequities and guide translational research. This observational cohort study used ctDNA to follow 29 patients with advanced-stage cutaneous melanoma through multiple cycles of immunotherapy.
    Method: A melanoma-specific ctDNA next-generation sequencing (NGS) panel, droplet digital polymerase chain reaction (ddPCR) and mass spectrometry analysis were used to identify ctDNA mutations in longitudinal blood plasma samples from Aotearoa New Zealand (NZ) patients receiving immunotherapy for melanoma. These technologies were used in conjunction to identify the breadth and complexity of tumour genomic information that ctDNA analysis can reliably report.
    Results: During the course of immunotherapy treatment, a high level of dynamic mutational complexity was identified in blood plasma, including multiple BRAF mutations in the same patient, clinically relevant BRAF mutations emerging through therapy and co-occurring sub-clonal BRAF and NRAS mutations. The technical validity of this ctDNA analysis was supported by high sample analysis-reanalysis concordance, as well as concordance between different ctDNA measurement technologies. In addition, we observed > 90% concordance in the detection of ctDNA when using cell-stabilising collection tubes followed by 7-day delayed processing, compared with standard EDTA blood collection protocols with rapid processing. We also found that the undetectability of ctDNA at a proportion of treatment cycles was associated with durable clinical benefit (DCB).
    Conclusion: We found that multiple ctDNA processing and analysis methods consistently identified complex longitudinal patterns of clinically relevant mutations, adding support for expanded clinical trials of this technology in a variety of oncology settings.
    MeSH term(s) Humans ; Melanoma/genetics ; Melanoma/therapy ; Skin Neoplasms/genetics ; Skin Neoplasms/therapy ; Circulating Tumor DNA/genetics ; Proto-Oncogene Proteins B-raf/genetics ; DNA, Neoplasm ; Mutation ; Immunotherapy ; Melanoma, Cutaneous Malignant
    Chemical Substances Circulating Tumor DNA ; Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; DNA, Neoplasm
    Language English
    Publishing date 2023-04-26
    Publishing country New Zealand
    Document type Observational Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2232796-4
    ISSN 1179-2000 ; 1177-1062
    ISSN (online) 1179-2000
    ISSN 1177-1062
    DOI 10.1007/s40291-023-00651-4
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5202: Show issues Location:
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  5. Article: Accessing a New Dimension in TP53 Biology: Multiplex Long Amplicon Digital PCR to Specifically Detect and Quantitate Individual

    Lasham, Annette / Tsai, Peter / Fitzgerald, Sandra J / Mehta, Sunali Y / Knowlton, Nicholas S / Braithwaite, Antony W / Print, Cristin G

    Cancers

    2020  Volume 12, Issue 3

    Abstract: ... ...

    Abstract TP53
    Language English
    Publishing date 2020-03-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers12030769
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  6. Article ; Online: Development of biodegradable PLGA nanoparticles surface engineered with hyaluronic acid for targeted delivery of paclitaxel to triple negative breast cancer cells.

    Cerqueira, Brenda Brenner S / Lasham, Annette / Shelling, Andrew N / Al-Kassas, Raida

    Materials science & engineering. C, Materials for biological applications

    2017  Volume 76, Page(s) 593–600

    Abstract: This study aimed at development of poly (lactic-co-glycolic acid) (PLGA) nanoparticles embedded with paclitaxel and coated with hyaluronic acid (HA-PTX-PLGA) to actively target the drug to a triple negative breast cancer cells. Nanoparticles were ... ...

    Abstract This study aimed at development of poly (lactic-co-glycolic acid) (PLGA) nanoparticles embedded with paclitaxel and coated with hyaluronic acid (HA-PTX-PLGA) to actively target the drug to a triple negative breast cancer cells. Nanoparticles were successfully fabricated using a modified oil-in-water emulsion method. The effect of various formulations parameters on the physicochemical properties of the nanoparticles was investigated. SEM imaging confirmed the spherical shape and nano-scale size of the nanoparticles. A sustained drug release profile was obtained and enhanced PTX cytotoxicity was observed when MDA-MB-231 cells were incubated with the HA-PTX-PLGA formulation compared to cells incubated with the non-HA coated nanoparticles. Moreover, HA-PLGA nanoparticles exhibited improved cellular uptake, based on a possible receptor mediated endocytosis due to interaction of HA with CD44 receptors when compared to non-coated PLGA nanoparticles. The non-haemolytic potential of the nanoparticles indicated the suitability of the developed formulation for intravenous administration.
    Language English
    Publishing date 2017-07-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2012160-X
    ISSN 1873-0191 ; 0928-4931
    ISSN (online) 1873-0191
    ISSN 0928-4931
    DOI 10.1016/j.msec.2017.03.121
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  7. Article ; Online: A Study of TP53 RNA Splicing Illustrates Pitfalls of RNA-seq Methodology.

    Mehta, Sunali / Tsai, Peter / Lasham, Annette / Campbell, Hamish / Reddel, Roger / Braithwaite, Antony / Print, Cristin

    Cancer research

    2016  Volume 76, Issue 24, Page(s) 7151–7159

    Abstract: TP53 undergoes multiple RNA-splicing events, resulting in at least nine mRNA transcripts encoding at least 12 functionally different protein isoforms. Antibodies specific to p53 protein isoforms have proven difficult to develop, thus researchers must ... ...

    Abstract TP53 undergoes multiple RNA-splicing events, resulting in at least nine mRNA transcripts encoding at least 12 functionally different protein isoforms. Antibodies specific to p53 protein isoforms have proven difficult to develop, thus researchers must rely on the transcript information to infer isoform abundance. In this study, we used deep RNA-seq, droplet digital PCR (ddPCR), and real-time quantitative reverse transcriptase PCR (RT-qPCR) from nine human cell lines and RNA-seq data available for tumors in The Cancer Genome Atlas to analyze TP53 splice variant expression. All three methods detected expression of the FL/40TP53α_T1 variant in most human tumors and cell lines. However, other less abundant variants were only detected with PCR-based methods. Using RNA-seq simulation analysis, we determined why RNA-seq is unable to detect less abundant TP53 transcripts and discuss the implications of these findings for the general interpretation of RNA-seq data. Cancer Res; 76(24); 7151-9. ©2016 AACR.
    MeSH term(s) Alternative Splicing/genetics ; Cell Line, Tumor ; Gene Expression Profiling/methods ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Polymerase Chain Reaction/methods ; Protein Isoforms/analysis ; Sequence Analysis, RNA/methods ; Tumor Suppressor Protein p53/analysis ; Tumor Suppressor Protein p53/genetics
    Chemical Substances Protein Isoforms ; TP53 protein, human ; Tumor Suppressor Protein p53
    Language English
    Publishing date 2016-12-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1432-1
    ISSN 1538-7445 ; 0008-5472
    ISSN (online) 1538-7445
    ISSN 0008-5472
    DOI 10.1158/0008-5472.CAN-16-1624
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    In stock of ZB MED Cologne/Königswinter

    Uh III Zs.135/5: Show issues Location:
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  8. Article ; Online: The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing

    Lasham Annette / Coppieters Natacha / Herbert Mike / Cao Helen / Reid Glen

    BMC Research Notes, Vol 4, Iss 1, p

    2011  Volume 148

    Abstract: Abstract Background The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo , mainly relating to inefficient delivery and non-specific effects of ... ...

    Abstract Abstract Background The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo , mainly relating to inefficient delivery and non-specific effects of short RNA duplexes in animal models. The latter can lead to false positive results when real-time RT-qPCR alone is used to measure target mRNA knockdown. Findings We observed that detection of an apparent siRNA-mediated knockdown in vivo was dependent on the primers used for real-time RT-qPCR measurement of the target mRNA. Two siRNAs specific for RRM1 with equivalent activity in vitro were administered to A549 xenografts via intratumoural injection. In each case, apparent knockdown of RRM1 mRNA was observed only when the primer pair used in RT-qPCR flanked the siRNA cleavage site. This false-positive result was found to result from co-purified siRNA interfering with both reverse transcription and qPCR. Conclusions Our data suggest that using primers flanking the siRNA-mediated cleavage site in RT-qPCR-based measurements of mRNA knockdown in vivo can lead to false positive results. This is particularly relevant where high concentrations of siRNA are introduced, particularly via intratumoural injection, as the siRNA may be co-purified with the RNA and interfere with downstream enzymatic steps. Based on these results, using primers flanking the siRNA target site should be avoided when measuring knockdown of target mRNA by real-time RT-qPCR.
    Keywords Medicine ; R ; Biology (General) ; QH301-705.5 ; Science (General) ; Q1-390
    Subject code 570
    Language English
    Publishing date 2011-05-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Nanoparticulate carriers: an emerging tool for breast cancer therapy.

    Tharkar, Priyanka / Madani, Asad Ullah / Lasham, Annette / Shelling, Andrew N / Al-Kassas, Raida

    Journal of drug targeting

    2015  Volume 23, Issue 2, Page(s) 97–108

    Abstract: Breast cancer is a leading cause of death for women in the world. Cancer has the potential to spread to different organs around the body, and form metastases that can even develop after surgical removal of the primary tumour. Nanotechnology offers new ... ...

    Abstract Breast cancer is a leading cause of death for women in the world. Cancer has the potential to spread to different organs around the body, and form metastases that can even develop after surgical removal of the primary tumour. Nanotechnology offers new promising strategies for the treatment of breast cancer, and has emerged as a powerful tool for fighting cancer. Nanoparticles can be fabricated to perform more than one task simultaneously, and can have a number of roles, such as acting as a therapeutic agent, drug delivery vehicle and/or tumour imaging agent. This review will focus on various forms of nanoparticles serving as potential agents for cancer therapeutics, illustrating their use in breast cancer therapies. This article also highlights the properties, current progress in the design and engineering of nanoparticles.
    MeSH term(s) Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/therapeutic use ; Breast Neoplasms/drug therapy ; Dendrimers/chemistry ; Drug Carriers/chemistry ; Female ; Ferrosoferric Oxide/chemistry ; Humans ; Liposomes ; Nanoparticles/chemistry ; Nanoshells/chemistry ; Nanotubes, Carbon/chemistry ; Quantum Dots
    Chemical Substances Antineoplastic Agents ; Dendrimers ; Drug Carriers ; Liposomes ; Nanotubes, Carbon ; Ferrosoferric Oxide (XM0M87F357)
    Language English
    Publishing date 2015-02
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1187110-6
    ISSN 1029-2330 ; 1061-186X
    ISSN (online) 1029-2330
    ISSN 1061-186X
    DOI 10.3109/1061186X.2014.958844
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4481: Show issues Location:
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  10. Article ; Online: Nanoparticle therapeutics: Technologies and methods for overcoming cancer.

    Cerqueira, Brenda Brenner S / Lasham, Annette / Shelling, Andrew N / Al-Kassas, Raida

    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

    2015  Volume 97, Issue Pt A, Page(s) 140–151

    Abstract: It is anticipated that by 2030 approximately 13 million people will die of cancer. Common cancer therapy often fails due to the development of multidrug resistance (MDR), resulting in high morbidity and poor patient prognosis. Nanotechnology seeks to use ...

    Abstract It is anticipated that by 2030 approximately 13 million people will die of cancer. Common cancer therapy often fails due to the development of multidrug resistance (MDR), resulting in high morbidity and poor patient prognosis. Nanotechnology seeks to use drug delivery vehicles of 1-100 nm in diameter, made up of several different materials to deliver anti-cancer drugs selectively to cancer cells and potentially overcome MDR. Several technologies exist for manufacturing and functionalizing nanoparticles. When functionalized appropriately, nanoparticles have been shown to overcome several mechanisms of MDR in vivo and in vitro, reduce drug side effects and represent a promising new area of anti-cancer therapy. This review discusses the fundamental concepts of enhanced permeability and retention (EPR) effect and explores the mechanisms proposed to enhance preferential "retention" in the tumour. The overall objective of this review was to enhance our understanding in the design and development of therapeutic nanoparticles for treatment of cancer.
    MeSH term(s) Animals ; Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/adverse effects ; Antineoplastic Agents/pharmacology ; Drug Delivery Systems ; Drug Design ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Humans ; Nanoparticles ; Nanotechnology/methods ; Neoplasms/drug therapy ; Neoplasms/pathology ; Particle Size
    Chemical Substances Antineoplastic Agents
    Language English
    Publishing date 2015-11
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 1065368-5
    ISSN 1873-3441 ; 0939-6411
    ISSN (online) 1873-3441
    ISSN 0939-6411
    DOI 10.1016/j.ejpb.2015.10.007
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    Zs.B 1651: Show issues Location:
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