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  1. Article ; Online: A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differencesin Fitness during Plant Infection.

    Luneau, Julien S / Noël, Laurent D / Lauber, Emmanuelle / Boulanger, Alice

    Bio-protocol

    2022  Volume 12, Issue 13, Page(s) e3776

    Abstract: Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences ... ...

    Abstract Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences that would not otherwise be detectable. In the frame of host-pathogens interactions, they can be very useful to study the contribution of individual bacterial genes to bacterial fitness and lead to the identification of new adaptive traits. Here, we describe how to perform such competition assays by taking the example of the model phytopathogenic bacterium
    Language English
    Publishing date 2022-07-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.3776
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  2. Article: Arabidopsis thaliana

    Marzorati, Francesca / Rossi, Rossana / Bernardo, Letizia / Mauri, Pierluigi / Silvestre, Dario Di / Lauber, Emmanuelle / Noël, Laurent D / Murgia, Irene / Morandini, Piero

    Molecular plant-microbe interactions : MPMI

    2023  Volume 36, Issue 11, Page(s) 737–748

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas simiae
    MeSH term(s) Arabidopsis/metabolism ; Proteome/metabolism ; Proteomics ; Plant Roots/microbiology ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Soil ; Gene Expression Regulation, Plant
    Chemical Substances Proteome ; Arabidopsis Proteins ; Soil
    Language English
    Publishing date 2023-11-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-05-23-0071-R
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  3. Article ; Online: Bacterial host adaptation through sequence and structural variations of a single type III effector gene.

    Lauber, Emmanuelle / González-Fuente, Manuel / Escouboué, Maxime / Vicédo, Céline / Luneau, Julien S / Pouzet, Cécile / Jauneau, Alain / Gris, Carine / Zhang, Zhi-Min / Pichereaux, Carole / Carrère, Sébastien / Deslandes, Laurent / Noël, Laurent D

    iScience

    2024  Volume 27, Issue 3, Page(s) 109224

    Abstract: Molecular mechanisms underlying quantitative variations of pathogenicity remain elusive. Here, we identified ... ...

    Abstract Molecular mechanisms underlying quantitative variations of pathogenicity remain elusive. Here, we identified the
    Language English
    Publishing date 2024-02-15
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2024.109224
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  4. Article ; Online: Arabidopsis thaliana Early Foliar Proteome Response to Root Exposure to the Rhizobacterium Pseudomonas simiae WCS417

    Marzorati, Francesca / Rossi, Rossana / Bernardo, Letizia / Mauri, Pierluigi / Silvestre, Dario Di / Lauber, Emmanuelle / Noël, Laurent D. / Murgia, Irene / Morandini, Piero

    Molecular Plant-Microbe Interactions. 2023 Nov., v. 36, no. 11 p.737-748

    2023  

    Abstract: Pseudomonas simiae WCS417 is a plant growth–promoting rhizobacterium that improves plant health and development. In this study, we investigate the early leaf responses of Arabidopsis thaliana to WCS417 exposure and the possible involvement of formate ... ...

    Abstract Pseudomonas simiae WCS417 is a plant growth–promoting rhizobacterium that improves plant health and development. In this study, we investigate the early leaf responses of Arabidopsis thaliana to WCS417 exposure and the possible involvement of formate dehydrogenase (FDH) in such responses. In vitro–grown A. thaliana seedlings expressing an FDH::GUS reporter show a significant increase in FDH promoter activity in their roots and shoots after 7 days of indirect exposure (without contact) to WCS417. After root exposure to WCS417, the leaves of FDH::GUS plants grown in the soil also show an increased FDH promoter activity in hydathodes. To elucidate early foliar responses to WCS417 as well as FDH involvement, the roots of A. thaliana wild-type Col and atfdh1-5 knock-out mutant plants grown in soil were exposed to WCS417, and proteins from rosette leaves were subjected to proteomic analysis. The results reveal that chloroplasts, in particular several components of the photosystems PSI and PSII, as well as members of the glutathione S-transferase family, are among the early targets of the metabolic changes induced by WCS417. Taken together, the alterations in the foliar proteome, as observed in the atfdh1-5 mutant, especially after exposure to WCS417 and involving stress-responsive genes, suggest that FDH is a node in the early events triggered by the interactions between A. thaliana and the rhizobacterium WCS417. Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
    Keywords Arabidopsis thaliana ; Pseudomonas ; Rhizobium ; chloroplasts ; formate dehydrogenase ; glutathione transferase ; indirect contact ; knockout mutants ; leaves ; photosystem II ; plant growth-promoting rhizobacteria ; plant health ; proteome ; proteomics ; soil ; formate dehydrogenase (FDH) ; glutathione S-transferase (GST) ; hydathodes ; Pseudomonas simiae WCS417 ; rhizobacterium
    Language English
    Dates of publication 2023-11
    Size p. 737-748.
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-05-23-0071-R
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    In stock of ZB MED Bonn / Germany

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  5. Article ; Online: From effectors to effectomes: Are functional studies of individual effectors enough to decipher plant pathogen infectious strategies?

    Arroyo-Velez, Noe / González-Fuente, Manuel / Peeters, Nemo / Lauber, Emmanuelle / Noël, Laurent D

    PLoS pathogens

    2020  Volume 16, Issue 12, Page(s) e1009059

    MeSH term(s) Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/physiology ; Plant Diseases/genetics ; Plant Diseases/immunology ; Plants/immunology
    Language English
    Publishing date 2020-12-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1009059
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  6. Article ; Online: The Same against Many: AtCML8, a Ca

    Zhu, Xiaoyang / Mazard, Julie / Robe, Eugénie / Pignoly, Sarah / Aguilar, Marielle / San Clemente, Hélène / Lauber, Emmanuelle / Berthomé, Richard / Galaud, Jean-Philippe

    International journal of molecular sciences

    2021  Volume 22, Issue 19

    Abstract: Calcium signals are crucial for the activation and coordination of signaling cascades leading to the establishment of plant defense mechanisms. Here, we studied the contribution of CML8, an Arabidopsis calmodulin-like protein in response ... ...

    Abstract Calcium signals are crucial for the activation and coordination of signaling cascades leading to the establishment of plant defense mechanisms. Here, we studied the contribution of CML8, an Arabidopsis calmodulin-like protein in response to
    MeSH term(s) Arabidopsis/immunology ; Arabidopsis/metabolism ; Arabidopsis/microbiology ; Arabidopsis/physiology ; Calcium/metabolism ; Disease Resistance ; Gene Expression Regulation, Plant ; Phytophthora ; Plant Diseases ; Ralstonia solanacearum ; Signal Transduction ; Xanthomonas campestris
    Chemical Substances Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-09-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms221910469
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  7. Article: Cruciferous Weed Isolates of

    Dubrow, Zoë E / Carpenter, Sara C D / Carter, Morgan E / Grinage, Ayress / Gris, Carine / Lauber, Emmanuelle / Butchachas, Jules / Jacobs, Jonathan M / Smart, Christine D / Tancos, Matthew A / Noël, Laurent D / Bogdanove, Adam J

    Molecular plant-microbe interactions : MPMI

    2022  Volume 35, Issue 9, Page(s) 791–802

    Abstract: Pathovars ... ...

    Abstract Pathovars of
    MeSH term(s) Genomics ; Organ Specificity ; Protein Sorting Signals ; Xanthomonas/genetics ; Xanthomonas campestris/genetics
    Chemical Substances Protein Sorting Signals
    Language English
    Publishing date 2022-09-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-01-22-0024-R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection

    Luneau, Julien S. / Baudin, Maël / Quiroz Monnens, Thomas / Carrère, Sébastien / Bouchez, Olivier / Jardinaud, Marie‐Françoise / Gris, Carine / François, Jonas / Ray, Jayashree / Torralba, Babil / Arlat, Matthieu / Lewis, Jennifer D. / Lauber, Emmanuelle / Deutschbauer, Adam M. / Noël, Laurent D. / Boulanger, Alice

    The new phytologist. 2022 Oct., v. 236, no. 1

    2022  

    Abstract: Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. ... ...

    Abstract Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded‐transposon insertion site sequencing (RB‐TnSeq) to perform a genome‐wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high‐throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant‐associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c‐di‐GMP‐mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
    Keywords Brassica oleracea ; Xanthomonas campestris pv. campestris ; cauliflower ; food production ; host plants ; metabolism ; pathogens ; phenotype ; sap ; virulence ; xylem
    Language English
    Dates of publication 2022-10
    Size p. 235-248.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 208885-x
    ISSN 1469-8137 ; 0028-646X
    ISSN (online) 1469-8137
    ISSN 0028-646X
    DOI 10.1111/nph.18313
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  9. Article: Cruciferous Weed Isolates of Xanthomonas campestris Yield Insight into Pathovar Genomic Relationships and Genetic Determinants of Host and Tissue Specificity

    Dubrow, Zoë E. / Carpenter, Sara C. D. / Carter, Morgan E. / Grinage, Ayress / Gris, Carine / Lauber, Emmanuelle / Butchachas, Jules / Jacobs, Jonathan M. / Smart, Christine D. / Tancos, Matthew A. / Noël, Laurent D. / Bogdanove, Adam J.

    Molecular plant-microbe interactions. 2022 Sept., v. 35, no. 9

    2022  

    Abstract: Pathovars of Xanthomonas campestris cause distinct diseases on different brassicaceous hosts. The genomic relationships among pathovars as well as the genetic determinants of host range and tissue specificity remain poorly understood despite decades of ... ...

    Abstract Pathovars of Xanthomonas campestris cause distinct diseases on different brassicaceous hosts. The genomic relationships among pathovars as well as the genetic determinants of host range and tissue specificity remain poorly understood despite decades of research. Here, leveraging advances in multiplexed long-read technology, we fully sequenced the genomes of a collection of X. campestris strains isolated from cruciferous crops and weeds in New York and California as well as strains from global collections, to investigate pathovar relationships and candidate genes for host- and tissue-specificity. Pathogenicity assays and genomic comparisons across this collection and publicly available X. campestris genomes revealed a correlation between pathovar and genomic relatedness and provide support for X. campestris pv. barbareae, the validity of which had been questioned. Linking strain host range with type III effector repertoires identified AvrAC (also ‘XopAC’) as a candidate host-range determinant, preventing infection of Matthiola incana, and this was confirmed experimentally. Furthermore, the presence of a copy of the cellobiosidase gene cbsA with coding sequence for a signal peptide was found to correlate with the ability to infect vascular tissues, in agreement with a previous study of diverse Xanthomonas species; however, heterologous expression in strains lacking the gene gave mixed results, indicating that factors in addition to cbsA influence tissue specificity of X. campestris pathovars. Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.
    Keywords Matthiola incana ; Xanthomonas campestris ; genes ; genomics ; heterologous gene expression ; host range ; pathogenicity ; pathovars ; signal peptide ; weeds ; California ; New York
    Language English
    Dates of publication 2022-09
    Size p. 791-802.
    Publishing place The American Phytopathological Society
    Document type Article
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-01-22-0024-R
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  10. Article: Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages

    Luneau, Julien S. / Cerutti, Aude / Roux, Brice / Carrère, Sébastien / Jardinaud, Marie‐Françoise / Gaillac, Antoine / Gris, Carine / Lauber, Emmanuelle / Berthomé, Richard / Arlat, Matthieu / Boulanger, Alice / Noël, Laurent D.

    Molecular plant pathology. 2022 Feb., v. 23, no. 2

    2022  

    Abstract: Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To ... ...

    Abstract Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological and virulence strategies deployed by Xcc during early stages of infection, the transcriptomic profile of Xcc was analysed 3 days after entry into cauliflower hydathodes. Despite the absence of visible plant tissue alterations and despite a biotrophic lifestyle, 18% of Xcc genes were differentially expressed, including a striking repression of chemotaxis and motility functions. The Xcc full repertoire of virulence factors had not yet been activated but the expression of the HrpG regulon composed of 95 genes, including genes coding for the type III secretion machinery important for suppression of plant immunity, was induced. The expression of genes involved in metabolic adaptations such as catabolism of plant compounds, transport functions, sulphur and phosphate metabolism was upregulated while limited stress responses were observed 3 days postinfection. We confirmed experimentally that high‐affinity phosphate transport is needed for bacterial fitness inside hydathodes. This analysis provides information about the nutritional and stress status of bacteria during the early biotrophic infection stages and helps to decipher the adaptive strategy of Xcc to the hydathode environment.
    Keywords Brassicaceae ; Xanthomonas campestris pv. campestris ; catabolism ; cauliflower ; chemotaxis ; immunity ; leaves ; lifestyle ; pathogens ; phosphates ; plant pathology ; plant tissues ; regulon ; secretion ; sulfur ; transcriptome ; transcriptomics ; virulence
    Language English
    Dates of publication 2022-02
    Size p. 159-174.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2020755-4
    ISSN 1364-3703 ; 1464-6722
    ISSN (online) 1364-3703
    ISSN 1464-6722
    DOI 10.1111/mpp.13117
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