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  1. Article ; Online: Detection and expression of SapS, a class C nonspecific acid phosphatase with O-phospho-Ltyrosine- phosphatase activity, in Staphylococcus aureus isolates from patients with chronic osteomyelitis

    Carlos Martínez-Canseco / Rebecca E. Franco-Bourland / Norma González-Huerta / Marco Antonio Paredes-Espinosa / Silvia Giono-Cerezo / Laura Sánchez-Chapul / Rogelio Paniagua-Pérez / René Valdez-Mijares / Cecilia Hernández-Flores

    Biomédica: revista del Instituto Nacional de Salud, Vol 43, Iss 2, Pp 200-

    2023  Volume 212

    Abstract: Introduction. The identity of Staphylococcus aureus virulence factors involved in chronic osteomyelitis remains unresolved. SapS is a class C non-specific acid phosphatase and a well-known virulence factor that has been identified in S. aureus strain 154 ...

    Abstract Introduction. The identity of Staphylococcus aureus virulence factors involved in chronic osteomyelitis remains unresolved. SapS is a class C non-specific acid phosphatase and a well-known virulence factor that has been identified in S. aureus strain 154 but in protein extracts from rotting vegetables. Objective. To identify the SapS gene and characterize the activity of SapS from S. aureus strains: 12 isolates from bone infected samples of patients treated for chronic osteomyelitis and 49 from a database with in silico analysis of complete bacterial genomes. Materials and methods. The SapS gene was isolated and sequenced from 12 S. aureus clinical isolates and two reference strains; 49 S. aureus strains and 11 coagulase-negative staphylococci were tested using in silico PCR. Culture media semi-purified protein extracts from the clinical strains were assayed for phosphatase activity with p-nitro-phenylphosphate, O-phospho-L-tyrosine, O-phospho-L-serine, and OphosphoL-threonine in conjunction with various phosphatase inhibitors. Results. SapS was detected in the clinical and in-silico S. aureus strains, but not in the in silico coagulase-negative staphylococci strains. Sec-type I lipoprotein-type N-terminal signal peptide sequences; secreted proteins, and aspartate bipartite catalytic domains coding sequences were found in the SapS nucleotide and amino acid sequence analysis. SapS dephosphorylated with p-nitro-phenyl-phosphate and ophosphoLtyrosine were selectively resistant to tartrate and fluoride, but sensitive to vanadate and molybdate. Conclusion. SapS gene was found in the genome of the clinical isolates and the in silico Staphylococcus aureus strains. SapS shares biochemical similarities with known virulent bacterial, such as protein tyrosine phosphatases, suggesting it may be a virulence factor in chronic osteomyelitis.
    Keywords staphylococcus aureus ; virulence factors ; osteomyelitis ; Medicine ; R ; Arctic medicine. Tropical medicine ; RC955-962
    Subject code 610
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Instituto Nacional de Salud
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Differential gene expression of ABCG2, SLC22A12, IL-1β, and ALPK1 in peripheral blood leukocytes of primary gout patients with hyperuricemia and their comorbidities: a case-control study.

    Natsuko, Paniagua-Díaz / Laura, Sanchez-Chapul / Denise, Clavijo-Cornejo / Lucio, Ventura-Ríos / Carlos, Aguilar-Salinas / Fausto, Sanchez-Muñoz / Ambar, López-Macay

    European journal of medical research

    2022  Volume 27, Issue 1, Page(s) 62

    Abstract: Background: The ABCG2, SLC22A12, and ALPK1 genes have been strongly associated with dysfunction of urate metabolism in patients with gout, but it is unknown how these transporters are expressed in patients with acute or chronic gout. Our objectives were ...

    Abstract Background: The ABCG2, SLC22A12, and ALPK1 genes have been strongly associated with dysfunction of urate metabolism in patients with gout, but it is unknown how these transporters are expressed in patients with acute or chronic gout. Our objectives were to: (a) analyze the gene expression of urate transporters and of inflammation genes in peripheral blood from gout patients and controls; (b) determine whether the metabolic profile of gout patients can influence the gene expression profile and the expression of urate transporters, ABCG2 and SLC22A12, and inflammation molecules, ALPK1 and IL-1β, in peripheral blood leukocytes from gout patients; (c) compare them with their metabolic profile and the gene expression of people without gout and without hyperuricemia.
    Methods: A total of 36 chronic and acute patients and 52 controls were recruited, and ABCG2, SLC22A12, IL-1β, and ALPK1 gene expression was evaluated by quantitative real-time PCR. Correlations of gene expression with clinical and laboratory parameters of patients were also analyzed.
    Results: IL-1β was significantly increased in peripheral blood mononuclear cells (PBMCs) of patients compared with their polymorphonuclear leukocytes white blood cells (PMNLs, p < 0.05). A significant increase in ABCG2 and IL-1β was found in PMNLs from patients compared to controls (p < 0.05). Correlations of gene expression in patients were found with levels of serum uric acid (sUA), serum creatinine, C-reactive protein (CRP), triglycerides, body mass index (BMI), kidney disease, hypertension, and metabolic syndrome.
    Conclusions: Our data suggest that leukocytes of patients respond to the presence of hyperuricemia and comorbidities, expressing ABCG2 and IL-1β genes differentially compared to normouricemic and nondisease states. Hyperuricemia, dyslipidemia, and obesity probably stimulate the differential gene expression of peripheral blood leukocytes (neutrophils and monocytes), even in an asymptomatic state.
    MeSH term(s) ATP Binding Cassette Transporter, Subfamily G, Member 2/genetics ; Case-Control Studies ; Gene Expression ; Gout/genetics ; Humans ; Hyperuricemia/genetics ; Inflammation/genetics ; Leukocytes, Mononuclear ; Neoplasm Proteins/genetics ; Neoplasm Proteins/metabolism ; Organic Anion Transporters/genetics ; Organic Cation Transport Proteins/genetics ; Uric Acid/metabolism
    Chemical Substances ABCG2 protein, human ; ATP Binding Cassette Transporter, Subfamily G, Member 2 ; Neoplasm Proteins ; Organic Anion Transporters ; Organic Cation Transport Proteins ; SLC22A12 protein, human ; Uric Acid (268B43MJ25)
    Language English
    Publishing date 2022-05-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 1329381-3
    ISSN 2047-783X ; 0949-2321
    ISSN (online) 2047-783X
    ISSN 0949-2321
    DOI 10.1186/s40001-022-00684-1
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  3. Article ; Online: Genetic analysis of muscular dystrophies

    Rosa Elena Escobar-Cedillo / Luz López-Hernández / Antonio Miranda-Duarte / María Dolores Curiel-Leal / Andrea Suarez-Ocón / Laura Sánchez-Chapul / Alexandra Berenice Luna-Angulo / Guillermina Ávila-Ramírez / Julia Angélica López-Hernández / Benjamín Gómez-Díaz

    Folia Neuropathologica, Vol 59, Iss 3, Pp 276-

    our experience in Mexico

    2021  Volume 283

    Abstract: Muscular dystrophies are a group of well-defined genetic disorders characterized by the variable distribution of muscle wasting and progressive weakness. The diagnosis and treatment of these diseases remain challenging due to genetic heterogeneity and ... ...

    Abstract Muscular dystrophies are a group of well-defined genetic disorders characterized by the variable distribution of muscle wasting and progressive weakness. The diagnosis and treatment of these diseases remain challenging due to genetic heterogeneity and clinical overlapping. Herein, we describe our 10 years’ experience with the diagnosis and management of muscular dystrophy patients. In total, 169 patients were screened for pathogenic variants in eleven genes linked to frequent muscular dystrophies using MLPA and NGS sequencing panels. Most frequent muscular dystrophies found in the Mexican population were dystrophinopathies, dysferlinopathies and calpainopathies. Novel variants were found in genes: DMD, CAPN3, DYSF, and FKRP. For Duchenne muscular dystrophy, improvements in early diagnosis and prolonged ambulation were achieved, on the contrary, for limb-girdle muscular dystrophies and congenital muscular dystrophies, uncomplimentary follow-up and lack of detection strategies were observed. For most common muscular dystrophies, improvements in diagnosis and management have been achieved in the last 10 years, due to a collaborative effort done nationwide.
    Keywords muscular dystrophy ; dmd ; limb-girdle ; pompe ; mexico ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Termedia Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Impact of body composition on physical fitness components in the Mexican Navy

    Laura Sánchez-Chapul / Jesús F. Valencia-León / Mario Acevedo-Mora / Erika Estrada-Camarena / José U. Baños-Vázquez / Egner Hernández-Zamudio / José. A Rangel-Sánchez / Israel Gutiérrez-García / Gabriela Martínez-Nava / Liliana Téllez-Cárdenas / Diego Mirabent-Amor / José G. Franco-Sánchez / Luis A. Bonilla-Arcuate / Alexandra Luna-Angulo / Ámbar López-Macay

    Cogent Medicine, Vol 7, Iss

    Is overweight an issue?

    2020  Volume 1

    Abstract: Overweight and inadequate physical fitness are critical variables that impact directly on the health, wellbeing of service members and military readiness, characteristics required to overcome dangerous situations during sea rescues and in the first line ... ...

    Abstract Overweight and inadequate physical fitness are critical variables that impact directly on the health, wellbeing of service members and military readiness, characteristics required to overcome dangerous situations during sea rescues and in the first line of combat in the Mexican Navy. Here we determined the impact of body composition (BC) on physical fitness components (PFC) [cardiorespiratory fitness (CRF) and muscle strength-resistance (MSR)] of 110 marines, rescue swimmers, and divers of the Mexican Navy classified as overweight. We found significant differences in medians of BC and PFC among groups (p < 0.001). The WHO and military BMI showed 59 (54%) and 16 (15%) individuals as overweight, respectively. All groups showed CRF adequate for their age, but only 26 (51%) and 14 (24%) classified as normal and overweight (WHO), respectively, had adequate CFR (METs ˃12) (p = 0.003) for physical demands of military tasks. BMI in overweight at expenses of high BFP, without cardiovascular risk represents an operational task advantage that will lead to reach the balance between physical fitness, health, and military readiness.
    Keywords overweigh ; body mass index ; cardiorespiratory fitness ; body composition ; military ; Medicine ; R
    Subject code 796
    Language English
    Publishing date 2020-01-01T00:00:00Z
    Publisher Taylor & Francis Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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