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  1. Article ; Online: UPF1-like helicase grip on nucleic acids dictates processivity

    Joanne Kanaan / Saurabh Raj / Laurence Decourty / Cosmin Saveanu / Vincent Croquette / Hervé Le Hir

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 9

    Abstract: UPF1 is a highly processive helicase that plays an essential role in nonsense-mediated mRNA decay. Here the authors use single molecule binding assays to establish a functionally important relationship between helicase grip to nucleic acids, binding ... ...

    Abstract UPF1 is a highly processive helicase that plays an essential role in nonsense-mediated mRNA decay. Here the authors use single molecule binding assays to establish a functionally important relationship between helicase grip to nucleic acids, binding lifetime and the duration of translocation.
    Keywords Science ; Q
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: UPF1-like helicase grip on nucleic acids dictates processivity

    Joanne Kanaan / Saurabh Raj / Laurence Decourty / Cosmin Saveanu / Vincent Croquette / Hervé Le Hir

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 9

    Abstract: UPF1 is a highly processive helicase that plays an essential role in nonsense-mediated mRNA decay. Here the authors use single molecule binding assays to establish a functionally important relationship between helicase grip to nucleic acids, binding ... ...

    Abstract UPF1 is a highly processive helicase that plays an essential role in nonsense-mediated mRNA decay. Here the authors use single molecule binding assays to establish a functionally important relationship between helicase grip to nucleic acids, binding lifetime and the duration of translocation.
    Keywords Science ; Q
    Language English
    Publishing date 2018-09-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity

    Benoît Bragantini / Christophe Charron / Maxime Bourguet / Arnaud Paul / Decebal Tiotiu / Benjamin Rothé / Hélène Marty / Guillaume Terral / Steve Hessmann / Laurence Decourty / Marie-Eve Chagot / Jean-Marc Strub / Séverine Massenet / Edouard Bertrand / Marc Quinternet / Cosmin Saveanu / Sarah Cianférani / Stéphane Labialle / Xavier Manival /
    Bruno Charpentier

    Nature Communications, Vol 12, Iss 1, Pp 1-

    2021  Volume 17

    Abstract: Biogenesis of small nucleolar RNAs ribonucleoproteins (snoRNPs) requires dedicated assembly machinery. Here, the authors show that a subset of snoRNP assembly factors interacts, genetically or directly, with factors modulating chromatin architecture, ... ...

    Abstract Biogenesis of small nucleolar RNAs ribonucleoproteins (snoRNPs) requires dedicated assembly machinery. Here, the authors show that a subset of snoRNP assembly factors interacts, genetically or directly, with factors modulating chromatin architecture, suggesting a link between ribosome formation and chromatin functions.
    Keywords Science ; Q
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  4. Article ; Online: Long Open Reading Frame Transcripts Escape Nonsense-Mediated mRNA Decay in Yeast

    Laurence Decourty / Antonia Doyen / Christophe Malabat / Emmanuel Frachon / Delphine Rispal / Bertrand Séraphin / Frank Feuerbach / Alain Jacquier / Cosmin Saveanu

    Cell Reports, Vol 6, Iss 4, Pp 593-

    2014  Volume 598

    Abstract: Nonsense-mediated mRNA decay (NMD) destabilizes eukaryotic transcripts with long 3′ UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and open ... ...

    Abstract Nonsense-mediated mRNA decay (NMD) destabilizes eukaryotic transcripts with long 3′ UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and open reading frame (ORF) regions and with the same long, destabilizing 3′ UTR. We developed a barcode-based DNA microarray strategy to compare the levels of each reporter mRNA in strains with or without active NMD. The size of the coding region had a significant negative effect on NMD efficiency. This effect was not specific to the tested 3′ UTR because two other different NMD reporters became less sensitive to NMD when ORF length was increased. Inefficient NMD was not due to a lack of association of Upf1 to long ORF transcripts. In conclusion, in addition to a long 3′ UTR, short translation length is an important feature of NMD substrates in yeast.
    Keywords Biology (General) ; QH301-705.5
    Subject code 570
    Language English
    Publishing date 2014-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Sodium selenide toxicity is mediated by O2-dependent DNA breaks.

    Gérald Peyroche / Cosmin Saveanu / Marc Dauplais / Myriam Lazard / François Beuneu / Laurence Decourty / Christophe Malabat / Alain Jacquier / Sylvain Blanquet / Pierre Plateau

    PLoS ONE, Vol 7, Iss 5, p e

    2012  Volume 36343

    Abstract: Hydrogen selenide is a recurrent metabolite of selenium compounds. However, few experiments studied the direct link between this toxic agent and cell death. To address this question, we first screened a systematic collection of Saccharomyces cerevisiae ... ...

    Abstract Hydrogen selenide is a recurrent metabolite of selenium compounds. However, few experiments studied the direct link between this toxic agent and cell death. To address this question, we first screened a systematic collection of Saccharomyces cerevisiae haploid knockout strains for sensitivity to sodium selenide, a donor for hydrogen selenide (H(2)Se/HSe(-/)Se(2-)). Among the genes whose deletion caused hypersensitivity, homologous recombination and DNA damage checkpoint genes were over-represented, suggesting that DNA double-strand breaks are a dominant cause of hydrogen selenide toxicity. Consistent with this hypothesis, treatment of S. cerevisiae cells with sodium selenide triggered G2/M checkpoint activation and induced in vivo chromosome fragmentation. In vitro, sodium selenide directly induced DNA phosphodiester-bond breaks via an O(2)-dependent reaction. The reaction was inhibited by mannitol, a hydroxyl radical quencher, but not by superoxide dismutase or catalase, strongly suggesting the involvement of hydroxyl radicals and ruling out participations of superoxide anions or hydrogen peroxide. The (•)OH signature could indeed be detected by electron spin resonance upon exposure of a solution of sodium selenide to O(2). Finally we showed that, in vivo, toxicity strictly depended on the presence of O(2). Therefore, by combining genome-wide and biochemical approaches, we demonstrated that, in yeast cells, hydrogen selenide induces toxic DNA breaks through an O(2)-dependent radical-based mechanism.
    Keywords Medicine ; R ; Science ; Q
    Subject code 500
    Language English
    Publishing date 2012-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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