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  1. Article: Surveillance strategies following curative resection and non-operative approach of rectal cancer: How and how long? Review of current recommendations.

    Lauretta, Andrea / Montori, Giulia / Guerrini, Gian Piero

    World journal of gastrointestinal surgery

    2023  Volume 15, Issue 2, Page(s) 177–192

    Abstract: Different follow-up strategies are available for patients with rectal cancer following curative treatment. A combination of biochemical testing and imaging investigation, associated with physical examination are commonly used. However, there is currently ...

    Abstract Different follow-up strategies are available for patients with rectal cancer following curative treatment. A combination of biochemical testing and imaging investigation, associated with physical examination are commonly used. However, there is currently no consensus about the types of tests to perform, the timing of the testing, and even the need for follow-up at all has been questioned. The aim of this study was to review the evidence of the impact of different follow-up tests and programs in patients with non-metastatic disease after definitive treatment of the primary. A literature review was performed of studies published on MEDLINE, EMBASE, the Cochrane Library and Web of Science up to November 2022. Current published guidelines from the most authoritative specialty societies were also reviewed. According to the follow-up strategies available, the office visit is not efficient but represents the only way to maintain direct contact with the patient and is recommended by all authoritative specialty societies. In colorectal cancer surveillance, carcinoembryonic antigen represents the only established tumor marker. Abdominal and chest computed tomography scan is recommended considering that the liver and lungs are the most common sites of recurrence. Since local relapse in rectal cancer is higher than in colon cancer, endoscopic surveillance is mandatory. Different follow-up regimens have been published but randomized comparisons and meta-analyses do not allow to determine whether intensive or less intensive follow-up had any significant influence on survival and recurrence detection rate. The available data do not allow the drawing of final conclusions on the ideal surveillance methods and the frequency with which they should be applied. It is very useful and urgent for clinicians to identify a cost-effective strategy that allows early identification of recurrence with a special focus for high-risk patients and patients undergoing a "watch and wait" approach.
    Language English
    Publishing date 2023-02-21
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2573700-4
    ISSN 1948-9366
    ISSN 1948-9366
    DOI 10.4240/wjgs.v15.i2.177
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Atacama Clear for Complex 3D Imaging of Organs.

    de Silva, Neranjan / Lacko, Lauretta A / Jamies, Edgar A / Evans, Todd / Hurtado, Romulo

    bioRxiv : the preprint server for biology

    2024  

    Abstract: 3D reconstructive imaging is a powerful strategy to interrogate the global architecture of tissues. We developed Atacama Clear (ATC), a novel method that increases 3D imaging signal-to-noise ratios (SNRs) while simultaneously increasing the capacity of ... ...

    Abstract 3D reconstructive imaging is a powerful strategy to interrogate the global architecture of tissues. We developed Atacama Clear (ATC), a novel method that increases 3D imaging signal-to-noise ratios (SNRs) while simultaneously increasing the capacity of tissue to be cleared. ATC potentiated the clearing capacity of all tested chemical reagents currently used for optical clearing by an average of 68%, and more than doubled SNRs. This increased imaging efficacy enabled multiplex interrogation of tough fibrous tissue and specimens that naturally exhibit high levels of background noise, including the heart, kidney, and human biopsies. Indeed, ATC facilitated visualization of previously undocumented adjacent nephron segments that exhibit notoriously high autofluorescence, elements of the cardiac conduction system, and the distinct human glomerular tissue layers, at single cell resolution. Moreover, ATC was validated to be compatible with fluorescent reporter proteins in murine, zebrafish, and 3D stem cell model systems. These data establish ATC for 3D imaging studies of challenging tissue types.
    Language English
    Publishing date 2024-01-23
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.01.22.576689
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Human pluripotent-stem-cell-derived organoids for drug discovery and evaluation.

    Vandana, J Jeya / Manrique, Cassandra / Lacko, Lauretta A / Chen, Shuibing

    Cell stem cell

    2023  Volume 30, Issue 5, Page(s) 571–591

    Abstract: Human pluripotent stem cells (hPSCs) and three-dimensional organoids have ushered in a new era for disease modeling and drug discovery. Over the past decade, significant progress has been in deriving functional organoids from hPSCs, which have been ... ...

    Abstract Human pluripotent stem cells (hPSCs) and three-dimensional organoids have ushered in a new era for disease modeling and drug discovery. Over the past decade, significant progress has been in deriving functional organoids from hPSCs, which have been applied to recapitulate disease phenotypes. In addition, these advancements have extended the application of hPSCs and organoids for drug screening and clinical-trial safety evaluations. This review provides an overview of the achievements and challenges in using hPSC-derived organoids to conduct relevant high-throughput, high-contentscreens and drug evaluation. These studies have greatly enhanced our knowledge and toolbox for precision medicine.
    MeSH term(s) Humans ; Pluripotent Stem Cells ; Drug Discovery ; Drug Evaluation, Preclinical/methods ; Organoids
    Language English
    Publishing date 2023-05-06
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2375354-7
    ISSN 1875-9777 ; 1934-5909
    ISSN (online) 1875-9777
    ISSN 1934-5909
    DOI 10.1016/j.stem.2023.04.011
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Druggable targets and therapeutic development for COVID-19.

    Duan, Xiaohua / Lacko, Lauretta A / Chen, Shuibing

    Frontiers in chemistry

    2022  Volume 10, Page(s) 963701

    Abstract: Coronavirus disease (COVID-19), which is caused by SARS-CoV-2, is the biggest challenge to the global public health and economy in recent years. Until now, only limited therapeutic regimens have been available for COVID-19 patients, sparking ... ...

    Abstract Coronavirus disease (COVID-19), which is caused by SARS-CoV-2, is the biggest challenge to the global public health and economy in recent years. Until now, only limited therapeutic regimens have been available for COVID-19 patients, sparking unprecedented efforts to study coronavirus biology. The genome of SARS-CoV-2 encodes 16 non-structural, four structural, and nine accessory proteins, which mediate the viral life cycle, including viral entry, RNA replication and transcription, virion assembly and release. These processes depend on the interactions between viral polypeptides and host proteins, both of which could be potential therapeutic targets for COVID-19. Here, we will discuss the potential medicinal value of essential proteins of SARS-CoV-2 and key host factors. We summarize the most updated therapeutic interventions for COVID-19 patients, including those approved clinically or in clinical trials.
    Language English
    Publishing date 2022-10-05
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2711776-5
    ISSN 2296-2646
    ISSN 2296-2646
    DOI 10.3389/fchem.2022.963701
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Expanding the precision oncology toolkit with micro-organospheres for early cancer diagnosis.

    Vandana, J Jeya / Lacko, Lauretta A / Chen, Shuibing

    Cell stem cell

    2022  Volume 29, Issue 6, Page(s) 873–875

    Abstract: Using an automatic microfluidics droplet platform, Ding et al. successfully replicated the tumor micro-environment by generating micro-organospheres, which were then used to predict the response to anti-tumor drugs. These miniature models could be ... ...

    Abstract Using an automatic microfluidics droplet platform, Ding et al. successfully replicated the tumor micro-environment by generating micro-organospheres, which were then used to predict the response to anti-tumor drugs. These miniature models could be obtained within an extremely short time frame of 14 days, amplifying their role in facilitating cancer treatment decisions.
    MeSH term(s) Humans ; Microfluidics ; Neoplasms/diagnosis ; Precision Medicine ; Tumor Microenvironment
    Language English
    Publishing date 2022-05-31
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 2375354-7
    ISSN 1875-9777 ; 1934-5909
    ISSN (online) 1875-9777
    ISSN 1934-5909
    DOI 10.1016/j.stem.2022.05.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Commentary: The effect of Medicaid adult vision coverage on the likelihood of appropriate correction of distance vision: Evidence from the National Health and Nutrition Examination Survey.

    Muir, Lauretta A

    Social science & medicine (1982)

    2016  Volume 150, Page(s) 256–257

    MeSH term(s) Accommodation, Ocular ; Aged ; Humans ; Insurance Coverage/standards ; Medicaid/economics ; Middle Aged ; Nutrition Surveys ; United States ; Vision Disorders/economics ; Vision Disorders/therapy
    Language English
    Publishing date 2016-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 4766-1
    ISSN 1873-5347 ; 0037-7856 ; 0277-9536
    ISSN (online) 1873-5347
    ISSN 0037-7856 ; 0277-9536
    DOI 10.1016/j.socscimed.2015.12.039
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Phenotypic technologies in stem cell biology.

    Vandana, J Jeya / Lacko, Lauretta A / Chen, Shuibing

    Cell chemical biology

    2021  Volume 28, Issue 3, Page(s) 257–270

    Abstract: The high-throughput phenotypic screen (HTPS) has become an emerging technology to discover synthetic small molecules that regulate stem cell fates. Here, we review the application of HTPS to identify small molecules controlling stem cell renewal, ... ...

    Abstract The high-throughput phenotypic screen (HTPS) has become an emerging technology to discover synthetic small molecules that regulate stem cell fates. Here, we review the application of HTPS to identify small molecules controlling stem cell renewal, reprogramming, differentiation, and lineage conversion. Moreover, we discuss the use of HTPS to discover small molecules/polymers mimicking the stem cell extracellular niche. Furthermore, HTPSs have been applied on whole-animal models to identify small molecules regulating stem cell renewal or differentiation in vivo. Finally, we discuss the examples of the utilization of HTPS in stem cell-based disease modeling, as well as in the discovery of novel drug candidates for cancer, diabetes, and infectious diseases. Overall, HTPSs have provided many powerful tools for the stem cell field, which not only facilitate the generation of functional cells/tissues for replacement therapy, disease modeling, and drug screening, but also help dissect molecular mechanisms regulating physiological and pathological processes.
    MeSH term(s) Cell Biology ; Cell Differentiation ; High-Throughput Screening Assays ; Humans ; Phenotype ; Stem Cells/cytology
    Language English
    Publishing date 2021-03-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ISSN 2451-9448
    ISSN (online) 2451-9448
    DOI 10.1016/j.chembiol.2021.02.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Human organoid models to study SARS-CoV-2 infection.

    Han, Yuling / Yang, Liuliu / Lacko, Lauretta A / Chen, Shuibing

    Nature methods

    2022  Volume 19, Issue 4, Page(s) 418–428

    Abstract: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the deadliest pandemics in history. SARS-CoV-2 not only infects the respiratory tract, but also causes damage to many organs. Organoids, ... ...

    Abstract Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is one of the deadliest pandemics in history. SARS-CoV-2 not only infects the respiratory tract, but also causes damage to many organs. Organoids, which can self-renew and recapitulate the various physiology of different organs, serve as powerful platforms to model COVID-19. In this Perspective, we overview the current effort to apply both human pluripotent stem cell-derived organoids and adult organoids to study SARS-CoV-2 tropism, host response and immune cell-mediated host damage, and perform drug discovery and vaccine development. We summarize the technologies used in organoid-based COVID-19 research, discuss the remaining challenges and provide future perspectives in the application of organoid models to study SARS-CoV-2 and future emerging viruses.
    MeSH term(s) Adult ; COVID-19 ; Humans ; Organoids ; Pandemics ; Pluripotent Stem Cells ; SARS-CoV-2
    Language English
    Publishing date 2022-04-08
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/s41592-022-01453-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Een dentale oorzaak van orbitale cellulitis.

    van Lieshout, Birgit P / Bittermann, G K P Koen / Ringens, Peter J / Vaassen, Lauretta A A

    Nederlands tijdschrift voor geneeskunde

    2023  Volume 167

    Abstract: Background: Orbital cellulitis is a potentially life-threatening condition. Compression of the optical nerve can cause total or partial loss of vision. Early diagnosis is crucial to prevent complications. In case of a unilateral sinusitis as cause of a ... ...

    Title translation Odontogenic infection as cause of orbital cellulitis.
    Abstract Background: Orbital cellulitis is a potentially life-threatening condition. Compression of the optical nerve can cause total or partial loss of vision. Early diagnosis is crucial to prevent complications. In case of a unilateral sinusitis as cause of a unilateral orbital cellulitis complete clinical and dental examination combined with imaging are essential in diagnostics.
    Case description: A 53-year-old man presented with left eye movement impairment, intermittent diplopia and moderate swelling of the left lower eyelid. His diagnosis was post septal orbital cellulitis and despite administration of oral antibiotics no clinical improvement was observed. Orbital imaging by CT could not exclude a dental cause of his unilateral maxillary sinusitis. He was referred to the department of oral and maxillofacial surgery where clinical examination showed a dental cause. After removal of two decayed upper molars a complete recovery was accomplished.
    Conclusion: Odontogenic causes for unilateral orbital cellulitis should always be considered in diagnostics in adults. Clinical presentation and dental examination combined with adequate imaging can confirm the diagnosis.
    MeSH term(s) Male ; Adult ; Humans ; Middle Aged ; Orbital Cellulitis/diagnosis ; Orbital Cellulitis/etiology ; Sinusitis/complications ; Diplopia ; Anti-Bacterial Agents/therapeutic use ; Physical Examination/adverse effects ; Cellulitis/diagnosis ; Cellulitis/etiology
    Chemical Substances Anti-Bacterial Agents
    Language Dutch
    Publishing date 2023-04-17
    Publishing country Netherlands
    Document type Case Reports ; English Abstract ; Journal Article
    ZDB-ID 82073-8
    ISSN 1876-8784 ; 0028-2162
    ISSN (online) 1876-8784
    ISSN 0028-2162
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Organoid-based chemical approach to dissect the mechanism controlling cellular dynamics.

    Lacko, Lauretta A / Chen, Shuibing

    Journal of molecular cell biology

    2019  

    Language English
    Publishing date 2019-10-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2500949-7
    ISSN 1759-4685 ; 1759-4685
    ISSN (online) 1759-4685
    ISSN 1759-4685
    DOI 10.1093/jmcb/mjz100
    Database MEDical Literature Analysis and Retrieval System OnLINE

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