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  1. AU="Lautenschläger, Franziska"
  2. AU="Shah, Prasanna"
  3. AU="Stine Byberg"
  4. AU="Castro Fernandes, Ana Cristina"
  5. AU="le Comte, Marianne"
  6. AU="Datta, Karuna"
  7. AU="Kang, Zhuo"
  8. AU="Bloomquist, Jeffrey R"
  9. AU="Madas, Sapna"
  10. AU="Pickerodt, P. A."
  11. AU="Samtleben, D F E"
  12. AU="Cantile, Monica"
  13. AU="Acosta Gómez, Anyela"
  14. AU="Sarah Keefe"
  15. AU="Rossman, Jeremy S"
  16. AU="Zhou, Jiajun"
  17. AU="Arnoldini, Markus"
  18. AU="Pratt, Andy C."
  19. AU="Silva, Jéssica Coutinho"
  20. AU=Li Qing
  21. AU="Anita Matić"
  22. AU="Mazzocchi, Giampiero"

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  1. Artikel ; Online: Extracellular vimentin: Battle between the devil and the angel.

    Thalla, Divyendu Goud / Lautenschläger, Franziska

    Current opinion in cell biology

    2023  Band 85, Seite(n) 102265

    Abstract: Vimentin, an intracellular cytoskeletal protein, can be secreted by various cells in response to conditions such as injury, stress, senescence, and cancer. Once vimentin is secreted outside of the cell, it is called extracellular vimentin. This ... ...

    Abstract Vimentin, an intracellular cytoskeletal protein, can be secreted by various cells in response to conditions such as injury, stress, senescence, and cancer. Once vimentin is secreted outside of the cell, it is called extracellular vimentin. This extracellular vimentin is significantly involved in pathological conditions, particularly in the areas of viral infection, cancer, immune response, and wound healing. The effects of extracellular vimentin can be either positive or negative, for example it can enhance axonal repair but also mediates SARS-CoV-2 infection. In this review, we categorize the functional implications of extracellular vimentin based on its localization outside the cell. Specifically, we classify extracellular vimentin into two distinct forms: surface vimentin, which remains bound to the cell surface, and secreted vimentin, which refers to the free form that is completely released outside the cell. Overall, extracellular vimentin has a dual nature that encompasses both beneficial and detrimental effects on the functionality of cells, organs and whole organisms. Here, we summarize its effects in viral infection, cancer, immune response and wound healing. We find that surface vimentin is often associated with negative consequences, whereas secreted vimentin manifests predominantly with positive influences. We found that the observed effects of extracellular vimentin strongly depend on the specific circumstances under which its expression occurs in cells.
    Mesh-Begriff(e) Humans ; Axons/metabolism ; Intermediate Filaments/metabolism ; Neoplasms ; Vimentin/metabolism ; Virus Diseases ; Wound Healing ; Animals
    Chemische Substanzen Vimentin
    Sprache Englisch
    Erscheinungsdatum 2023-10-29
    Erscheinungsland England
    Dokumenttyp Journal Article ; Review
    ZDB-ID 1026381-0
    ISSN 1879-0410 ; 0955-0674
    ISSN (online) 1879-0410
    ISSN 0955-0674
    DOI 10.1016/j.ceb.2023.102265
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  2. Artikel: A low-cost alternative method of generating fibronectin micropatterned lines for cellular applications.

    Becher, Johanna Elisabeth / Lautenschläger, Franziska / Thalla, Divyendu Goud

    MethodsX

    2023  Band 10, Seite(n) 102240

    Abstract: The cellular microenvironment contributes to the architecture, differentiation, polarity, mechanics and functions of the cell [1]. Spatial confinement of cells using micropatterning techniques allows to alter and regulate the cellular microenvironment ... ...

    Abstract The cellular microenvironment contributes to the architecture, differentiation, polarity, mechanics and functions of the cell [1]. Spatial confinement of cells using micropatterning techniques allows to alter and regulate the cellular microenvironment for a better understanding of cellular mechanisms [2]. However, commercially available micropatterned consumables such as coverslips, dishes, plates etc. are expensive. These methods are complex and based on deep UV patterning [3,4]. In this study, we establish a low-cost method for effective micropatterning using Polydimethylsiloxane (PDMS) chips.•We demonstrate this method by generating fibronectin-coated micropatterned lines (width, 5 µm) on a glass bottom dish.•As a proof of concept, we culture macrophages on these lines. We additionally show that this method allows to determine the cellular polarity by measuring the position of the nucleus within a cell on a micropatterned line.
    Sprache Englisch
    Erscheinungsdatum 2023-06-01
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2023.102240
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Distinct speed and direction memories of migrating dendritic cells diversify their search strategies.

    Shaebani, M Reza / Piel, Matthieu / Lautenschläger, Franziska

    Biophysical journal

    2022  Band 121, Heft 21, Seite(n) 4099–4108

    Abstract: Migrating cells exhibit various motility patterns, resulting from different migration mechanisms, cell properties, or cell-environment interactions. The complexity of cell dynamics is reflected, e.g., in the diversity of the observed forms of velocity ... ...

    Abstract Migrating cells exhibit various motility patterns, resulting from different migration mechanisms, cell properties, or cell-environment interactions. The complexity of cell dynamics is reflected, e.g., in the diversity of the observed forms of velocity autocorrelation function-which has been widely served as a measure of diffusivity and spreading. By analyzing the dynamics of migrating dendritic cells in vitro, we disentangle the contributions of direction θ and speed v to the velocity autocorrelation. We find that the ability of cells to maintain their speed or direction of motion is unequal, reflected in different temporal decays of speed and direction autocorrelation functions, AC
    Mesh-Begriff(e) Motion ; Dendritic Cells
    Sprache Englisch
    Erscheinungsdatum 2022-09-30
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218078-9
    ISSN 1542-0086 ; 0006-3495
    ISSN (online) 1542-0086
    ISSN 0006-3495
    DOI 10.1016/j.bpj.2022.09.033
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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 235: Hefte anzeigen Standort:
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  4. Buch ; Online ; Dissertation / Habilitation: Secretion Of Vimentin and its influence on cellular functionality

    Thalla, Divyendu Goud [Verfasser] / Lautenschläger, Franziska [Akademischer Betreuer]

    2023  

    Verfasserangabe Divyendu Goud Thalla ; Betreuer: Franziska Lautenschläger
    Schlagwörter Medizin, Gesundheit ; Medicine, Health
    Thema/Rubrik (Code) sg610
    Sprache Englisch
    Verlag Saarländische Universitäts- und Landesbibliothek
    Erscheinungsort Saarbrücken
    Dokumenttyp Buch ; Online ; Dissertation / Habilitation
    Datenquelle Digitale Dissertationen im Internet

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  5. Artikel: Actin stabilization in cell migration.

    Baltes, Carsten / Thalla, Divyendu Goud / Kazmaier, Uli / Lautenschläger, Franziska

    Frontiers in cell and developmental biology

    2022  Band 10, Seite(n) 931880

    Abstract: Actin is a cytoskeletal filament involved in numerous biological tasks, such as providing cells a shape or generating and transmitting forces. Particularly important for these tasks is the ability of actin to grow and shrink. To study the role of actin ... ...

    Abstract Actin is a cytoskeletal filament involved in numerous biological tasks, such as providing cells a shape or generating and transmitting forces. Particularly important for these tasks is the ability of actin to grow and shrink. To study the role of actin in living cells this dynamic needs to be targeted. In the past, such alterations were performed by destabilizing actin. In contrast, we used the natural compound miuraenamide A in living retinal pigmented epithelial (RPE-1) cells to stabilize actin filaments and show that it decreases actin filament dynamics and elongates filament length. Cells treated with miuraenamide A increased their adhesive area and express more focal adhesion sites. These alterations result in a lower migration speed as well as a shift of nuclear position. We therefore postulate that miuraenamide A is a promising new tool to stabilize actin polymerization and study cellular behavior such as migration.
    Sprache Englisch
    Erscheinungsdatum 2022-08-11
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2022.931880
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  6. Artikel: Characterization of immune cell migration using microfabrication.

    Vesperini, Doriane / Montalvo, Galia / Qu, Bin / Lautenschläger, Franziska

    Biophysical reviews

    2021  Band 13, Heft 2, Seite(n) 185–202

    Abstract: The immune system provides our defense against pathogens and aberrant cells, including tumorigenic and infected cells. Motility is one of the fundamental characteristics that enable immune cells to find invading pathogens, control tissue damage, and ... ...

    Abstract The immune system provides our defense against pathogens and aberrant cells, including tumorigenic and infected cells. Motility is one of the fundamental characteristics that enable immune cells to find invading pathogens, control tissue damage, and eliminate primary developing tumors, even in the absence of external treatments. These processes are termed "immune surveillance." Migration disorders of immune cells are related to autoimmune diseases, chronic inflammation, and tumor evasion. It is therefore essential to characterize immune cell motility in different physiologically and pathologically relevant scenarios to understand the regulatory mechanisms of functionality of immune responses. This review is focused on immune cell migration, to define the underlying mechanisms and the corresponding investigative approaches. We highlight the challenges that immune cells encounter in vivo, and the microfabrication methods to mimic particular aspects of their microenvironment. We discuss the advantages and disadvantages of the proposed tools, and provide information on how to access them. Furthermore, we summarize the directional cues that regulate individual immune cell migration, and discuss the behavior of immune cells in a complex environment composed of multiple directional cues.
    Sprache Englisch
    Erscheinungsdatum 2021-02-11
    Erscheinungsland Germany
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2486483-3
    ISSN 1867-2469 ; 1867-2450
    ISSN (online) 1867-2469
    ISSN 1867-2450
    DOI 10.1007/s12551-021-00787-9
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  7. Buch ; Online: Distinct Speed and Direction Memories of Migrating Dendritic Cells Diversify Their Search Strategies

    Shaebani, M. Reza / Piel, Matthieu / Lautenschläger, Franziska

    2022  

    Abstract: Migrating cells exhibit various motility patterns, resulting from different migration mechanisms, cell properties, or cell-environment interactions. The complexity of cell dynamics is reflected, e.g., in the diversity of the observed forms of velocity ... ...

    Abstract Migrating cells exhibit various motility patterns, resulting from different migration mechanisms, cell properties, or cell-environment interactions. The complexity of cell dynamics is reflected, e.g., in the diversity of the observed forms of velocity autocorrelation function -- that has been widely served as a measure of diffusivity and spreading -- . By analyzing the dynamics of migrating dendritic cells in vitro, we disentangle the contributions of direction and speed to the velocity autocorrelation. We find that the ability of cells to maintain their speed or direction of motion is unequal, reflected in power-law decays of speed and direction autocorrelation functions with different exponents. The larger power-law exponent of the speed autocorrelation function indicates that the cells lose their speed memory considerably faster than the direction memory. Using numerical simulations, we investigate the influence of speed and direction memories as well as the direction-speed cross-correlation on the search time of a persistent random walker to find a randomly located target in confinement. Although the direction memory and direction-speed coupling play the major roles, we find that the speed autocorrelation can be also tuned to minimize the search time. Adopting an optimal speed memory can reduce the search time even up to 10% compared to uncorrelated spontaneous speeds. Our results suggest that migrating cells can improve their search efficiency, especially in crowded environments, through the directional or speed persistence or the speed-direction correlation.

    Comment: 10 pages, 9 figures
    Schlagwörter Physics - Biological Physics ; Condensed Matter - Soft Condensed Matter ; Condensed Matter - Statistical Mechanics
    Thema/Rubrik (Code) 612
    Erscheinungsdatum 2022-05-21
    Erscheinungsland us
    Dokumenttyp Buch ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  8. Artikel ; Online: Role of Extracellular Vimentin in Cancer-Cell Functionality and Its Influence on Cell Monolayer Permeability Changes Induced by SARS-CoV-2 Receptor Binding Domain.

    Thalla, Divyendu Goud / Jung, Philipp / Bischoff, Markus / Lautenschläger, Franziska

    International journal of molecular sciences

    2021  Band 22, Heft 14

    Abstract: The cytoskeletal protein vimentin is secreted under various physiological conditions. Extracellular vimentin exists primarily in two forms: attached to the outer cell surface and secreted into the extracellular space. While surface vimentin is involved ... ...

    Abstract The cytoskeletal protein vimentin is secreted under various physiological conditions. Extracellular vimentin exists primarily in two forms: attached to the outer cell surface and secreted into the extracellular space. While surface vimentin is involved in processes such as viral infections and cancer progression, secreted vimentin modulates inflammation through reduction of neutrophil infiltration, promotes bacterial elimination in activated macrophages, and supports axonal growth in astrocytes through activation of the IGF-1 receptor. This receptor is overexpressed in cancer cells, and its activation pathway has significant roles in general cellular functions. In this study, we investigated the functional role of extracellular vimentin in non-tumorigenic (MCF-10a) and cancer (MCF-7) cells through the evaluation of its effects on cell migration, proliferation, adhesion, and monolayer permeability. Upon treatment with extracellular recombinant vimentin, MCF-7 cells showed increased migration, proliferation, and adhesion, compared to MCF-10a cells. Further, MCF-7 monolayers showed reduced permeability, compared to MCF-10a monolayers. It has been shown that the receptor binding domain of SARS-CoV-2 spike protein can alter blood-brain barrier integrity. Surface vimentin also acts as a co-receptor between the SARS-CoV-2 spike protein and the cell-surface angiotensin-converting enzyme 2 receptor. Therefore, we also investigated the permeability of MCF-10a and MCF-7 monolayers upon treatment with extracellular recombinant vimentin, and its modulation of the SARS-CoV-2 receptor binding domain. These findings show that binding of extracellular recombinant vimentin to the cell surface enhances the permeability of both MCF-10a and MCF-7 monolayers. However, with SARS-CoV-2 receptor binding domain addition, this effect is lost with MCF-7 monolayers, as the extracellular vimentin binds directly to the viral domain. This defines an influence of extracellular vimentin in SARS-CoV-2 infections.
    Mesh-Begriff(e) Breast/metabolism ; Breast/pathology ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Membrane Permeability ; Cells, Cultured ; Extracellular Matrix/metabolism ; Female ; Humans ; Protein Domains ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/metabolism ; Vimentin/genetics ; Vimentin/metabolism
    Chemische Substanzen Spike Glycoprotein, Coronavirus ; Vimentin ; spike protein, SARS-CoV-2
    Sprache Englisch
    Erscheinungsdatum 2021-07-12
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22147469
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  9. Artikel: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis.

    Thalla, Divyendu Goud / Rajwar, Ashish Chand / Laurent, Annalena Maria / Becher, Johanna Elisabeth / Kainka, Lucina / Lautenschläger, Franziska

    Frontiers in cell and developmental biology

    2022  Band 10, Seite(n) 891281

    Abstract: Macrophages have a vital role in the immune system through elimination of cell debris and microorganisms by phagocytosis. The activation of macrophages by tumour necrosis factor-α induces expression of extracellular cell-surface vimentin and promotes ... ...

    Abstract Macrophages have a vital role in the immune system through elimination of cell debris and microorganisms by phagocytosis. The activation of macrophages by tumour necrosis factor-α induces expression of extracellular cell-surface vimentin and promotes release of this vimentin into the extracellular environment. Vimentin is a cytoskeletal protein that is primarily located in the cytoplasm of cells. However, under circumstances like injury, stress, senescence and activation, vimentin can be expressed on the extracellular cell surface, or it can be released into the extracellular space. The characteristics of this extracellular vimentin, and its implications for the functional role of macrophages and the mechanism of secretion remain unclear. Here, we demonstrate that vimentin is released mainly from the back of macrophage-like cells. This polarisation is strongly enhanced upon macrophage activation. One-dimensional patterned lines showed that extracellular cell-surface vimentin is localised primarily at the back of activated macrophage-like cells. Through two-dimensional migration and phagocytosis assays, we show that this extracellular vimentin enhances migration and phagocytosis of macrophage-like cells. We further show that this extracellular vimentin forms agglomerates on the cell surface, in contrast to its intracellular filamentous form, and that it is released into the extracellular space in the form of small fragments. Taken together, we provide new insights into the release of extracellular cell-surface vimentin and its implications for macrophage functionality.
    Sprache Englisch
    Erscheinungsdatum 2022-07-18
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2022.891281
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  10. Artikel: Vimentin Intermediate Filament Rings Deform the Nucleus During the First Steps of Adhesion.

    Terriac, Emmanuel / Schütz, Susanne / Lautenschläger, Franziska

    Frontiers in cell and developmental biology

    2019  Band 7, Seite(n) 106

    Abstract: During cell spreading, cells undergo many changes to their architecture and their mechanical properties. Vimentin, as an integral part of the cell architecture, and its mechanical stability must adapt to the new state of the cell. This study focuses on ... ...

    Abstract During cell spreading, cells undergo many changes to their architecture and their mechanical properties. Vimentin, as an integral part of the cell architecture, and its mechanical stability must adapt to the new state of the cell. This study focuses on the structures formed by vimentin during the first steps of cell adhesion. Very early, ball-like structures, or "knots," are seen and often vimentin filaments emerge in the shape of rings around the nucleus. Although intermediate filaments are not known to be associated to motor proteins to form contractile systems, these rings can nonetheless strongly deform the cell nucleus. In the first 6 to 12 h of adhesion, these vimentin knots and rings disappear, and the intermediate filament network returns to the state seen before detachment of the cells. As these vimentin structures are very transient in the early steps of cell spreading, they have rarely been described in the literature. However, they can also be seen during mitosis, which is an event that involves partial detachment and re-spreading of the cells. Interestingly, the turnover dynamics of vimentin are reduced in both the knots and rings, compared to vimentin in the lamellipodia. It remains to define how the force is transmitted from the ball-like structures to the rings, and to measure the impact of such strong nuclear deformation on gene expression during cell re-spreading and the rearrangement of the vimentin network.
    Sprache Englisch
    Erscheinungsdatum 2019-06-17
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2019.00106
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