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  1. Article ; Online: Probing the structural states of human immunodeficiency virus type 1 pr55gag by using monoclonal antibodies.

    Leblanc, Jason J / Perez, Omar / Hope, Thomas J

    Journal of virology

    2007  Volume 82, Issue 5, Page(s) 2570–2574

    Abstract: Gag-FP (fluorescent protein) fusion constructs are commonly used to study human immunodeficiency virus type 1 assembly, yielding diffuse signals throughout the cytoplasm along with punctate signals routinely described as virus-like particles (VLPs) ... ...

    Abstract Gag-FP (fluorescent protein) fusion constructs are commonly used to study human immunodeficiency virus type 1 assembly, yielding diffuse signals throughout the cytoplasm along with punctate signals routinely described as virus-like particles (VLPs) representing assembled but unprocessed Gag. However, these particles cannot be accurately described as VLPs, since fluorescence microscopy cannot provide structural resolution. We demonstrate here that the inability of a monoclonal p24 antibody to bind its cognate epitope when unprocessed Gag is assembled distinguishes VLPs from unassembled, monomeric Gag. Furthermore, we show that assembled and unassembled Gag punctate signals travel along microtubules. These monoclonal antibody studies provide a new tool for examining retroviral assembly.
    MeSH term(s) Antibodies, Monoclonal/immunology ; Epitopes/immunology ; HIV-1/immunology ; HeLa Cells ; Humans ; Protein Conformation ; Protein Precursors/chemistry ; Protein Precursors/immunology
    Chemical Substances Antibodies, Monoclonal ; Epitopes ; Protein Precursors ; p55 gag precursor protein, Human immunodeficiency virus 1
    Language English
    Publishing date 2007-12-19
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/JVI.01717-07
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 609: Show issues Location:
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  2. Article ; Online: Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform.

    Leblanc, Jason J / Pettipas, Janice / Davidson, Ross J / Tipples, Graham A / Hiebert, Joanne / Hatchette, Todd F

    Journal of clinical microbiology

    2008  Volume 46, Issue 12, Page(s) 4049–4051

    Abstract: A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and ... ...

    Abstract A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
    MeSH term(s) Humans ; Mumps/diagnosis ; Mumps/virology ; Mumps virus/genetics ; Mumps virus/isolation & purification ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Ribonuclease P/genetics ; Sensitivity and Specificity ; Viral Fusion Proteins/genetics
    Chemical Substances RNA, Viral ; Viral Fusion Proteins ; Ribonuclease P (EC 3.1.26.5)
    Keywords covid19
    Language English
    Publishing date 2008-12
    Publishing country United States
    Document type Comparative Study ; Evaluation Studies ; Journal Article
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/JCM.01446-08
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1188: Show issues Location:
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  3. Article: Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.

    Leblanc, Jason J / Pettipas, Janice / Campbell, Sarah J / Davidson, Ross J / Hatchette, Todd F

    Journal of virological methods

    2008  Volume 151, Issue 1, Page(s) 158–160

    Abstract: Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent ... ...

    Abstract Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added.
    MeSH term(s) DNA Probes ; DNA, Viral/analysis ; DNA, Viral/metabolism ; Enzyme Stability ; Herpes Simplex/virology ; Herpesvirus 1, Human/genetics ; Herpesvirus 1, Human/isolation & purification ; Herpesvirus 2, Human/genetics ; Herpesvirus 2, Human/isolation & purification ; Hot Temperature ; Humans ; Nucleic Acid Hybridization/methods ; Polymerase Chain Reaction/methods ; Transition Temperature ; Uracil-DNA Glycosidase/metabolism
    Chemical Substances DNA Probes ; DNA, Viral ; Uracil-DNA Glycosidase (EC 3.2.2.-)
    Language English
    Publishing date 2008-07
    Publishing country Netherlands
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 8013-5
    ISSN 1879-0984 ; 0166-0934
    ISSN (online) 1879-0984
    ISSN 0166-0934
    DOI 10.1016/j.jviromet.2008.03.024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1565: Show issues Location:
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  4. Article: Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform

    Leblanc, Jason J / Pettipas, Janice / Davidson, Ross J / Tipples, Graham A / Hiebert, Joanne / Hatchette, Todd F

    Journal of clinical microbiology JCM. 2008 Dec., v. 46, no. 12

    2008  

    Abstract: A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and ... ...

    Abstract A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
    Language English
    Dates of publication 2008-12
    Size p. 4049-4051.
    Publishing place American Society for Microbiology
    Document type Article
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Association of antigen-stimulated release of tumor necrosis factor-alpha in whole blood with response to chemotherapy in patients with pulmonary multidrug-resistant tuberculosis.

    Eum, Seok-Yong / Lee, Ye-Jin / Min, Jin-Hong / Kwak, Hyun-Kyung / Hong, Min-Sun / Kong, Ji-Hye / Hwang, Soo-Hee / Park, Seung-Kyu / Leblanc, Jason J / Via, Laura E / Barry, Clifton E / Cho, Sang-Nae

    Respiration; international review of thoracic diseases

    2010  Volume 80, Issue 4, Page(s) 275–284

    Abstract: Background: We have previously reported that TNF-α levels correlate to total mycobacterial burden in tuberculosis (TB) patients.: Objective: To characterize the dynamics of cytokine responses in TB patients during chemotherapy to identify potential ... ...

    Abstract Background: We have previously reported that TNF-α levels correlate to total mycobacterial burden in tuberculosis (TB) patients.
    Objective: To characterize the dynamics of cytokine responses in TB patients during chemotherapy to identify potential surrogate markers for effective treatment.
    Methods: Following induction by culture filtrate proteins in whole blood, production patterns of TNF-α, IL-10, IFN-γ and IL-12 were measured in 23 non-multidrug-resistant (MDR)-TB and 16 MDR-TB patients and in 31 healthy controls. Rates of mycobacterial clearance from the sputum were then measured and compared.
    Results: Prior to the initiation of chemotherapy, TNF-α and IL-10 levels were significantly higher in TB patients than in healthy controls while IFN-γ and IL-12 levels were similar. During chemotherapy, the levels of all 4 cytokines increased. We evaluated these responses separately in patients that did and did not clear their sputum culture at 2 and 6 months. At 2 months, decreases in both IFN-γ and IL-12 correlated strongly with a successful early response, while after 6 months of therapy, when half (7/14) of MDR-TB patients were still sputum culture positive, downregulation of TNF-α was uniquely correlated with sputum conversion between the groups.
    Conclusion: Our findings suggest the possibility that the regulation of TNF-α production in whole blood may be a more specific indicator of sputum conversion at 6 months than IFN-γ, IL-12 or IL-10 in MDR-TB patients.
    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Antitubercular Agents/therapeutic use ; Biomarkers/blood ; Case-Control Studies ; Female ; Humans ; Interferon-gamma/blood ; Interleukin-10/blood ; Interleukin-12/blood ; Male ; Middle Aged ; Mycobacterium tuberculosis/isolation & purification ; Sputum/microbiology ; Tuberculosis, Multidrug-Resistant/blood ; Tuberculosis, Multidrug-Resistant/drug therapy ; Tumor Necrosis Factor-alpha/blood ; Young Adult
    Chemical Substances Antitubercular Agents ; Biomarkers ; IL10 protein, human ; Tumor Necrosis Factor-alpha ; Interleukin-10 (130068-27-8) ; Interleukin-12 (187348-17-0) ; Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2010-02-10
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 206674-9
    ISSN 1423-0356 ; 0025-7931
    ISSN (online) 1423-0356
    ISSN 0025-7931
    DOI 10.1159/000283687
    Database MEDical Literature Analysis and Retrieval System OnLINE

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