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  1. Article ; Online: The study of mechanisms of biological activity of copper oxide nanoparticle CuO in the test for seedling roots of Triticum vulgare.

    Korotkova, Anastasia Mickhailovna / Lebedev, Svyatoslav Valeryevich / Gavrish, Irina Aleksandrovna

    Environmental science and pollution research international

    2017  Volume 24, Issue 11, Page(s) 10220–10233

    Abstract: The rapid development of nanotechnology raises questions assessment of their impact on living objects. In the present study, we evaluated the impact of nanoparticle (NP) CuO at concentrations ranging from 0.8 to 63.5 g/l in the test on wheat seedlings ... ...

    Abstract The rapid development of nanotechnology raises questions assessment of their impact on living objects. In the present study, we evaluated the impact of nanoparticle (NP) CuO at concentrations ranging from 0.8 to 63.5 g/l in the test on wheat seedlings Triticum vulgare during 1-72 h. In the viability test (WST-test), cells were isolated from the roots of seedlings T. vulgare, 12 h not observed increase reductase activity after 24 h decreased rate of not more than 19% compared with the control. The number of dead cells in seedlings of T. vulgaris after exposure with CuO nanoparticles to the test with Evans blue increased by 5-15% compared to control. We observed that a significant increase in copper revenues leaves 4.5-8.9 times more in relation to the control and the roots-in 5-9.7 times. During the determined amount of active oxygen species, a significant proportional increase in the total pool of reactive oxygen species (ROS) in roots increased to 27.6% after exposure to NP CuO compared with the control. It is shown that in the introduction in medium, the NP CuO in the doses ranging from 3.2 to 63.5 g/l leads to DNA fragmentation and increases the fragments less than 3000 bp on 51.4-62.8%. The totality of our results influences nanoforms of copper oxide on the amount of ROS, and the viability of the genomic component of the cells shows different mechanisms of damage in the activation of a metabolic reaction, to determine the concentration of nano-CuO.
    MeSH term(s) Copper/toxicity ; Metal Nanoparticles ; Oxides ; Seedlings ; Triticum
    Chemical Substances Oxides ; Copper (789U1901C5)
    Language English
    Publishing date 2017-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-017-8549-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Assessment of the toxicity of silicon nanooxide in relation to various components of the agroecosystem under the conditions of the model experiment

    Lebedev, Svyatoslav Valeryevich / Galaktionova, Lyudmila Vyacheslavovna / Gavrish, Irina Aleksandrovna / Korotkova, Anastasia Mickhailovna / Sizova, Elena Anatolyevna

    Environmental geochemistry and health. 2019 Apr., v. 41, no. 2

    2019  

    Abstract: Investigation of SiO2 nanoparticles (NPs) effect on Eisenia fetida showed no toxic effect of the metal at a concentration of 250, 500 and 1000 mg per kg of soil, but conversely, a biomass increase from 23.5 to 29.5% (at the protein level decrease from 60 ...

    Abstract Investigation of SiO2 nanoparticles (NPs) effect on Eisenia fetida showed no toxic effect of the metal at a concentration of 250, 500 and 1000 mg per kg of soil, but conversely, a biomass increase from 23.5 to 29.5% (at the protein level decrease from 60 to 80%). The reaction of the earthworm organism fermentative system was expressed in the decrease in the level of superoxide dismutase (SOD) on the 14th day and in the increase in its activity to 27% on the 28th day. The catalase level (CAT) showed low activity at average element concentrations and increase by 39.4% at a dose of 1000 mg/kg. Depression of malonic dialdehyde (MDA) was established at average concentrations of 11.2% and level increase up to 9.1% at a dose of 1000 mg/kg with the prolongation of the effect up to 87.5% after 28-day exposure. The change in the microbiocenosis of the earthworm intestine was manifested by a decrease in the number of ammonifiers (by 42.01–78.9%), as well as in the number of amylolytic microorganisms (by 31.7–65.8%). When the dose of SiO2 NPs increased from 100 to 1000 mg/kg, the number of Azotobacter increased (by 8.2–22.2%), while the number of cellulose-destroying microorganisms decreased to 71.4% at a maximum dose of 1000 mg/kg. The effect of SiO2 NPs on Triticum aestivum L. was noted in the form of a slight suppression of seed germination (no more than 25%), an increase in the length of roots and aerial organs which generally resulted in an increase in plant biomass. Assessing the soil microorganisms’ complex during introduction of metal into the germination medium of Triticum aestivum L., there was noted a decrease in the ammonifiers number (by 4.7–67.6%) with a maximum value at a dose of 1000 mg/kg. The number of microorganisms using mineral nitrogen decreased by 29.5–69.5% with a simultaneous increase in the number at a dose of 50 mg/kg (+ 20%). Depending on NP dose, there was an inhibition of the microscopic fungi development by 18.1–72.7% and an increase in the number of cellulose-destroying microorganisms. For all variants of the experiment, the activity of soil enzymes of the hydrolase and oxidoreductase classes was decreased.
    Keywords agroecosystems ; Azotobacter ; catalase ; earthworms ; Eisenia fetida ; fungi ; germination ; hydrolases ; intestines ; models ; nanoparticles ; nitrogen ; phytomass ; protein content ; roots ; silica ; silicon ; soil ; soil enzymes ; soil microorganisms ; superoxide dismutase ; toxicity ; Triticum aestivum
    Language English
    Dates of publication 2019-04
    Size p. 769-782.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 52039-1
    ISSN 1573-2983 ; 0142-7245 ; 0269-4042
    ISSN (online) 1573-2983
    ISSN 0142-7245 ; 0269-4042
    DOI 10.1007/s10653-018-0171-3
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Assessment of the toxicity of silicon nanooxide in relation to various components of the agroecosystem under the conditions of the model experiment.

    Lebedev, Svyatoslav Valeryevich / Gavrish, Irina Aleksandrovna / Galaktionova, Lyudmila Vyacheslavovna / Korotkova, Anastasia Mickhailovna / Sizova, Elena Anatolyevna

    Environmental geochemistry and health

    2018  Volume 41, Issue 2, Page(s) 769–782

    Abstract: Investigation of ... ...

    Abstract Investigation of SiO
    MeSH term(s) Animals ; Biomass ; Catalase/metabolism ; Nanoparticles ; Oligochaeta/drug effects ; Silicon Dioxide/toxicity ; Soil/chemistry ; Soil Pollutants/analysis ; Superoxide Dismutase/metabolism ; Triticum/metabolism
    Chemical Substances Soil ; Soil Pollutants ; Silicon Dioxide (7631-86-9) ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1)
    Language English
    Publishing date 2018-08-18
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 52039-1
    ISSN 1573-2983 ; 0142-7245 ; 0269-4042
    ISSN (online) 1573-2983
    ISSN 0142-7245 ; 0269-4042
    DOI 10.1007/s10653-018-0171-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

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  4. Article ; Online: Effect of probiotics on the basis of Bacillus subtilis and Bifidobacterium longum on the biochemical parameters of the animal organism.

    Kvan, Olga Vilorievna / Gavrish, Irina Aleksandrovna / Lebedev, Svyatoslav Valeryevich / Korotkova, Anastasia Mickhailovna / Miroshnikova, Elena Petrovna / Serdaeva, Victoria Alekseevna / Bykov, Artem Vladimirovich / Davydova, Natalia Olegovna

    Environmental science and pollution research international

    2017  Volume 25, Issue 3, Page(s) 2175–2183

    Abstract: For the purpose of safe modulation of the intestinal microflora, probiotics have been increasingly used in recent years. In the present work, the effect of the probiotic sporobacterin (Bacillus subtilis 534) (I group) and soybean-bifidum (Bifidobacterium ...

    Abstract For the purpose of safe modulation of the intestinal microflora, probiotics have been increasingly used in recent years. In the present work, the effect of the probiotic sporobacterin (Bacillus subtilis 534) (I group) and soybean-bifidum (Bifidobacterium longum) (II group) on male rats of the Wistar line was evaluated. In assessing nonspecific immunity in vitro, there was an increase in the level of baseline level in the first and second groups (by 8.3 and 12.2% more control). The influence of probiotic preparations on the intestinal normoflora was assessed using PCR. Bifidumbacterin increased the normal microflora, in particular, Escherichia coli 1.55 times, Lactobacillus 1.26 times, Enterococcus 1.3 times as much control; the level of conditionally pathogenic microflora, in particular, Proteus spp. decreased by 1.3 times in comparison with the control. Sporobacterin also contributed to an increase in the amount of E. coli (1.55 times) and Lactobacillus (0.9 times). When a culture of Bifidobacterium longum was introduced, a selective reduction in the loss of chemical elements was observed against the background of the diet used. At the end of the experiment, the content of calcium in the body tissues of animals of group II exceeded this indicator in group I by 3.9%, phosphorus by 17.6%, copper by 28.5%, and zinc by 15.2%. The totality of the results obtained by us indicates that inclusion of Bifidumbacterium longum in the diet of animals makes the use of this preparation in the correction of mineral imbalance and improves the microflora of the intestines of animals by reducing the number of representatives of opportunistic microflora against the background of an increase in the number of basic representatives of normal microbiocenosis. Also, the use of probiotic drugs as additives leads to a slight increase in the level of nonspecific immunity, which increases the natural resistance of the organism.
    MeSH term(s) Animals ; Bacillus subtilis/physiology ; Bifidobacterium longum/physiology ; Diet ; Escherichia coli/growth & development ; Gastrointestinal Microbiome ; Lactobacillus/growth & development ; Male ; Minerals ; Probiotics/pharmacology ; Proteus/growth & development ; Rats ; Rats, Wistar
    Chemical Substances Minerals
    Language English
    Publishing date 2017-11-07
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-017-0534-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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