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Article ; Online: DIO1 Gene Polymorphism Is Associated with Thyroid Profiles and Reproductive Performance in Dairy Cows

Kostyunina, Olga V. / Mityashova, Olga S. / Bardukov, Nikolay V. / Aleynikova, Olga V. / Lebedeva, Irina Y.

Agriculture. 2023 Feb. 08, v. 13, no. 2

2023

Abstract: Thyroid hormones mediate the interaction between the metabolic and reproductive systems, while their metabolism is controlled by different deiodinases. The present study aimed to search for associations of cow genotypes with SNPs in the deiodinase type 1 ...

Abstract	Thyroid hormones mediate the interaction between the metabolic and reproductive systems, while their metabolism is controlled by different deiodinases. The present study aimed to search for associations of cow genotypes with SNPs in the deiodinase type 1 gene (DIO1) with thyroid profiles and reproductive traits. The blood was sampled from Russian black-and-white cows 2–6 weeks before calving and 1–13 weeks after calving to measure the hormonal levels by ELISA. RT-PCR analysis was performed for known mutations in the bovine DIO1 gene, and a polymorphism at position 13,149 was found. In animals with the CG genotype, the blood concentration of reverse triiodothyronine 6 weeks prepartum was higher and decreased much earlier than in animals with the CC genotype. Furthermore, 1 week after calving, the total triiodothyronine to reverse triiodothyronine ratio in cows with the CG genotype was higher than in cows with the CC genotype. A higher proportion of animals with better values of fertility traits was revealed in the CC group compared to the CG group. Thus, cows with the CC genotype of the DIO1 gene more often have a high reproductive ability, which may be associated with the rT3 profile features during the prepartum and early postpartum periods.
Keywords	agriculture ; blood ; cows ; genes ; genetic polymorphism ; genotype ; metabolism ; reproductive performance ; reverse transcriptase polymerase chain reaction ; triiodothyronine
Language	English
Dates of publication	2023-0208
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture13020398
Database	NAL-Catalogue (AGRICOLA)

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Article: Delaying Effects of Prolactin and Growth Hormone on Aging Processes in Bovine Oocytes Matured In Vitro.

Singina, Galina N / Shedova, Ekaterina N / Lopukhov, Alexander V / Mityashova, Olga S / Lebedeva, Irina Y

Pharmaceuticals (Basel, Switzerland)

2021 Volume 14, Issue 7

Abstract: Aging processes accelerate dramatically in oocytes that have reached the metaphase-II (M-II) stage. The present work aimed to study the patterns and intracellular pathways of actions of prolactin (PRL) and growth hormone (GH) on age-associated changes in ...

Abstract	Aging processes accelerate dramatically in oocytes that have reached the metaphase-II (M-II) stage. The present work aimed to study the patterns and intracellular pathways of actions of prolactin (PRL) and growth hormone (GH) on age-associated changes in bovine M-II oocytes aging in vitro. To this end, we analyzed spontaneous parthenogenetic activation (cytogenetic assay), apoptosis (TUNEL assay), and the developmental capacity (IVF/IVC) of in vitro-matured oocytes after prolonged culturing. Both PRL and GH reduced the activation rate of aging cumulus-enclosed oocytes (CEOs) and denuded oocytes (DOs), and their respective hormone receptors were revealed in the ova. The inhibitor of Src-family tyrosine kinases PP2 eliminated the effects of PRL and GH on meiotic arrest in DOs, whereas the MEK inhibitor U0126 only abolished the PRL effect. Furthermore, PRL was able to maintain the apoptosis resistance and developmental competence of aging CEOs. The protein kinase C inhibitor calphostin C suppressed both the actions of PRL. Thus, PRL and GH can directly support meiotic arrest in aging M-II oocytes by activating MAP kinases and/or Src-family kinases. The effect of PRL in maintaining the developmental capacity of aging oocytes is cumulus-dependent and related to the pro-survival action of the protein kinase C-mediated signal pathway.
Language	English
Publishing date	2021-07-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph14070684
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Article: Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways.

Lebedeva, Irina Y / Singina, Galina N / Lopukhov, Alexander V / Shedova, Ekaterina N / Zinovieva, Natalia A

Frontiers in genetics

2015 Volume 6, Page(s) 274

Abstract: General senescence of the adult organism is closely connected with reproductive one. Meanwhile, the age-related reduction in the female fertility is primarily associated with a decline in the gamete quality. Molecular and cellular changes in oocytes of ... ...

Abstract	General senescence of the adult organism is closely connected with reproductive one. Meanwhile, the age-related reduction in the female fertility is primarily associated with a decline in the gamete quality. Molecular and cellular changes in oocytes of old mammalian females are very similar to those occurring during aging of matured ova of their young counterparts, suggesting similarities in underlying mechanisms. The aim of the present work was to study actions of two related pituitary hormones, prolactin (PRL) and growth hormone (GH), on age-associated modifications of metaphase-II (M-II) chromosomes in bovine oocytes using a model of the prolonged culture. We analyzed: (1) effects of PRL and GH on abnormal changes in the chromosome morphology in aging matured oocytes and the role of cumulus cells in these effects and (2) signaling pathways involved in the hormone actions. During the prolonged culture of oocytes, a gradual rise in the frequency of destructive modifications of M-II chromosomes was revealed. In the case of cumulus-enclosed oocytes (CEOs), PRL and GH exerted dose-dependent biphasic effects on the frequency of these modifications. Both PRL (50 ng/ml) and GH (10 ng/ml) decelerated the abnormal chromosome changes in CEOs, but did not affect the chromosome configuration in denuded oocytes. Concurrently, the presence of PRL and GH receptors in cumulus cells surrounding matured oocytes was demonstrated. Attenuating effects of both hormones on the chromosome modifications in aging CEOs were abolished by PP2 (an inhibitor of Src-family tyrosine kinases), triciribine (an inhibitor of Akt kinase), and calphostin C (a protein kinase C inhibitor). Our findings indicate that PRL and GH can exert the similar decelerating action on age-associated alterations in the M-II chromosome morphology in bovine ova, which is mediated by cumulus cells and may be related to activation of Src-family tyrosine kinases as well as Akt- and protein kinase C-dependent signal pathways.
Language	English
Publishing date	2015-08-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2015.00274
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Article ; Online: Age-dependent role of steroids in the regulation of growth of the hen follicular wall

Lebedev Vladimir A / Lebedeva Irina Y / Grossmann Roland / Parvizi Nahid

Reproductive Biology and Endocrinology, Vol 8, Iss 1, p

2010 Volume 15

Abstract: Abstract Background The ovaries are the primary targets of senescence effects in mammalian and avian species. In the present study, relationships between reproductive aging, sex steroids and the growth pattern of the pre-ovulatory follicle wall were ... ...

Abstract	Abstract Background The ovaries are the primary targets of senescence effects in mammalian and avian species. In the present study, relationships between reproductive aging, sex steroids and the growth pattern of the pre-ovulatory follicle wall were investigated using young hens with long clutch (YLC), old hens with long clutch (OLC), old hens with short clutch (OSC), and old hens with interrupted long clutch (OILC). Methods Experiment 1: Hens were sacrificed 1.5 and 14.5 h after ovulation. Experiment 2: YLC and OILC hens were sacrificed 3.5 h after treatments with LH and/or aminoglutethimide (AG), an inhibitor of steroid synthesis. Volumes of pre-ovulatory follicles (F1-F5) and plasma concentrations of ovarian steroids were determined. Experiment 3: Granulosa and theca cells from F3 follicles of OSC and/or YLC hens were exposed in vitro to estradiol-17beta (E 2 ), testosterone (T) and LH and the proliferative activity of the cells was examined using CellTiter 96 Aqueous One Solution Assay. Results In YLC and OLC groups, the total volume of F1-F5 follicles rose between 1.5 and 14.5 h after ovulation (P < 0.01), negatively correlating with the plasma level of E 2 (P < 0.01). There was no growth of pre-ovulatory follicles in the middle of the ovulatory cycle in the OSC group, with a positive correlation being present between E 2 and the follicular volume (P < 0.05). In young hens, AG caused a rise in the total follicular volume. This rise was associated with a fall in E 2 (r = -0.54, P < 0.05). E 2 enhanced proliferation of granulosa cells from YLC and OSC groups. The proliferative activity of granulosa and theca cells of YLC hens depended on the interaction between T and LH (P < 0.01). Conclusions These data indicate for the first time that the growth pattern of pre-ovulatory follicles during the ovulatory cycle changes in the course of reproductive aging. E 2 seems to play a dual role in this adjustment; it stimulates the growth of the follicular wall in reproductive aged hens, whereas it may inhibit this process in young birds. T and LH are apparently involved in the growth regulation during the pre-ovulatory surge in young hens.
Keywords	Physiology ; QP1-981 ; Science ; Q ; DOAJ:Physiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Subject code	630
Language	English
Publishing date	2010-02-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Prolactin affects bovine oocytes through direct and cumulus-mediated pathways.

Lebedeva, Irina Y / Singina, Galina N / Volkova, Natalia A / Vejlsted, Morten / Zinovieva, Natalia A / Schmidt, Mette

Theriogenology

2014 Volume 82, Issue 8, Page(s) 1154–1164

Abstract: The available evidence points to participation of PRL in regulation of mammalian oocyte maturation. The aim of the present study was to characterize pathways of PRL action on bovine oocytes. We analyzed (1) the presence of the PRL receptor and its mRNA ... ...

Abstract	The available evidence points to participation of PRL in regulation of mammalian oocyte maturation. The aim of the present study was to characterize pathways of PRL action on bovine oocytes. We analyzed (1) the presence of the PRL receptor and its mRNA isoforms in oocytes and cumulus cells; (2) the effect of PRL on meiosis resumption and the role of cumulus cells, the NO/NO synthase system, protein kinase C, and tyrosine kinases in this effect; and (3) PRL effects in the presence of gonadotropins on the developmental capacity of cumulus-free and cumulus-enclosed oocytes. The transcript and protein expression of the PRL receptor in the cells were detected by reverse transcription polymerase chain reaction and immunocytochemistry, respectively. The nuclear status of oocytes was assessed after culture of cumulus-oocyte complexes (COCs) and denuded oocytes (DOs) with or without PRL (5-500 ng/mL) for 7, 14, or 24 hours. Besides, DOs were incubated for 7 hours in the absence or the presence of PRL (50 ng/mL) and/or L-NAME (an inhibitor of NO synthase), genistein (an inhibitor of tyrosine kinases), or calpostin C (a protein kinase C inhibitor). After IVM in 2 different systems containing PRL (50 ng/mL) and/or gonadotropic hormones, a part of oocytes underwent IVF and IVC and the embryo development was tracked until the blastocyst stage. Messenger RNA of long and short isoforms of the PRL receptor was revealed in both oocytes and cumulus cells. Immunocytochemistry confirmed the presence of the PRL receptor in oocytes and the cumulus investment. In the absence of gonadotropins (system 1), PRL retarded meiosis resumption in DOs but not in cumulus-enclosed oocytes, with this effect being short term, dose dependent, suppressed by L-NAME and genistein, and unaffected by calpostin. In systems containing gonadotropins, PRL did not affect nuclear maturation and the cleavage rate of cumulus-free and cumulus-enclosed oocytes. However, in the case of COCs, it raised the blastocyst yield both in system 2 (from 20.5%-40.9%, P < 0.01) and in system 3 (from 21.7%-33.9%, P < 0.05). The findings show for the first time the functioning of the direct pathway of PRL signaling into bovine oocytes, as confirmed by the expression of receptors of PRL and its direct meiosis-retarding effect involving activation of tyrosine kinases and NO synthase. Furthermore, this is the first demonstration that the beneficial effect of PRL on the oocyte developmental capacity is achieved via cumulus cells containing PRL receptors.
MeSH term(s)	Animals ; Cattle ; Cells, Cultured ; Cumulus Cells/chemistry ; Cumulus Cells/physiology ; Embryonic Development ; Female ; Fertilization in Vitro/veterinary ; In Vitro Oocyte Maturation Techniques/veterinary ; Meiosis/drug effects ; Nitric Oxide/physiology ; Nitric Oxide Synthase/metabolism ; Oocytes/chemistry ; Oocytes/drug effects ; Prolactin/pharmacology ; Protein Kinase C/metabolism ; Protein-Tyrosine Kinases/metabolism ; RNA, Messenger/analysis ; Receptors, Prolactin/analysis ; Receptors, Prolactin/genetics ; Signal Transduction
Chemical Substances	RNA, Messenger ; Receptors, Prolactin ; Nitric Oxide (31C4KY9ESH) ; Prolactin (9002-62-4) ; Nitric Oxide Synthase (EC 1.14.13.39) ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; Protein Kinase C (EC 2.7.11.13)
Language	English
Publishing date	2014-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2014.08.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Prolactin affects bovine oocytes through direct and cumulus-mediated pathways

Lebedeva, Irina Y / Galina N. Singina / Mette Schmidt / Morten Vejlsted / Natalia A. Volkova / Natalia A. Zinovieva

Theriogenology. 2014 Nov., v. 82, no. 8

2014

Abstract	The available evidence points to participation of PRL in regulation of mammalian oocyte maturation. The aim of the present study was to characterize pathways of PRL action on bovine oocytes. We analyzed (1) the presence of the PRL receptor and its mRNA isoforms in oocytes and cumulus cells; (2) the effect of PRL on meiosis resumption and the role of cumulus cells, the NO/NO synthase system, protein kinase C, and tyrosine kinases in this effect; and (3) PRL effects in the presence of gonadotropins on the developmental capacity of cumulus-free and cumulus-enclosed oocytes. The transcript and protein expression of the PRL receptor in the cells were detected by reverse transcription polymerase chain reaction and immunocytochemistry, respectively. The nuclear status of oocytes was assessed after culture of cumulus–oocyte complexes (COCs) and denuded oocytes (DOs) with or without PRL (5–500 ng/mL) for 7, 14, or 24 hours. Besides, DOs were incubated for 7 hours in the absence or the presence of PRL (50 ng/mL) and/or L-NAME (an inhibitor of NO synthase), genistein (an inhibitor of tyrosine kinases), or calpostin C (a protein kinase C inhibitor). After IVM in 2 different systems containing PRL (50 ng/mL) and/or gonadotropic hormones, a part of oocytes underwent IVF and IVC and the embryo development was tracked until the blastocyst stage. Messenger RNA of long and short isoforms of the PRL receptor was revealed in both oocytes and cumulus cells. Immunocytochemistry confirmed the presence of the PRL receptor in oocytes and the cumulus investment. In the absence of gonadotropins (system 1), PRL retarded meiosis resumption in DOs but not in cumulus-enclosed oocytes, with this effect being short term, dose dependent, suppressed by L-NAME and genistein, and unaffected by calpostin. In systems containing gonadotropins, PRL did not affect nuclear maturation and the cleavage rate of cumulus-free and cumulus-enclosed oocytes. However, in the case of COCs, it raised the blastocyst yield both in system 2 (from 20.5%–40.9%, P < 0.01) and in system 3 (from 21.7%–33.9%, P < 0.05). The findings show for the first time the functioning of the direct pathway of PRL signaling into bovine oocytes, as confirmed by the expression of receptors of PRL and its direct meiosis-retarding effect involving activation of tyrosine kinases and NO synthase. Furthermore, this is the first demonstration that the beneficial effect of PRL on the oocyte developmental capacity is achieved via cumulus cells containing PRL receptors.
Keywords	blastocyst ; cattle ; dose response ; embryogenesis ; genistein ; immunocytochemistry ; in vitro fertilization ; meiosis ; messenger RNA ; nitric oxide ; nitric oxide synthase ; oocytes ; prolactin ; protein kinase C ; protein synthesis ; receptors ; reverse transcriptase polymerase chain reaction ; tyrosine
Language	English
Dates of publication	2014-11
Size	p. 1154-1164.e1.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2014.08.005
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Age-dependent role of steroids in the regulation of growth of the hen follicular wall.

Lebedeva, Irina Y / Lebedev, Vladimir A / Grossmann, Roland / Parvizi, Nahid

Reproductive biology and endocrinology : RB&E

2010 Volume 8, Page(s) 15

Abstract: Background: The ovaries are the primary targets of senescence effects in mammalian and avian species. In the present study, relationships between reproductive aging, sex steroids and the growth pattern of the pre-ovulatory follicle wall were ... ...

Abstract	Background: The ovaries are the primary targets of senescence effects in mammalian and avian species. In the present study, relationships between reproductive aging, sex steroids and the growth pattern of the pre-ovulatory follicle wall were investigated using young hens with long clutch (YLC), old hens with long clutch (OLC), old hens with short clutch (OSC), and old hens with interrupted long clutch (OILC). Methods: Experiment 1: Hens were sacrificed 1.5 and 14.5 h after ovulation. Experiment 2: YLC and OILC hens were sacrificed 3.5 h after treatments with LH and/or aminoglutethimide (AG), an inhibitor of steroid synthesis. Volumes of pre-ovulatory follicles (F1-F5) and plasma concentrations of ovarian steroids were determined. Experiment 3: Granulosa and theca cells from F3 follicles of OSC and/or YLC hens were exposed in vitro to estradiol-17beta (E2), testosterone (T) and LH and the proliferative activity of the cells was examined using CellTiter 96 Aqueous One Solution Assay. Results: In YLC and OLC groups, the total volume of F1-F5 follicles rose between 1.5 and 14.5 h after ovulation (P < 0.01), negatively correlating with the plasma level of E2 (P < 0.01). There was no growth of pre-ovulatory follicles in the middle of the ovulatory cycle in the OSC group, with a positive correlation being present between E2 and the follicular volume (P < 0.05). In young hens, AG caused a rise in the total follicular volume. This rise was associated with a fall in E2 (r = -0.54, P < 0.05). E2 enhanced proliferation of granulosa cells from YLC and OSC groups. The proliferative activity of granulosa and theca cells of YLC hens depended on the interaction between T and LH (P < 0.01). Conclusions: These data indicate for the first time that the growth pattern of pre-ovulatory follicles during the ovulatory cycle changes in the course of reproductive aging. E2 seems to play a dual role in this adjustment; it stimulates the growth of the follicular wall in reproductive aged hens, whereas it may inhibit this process in young birds. T and LH are apparently involved in the growth regulation during the pre-ovulatory surge in young hens.
MeSH term(s)	Age Factors ; Aging/blood ; Aging/metabolism ; Aging/physiology ; Aminoglutethimide/administration & dosage ; Aminoglutethimide/pharmacology ; Animals ; Aromatase Inhibitors/administration & dosage ; Aromatase Inhibitors/pharmacology ; Cell Membrane/drug effects ; Cell Membrane/physiology ; Cell Proliferation/drug effects ; Cells, Cultured ; Chickens ; Female ; Gonadal Steroid Hormones/blood ; Gonadal Steroid Hormones/metabolism ; Gonadal Steroid Hormones/pharmacology ; Granulosa Cells/drug effects ; Granulosa Cells/metabolism ; Injections ; Luteinizing Hormone/administration & dosage ; Luteinizing Hormone/pharmacology ; Ovarian Follicle/cytology ; Ovarian Follicle/drug effects ; Ovarian Follicle/growth & development ; Ovarian Follicle/physiology ; Reproduction/physiology ; Theca Cells/drug effects ; Theca Cells/metabolism
Chemical Substances	Aromatase Inhibitors ; Gonadal Steroid Hormones ; Aminoglutethimide (0O54ZQ14I9) ; Luteinizing Hormone (9002-67-9)
Language	English
Publishing date	2010-02-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1477-7827
ISSN (online)	1477-7827
DOI	10.1186/1477-7827-8-15
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Age-dependent role of steroids in the regulation of growth of the hen follicular wall

Lebedeva, Irina Y. / Lebedev, Vladimir A. / Großmann, Roland / Parvizi, Nahid

2010

Abstract	Background: The ovaries are the primary targets of senescence effects in mammalian and avian species. In the present study, relationships between reproductive aging, sex steroids and the growth pattern of the pre-ovulatory follicle wall were investigated using young hens with long clutch (YLC), old hens with long clutch (OLC), old hens with short clutch (OSC), and old hens with interrupted long clutch (OILC). Methods: Experiment 1: Hens were sacrificed 1.5 and 14.5 h after ovulation. Experiment 2: YLC and OILC hens were sacrificed 3.5 h after treatments with LH and/or aminoglutethimide (AG), an inhibitor of steroid synthesis. Volumes of pre-ovulatory follicles (F1-F5) and plasma concentrations of ovarian steroids were determined. Experiment 3: Granulosa and theca cells from F3 follicles of OSC and/or YLC hens were exposed in vitro to estradiol-17beta (E-2), testosterone (T) and LH and the proliferative activity of the cells was examined using CellTiter 96 Aqueous One Solution Assay. Results: In YLC and OLC groups, the total volume of F1-F5 follicles rose between 1.5 and 14.5 h after ovulation (P < 0.01), negatively correlating with the plasma level of E-2 (P < 0.01). There was no growth of pre-ovulatory follicles in the middle of the ovulatory cycle in the OSC group, with a positive correlation being present between E-2 and the follicular volume (P < 0.05). In young hens, AG caused a rise in the total follicular volume. This rise was associated with a fall in E-2 (r = -0.54, P < 0.05). E-2 enhanced proliferation of granulosa cells from YLC and OSC groups. The proliferative activity of granulosa and theca cells of YLC hens depended on the interaction between T and LH (P < 0.01). Conclusions: These data indicate for the first time that the growth pattern of pre-ovulatory follicles during the ovulatory cycle changes in the course of reproductive aging. E-2 seems to play a dual role in this adjustment; it stimulates the growth of the follicular wall in reproductive aged hens, whereas it may inhibit this process in young birds. T and LH are apparently involved in the growth regulation during the pre-ovulatory surge in young hens
Keywords	ddc:630 ; EGG-PRODUCTION ; ESTROGEN-RECEPTOR ; FOWL GALLUS-DOMESTICUS ; GRANULOSA-CELLS ; LAYING HENS ; LUTEINIZING-HORMONE ; MESSENGER-RNA ; OVULATORY CYCLE ; PREOVULATORY OVARIAN-FOLLICLE ; THECAL CELLS
Subject code	630
Language	English
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Age-dependent role of steroids in the regulation of growth of the hen follicular wall

Lebedeva, Irina Y. / Lebedev, Vladimir A. / Großmann, Roland / Parvizi, Nahid

2010

Abstract	Background: The ovaries are the primary targets of senescence effects in mammalian and avian species. In the present study, relationships between reproductive aging, sex steroids and the growth pattern of the pre-ovulatory follicle wall were investigated using young hens with long clutch (YLC), old hens with long clutch (OLC), old hens with short clutch (OSC), and old hens with interrupted long clutch (OILC). Methods: Experiment 1: Hens were sacrificed 1.5 and 14.5 h after ovulation. Experiment 2: YLC and OILC hens were sacrificed 3.5 h after treatments with LH and/or aminoglutethimide (AG), an inhibitor of steroid synthesis. Volumes of pre-ovulatory follicles (F1-F5) and plasma concentrations of ovarian steroids were determined. Experiment 3: Granulosa and theca cells from F3 follicles of OSC and/or YLC hens were exposed in vitro to estradiol-17beta (E-2), testosterone (T) and LH and the proliferative activity of the cells was examined using CellTiter 96 Aqueous One Solution Assay. Results: In YLC and OLC groups, the total volume of F1-F5 follicles rose between 1.5 and 14.5 h after ovulation (P < 0.01), negatively correlating with the plasma level of E-2 (P < 0.01). There was no growth of pre-ovulatory follicles in the middle of the ovulatory cycle in the OSC group, with a positive correlation being present between E-2 and the follicular volume (P < 0.05). In young hens, AG caused a rise in the total follicular volume. This rise was associated with a fall in E-2 (r = -0.54, P < 0.05). E-2 enhanced proliferation of granulosa cells from YLC and OSC groups. The proliferative activity of granulosa and theca cells of YLC hens depended on the interaction between T and LH (P < 0.01). Conclusions: These data indicate for the first time that the growth pattern of pre-ovulatory follicles during the ovulatory cycle changes in the course of reproductive aging. E-2 seems to play a dual role in this adjustment; it stimulates the growth of the follicular wall in reproductive aged hens, whereas it may inhibit ...
Keywords	Text ; ddc:630 ; EGG-PRODUCTION ; ESTROGEN-RECEPTOR ; FOWL GALLUS-DOMESTICUS ; GRANULOSA-CELLS ; LAYING HENS ; LUTEINIZING-HORMONE ; MESSENGER-RNA ; OVULATORY CYCLE ; PREOVULATORY OVARIAN-FOLLICLE ; THECAL CELLS
Subject code	630
Language	English
Publishing country	de
Document type	Article ; Online
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Article: In vitro maturation and early developmental capacity of bovine oocytes cultured in pure follicular fluid and supplementation with follicular wall.

Coleman, Natalia V / Shagiakhmetova, Galina A / Lebedeva, Irina Y / Kuzmina, Tatiana I / Golubev, Alexander K

Theriogenology

2007 Volume 67, Issue 5, Page(s) 1053–1059

Abstract: Mammalian oocytes mature in follicular fluid (FF), surrounded by follicular cells. In the present study, in vitro maturation of bovine oocytes cultured in FF from dominant follicles 15-17mm in diameter (with various forms of heat pretreatment) and ... ...

Abstract	Mammalian oocytes mature in follicular fluid (FF), surrounded by follicular cells. In the present study, in vitro maturation of bovine oocytes cultured in FF from dominant follicles 15-17mm in diameter (with various forms of heat pretreatment) and supplementation with follicular wall from follicles 3-5mm in diameter (FW1) were examined. Heat pretreatment of FF was as follows: (1) no treatment (FF1); (2) 56 degrees C for 30min (FF2); and (3) 100 degrees C for 20s (FF3). After IVM in FF1, oocytes underwent IVF and IVC and embryo development was assessed (up to the morula stage). The rate of oocyte maturation was decreased in pure FF1 versus control (44.5% versus 62.8%, P<0.001). In the control medium, FW1 did not significantly affect nuclear maturation. By contrast, the addition of FW1 to FF1 increased the rate of matured oocytes approximately two-fold (85.9% versus 45.6%, P<0.001). Furthermore, the maturation rate in the FF+FW1 system declined (from 85.9 to 71.0%, P<0.001), whereas that in the FF system increased (from 45.6 to 71.6%, P<0.001) with increased temperature of the FF treatment. Supplementation of the control medium with FW1 increased the yield of morulae (42.6% versus 13.7%, P<0.001). However, the stimulatory effect of FW1 on the morula rate was much higher in pure FF1 (72.5% versus 31.7%, P<0.001). These findings indicated, for the first time, the stimulatory impact of FW1 on in vitro maturation and early developmental capacity of bovine oocytes cultured in pure FF from dominant follicles. We also inferred that bovine FF constituents affecting bovine oocyte maturation and the meiosis-promoting ability of the FW were heat-labile.
MeSH term(s)	Animals ; Cattle/physiology ; Cell Culture Techniques/methods ; Cell Culture Techniques/veterinary ; Culture Media/pharmacology ; Embryonic Development/physiology ; Female ; Fertilization in Vitro/veterinary ; Follicular Fluid/cytology ; Follicular Fluid/physiology ; Male ; Oocytes/cytology ; Oocytes/physiology ; Ovarian Follicle/cytology ; Ovarian Follicle/physiology
Chemical Substances	Culture Media
Language	English
Publishing date	2007-03-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2006.10.019
Database	MEDical Literature Analysis and Retrieval System OnLINE

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