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Article: Simulation en santé en formation initiale : un dispositif au bénéfice des apprenants.

Appriou-Ledesma, Laurence / Dorbon, Christelle

2023 Volume 72, Issue 288, Page(s) 27–29

Abstract: Three collaborative action-research projects have been carried out at the Institut de formation interhospitalier Théodore-Simon in Neuilly-sur-Marne (93) since the implementation of health simulation in nursing training. Their descriptions show the ... ...

Title translation	Health simulation in initial training: a device for the benefit of learners.
Abstract	Three collaborative action-research projects have been carried out at the Institut de formation interhospitalier Théodore-Simon in Neuilly-sur-Marne (93) since the implementation of health simulation in nursing training. Their descriptions show the interest and the advantages of this pedagogical method and of the different "action pedagogies" which result from it for the nursing learners.
MeSH term(s)	Humans ; Computer Simulation ; Education, Nursing/methods
Language	French
Publishing date	2023-01-23
Publishing country	France
Document type	English Abstract ; Journal Article
ZDB-ID	632538-5
ISSN	1293-8505 ; 0397-7900
ISSN	1293-8505 ; 0397-7900
DOI	10.1016/j.revinf.2023.01.027
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Article ; Online: Prediction of DNA-Binding Transcription Factors in Bacteria and Archaea Genomes.

Ledesma, Leonardo / Hernandez-Guerrero, Rafael / Perez-Rueda, Ernesto

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2516, Page(s) 103–112

Abstract: DNA-binding transcription factors (TFs) play a central role in the gene expression of all organisms, from viruses to humans, including bacteria and archaea. The role of these proteins is the fate of gene expression in the context of environmental ... ...

Abstract	DNA-binding transcription factors (TFs) play a central role in the gene expression of all organisms, from viruses to humans, including bacteria and archaea. The role of these proteins is the fate of gene expression in the context of environmental challenges. Because thousands of genomes have been sequenced to date, predictions of the encoded proteins are validated through the use of bioinformatics tools to obtain the necessary experimental, posterior knowledge. In this chapter, we describe three approaches to identify TFs in protein sequences. The first approach integrates the results of sequence comparisons and PFAM assignments, using as reference a manually curated collection of TFs. The second approach considers the prediction of DNA-binding structures, such as the classical helix-turn-helix (HTH); and the third approach considers a deep learning model. We suggest that all approaches must be considered together to increase the possibility of identifying new TFs in bacterial and archaeal genomes.
MeSH term(s)	Archaea/metabolism ; Bacteria/metabolism ; DNA/metabolism ; Genome, Archaeal/genetics ; Genome, Bacterial ; Humans ; Transcription Factors/metabolism
Chemical Substances	Transcription Factors ; DNA (9007-49-2)
Language	English
Publishing date	2022-08-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2413-5_7
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Article ; Online: Characterization of sperm motility and testosterone secretion in the

Muñoz de la Torre, Luz Patricia / Trujillo Hernández, Angélica / Eguibar, José Ramón / Cortés, Carmen / Morales-Ledesma, Leticia

Animal reproduction

2023 Volume 20, Issue 3, Page(s) e20220102

Abstract: Presently, demyelinating diseases have been reported to affect the reproductive life of patients who suffer from them, but the progression of the alterations is unknown, especially in men. To better understand these effects, it is necessary to perform ... ...

Abstract	Presently, demyelinating diseases have been reported to affect the reproductive life of patients who suffer from them, but the progression of the alterations is unknown, especially in men. To better understand these effects, it is necessary to perform studies in animal models, such as the male
Language	English
Publishing date	2023-10-30
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2227229-X
ISSN	1984-3143 ; 1984-3143
ISSN (online)	1984-3143
ISSN	1984-3143
DOI	10.1590/1984-3143-AR2022-0102
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Article: Administration of a VIP-antagonist in vivo modifies ovarian hormone secretion in a rat model with polycystic ovary syndrome

Morales-Ledesma, Leticia / Trujillo Hernández, Angélica / Ramírez, María Isabel / Rosas, Gabriela / Linares, Rosa

Life sciences. 2021 Jan. 15, v. 265

2021

Abstract: In the cyclic rat in estrus, the vasoactive intestinal peptide (VIP) has an impact on ovarian function, which depends on the endocrine status of the animal. In this work, we aimed to clarify the participation of VIP in the pathophysiological condition of ...

Abstract	In the cyclic rat in estrus, the vasoactive intestinal peptide (VIP) has an impact on ovarian function, which depends on the endocrine status of the animal. In this work, we aimed to clarify the participation of VIP in the pathophysiological condition of polycystic ovary syndrome (PCOS) using a model of PCOS induced by estradiol valerate (EV-PCOS) in rats.In the cyclic rat in estrus and in the EV-PCOS model, we analyzed the acute effects of blocking VIP receptors with the use of an antagonist (Ant-VIP) injected into the left or right ovarian bursa on the steroidogenic response and ovarian catecholamine levels.In the cyclic animal in estrus, the treatment with Ant-VIP in the left ovarian bursa resulted in a reduction in testosterone serum levels and in ovarian levels of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC), without changes in 4-hydroxy-3-methoxyphenyl (MHPG) and norepinephrine (NE). When the treatment was applied on the right side, only MHPG levels increased.In the EV-PCOS model, the treatment with Ant-VIP in the left ovarian bursa increased testosterone, estradiol, MHPG, and NE levels. When the treatment was performed on the right side, progesterone levels decreased and estradiol increased, without changes in ovarian catecholamines.The binding of VIP to its receptors differentially regulates steroidogenesis in the cyclic animal in estrus and in the EV-PCOS model. The blocking of VIP signaling produces changes in ovarian catecholamines.
Keywords	animal models ; animal ovaries ; antagonists ; blood serum ; dopamine ; estradiol ; estrus ; hormone secretion ; norepinephrine ; polycystic ovary syndrome ; progesterone ; rats ; steroidogenesis ; testosterone ; vasoactive intestinal peptide
Language	English
Dates of publication	2021-0115
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-light
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2020.118792
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Article ; Online: A multiphase texture-based model of active contours assisted by a convolutional neural network for automatic CT and MRI heart ventricle segmentation.

Carbajal-Degante, Erik / Avendaño, Steve / Ledesma, Leonardo / Olveres, Jimena / Vallejo, Enrique / Escalante-Ramirez, Boris

Computer methods and programs in biomedicine

2021 Volume 211, Page(s) 106373

Abstract: Background: Left and right ventricle automatic segmentation remains one of the more important tasks in computed aided diagnosis. Active contours have shown to be efficient for this task, however they often require user interaction to provide the initial ...

Abstract	Background: Left and right ventricle automatic segmentation remains one of the more important tasks in computed aided diagnosis. Active contours have shown to be efficient for this task, however they often require user interaction to provide the initial position, which drives the tool substantially dependent on a prior knowledge and a manual process. Methods: We propose to overcome this limitation with a Convolutional Neural Network (CNN) to reach the assumed target locations. This is followed by a novel multiphase active contour method based on texture that enhances whole heart patterns leading to an accurate identification of distinct regions, mainly left (LV) and right ventricle (RV) for the purposes of this work. Results: Experiments reveal that the initial location and estimated shape provided by the CNN are of great concern for the subsequent active contour stage. We assessed our method on two short data sets with Dice scores of 93% (LV-CT), 91% (LV-MRI), 0.86% (RV-CT) and 0.85% (RV-MRI). Conclusion: Our approach overcomes the performance of other techniques by means of a multiregion segmentation assisted by a CNN trained with a limited data set, a typical issue in medical imaging.
MeSH term(s)	Heart Ventricles/diagnostic imaging ; Image Processing, Computer-Assisted ; Magnetic Resonance Imaging ; Neural Networks, Computer ; Radiography ; Tomography, X-Ray Computed
Language	English
Publishing date	2021-08-31
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	632564-6
ISSN	1872-7565 ; 0169-2607
ISSN (online)	1872-7565
ISSN	0169-2607
DOI	10.1016/j.cmpb.2021.106373
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Article ; Online: Marika discussions in times of pandemic

Garzaniti, Ramiro / Díaz Ledesma, Lucas / Navarro Martínez, Ernesto

Question/Cuestión; Vol. 1 Núm. mayo (2020): Incidentes III. Parte I: Análisis/Orígenes; e292 ; 1669-6581

[re]precarization of life, right to health and biopolitics ; Discusiones marikas en tiempos de pandemia: [re]precarización de la vida, derecho a la salud y biopolítica

2020

Abstract: This essay aims to generate conditions for discussion around the scenes that we live in the obligatory social isolation context caused by Covid-19. Our main interest lies in enabling, from a gender/queer studies field (in dialogue with philosophy, social ...

Abstract	This essay aims to generate conditions for discussion around the scenes that we live in the obligatory social isolation context caused by Covid-19. Our main interest lies in enabling, from a gender/queer studies field (in dialogue with philosophy, social sciences and communication), a debate around the [re]precarization that the LGBT+ community is going through in the pandemic event. For this, we focus, in light of the debates that are being promoted by internationally recognized intellectuals, on axes such as (1) precarious lives and lives susceptible to harm in a capitalist, colonial-modern and hetero-cis system; (2) the tension, at the legal-political level, between redistribution and recognition, suggesting the right to health as the central dispute in the pandemic context; and (3) the bio[hetero]politicization of our queer lives. The ultimate goal of this material is to collaborate in the formulation of intelligibility modes that allow the emergence of new questions for the enablement, conformation and reconfiguration of strategies for collective intervention. Este texto tiene como objetivo generar las condiciones de discusión en torno a las escenas que vivimos en el marco del aislamiento social obligatorio a causa del Covid-19. Nuestro principal interés reside en habilitar, desde los estudios de género y disidentes (en diálogo con la filosofía, las ciencias sociales y la comunicación), un debate con respecto a la [re]precarización que el colectivo LGBT+ atraviesa en el contexto de la pandemia. Para ello, nos centramos, a la luz de los debates candentes que se promueven desde las voces intelectuales con reconocimiento internacional, en ejes como (1) las vidas precarias y las vidas susceptibles de dañabilidad en un sistema capitalista, colonial-moderno y cis-hetero; (2) la tensión, en el plano jurídico-político, entre redistribución y reconocimiento, señalando el derecho a la salud como eje central de disputa en el contexto pandémico; y (3) la bio[hetero]politización de nuestras existencias disidentes. El fin último de este material es colaborar en la formulación de modos de inteligibilidad que permitan la emergencia de nuevos interrogantes para la habilitación, conformación y reconfiguración de estrategias de intervención colectiva.
Keywords	covid19
Language	Spanish
Publishing date	2020-05-15
Publisher	Instituto de Investigaciones en Comunicación (IICOM)
Publishing country	ar
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Syrinx in Spinal Cord in Mummified Individual from West Thebes (Egypt).

Herrerin, Jesús / Prats, Albert / Ledesma, Lidia / Isidro, Albert

World neurosurgery

2018 Volume 118, Page(s) 230–234

Abstract: We analyzed a total of 36 partial or complete mummies containing neural structures from Sharuna and Qarara (Middle Egypt) and Dra Abu-el Naga, West Thebes (Upper Egypt). Individual TT16 13.3-B06-Ind07 corresponded to a partial mummy from T2 to T11. At ... ...

Abstract	We analyzed a total of 36 partial or complete mummies containing neural structures from Sharuna and Qarara (Middle Egypt) and Dra Abu-el Naga, West Thebes (Upper Egypt). Individual TT16 13.3-B06-Ind07 corresponded to a partial mummy from T2 to T11. At distal levels, it showed a structure compatible with the lower spinal cord (SC). Under magnification, the structure presented an absence of meningeal remains and a butterfly-like substructure resembling the anterior and posterior horns of the gray matter of the SC. Meanwhile, the central canal of the spinal cord (syrinx) was considerably enlarged. Field radiograph confirmed a structure compatible with the SC with a syrinx that shows a maximum diameter of 3.2 mm measured by digital caliper. Bearing in mind the normal shrinking mechanism at work in mummification, a pathologic condition such as syringomyelia during the individual's life is a distinct possibility. After a thorough review of the literature, this would be the earliest report of syringomyelia.
MeSH term(s)	Egypt/epidemiology ; History, Ancient ; Humans ; Mummies/diagnostic imaging ; Mummies/pathology ; Paleopathology/methods ; Spinal Cord/diagnostic imaging ; Spinal Cord/pathology ; Syringomyelia/diagnostic imaging ; Syringomyelia/epidemiology ; Syringomyelia/pathology
Language	English
Publishing date	2018-07-23
Publishing country	United States
Document type	Historical Article ; Journal Article ; Review
ZDB-ID	2534351-8
ISSN	1878-8769 ; 1878-8750
ISSN (online)	1878-8769
ISSN	1878-8750
DOI	10.1016/j.wneu.2018.07.110
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Article ; Online: Intrinsic innervation of the ovary and its variations in the rat senescence process.

Bravo-Benitez, Juan M / Cruz, Yolanda / Lucio, Rosa A / Venegas, Berenice / Díaz, Alfonso / Morales-Ledesma, Leticia / Domínguez, Roberto / Morán, Carolina

Journal of molecular histology

2022 Volume 53, Issue 2, Page(s) 347–356

Abstract: Ovarian functions decrease with perimenopause. The ovary has extrinsic innervation, but the neural influence on ovarian functions and dysfunction is not well-studied. The present study aimed to biochemically and morphometrically characterize the ... ...

Abstract	Ovarian functions decrease with perimenopause. The ovary has extrinsic innervation, but the neural influence on ovarian functions and dysfunction is not well-studied. The present study aimed to biochemically and morphometrically characterize the intrinsic neurons in ovaries from young adult, middle-aged, and senescent Long Evans CII-ZV rats (3, 12, and 15 months old, respectively). Ovaries were extracted from four rats of each age group (n = 12 total), cryopreserved, and processed for immunofluorescence studies with the primary NeuN/β-tubulin and NeuN/tyrosine hydroxylase (TH) antibodies. The soma area and number of intrinsic neurons in the ovarian stroma, surrounding follicles, corpus luteum, or cyst were evaluated. The intrinsic neurons were grouped in cluster-like shapes in ovarian structures. In senescent rats, the intrinsic neurons were mainly localized in the ovarian stroma and around the cysts. The number of neurons was lower in senescent rats than in young adult rats (p < 0.05), but the soma size was larger than in young adult rats. Immunoreactivity to TH indicated the presence of noradrenergic neurons in the ovary with the same characteristics as NeuN/β-tubulin, which indicates that they are part of the same neuronal group. Taken together, the findings indicate that the intrinsic neurons may be related to the loss of ovarian functions associated with aging.
MeSH term(s)	Aging ; Animals ; Female ; Ovary ; Rats ; Rats, Long-Evans ; Tubulin ; Tyrosine 3-Monooxygenase
Chemical Substances	Tubulin ; Tyrosine 3-Monooxygenase (EC 1.14.16.2)
Language	English
Publishing date	2022-02-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2144021-9
ISSN	1567-2387 ; 1567-2379
ISSN (online)	1567-2387
ISSN	1567-2379
DOI	10.1007/s10735-022-10069-7
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Article ; Online: Sex-specific hypothalamic expression of kisspeptin, gonadotropin releasing hormone, and kisspeptin receptor in progressive demyelination model.

Muñoz de la Torre, Luz Patricia / Trujillo Hernández, Angélica / Eguibar, Jose R / Cortés, Carmen / Morales-Ledesma, Leticia

Journal of chemical neuroanatomy

2022 Volume 123, Page(s) 102120

Abstract: Demyelinating diseases, such as multiple sclerosis, decrease the quality of life of patients and can affect reproduction. Assisted reproductive therapies are available, which although effective, aggravate motor symptoms. For this reason, it is important ... ...

Abstract	Demyelinating diseases, such as multiple sclerosis, decrease the quality of life of patients and can affect reproduction. Assisted reproductive therapies are available, which although effective, aggravate motor symptoms. For this reason, it is important to determine how the control of the hypothalamus-pituitary-gonadal axis is affected in order to develop better strategies for these patients. One way to determine this is using animal models such as the taiep rat, which shows progressive demyelination of the central nervous system, and was used in the present study to characterize the expression of gonadotrophin-releasing hormone (GnRH), Kisspeptin, and kisspeptin receptor (Kiss1R) and luteinizing hormone (LH) secretion. The expression of kisspeptin, GnRH, and Kiss1R was determined at the hypothalamic level by immunofluorescence and serum LH levels were determined by ELISA. The expression of kisspeptin at the hypothalamic level showed sexual dimorphism, where there was an increase in males and a decrease in females during oestrus. There was no change in the expression of GnRH or kisspeptin receptor, regardless of sex. However, a decrease in serum LH concentration was observed in both sexes. The taiep rat showed changes in the expression of kisspeptin at the hypothalamic level. These changes are different from those reported in the literature with the use of animals with experimental allergic encephalomyelitis, this is because both animal models represent different degrees of progression of multiple sclerosis. Our results suggest that the effects on the hypothalamus-pituitary-gonadal axis depend on the differences between the demyelinating processes, their progression, and even individual factors, and it is thus important that fertility treatments are individualized to maximize therapeutic effects.
MeSH term(s)	Animals ; Demyelinating Diseases/metabolism ; Demyelinating Diseases/pathology ; Female ; Gonadotropin-Releasing Hormone/biosynthesis ; Gonadotropin-Releasing Hormone/metabolism ; Hypothalamus/metabolism ; Kisspeptins/biosynthesis ; Luteinizing Hormone/blood ; Male ; Multiple Sclerosis/metabolism ; Multiple Sclerosis/pathology ; Quality of Life ; Rats ; Receptors, Kisspeptin-1/biosynthesis
Chemical Substances	Kiss1r protein, mouse ; Kisspeptins ; Receptors, Kisspeptin-1 ; Gonadotropin-Releasing Hormone (33515-09-2) ; Luteinizing Hormone (9002-67-9)
Language	English
Publishing date	2022-06-16
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639443-7
ISSN	1873-6300 ; 0891-0618
ISSN (online)	1873-6300
ISSN	0891-0618
DOI	10.1016/j.jchemneu.2022.102120
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Article: Near-Infrared Spectroscopy as a Beef Quality Tool to Predict Consumer Acceptance.

Barragán-Hernández, Wilson / Mahecha-Ledesma, Liliana / Angulo-Arizala, Joaquín / Olivera-Angel, Martha

Foods (Basel, Switzerland)

2020 Volume 9, Issue 8

Abstract: This study was conducted to evaluate the feasibility of using near-infrared spectroscopy (NIRS) to predict beef consumers' perceptions. Photographs of 200 raw steaks were taken, and NIRS data were collected (transmittance and reflectance). The steak ... ...

Abstract	This study was conducted to evaluate the feasibility of using near-infrared spectroscopy (NIRS) to predict beef consumers' perceptions. Photographs of 200 raw steaks were taken, and NIRS data were collected (transmittance and reflectance). The steak photographs were used to conduct a face-to-face survey of 400 beef consumers. Consumers rated beef color, visible fat, and overall appearance, using a 5-point Likert scale (where 1 indicated "Dislike very much" and 5 indicated "Like very much"), which later was simplified in a 3-point Likert scale. Factor analysis and structural equation modeling (SEM) were used to generate a beef consumer index. A partial least square discriminant analysis (PLS-DA) was used to predict beef consumers' perceptions using NIRS data. SEM was used to validate the index, with root mean square errors of approximation ≤0.1 and comparative fit and Tucker-Lewis index values <0.9. PLS-DA results for the 5-point Likert scale showed low prediction (accuracy < 42%). A simplified 3-point Likert scale improved discrimination (accuracy between 52% and 55%). The PLS-DA model for purchasing decisions showed acceptable prediction results, particularly for transmittance NIRS (accuracy of 76%). Anticipating beef consumers' willingness to purchase could allow the beef industry to improve products so that they meet consumers' preferences.
Language	English
Publishing date	2020-07-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods9080984
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