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  1. Article ; Online: Ordered deployment of distinct ciliary beating machines in growing axonemes of vertebrate multiciliated cells.

    Lee, Chanjae / Ma, Yun / Tu, Fan / Wallingford, John B

    Differentiation; research in biological diversity

    2023  Volume 131, Page(s) 49–58

    Abstract: The beating of motile cilia requires the coordinated action of diverse machineries that include not only the axonemal dynein arms, but also the central apparatus, the radial spokes, and the microtubule inner proteins. These machines exhibit complex ... ...

    Abstract The beating of motile cilia requires the coordinated action of diverse machineries that include not only the axonemal dynein arms, but also the central apparatus, the radial spokes, and the microtubule inner proteins. These machines exhibit complex radial and proximodistal patterns in mature axonemes, but little is known about the interplay between them during motile ciliogenesis. Here, we describe and quantify the relative rates of axonemal deployment for these diverse cilia beating machineries during the final stages of differentiation of Xenopus epidermal multiciliated cells.
    MeSH term(s) Animals ; Axoneme/metabolism ; Dyneins/metabolism ; Cilia/metabolism ; Vertebrates/metabolism
    Chemical Substances Dyneins (EC 3.6.4.2)
    Language English
    Publishing date 2023-04-13
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 184540-8
    ISSN 1432-0436 ; 0301-4681
    ISSN (online) 1432-0436
    ISSN 0301-4681
    DOI 10.1016/j.diff.2023.03.001
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1170: Show issues Location:
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  2. Article ; Online: Label-free proteomic comparison reveals ciliary and nonciliary phenotypes of IFT-A mutants.

    Leggere, Janelle C / Hibbard, Jaime V K / Papoulas, Ophelia / Lee, Chanjae / Pearson, Chad G / Marcotte, Edward M / Wallingford, John B

    Molecular biology of the cell

    2024  Volume 35, Issue 3, Page(s) ar39

    Abstract: DIFFRAC is a powerful method for systematically comparing proteome content and organization between samples in a high-throughput manner. By subjecting control and experimental protein extracts to native chromatography and quantifying the contents of each ...

    Abstract DIFFRAC is a powerful method for systematically comparing proteome content and organization between samples in a high-throughput manner. By subjecting control and experimental protein extracts to native chromatography and quantifying the contents of each fraction using mass spectrometry, it enables the quantitative detection of alterations to protein complexes and abundances. Here, we applied DIFFRAC to investigate the consequences of genetic loss of Ift122, a subunit of the intraflagellar transport-A (IFT-A) protein complex that plays a vital role in the formation and function of cilia and flagella, on the proteome of
    MeSH term(s) Protein Transport/physiology ; Proteome/metabolism ; Proteomics ; Biological Transport/physiology ; Cilia/metabolism ; Flagella/metabolism ; Phenotype
    Chemical Substances Proteome
    Language English
    Publishing date 2024-01-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1098979-1
    ISSN 1939-4586 ; 1059-1524
    ISSN (online) 1939-4586
    ISSN 1059-1524
    DOI 10.1091/mbc.E23-03-0084
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2853: Show issues Location:
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    Zs.MO 410: Show issues

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  3. Article: Reduction of E. coli O157: H7 and Bacillus cereus levels in red pepper powder using dielectric barrier discharge (DBD) plasma for enhanced quality

    Kim, Yeon Ho / Lee, Chanjae / Lee, Sung-Jae / Yoon, Ki Sun

    Innovative food science & emerging technologies. 2022 Mar., v. 76

    2022  

    Abstract: Although irradiation has been used to destroy bacteria and molds in spices globally, a new technology is needed to alleviate public fears of irradiation, the high cost of irradiated food, and environmental contamination at the irradiation facility. The ... ...

    Abstract Although irradiation has been used to destroy bacteria and molds in spices globally, a new technology is needed to alleviate public fears of irradiation, the high cost of irradiated food, and environmental contamination at the irradiation facility. The objective of this study was to investigate the effects of dielectric barrier discharge (DBD) plasma on Eshcericah coli O157: H7 and Bacillus cereus levels in red pepper powder and the physicochemical properties of red pepper powder stored at 25 °C. RNA expression was determined by qRT-PCR. The surface of bacteria treated with DBD plasma was observed with a Field Emission Scanning Electron Microscope (FE-SEM). Red pepper powder (5 g) inoculated with E. coli O157: H7 and B. cereus was treated with DBD plasma using argon as a plasma-forming gas at 31 kW for 0, 5, 10, and 15 min. DBD plasma did not significantly decrease the bacterial concentrations with an increase in treatment time. However, DBD plasma treatment for 15 min killed E. coli O157: H7 more rapidly during storage. Expression of virulence genes of both E. coli O157: H7 and B. cereus was decreased by DBD plasma treatment for 15 min. Results of FE-SEM showed that DBD plasma damaged the bacterial cell membranes, leading to cell death. Physiochemical properties of red pepper powder were not changed for one month after treatment with DBD plasma. Thus, DBD plasma treatment for 15 min is recommended to control foodborne pathogens in red pepper powder without changing its physicochemical properties.
    Keywords Bacillus cereus ; Escherichia coli O157 ; RNA ; bacteria ; cell death ; food irradiation ; pepper ; pollution ; virulence
    Language English
    Dates of publication 2022-03
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2025029-0
    ISSN 1466-8564
    ISSN 1466-8564
    DOI 10.1016/j.ifset.2022.102916
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    In stock of ZB MED Bonn / Germany

    Z 7327: Show issues

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  4. Article: Active Transposition of Insertion Sequences by Oxidative Stress in

    Lee, Chanjae / Choo, Kyungsil / Lee, Sung-Jae

    Frontiers in microbiology

    2020  Volume 11, Page(s) 558747

    Abstract: Radiation-resistant ... ...

    Abstract Radiation-resistant bacterium
    Language English
    Publishing date 2020-11-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.558747
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  5. Article: Influence of Redox Imbalances on the Transposition of Insertion Sequences in

    Ye, Qianying / Lee, Chanjae / Shin, Eunjung / Lee, Sung-Jae

    Antioxidants (Basel, Switzerland)

    2021  Volume 10, Issue 10

    Abstract: The transposition of insertion sequence elements was evaluated among ... ...

    Abstract The transposition of insertion sequence elements was evaluated among different
    Language English
    Publishing date 2021-10-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox10101623
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  6. Article ; Online: Acquisition of Streptomycin Resistance by Oxidative Stress Induced by Hydrogen Peroxide in Radiation-Resistant Bacterium

    Lee, Chanjae / Ye, Qianying / Shin, Eunjung / Ting, Tian / Lee, Sung-Jae

    International journal of molecular sciences

    2022  Volume 23, Issue 17

    Abstract: Streptomycin is used primarily to treat bacterial infections, including brucellosis, plague, and tuberculosis. Streptomycin resistance easily develops in numerous bacteria through the inhibition of antibiotic transfer, the production of aminoglycoside- ... ...

    Abstract Streptomycin is used primarily to treat bacterial infections, including brucellosis, plague, and tuberculosis. Streptomycin resistance easily develops in numerous bacteria through the inhibition of antibiotic transfer, the production of aminoglycoside-modifying enzymes, or mutations in ribosomal components with clinical doses of streptomycin treatment. (1) Background: A transposable insertion sequence is one of the mutation agents in bacterial genomes under oxidative stress. (2) Methods: In the radiation-resistant bacterium
    MeSH term(s) Anti-Bacterial Agents/metabolism ; Anti-Bacterial Agents/pharmacology ; Deinococcus/genetics ; Deinococcus/metabolism ; Drug Resistance, Bacterial/genetics ; Hydrogen Peroxide/metabolism ; Hydrogen Peroxide/pharmacology ; Mutation ; Oxidative Stress ; Ribosomal Proteins/genetics ; Ribosomal Proteins/metabolism ; Streptomycin/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Ribosomal Proteins ; Hydrogen Peroxide (BBX060AN9V) ; Streptomycin (Y45QSO73OB)
    Language English
    Publishing date 2022-08-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23179764
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  7. Article: Label-free proteomic comparison reveals ciliary and non-ciliary phenotypes of IFT-A mutants.

    Leggere, Janelle C / Hibbard, Jaime V K / Papoulas, Ophelia / Lee, Chanjae / Pearson, Chad G / Marcotte, Edward M / Wallingford, John B

    bioRxiv : the preprint server for biology

    2023  

    Abstract: DIFFRAC is a powerful method for systematically comparing proteome content and organization between samples in a high-throughput manner. By subjecting control and experimental protein extracts to native chromatography and quantifying the contents of each ...

    Abstract DIFFRAC is a powerful method for systematically comparing proteome content and organization between samples in a high-throughput manner. By subjecting control and experimental protein extracts to native chromatography and quantifying the contents of each fraction using mass spectrometry, it enables the quantitative detection of alterations to protein complexes and abundances. Here, we applied DIFFRAC to investigate the consequences of genetic loss of Ift122, a subunit of the intraflagellar transport-A (IFT-A) protein complex that plays a vital role in the formation and function of cilia and flagella, on the proteome of
    Language English
    Publishing date 2023-03-09
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.03.08.531778
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  8. Article: An amino acid-resolution interactome for motile cilia illuminates the structure and function of ciliopathy protein complexes.

    McCafferty, Caitlyn L / Papoulas, Ophelia / Lee, Chanjae / Bui, Khanh Huy / Taylor, David W / Marcotte, Edward M / Wallingford, John B

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Motile cilia are ancient, evolutionarily conserved organelles whose dysfunction underlies motile ciliopathies, a broad class of human diseases. Motile cilia contain myriad different proteins that assemble into an array of distinct machines, so ... ...

    Abstract Motile cilia are ancient, evolutionarily conserved organelles whose dysfunction underlies motile ciliopathies, a broad class of human diseases. Motile cilia contain myriad different proteins that assemble into an array of distinct machines, so understanding the interactions and functional hierarchies among them presents an important challenge. Here, we defined the protein interactome of motile axonemes using cross-linking mass spectrometry (XL/MS) in
    Language English
    Publishing date 2023-09-21
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.07.09.548259
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  9. Article: Transposition of insertion sequences by dielectric barrier discharge plasma and gamma irradiation in the radiation-resistant bacterium Deinococcus geothermalis

    Ye, Qianying / Shin, Eunjung / Lee, Chanjae / Choi, Nakjun / Kim, Yeonho / Yoon, Ki Sun / Lee, Sung-Jae

    Journal of microbiological methods. 2022 Apr. 19,

    2022  

    Abstract: Insertion sequences (ISs) of the radiation-resistant bacterium Deinococcus geothermalis are transposed into other loci by oxidative stress through hydrogen peroxide treatment. Gamma irradiation and dielectric barrier discharge (DBD) plasma radiation are ... ...

    Abstract Insertion sequences (ISs) of the radiation-resistant bacterium Deinococcus geothermalis are transposed into other loci by oxidative stress through hydrogen peroxide treatment. Gamma irradiation and dielectric barrier discharge (DBD) plasma radiation are known to produce a variety of oxidative stress agents such as reactive oxygen species and reactive nitrogen species. Therefore, to determine whether the transposition of ISs was induced in D. geothermalis by both gamma irradiation and DBD plasma radiation, we selected non-pigmented mutants with disrupted target genes encoding carotenoid biosynthesis enzymes such as a phytoene synthase (dgeo_0523) and a phytoene desaturase (dgeo_0524). Different DNA-binding protein-deficient mutants exhibited novel transposition of ISs. Dps (dgeo_0257), OxyR (dgeo_1888), and the LysR (dgeo_2840) family regulator, in addition to cystine importer-disrupted and -overexpressed mutants (dgeo_1986–87 and dgeo_1985R) and wild-type D. geothermalis were tested in this study. Active IS transposition was not detected in two wild-type control species (Deinococcus radiodurans and Deinococcus radiopugnans) after phenotypic selection in gamma irradiation. Our finding demonstrated that gamma irradiation triggers the transposition of particular IS elements, especially ISDge2 and ISDge3 of the IS1 family, ISDge5 of the IS701 family, and ISDge6 of the IS5 family in wild-type strain and the Δdgeo_0257, Δdgeo_1986–87, Δdgeo_1985R, and Δdgeo_2840 mutants. Furthermore, DBD plasma radiation triggered the transposition of ISDge11 of the IS4 family in the wild-type strain; ISDge6 of the IS5 family on Δdgeo_0257, Δdgeo_1888 and Δdgeo_2840; ISDge5 of the IS701 family on Δdgeo_0257 strain.
    Keywords Deinococcus geothermalis ; Deinococcus radiodurans ; Deinococcus radiopugnans ; bacteria ; biosynthesis ; carotenoids ; cystine ; gamma radiation ; hydrogen peroxide ; oxidative stress ; phenotypic selection ; phytoene synthase ; protein deficiencies ; reactive nitrogen species
    Language English
    Dates of publication 2022-0419
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2022.106473
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  10. Article: Influence of Redox Imbalances on the Transposition of Insertion Sequences in Deinococcus geothermalis

    Ye, Qianying / Lee, Chanjae / Shin, Eunjung / Lee, Sung-Jae

    Antioxidants. 2021 Oct. 15, v. 10, no. 10

    2021  

    Abstract: The transposition of insertion sequence elements was evaluated among different Deinococcus geothermalis lineages, including the wild-type, a cystine importer-disrupted mutant, a complemented strain, and a cystine importer-overexpressed strain. Cellular ... ...

    Abstract The transposition of insertion sequence elements was evaluated among different Deinococcus geothermalis lineages, including the wild-type, a cystine importer-disrupted mutant, a complemented strain, and a cystine importer-overexpressed strain. Cellular growth reached early exponential growth at OD₆₀₀ 2.0 and late exponential growth at OD₆₀₀ 4.0. Exposing the cells to hydrogen peroxide (80–100 mM) resulted in the transposition of insertion sequences (ISs) in genes associated with the carotenoid biosynthesis pathway. Particularly, ISDge7 (an IS5 family member) and ISDge5 (an IS701 family member) from the cystine importer-disrupted mutant were transposed into phytoene desaturase (dgeo_0524) via replicative transposition. Further, the cystine importer-overexpressed strain Δdgeo_1985R showed transposition of both ISDge2 and ISDge5 elements. In contrast, IS transposition was not detected in the complementary strain. Interestingly, a cystine importer-overexpressing strain exhibited streptomycin resistance, indicating that point mutation occurred in the rpsL (dgeo_1873) gene encoding ribosomal protein S12. qRT-PCR analyses were then conducted to evaluate the expression of oxidative stress response genes, IS elements, and low-molecular-weight thiol compounds such as mycothiol and bacillithiol. Nevertheless, the mechanisms that trigger IS transposition in redox imbalance conditions remain unclear. Here, we report that the active transposition of different IS elements was affected by intracellular redox imbalances caused by cystine importer deficiencies or overexpression.
    Keywords Deinococcus geothermalis ; biosynthesis ; carotenoids ; cell growth ; cystine ; genes ; hydrogen peroxide ; mutants ; oxidative stress ; point mutation ; ribosomal proteins ; streptomycin ; stress response ; thiols ; transposons
    Language English
    Dates of publication 2021-1015
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox10101623
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