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Article ; Online: Trends and projections in sexually transmitted infections in people aged 45 years and older in England: analysis of national surveillance data.

Camacho, C / Camacho, E M / Lee, D M

2022 Volume 143, Issue 5, Page(s) 263–271

Abstract: Aims: We describe the epidemiology of sexually transmitted infections (STIs) and HIV in people aged ⩾45 years in England and provide future projections about the burden of STIs in this age group.: Methods: Analysis of national surveillance data in ... ...

Abstract	Aims: We describe the epidemiology of sexually transmitted infections (STIs) and HIV in people aged ⩾45 years in England and provide future projections about the burden of STIs in this age group. Methods: Analysis of national surveillance data in England from 2014 to 2019 for chlamydia, gonorrhoea, herpes, syphilis, anogenital warts and HIV was carried out. Time trends were assessed by the Poisson regression and reported using incidence rate ratios (IRRs). Two scenarios were modelled to predict the number of new STI diagnoses and associated costs in 2040. Results: In 2019, there were 37,692 new STI diagnoses in people ⩾45 years in England. Between 2014 and 2019, there was a significant increase in the rate of new STI diagnoses in men (IRR = 1.05, Conclusion: STI rates in England are increasing in people aged ⩾45 years. The population is ageing and older people will contribute an increasing burden to STI costs if this trend continues. The reasons for this trend are not fully understood and further longitudinal epidemiological research is needed. Sexual health promotion campaigns and healthcare interventions targeted at older people should be prioritised.
MeSH term(s)	Male ; Adult ; Humans ; Aged ; Homosexuality, Male ; HIV Infections/epidemiology ; Sexual and Gender Minorities ; Sexually Transmitted Diseases/epidemiology ; England/epidemiology
Language	English
Publishing date	2022-06-29
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2478358-4
ISSN	1757-9147 ; 1757-9139
ISSN (online)	1757-9147
ISSN	1757-9139
DOI	10.1177/17579139221106348
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Article ; Online: Image-Guided Transvaginal and Transabdominal Drain Placement for Surgical Navigation in a Patient with Tubo-Ovarian Abscess and Obstructed Hemivagina and Ipsilateral Renal Anomaly Syndrome.

Parker, P B / Edwards, J R / Bochnakova, T N / Lee, D M

Journal of pediatric and adolescent gynecology

2021 Volume 35, Issue 1, Page(s) 94–97

Abstract: Background: We describe a minimally invasive, image-guided technique for obstructed hemivagina and ipsilateral renal anomaly (OHVIRA) syndrome complicated by tubo-ovarian abscess (TOA).: Case: A 15-year-old female adolescent with OHVIRA syndrome ... ...

Abstract	Background: We describe a minimally invasive, image-guided technique for obstructed hemivagina and ipsilateral renal anomaly (OHVIRA) syndrome complicated by tubo-ovarian abscess (TOA). Case: A 15-year-old female adolescent with OHVIRA syndrome presented with TOA. Magnetic resonance imaging of the abdomen/pelvis showed a loculated pelvic mass and fluid collection near the obstructed left hemivagina. Tissue quality and ill-defined surgical planes prevented surgical excision. A transabdominal drain was placed via laparoscopic port by Interventional Radiology. She later underwent transvaginal and transabdominal drain placement into the hemivaginal collection using computed tomography and ultrasound guidance, allowing for surgical excision of the vaginal septum, drainage and excision of the TOA, and neosalpingostomy. Summary and conclusion: Because of the anatomic complexity in OHVIRA syndrome, preoperative minimally invasive techniques with Interventional Radiology collaboration can assist intraoperative anatomic navigation for successful surgical treatment.
MeSH term(s)	Abscess/diagnostic imaging ; Abscess/surgery ; Adolescent ; Drainage ; Female ; Humans ; Surgery, Computer-Assisted ; Uterus ; Vagina/diagnostic imaging ; Vagina/surgery
Language	English
Publishing date	2021-07-18
Publishing country	United States
Document type	Case Reports
ZDB-ID	1325079-6
ISSN	1873-4332 ; 1083-3188
ISSN (online)	1873-4332
ISSN	1083-3188
DOI	10.1016/j.jpag.2021.07.006
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Article ; Online: Purely Spatial Quantum Diffusion of H Atoms in Solid H_{2} at Temperatures below 1 K.

Sheludiakov, S / Lee, D M / Khmelenko, V V / Järvinen, J / Ahokas, J / Vasiliev, S

Physical review letters

2021 Volume 126, Issue 19, Page(s) 195301

Abstract: We report on a direct measurement of the quantum diffusion of H atoms in solid molecular hydrogen films at T=0.7 K. We obtained a rate of pure spatial diffusion of H atoms in the H_{2} films, D^{d}=5(2)×10^{-17} cm^{2} s^{-1}, which was 2 orders of ... ...

Abstract	We report on a direct measurement of the quantum diffusion of H atoms in solid molecular hydrogen films at T=0.7 K. We obtained a rate of pure spatial diffusion of H atoms in the H_{2} films, D^{d}=5(2)×10^{-17} cm^{2} s^{-1}, which was 2 orders of magnitude faster than that obtained from H atom recombination, the quantity used in all previous work to characterize the mobility of H atoms in solid H_{2}. We also observed that the H-atom diffusion was significantly enhanced by injection of phonons. Our results provide the first measurement of the pure spatial diffusion rate for H atoms in solid H_{2}, the only solid state system beside ^{3}He-^{4}He mixtures, where atomic diffusion does not vanish even at temperatures below 1 K.
Language	English
Publishing date	2021-05-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.126.195301
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Article ; Online: Experimental cell with a Fabry-Pérot resonator tuned in situ for magnetic resonance studies of matrix-isolated radicals at temperatures below 1 K.

Sheludiakov, S / Lee, D M / Khmelenko, V V / Järvinen, J / Ahokas, J / Vasiliev, S

The Review of scientific instruments

2020 Volume 91, Issue 6, Page(s) 63901

Abstract: We describe the design and construction of an experimental cell for the study of free radicals in macroscopically thick films of solidified molecular and rare gases by 128 GHz Electron Spin Resonance (ESR) at temperatures below 1 K. The ESR resonator has ...

Abstract	We describe the design and construction of an experimental cell for the study of free radicals in macroscopically thick films of solidified molecular and rare gases by 128 GHz Electron Spin Resonance (ESR) at temperatures below 1 K. The ESR resonator has an open Fabry-Pérot design, and its frequency can be tuned in situ by adjusting the spacing between the mirrors. The tuning mechanism consists of a piezo positioner and a stainless-steel edge-welded bellows, which can change the resonator frequency by at least 6 GHz. The films of solidified gases can be deposited either directly from a room temperature reservoir or by recondensing from a specially arranged chamber. The free radicals can be created in the solid films by dissociating matrix species by running an rf discharge in a helium vapor. We suggest that such a sample cell design can also be used for a broad range of low-temperature ESR experiments where sample cooling needs to be enhanced by the presence of superfluid helium.
Language	English
Publishing date	2020-06-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	209865-9
ISSN	1089-7623 ; 0034-6748
ISSN (online)	1089-7623
ISSN	0034-6748
DOI	10.1063/5.0012178
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Article ; Online: Endoplasmic reticulum stress and the development of endothelial dysfunction.

Battson, M L / Lee, D M / Gentile, C L

American journal of physiology. Heart and circulatory physiology

2016 Volume 312, Issue 3, Page(s) H355–H367

Abstract: The vascular endothelium plays a critical role in cardiovascular homeostasis, and thus identifying the underlying causes of endothelial dysfunction has important clinical implications. In this regard, the endoplasmic reticulum (ER) has recently emerged ... ...

Abstract	The vascular endothelium plays a critical role in cardiovascular homeostasis, and thus identifying the underlying causes of endothelial dysfunction has important clinical implications. In this regard, the endoplasmic reticulum (ER) has recently emerged as an important regulator of metabolic processes. Dysfunction within the ER, broadly termed ER stress, evokes the unfolded protein response (UPR), an adaptive pathway that aims to restore ER homeostasis. Although the UPR is the first line of defense against ER stress, chronic activation of the UPR leads to cell dysfunction and death and has recently been implicated in the pathogenesis of endothelial dysfunction. Numerous risk factors for endothelial dysfunction can induce ER stress, which may in turn disrupt endothelial function via direct effects on endothelium-derived vasoactive substances or by activating other pathogenic cellular networks such as inflammation and oxidative stress. This review summarizes the available data linking ER stress to endothelial dysfunction.
MeSH term(s)	Animals ; Endoplasmic Reticulum ; Endoplasmic Reticulum Stress ; Endothelium, Vascular/physiopathology ; Humans ; Unfolded Protein Response ; Vascular Diseases/physiopathology
Language	English
Publishing date	2016-12-06
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	603838-4
ISSN	1522-1539 ; 0363-6135
ISSN (online)	1522-1539
ISSN	0363-6135
DOI	10.1152/ajpheart.00437.2016
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Article ; Online: Intravenous neutralization of vascular endothelial growth factor reduces vascular function/permeability of the ovary and prevents development of OHSS-like symptoms in rhesus monkeys.

Bishop, C V / Lee, D M / Slayden, O D / Li, X

Journal of ovarian research

2017 Volume 10, Issue 1, Page(s) 41

Abstract: Background: Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with elevated serum VEGFA following chorionic gonadotropin (hCG) exposure in controlled ovarian stimulation (COS) cycles in women. In this study, we tested the effect of ... ...

Abstract	Background: Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with elevated serum VEGFA following chorionic gonadotropin (hCG) exposure in controlled ovarian stimulation (COS) cycles in women. In this study, we tested the effect of intravenous VEGFA neutralization on OHSS-like symptoms and vascular function in rhesus macaques during COS cycles. Methods: Monkeys (n = 8) were treated with 3 COS protocols and assigned randomly to groups as follows: 1) COS alone (Control, n = 5); 2) COS + VEGF mAb Avastin 19 ± 5 h before hCG (Avastin pre-hCG; n = 6); 3) COS + Avastin 3-4 days post-hCG (Avastin post-hCG; n = 4); 4) COS + Simulated Early Pregnancy (SEP n = 3); or 5) COS + SEP + Avastin (SEP + Avastin n = 3). Follicles were aspirated 36 h post-hCG, fluid was collected from one follicle for analysis of steroid and vascular hormone content. Remaining follicles were aspirated, and luteinized granulosa cells (LGCs) cultured for 24 h. Ovarian/uterine vascular flow (VF) and blood volume (BV) were analyzed by contrast enhanced ultrasound (CEUS) before hCG bolus and 6-8 days post-hCG bolus/time of peak SEP response. Ovarian permeability to albumin was analyzed by Dynamic Contrast Enhanced-MRI (DCE-MRI) post-hCG. Results: Abdominal fluid was present in 4/5 Control, 2/6 Avastin pre-hCG, and 3/4 Avastin post-hCG females. Neutralization of VEGFA before hCG reduced ovarian VF, BV, and permeability to albumin (P < 0.05), while only ovarian VF and permeability were reduced in Avastin-post hCG group (P < 0.05). There was no effect of Avastin on ovarian vascular function during COS + SEP. VEGF levels in follicular fluid were reduced 78-fold by Avastin pre-hCG, and LGCs exposed to Avastin in vivo also released 4-fold less VEGF into culture media (P < 0.05). Culture medium of LGCs exposed to VEGFA neutralization in vivo had lower levels of P4 and ANGPT1, and an increased ratio of ANGPT2/1 (P < 0.05). Uterine VF was reduced by SEP + Avastin in the basalis/junctional zone (P < 0.05). Conclusions: Avastin treatment before hCG prevents the development of symptoms associated with ovarian hyperstimulation syndrome. In vitro data suggest neutralization of VEGFA alters expression of other vascular factors typically induced by hCG in the luteinizing follicle. Neutralization of VEGFA action alters the vascular function of the basalis zone of the uterus during simulated early pregnancy, indicating a potential effect on embryo implantation.
MeSH term(s)	Angiogenesis Inhibitors/administration & dosage ; Animals ; Bevacizumab/administration & dosage ; Capillary Permeability/drug effects ; Chorionic Gonadotropin/adverse effects ; Contrast Media ; Female ; Humans ; Image Enhancement ; Macaca mulatta ; Magnetic Resonance Imaging ; Menstrual Cycle ; Ovarian Follicle/drug effects ; Ovarian Follicle/metabolism ; Ovarian Hyperstimulation Syndrome/diagnostic imaging ; Ovarian Hyperstimulation Syndrome/metabolism ; Ovarian Hyperstimulation Syndrome/pathology ; Ovarian Hyperstimulation Syndrome/prevention & control ; Ovary/drug effects ; Ovary/metabolism ; Ovulation Induction ; Pregnancy ; Vascular Endothelial Growth Factor A/antagonists & inhibitors
Chemical Substances	Angiogenesis Inhibitors ; Chorionic Gonadotropin ; Contrast Media ; Vascular Endothelial Growth Factor A ; Bevacizumab (2S9ZZM9Q9V)
Language	English
Publishing date	2017-07-06
Publishing country	England
Document type	Journal Article
ZDB-ID	2455679-8
ISSN	1757-2215 ; 1757-2215
ISSN (online)	1757-2215
ISSN	1757-2215
DOI	10.1186/s13048-017-0340-5
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Article ; Online: Erectile dysfunction and phosphodiesterase type 5 inhibitor use: associations with sexual activities, function and satisfaction in a population sample of older men.

Lee, D M / Nazroo, J / Pendleton, N

International journal of impotence research

2015 Volume 27, Issue 4, Page(s) 146–151

Abstract: The objective of this study was to examine the association between sexual activities, problems and satisfaction, and ED and PDE5 inhibitor (PDE5i) use. A nationally representative sample of men (n=2612) aged 51-87 years from the English Longitudinal ... ...

Abstract	The objective of this study was to examine the association between sexual activities, problems and satisfaction, and ED and PDE5 inhibitor (PDE5i) use. A nationally representative sample of men (n=2612) aged 51-87 years from the English Longitudinal Study of Ageing completed an in-depth Sexual Relationships and Activities Questionnaire. Associations between ED and/or PDE5i use and sexual outcomes were explored using logistic regression models adjusted for age, health and lifestyle factors. PDE5i use in the preceding 3 months was reported by a total of 191 (7%) men, whereas 542 (21%) reported ED but no PDE5i use (untreated ED). Compared with men without ED, PDE5i users were more likely to be sexually active and report more frequent sexual intercourse. Men with untreated ED reported the lowest frequency of sexual activities. Compared with men without ED, both PDE5i users and those with untreated ED were more likely to report being concerned about their level of sexual desire, frequency of sexual activities, erectile function, waking erections and orgasmic experience. PDE5i users were also more concerned about and dissatisfied with their overall sex life than men without ED. This population-based study shows that while PDE5i use is associated with improved sexual functioning, this is not equally reflected in decreased levels of concern and dissatisfaction with their overall sexual health. Clinicians should be aware of this disparity between functional gains and continuing sexual concerns and dissatisfaction, and, where appropriate, offer psychosexual counselling as an adjunct to PDE5i medication.
MeSH term(s)	Aged ; Cyclic Nucleotide Phosphodiesterases, Type 5/drug effects ; Cyclic Nucleotide Phosphodiesterases, Type 5/metabolism ; Drug Utilization ; Erectile Dysfunction/drug therapy ; Erectile Dysfunction/epidemiology ; Erectile Dysfunction/psychology ; Female ; Health Surveys ; Humans ; Male ; Middle Aged ; Patient Satisfaction ; Phosphodiesterase Inhibitors/therapeutic use ; Sexual Behavior ; Sexual Dysfunction, Physiological/drug therapy ; Sexual Dysfunction, Physiological/epidemiology ; Sexual Dysfunction, Physiological/psychology ; Sexuality/drug effects ; Surveys and Questionnaires ; United Kingdom/epidemiology
Chemical Substances	Phosphodiesterase Inhibitors ; Cyclic Nucleotide Phosphodiesterases, Type 5 (EC 3.1.4.35)
Language	English
Publishing date	2015-03-26
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1034295-3
ISSN	1476-5489 ; 0955-9930
ISSN (online)	1476-5489
ISSN	0955-9930
DOI	10.1038/ijir.2015.4
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Zs.A 2910: Show issues

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Article ; Online: Salivary Testosterone and Sexual Function and Behavior in Men and Women: Findings from the Third British National Survey of Sexual Attitudes and Lifestyles (Natsal-3).

Macdowall, W G / Clifton, S / Palmer, M J / Tanton, C / Copas, A J / Lee, D M / Mitchell, K R / Mercer, C H / Sonnenberg, P / Johnson, A M / Wellings, K

Journal of sex research

2021 Volume 59, Issue 2, Page(s) 135–149

Abstract: Using data from the third British National Survey of Sexual Attitudes and Lifestyles (Natsal-3) we examined associations between salivary testosterone (Sal-T) and sexual function and behavior. Single morning saliva samples were self-collected from a ... ...

Abstract	Using data from the third British National Survey of Sexual Attitudes and Lifestyles (Natsal-3) we examined associations between salivary testosterone (Sal-T) and sexual function and behavior. Single morning saliva samples were self-collected from a subsample of participants aged 18-74 years and analyzed using mass spectrometry. 1,599 men and 2,123 women were included in the analysis (40.6% of those invited to provide a sample). We adjusted for confounders in a stepwise manner: in model 1 we adjusted for age only; model 2 for age, season and relationship status, and model 3 we added BMI and self-reported health. In the fully adjusted models, among men, Sal-T was positively associated with both partnered sex (vaginal sex and concurrent partners) and masturbation. Among women, Sal-T was positively associated with masturbation, the only association with partnered sex was with ever experience of same-sex sex. We found no clear association between Sal-T and sexual function. Our study contributes toward addressing the sparsity of data outside the laboratory on the differences between men and women in the relationship between T and sexual function and behavior. To our knowledge, this is the first population study, among men and women, using a mass spectrometry Sal-T assay to do so.
MeSH term(s)	Attitude ; Female ; Health Surveys ; Humans ; Life Style ; Male ; Sexual Behavior ; Sexual Partners ; Testosterone ; United Kingdom/epidemiology
Chemical Substances	Testosterone (3XMK78S47O)
Language	English
Publishing date	2021-10-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	220889-1
ISSN	1559-8519 ; 0022-4499
ISSN (online)	1559-8519
ISSN	0022-4499
DOI	10.1080/00224499.2021.1968327
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Article ; Online: Formation of Nuclear-Polarized Phases of H Atoms Embedded in Solid H_{2} Films.

Sheludiakov, S / McColgan, P T / Lee, D M / Khmelenko, V V / Järvinen, J / Ahokas, J / Vasiliev, S

Physical review letters

2019 Volume 122, Issue 22, Page(s) 225301

Abstract: We report on an experimental observation of two phases of hydrogen atoms in solid H_{2} films at temperatures of 0.1-0.8 K, characterized by a large enhancement of the nuclear spin polarization compared to that given by Boltzmann statistics (p=0.15 at T= ... ...

Abstract	We report on an experimental observation of two phases of hydrogen atoms in solid H_{2} films at temperatures of 0.1-0.8 K, characterized by a large enhancement of the nuclear spin polarization compared to that given by Boltzmann statistics (p=0.15 at T=0.15 K). The first phase with p=0.35(5) is formed spontaneously during sample storage in a high magnetic field (B=4.6 T). The second phase with an even higher nuclear polarization, p=0.75(7), can be achieved at T≤0.55 K by repeating sequences of dynamic nuclear polarization followed by a system relaxation. Upon warming through the range 0.55-0.65 K, the highly nuclear-polarized phase undergoes a phase transition to the spontaneously polarized phase which breaks down at T≃0.8 K, and the nuclear polarization gradually converges to the Boltzmann distribution. We discuss possible scenarios for explaining the nature of the observed phenomena.
Language	English
Publishing date	2019-07-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.122.225301
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Article: A simple and sensitive method in using the ratios of cholesteryl ester molecular species as indexes of oxidative stress in plasma and lipoprotein fractions.

Lee, D M

Atherosclerosis

1999 Volume 146, Issue 2, Page(s) 221–235

Abstract: To develop a simpler method for lipid peroxidation which may replace the classic gold standard of measuring the loss of polyunsaturated fatty acids, we investigated the use of ratios of molecular species of neutral lipids (MSNL) separated by gas ... ...

Abstract	To develop a simpler method for lipid peroxidation which may replace the classic gold standard of measuring the loss of polyunsaturated fatty acids, we investigated the use of ratios of molecular species of neutral lipids (MSNL) separated by gas chromatography in a single step. We sub-fractionated lipoproteins and subjected each to oxidation. Among different combinations of ratios of MSNL, we found that the ratios of cholesteryl esters (CE) containing C20/C16 (R1) and C18/C16 (R2) decreased most rapidly with increasing Cu2+ concentration and with increasing oxidation time, for all lipoproteins. We suggest that these two CE ratios can be used as oxidative indexes for all lipoproteins and thus for intact plasma. Experimental evidence showed that the oxidative index of whole plasma is the weighted average index of its lipoproteins. This method was validated with thiobarbituric acid method. Normal healthy asymptomatic males had R1 value 0.496 +/- 0.130, and R2 4.674 +/- 0.848, and females had R1 0.535 +/- 0.117 and R2 4.569 +/- 0.733 with no significant gender differences. Both ratios showed low variabilities within each individual. The method was tested to be feasible in monitoring vitamin E supplementation study. This CE-ratio method is concentration independent. It is simple, rapid, and highly reproducible, and, suitable for screening plasma on large scale.
MeSH term(s)	Cholesterol Esters/analysis ; Chromatography, Gas ; Copper/pharmacology ; Female ; Humans ; Lipid Peroxidation/drug effects ; Lipid Peroxidation/physiology ; Lipoproteins, HDL/chemistry ; Lipoproteins, LDL/chemistry ; Male ; Oxidative Stress/drug effects ; Oxidative Stress/physiology ; Plasma/chemistry ; Reference Values ; Reproducibility of Results ; Thiobarbituric Acid Reactive Substances/pharmacology ; Triglycerides/blood ; Vitamin E/pharmacology
Chemical Substances	Cholesterol Esters ; Lipoproteins, HDL ; Lipoproteins, LDL ; Thiobarbituric Acid Reactive Substances ; Triglycerides ; Vitamin E (1406-18-4) ; Copper (789U1901C5)
Language	English
Publishing date	1999-10
Publishing country	Ireland
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80061-2
ISSN	1879-1484 ; 0021-9150
ISSN (online)	1879-1484
ISSN	0021-9150
DOI	10.1016/s0021-9150(99)00136-7
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