Article ; Online: Urechistachykinin I triggers mitochondrial dysfunction leading to a ferroptosis-like response in Saccharomyces cerevisiae.
Journal of applied microbiology
2024 Volume 135, Issue 3
Abstract: Aims: The purpose of this paper was to demonstrate the antimicrobial activity of urechistachykinin I (LRQSQFVGSR-NH2) extracted from Urechis unicinctus,and its mode of action dependent on mitochondrial dysfunction.: Methods and results: The ... ...
Abstract | Aims: The purpose of this paper was to demonstrate the antimicrobial activity of urechistachykinin I (LRQSQFVGSR-NH2) extracted from Urechis unicinctus,and its mode of action dependent on mitochondrial dysfunction. Methods and results: The antifungal activity of urechistachykinin I generated reactive oxygen species (ROS), as demonstrated with MitoSOX Red and hydroxyphenyl fluorescein (HPF). Overaccumulation of ROS caused oxidative damage to cells by inducing mitochondrial dysfunction. Mitochondrial disruption resulted in cell death, creating several hallmarks that included lipid peroxidation, glutathione oxidation, and depolarization. Moreover, the loss of mitochondria changed the calcium ion imbalance by depolarization of the mitochondrial membrane. In particular, iron accumulation and DNA fragmentation measurement determined the type of cell death. Our results indicate that urechistachykinin I treatment induced ferroptosis-like death in Saccharomyces cerevisiae via mitochondrial dysfunction. Conclusions: Urechistachykinin I treatment induced mitochondrial dysfunction in S. cerevisiae by generating ROS, and the subsequent oxidative damage caused the ferroptosis-like cell death. |
---|---|
MeSH term(s) | Humans ; Saccharomyces cerevisiae/metabolism ; Reactive Oxygen Species/metabolism ; Ferroptosis ; Mitochondrial Diseases ; Neuropeptides |
Chemical Substances | Reactive Oxygen Species ; urechistachykinin I (149097-03-0) ; Neuropeptides |
Language | English |
Publishing date | 2024-01-24 |
Publishing country | England |
Document type | Journal Article |
ZDB-ID | 1358023-1 |
ISSN | 1365-2672 ; 1364-5072 |
ISSN (online) | 1365-2672 |
ISSN | 1364-5072 |
DOI | 10.1093/jambio/lxae011 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
Zs.A 259: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.