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  1. Article: Characterization of bio-adsorptive removal performance of strontium through ureolysis-mediated bio-mineralization

    Kim, Hayeon / Son, H.M. / Lee, H.K.

    Chemosphere. 2022 Feb., v. 288

    2022  

    Abstract: The adsorptive removal performance of strontium (Sr) through bio-mineralization metabolism under various parameters was evaluated in this study. The primary mechanism of bio-mineralization used in this study was the urea hydrolysis process through ... ...

    Abstract The adsorptive removal performance of strontium (Sr) through bio-mineralization metabolism under various parameters was evaluated in this study. The primary mechanism of bio-mineralization used in this study was the urea hydrolysis process through bacterial enzymatic catalysis. Bacillus sp, which was isolated from river sediment, was used as a ureolytic bacteria. Various environmental conditions were set as different initial concentrations of Sr (10, 50, 100, 200, and 500 mg/L), and various ratios of Mg/Ca (4, 2, 1, 0.5, and 0.25). The concentrations of Sr²⁺, Ca²⁺, and Mg²⁺ in the solution of the batch experiment were measured to identify the bio-mineralization performance and the removal rate of Sr. In addition, the main Sr removal mechanism of ureolytic bacteria was identified. As a result, for Sr removal of bacteria, the bio-mineralization mechanism was more predominant than the adsorption of Sr. The rapid growth and high nucleation site production were observed when the initial concentration of Sr²⁺ increased and the Mg/Ca ratio was lowered, resulting in high biomineralization performance and Sr removal rate. The main phases of carbonate minerals formed in the presence of Sr, Ca, and Mg were SrCO₃ and SrCa(CO₃)₂. Mg²⁺ could retard the bacterial growth and participate in the formation of carbonate minerals, when a large amount of Mg²⁺ was present. Furthermore, the desorption rate of Sr²⁺ from bacterial pastes containing the carbonate minerals increased as the concentration of HCl increased, although the carbonate minerals were in a stable state.
    Keywords Bacillus (bacteria) ; adsorption ; bacterial growth ; biomineralization ; calcium ; catalytic activity ; desorption ; hydrolysis ; metabolism ; rivers ; sediments ; strontium ; urea
    Language English
    Dates of publication 2022-02
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2021.132586
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Complete genome sequence of seed-transmitted soybean yellow mottle mosaic virus from soybeans in Korea.

    Bak, Sangmin / Kwon, Mina / Baek, Seungbin / Yang, Minjoo / Lee, Hong-Kyu / Lee, Su-Heon

    Microbiology resource announcements

    2023  Volume 12, Issue 11, Page(s) e0083723

    Abstract: Soybean yellow mottle mosaic virus (SYMMV), a member of the ... ...

    Abstract Soybean yellow mottle mosaic virus (SYMMV), a member of the genus
    Language English
    Publishing date 2023-10-31
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00837-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Characterization of bio-adsorptive removal performance of strontium through ureolysis-mediated bio-mineralization.

    Kim, Hayeon / Son, H M / Lee, H K

    Chemosphere

    2021  Volume 288, Issue Pt 2, Page(s) 132586

    Abstract: The adsorptive removal performance of strontium (Sr) through bio-mineralization metabolism under various parameters was evaluated in this study. The primary mechanism of bio-mineralization used in this study was the urea hydrolysis process through ... ...

    Abstract The adsorptive removal performance of strontium (Sr) through bio-mineralization metabolism under various parameters was evaluated in this study. The primary mechanism of bio-mineralization used in this study was the urea hydrolysis process through bacterial enzymatic catalysis. Bacillus sp, which was isolated from river sediment, was used as a ureolytic bacteria. Various environmental conditions were set as different initial concentrations of Sr (10, 50, 100, 200, and 500 mg/L), and various ratios of Mg/Ca (4, 2, 1, 0.5, and 0.25). The concentrations of Sr
    MeSH term(s) Adsorption ; Strontium
    Chemical Substances Strontium (YZS2RPE8LE)
    Language English
    Publishing date 2021-10-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2021.132586
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Complete genome sequence of soybean geminivirus A in soybean in Korea.

    Bak, Sangmin / Kwon, Mina / Baek, Seungbin / Min, Jean Geung / Kang, Dong Hyun / Kim, Minseok / Lee, Hong-Kyu / Lee, Su-Heon

    Microbiology resource announcements

    2023  Volume 12, Issue 10, Page(s) e0055323

    Abstract: Soybean geminivirus A (SGVA), a member of the family Geminiviridae, was detected in a survey of early-stage soybean. The complete genome sequence of SGVA isolate Habin was determined, revealing its characteristics and similarity to Korean and Chinese ... ...

    Abstract Soybean geminivirus A (SGVA), a member of the family Geminiviridae, was detected in a survey of early-stage soybean. The complete genome sequence of SGVA isolate Habin was determined, revealing its characteristics and similarity to Korean and Chinese isolates. This study contributes to understanding the impact of SGVA on soybean production.
    Language English
    Publishing date 2023-09-27
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00553-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: A PCR-Based Assay to Detect Rhynchosporium secalis in Barley Seed.

    Lee, H K / Tewari, J P / Turkington, T K

    Plant disease

    2019  Volume 85, Issue 2, Page(s) 220–225

    Abstract: A polymerase chain reaction (PCR)-based diagnostic assay was developed to detect Rhynchosporium secalis, the barley scald fungus, in barley seed. Species-specific primers were designed based on sequence data of a region consisting of the 5.8S RNA gene ... ...

    Abstract A polymerase chain reaction (PCR)-based diagnostic assay was developed to detect Rhynchosporium secalis, the barley scald fungus, in barley seed. Species-specific primers were designed based on sequence data of a region consisting of the 5.8S RNA gene and internal transcribed spacers 1 and 2 of R. secalis. The sequenced regions showed 100% homology between the two R. secalis isolates and 93% homology between R. secalis and R. orthosporum. Five sets of synthesized oligonucleotide primers were tested for their specificity using 29 isolates of R. secalis of diverse geographic origins and from different barley cultivars. In addition, DNA extracts from 22 species of microbes either taxonomically related to or from the same niche as R. secalis were tested as negative controls. Among five sets of primers, a primer set, RS8 and RS9, was selected for use in detecting R. secalis because it amplified a 264-bp fragment from the DNA of all R. secalis isolates but not the DNA from other species used for validation of the specificity of this primer set. This primer set was also used to detect R. secalis in barley seed and successfully amplified the predicted size of the DNA fragment in the infected material. PCR detection of as little as 1 to 10 pg of R. secalis DNA was possible. The method described here requires 1 day for completion, compared to 10 days required for the cultural method.
    Language English
    Publishing date 2019-03-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS.2001.85.2.220
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: First Report of Olive Mild Mosaic Virus in Imported Tulips (Tulipa gesneriana) in Korea

    Bak, S. / Kim, M. P. / Kim, H. J. / Kang, E.-H. / Kang, D. H. / Min, J. G. / Han, Siyuan / Lee, H. K. / Lee, S. H.

    Plant Disease. 2023 Sept. 01, v. 107, no. 9 p.2895-

    2023  

    Abstract: Tulip cultivation in Korea primarily uses imported bulbs because of the absence of domestic production. To ensure safety and sustainability, Korean authorities have implemented strict phytosanitary measures for five viruses: arabis mosaic virus, tobacco ... ...

    Abstract Tulip cultivation in Korea primarily uses imported bulbs because of the absence of domestic production. To ensure safety and sustainability, Korean authorities have implemented strict phytosanitary measures for five viruses: arabis mosaic virus, tobacco necrosis virus, tobacco ringspot virus, tomato black ring virus, and tomato bushy stunt virus. In April 2021, 86 tulip plants presented symptoms such as chlorotic mottle, mosaic, streak, stripe, yellowing of the leaves, and color breaking on flowers. These samples were collected to investigate the incidence of viruses in four Korean provinces (Gangwon, Gyeongbuk, Gyeongnam, and Chungnam). The leaves and petals from each individual sample (10 mg each) were pooled and ground using liquid nitrogen. Total RNA was extracted using a Maxwell 16 LEV Plant RNA Kit (Promega, Madison, WI, U.S.A.). A cDNA library was constructed using TruSeq Stranded Total RNA with Ribo-Zero (Illumina, San Diego, CA, U.S.A.) and sequenced on an Illumina NovaSeq 6000 platform (Macrogen, Seoul, Korea) with 100-bp paired-end reads. Trinity software identified tulip breaking virus (TBV), tulip virus X, and lily symptomless virus (LSV), which are known to occur in Korea (Bak et al. 2023), by de novo assembly of 628 million reads into 498,795 contigs. The contigs were annotated as previously described (Bak et al. 2022). Moreover, a contig (ON758350) related to olive mild mosaic virus (OMMV; genus Alphanecrovirus, family Tombusviridae) was identified through BLASTn analysis. This contig had 99.27% nucleotide (nt) identity to OMMV PPO-L190209 (KU641010), which was assembled from 201,346 reads and spanned 3,713 bp. To confirm the presence of OMMV, a primer pair (5′-GAATGTCTGGCGTTAAGCG-3′/5′-GTGTCCTGCGCATCATACAC-3′) was designed to amplify a 797-bp fragment of the coat protein gene. In RT-PCR, 31.4% (27/86) of samples were positive for OMMV and coinfected with TBV or TBV + LSV. Coinfection with TBV led to chlorotic mottling and stripes, whereas triple coinfection with TBV + LSV produced distinct yellow streaks and mosaic within the lesion boundaries. In contrast, solely TBV infection did not produce such symptoms. The samples infected with OMMV were exclusively collected from Gangwon and Gyeongnam. In each province, an RT-PCR amplicon was cloned and subsequently sequenced (Bioneer, Daejeon, Korea). The obtained sequences were named CC (OM243091) and GS (OM243092), and they shared 98.6 and 98.9% identity with PPO-L190209 (KU641010), respectively. A bioassay was conducted using a leaf infected with OMMV CC and TBV to inoculate 13 indicator species in triplicate, including Capsicum annuum, Chenopodium amaranticolor, C. quinoa, Cucumis sativus, Nicotiana benthamiana, N. clevelandii, N. glutinosa, N. occidentalis, N. rustica, N. tabacum, Solanum lycopersicum, Tetragonia tetragonioides, and Tulipa gesneriana. The RT-PCR revealed positivity only for OMMV in the upper leaves of N. clevelandii, whereas all other species were negative with no symptoms. To our knowledge, this is the first report of OMMV occurring in tulips grown from imported bulbs in Korea, with no other known natural hosts such as olive tree (Cardoso et al. 2004), spinach (Gratsia et al. 2012), and corn salad (Verdin et al. 2018). The Korean OMMV isolates exhibited high nt identity with the foreign isolate, and the samples were collected from farms that rely entirely on bulb imports for cultivation. These suggest that the outbreak of OMMV was likely caused by imported bulbs.
    Keywords Arabis mosaic virus ; Capsicum annuum ; Chenopodium giganteum ; Cucumis sativus ; Lily symptomless virus ; Nicotiana benthamiana ; Olea europaea ; Olive mild mosaic virus ; RNA ; Solanum lycopersicum ; Tetragonia tetragonoides ; Tobacco necrosis virus ; Tobacco ringspot virus ; Tomato black ring virus ; Tomato bushy stunt virus ; Tulipa gesneriana ; Valerianella locusta ; bioassays ; bulbs ; cDNA libraries ; coat proteins ; color ; computer software ; genes ; indicator species ; leaves ; liquid nitrogen ; mixed infection ; oligodeoxyribonucleotides ; spinach ; viruses ; Korean Peninsula ; Korea ; virus
    Language English
    Dates of publication 2023-0901
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-03-23-0527-PDN
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Impact of Bio-Carrier Immobilized with Marine Bacteria on Self-Healing Performance of Cement-Based Materials.

    Kim, Hayeon / Son, Hyeongmin / Seo, Joonho / Lee, H K

    Materials (Basel, Switzerland)

    2020  Volume 13, Issue 18

    Abstract: The present study evaluated the self-healing efficiency and mechanical properties of mortar specimens incorporating a bio-carrier as a self-healing agent. The bio-carrier was produced by immobilizing ureolytic bacteria isolated from seawater in bottom ... ...

    Abstract The present study evaluated the self-healing efficiency and mechanical properties of mortar specimens incorporating a bio-carrier as a self-healing agent. The bio-carrier was produced by immobilizing ureolytic bacteria isolated from seawater in bottom ash, followed by surface coating with cement powder to prevent loss of nutrients during the mixing process. Five types of specimens were prepared with two methods of incorporating bacteria, and were water cured for 28 days. To investigate the healing ratio, the specimens with predefined cracks were treated by applying a wet-dry cycle in three different conditions, i.e., seawater, tap water, and air for 28 days. In addition, a compression test and a mercury intrusion porosimetry analysis of the specimens were performed to evaluate their physico-mechanical properties. The obtained results showed that the specimen incorporating the bio-carrier had higher compressive strength than the specimen incorporating vegetative cells. Furthermore, the highest healing ratio was observed in specimens incorporating the bio-carrier. This phenomenon could be ascribed by the enhanced bacterial viability by the bio-carrier.
    Language English
    Publishing date 2020-09-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma13184164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Facile Synthesis of Sprayed CNTs Layer-Embedded Stretchable Sensors with Controllable Sensitivity.

    Khalid, Hammad R / Choudhry, Iqra / Jang, Daeik / Abbas, Nadir / Haider, M Salman / Lee, H K

    Polymers

    2021  Volume 13, Issue 2

    Abstract: Flexible electronic devices have gained significant interest due to their different potential applications. Herein, we report highly flexible, stretchable, and sensitive sensors made of sprayed CNT layer, sandwiched between two polymer layers. A facile ... ...

    Abstract Flexible electronic devices have gained significant interest due to their different potential applications. Herein, we report highly flexible, stretchable, and sensitive sensors made of sprayed CNT layer, sandwiched between two polymer layers. A facile fabrication process was employed in which the CNT solution was directly sprayed onto a patterned bottom polymer layer, above which a second polymer layer was casted to get a sandwiched composite structure. Varying amounts of CNT solution (i.e., 10, 25, 40, 70, and 100 mL) were sprayed to get conductive CNT layers of different thicknesses/densities. The physical characteristics of the conductive CNT layers were studied through SEM and optical images. The starting electrical resistance values (without strain) as well as the changes in electrical resistance against human body motions were monitored. The synthesized samples exhibited good response against finger and wrist bending. The conductivity of the samples increased with increase of CNT solution volume while the sensitivity followed the inverse relation, suggesting that the sensors with controlled sensitivity could be fabricated for targeted strain ranges using the proposed method.
    Language English
    Publishing date 2021-01-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym13020311
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: A method of evaluating facial pores using optical 2D images and analysis of age-dependent changes in facial pores in Koreans.

    Jang, S I / Kim, E J / Lee, H K

    Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

    2018  Volume 24, Issue 2, Page(s) 304–308

    Abstract: Background: Enlarged facial pores and changes in pore area are of concern for cosmetic reasons. To evaluate pores, measuring tools based on 3D methodology are used. Yet, these methods are limited by their measuring ranges. In this study, we performed ... ...

    Abstract Background: Enlarged facial pores and changes in pore area are of concern for cosmetic reasons. To evaluate pores, measuring tools based on 3D methodology are used. Yet, these methods are limited by their measuring ranges. In this study, we performed pore analysis by measuring the whole face using 2D optical images. We further sought to understand how the pores of Korean women change with age.
    Materials and methods: One hundred sixteen Korean female subjects aged 20-60 years were recruited for this study. Facial images were taken using the VISIA-CR
    Results: The pore area, as indicated by pixel count, gradually increased in patients through their 40s, but decreased through their 50s and 60s. Facial pores generally exhibited directionality through the patients' 30s, but this isotropic feature was more prominent in their 50s. Pore elongation increased stepwise. The first increase occurred during the transition from patients' 30s to their 40s and the second increase occurred during the transition from patients' 50s to their 60s. This indicated that the pores deformed from a circular shape to a long elliptic shape over time.
    Conclusion: A new evaluation method using 2D optical images facilitates the analysis of pore distribution and elongation throughout the entire cheek. This is an improvement over an analysis of pores over a narrow region of interest.
    MeSH term(s) Adult ; Age Distribution ; Face ; Female ; Humans ; Middle Aged ; Republic of Korea/ethnology ; Sebaceous Glands/anatomy & histology ; Skin/anatomy & histology ; Skin Aging/ethnology ; Skin Aging/pathology ; Young Adult
    Language English
    Publishing date 2018-01-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 1229160-2
    ISSN 1600-0846 ; 0909-752X ; 1397-1344
    ISSN (online) 1600-0846
    ISSN 0909-752X ; 1397-1344
    DOI 10.1111/srt.12430
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Use of SkinFibrometer

    Kim, M A / Kim, E J / Lee, H K

    Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

    2018  Volume 24, Issue 3, Page(s) 466–471

    Abstract: Background: Skin elasticity is an important indicator of skin aging. The aim of this study was to demonstrate that the SkinFibrometer: Materials and methods: Twenty-one individuals participated in this study. The skin of the cheek, around the eye, ... ...

    Abstract Background: Skin elasticity is an important indicator of skin aging. The aim of this study was to demonstrate that the SkinFibrometer
    Materials and methods: Twenty-one individuals participated in this study. The skin of the cheek, around the eye, and the volar forearm were evaluated. To analyze correlations of elasticity parameters, the induration value against the indenter pressure of SkinFibrometer
    Results: The younger age group showed more firm and elastic skin properties compared to the older age group, and the elasticity values of the volar forearm were significantly higher than those of the cheek and around the eye region. Even though the measuring principle is different, both SkinFibrometer
    Conclusion: We identified correlations between skin elasticity parameters evaluated by two different methods of suction and indentation, and demonstrated that the SkinFibrometer
    MeSH term(s) Adult ; Age Factors ; Elasticity/physiology ; Elasticity Imaging Techniques ; Female ; Humans ; Middle Aged ; Skin/diagnostic imaging ; Skin Physiological Phenomena
    Language English
    Publishing date 2018-02-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1229160-2
    ISSN 1600-0846 ; 0909-752X ; 1397-1344
    ISSN (online) 1600-0846
    ISSN 0909-752X ; 1397-1344
    DOI 10.1111/srt.12455
    Database MEDical Literature Analysis and Retrieval System OnLINE

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