LIVIVO - Search results -

Search results

Result 1 - 10 of total 69

Search options

Article ; Online: Antiviral mechanisms of sorafenib against foot-and-mouth disease virus via c-RAF and AKT/PI3K pathways.

Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Semkum, Ploypailin / Lekcharoensuk, Porntippa

2023 Volume 48, Issue 1, Page(s) 329–343

Abstract: Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen that poses a significant threat to the global livestock industry. However, specific antiviral treatments against FMDV are currently unavailable. This study aimed to evaluate the ... ...

Abstract	Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen that poses a significant threat to the global livestock industry. However, specific antiviral treatments against FMDV are currently unavailable. This study aimed to evaluate the antiviral activity of anticancer drugs, including kinase and non-kinase inhibitors against FMDV replication in BHK-21 cells. Sorafenib, a multi-kinase inhibitor, demonstrated a significant dose-dependent reduction in FMDV replication. It exhibited a half maximal effective concentration (EC50) value of 2.46 µM at the pre-viral entry stage and 2.03 µM at the post-viral entry stage. Further intracellular assays revealed that sorafenib effectively decreased 3D
MeSH term(s)	Animals ; Foot-and-Mouth Disease Virus ; Sorafenib/pharmacology ; Sorafenib/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphatidylinositol 3-Kinases/pharmacology ; Proto-Oncogene Proteins c-akt/metabolism ; Proto-Oncogene Proteins c-akt/pharmacology ; Foot-and-Mouth Disease ; Molecular Docking Simulation ; Cell Line ; Antiviral Agents/pharmacology ; Virus Replication
Chemical Substances	Sorafenib (9ZOQ3TZI87) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Antiviral Agents
Language	English
Publishing date	2023-09-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	406735-6
ISSN	1573-7446 ; 0165-7380
ISSN (online)	1573-7446
ISSN	0165-7380
DOI	10.1007/s11259-023-10211-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3453: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article: First study on

Triratapiban, Chanittha / Lueangaramkul, Varanya / Phecharat, Nantawan / Pantanam, Achiraya / Lekcharoensuk, Porntippa / Theerawatanasirikul, Sirin

Veterinary world

2023 Volume 16, Issue 3, Page(s) 618–630

Abstract: Background and aim: Feline infectious peritonitis (FIP), one of the most important infectious diseases in cats is caused by FIP virus (FIPV), a mutated variant of feline coronavirus. Feline infectious peritonitis has a negative impact on feline health, ... ...

Abstract	Background and aim: Feline infectious peritonitis (FIP), one of the most important infectious diseases in cats is caused by FIP virus (FIPV), a mutated variant of feline coronavirus. Feline infectious peritonitis has a negative impact on feline health, with extremely high mortality in clinical FIP-infected cats, particularly young cats. There are no approved drugs for FIP treatment, and therapeutic possibilities for FIP treatment are limited. This study aimed to utilize nature-derived bioactive flavonoids with antiviral properties to inhibit FIPV infection in Crandell-Rees feline kidney (CRFK) cells. Materials and methods: The cytotoxicity of 16 flavonoids was evaluated on CRFK cells using a colorimetric method (MTS) assay. Viral kinetics of FIPV at 50 tissue culture infectious dose (TCID Results: Two flavonoids, namely, isoginkgetin and luteolin, inhibited FIPV replication during post-viral entry in a dose-dependent manner, with 50% maximal effective concentrations = 4.77 ± 0.09 and 36.28 ± 0.03 μM, respectively. Based on viral kinetics, both flavonoids could inhibit FIPV replication at the early stage of infection at 0-6-HPI for isoginkgetin and 2-6-HPI for luteolin using a time-of-addition assay. Isoginkgetin exerted a direct virucidal effect that reduced the viral titers by 2 and 1.89 log Conclusion: Isoginkgetin interfered with FIPV replication during both post-viral infection and virucidal experiments on CRFK cells, whereas luteolin inhibited the virus after infection. These results demonstrate the potential of herbal medicine for treating FIP.
Language	English
Publishing date	2023-03-26
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2023.618-630
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Hydrogel-based 3D human iPSC-derived neuronal culture for the study of rabies virus infection.

Muangsanit, Papon / Chailangkarn, Thanathom / Tanwattana, Nathiphat / Wongwanakul, Ratjika / Lekcharoensuk, Porntippa / Kaewborisuth, Challika

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1215205

Abstract: Background: Rabies is a highly fatal infectious disease that poses a significant threat to human health in developing countries. In vitro study-based understanding of pathogenesis and tropism of different strains of rabies virus (RABV) in the central ... ...

Abstract	Background: Rabies is a highly fatal infectious disease that poses a significant threat to human health in developing countries. In vitro study-based understanding of pathogenesis and tropism of different strains of rabies virus (RABV) in the central nervous system (CNS) is limited due to the lack of suitable culture models that recapitulate the complex communication pathways among host cells, extracellular matrices, and viruses. Therefore, a three-dimensional (3D) cell culture that mimics cell-matrix interactions, resembling in vivo microenvironment, is necessary to discover relevant underlying mechanisms of RABV infection and host responses. Methods: The 3D collagen-Matrigel hydrogel encapsulating hiPSC-derived neurons for RABV infection was developed and characterized based on cell viability, morphology, and gene expression analysis of neuronal markers. The replication kinetics of two different strains of RABV [wild-type Thai (TH) and Challenge Virus Standard (CVS)-11 strains] in both 2D and 3D neuronal cultures were examined. Differential gene expression analysis (DEG) of the neuropathological pathway of RABV-infected 2D and 3D models was also investigated via NanoString analysis. Results: The 3D hiPSC-derived neurons revealed a more physiologically interconnected neuronal network as well as more robust and prolonged maturation and differentiation than the conventional 2D monolayer model. TH and CVS-11 exhibited distinct growth kinetics in 3D neuronal model. Additionally, gene expression analysis of the neuropathological pathway observed during RABV infection demonstrated a vast number of differentially expressed genes (DEGs) in 3D model. Unlike 2D neuronal model, 3D model displayed more pronounced cellular responses upon infection with CVS-11 when compared to the TH-infected group, highlighting the influence of the cell environment on RABV-host interactions. Gene ontology (GO) enrichment of DEGs in the infected 3D neuronal culture showed alterations of genes associated with the inflammatory response, apoptotic signaling pathway, glutamatergic synapse, and trans-synaptic signaling which did not significantly change in 2D culture. Conclusion: We demonstrated the use of a hydrogel-based 3D hiPSC-derived neuronal model, a highly promising technology, to study RABV infection in a more physiological environment, which will broaden our understanding of RABV-host interactions in the CNS.
MeSH term(s)	Humans ; Rabies virus ; Rabies ; Induced Pluripotent Stem Cells ; Hydrogels ; Neurons
Chemical Substances	Hydrogels
Language	English
Publishing date	2023-08-25
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1215205
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: The Application of the Gibson Assembly Method in the Production of Two pKLS3 Vector-Derived Infectious Clones of Foot-and-Mouth Disease Virus.

Semkum, Ploypailin / Thangthamniyom, Nattarat / Chankeeree, Penpitcha / Keawborisuth, Challika / Theerawatanasirikul, Sirin / Lekcharoensuk, Porntippa

Vaccines

2023 Volume 11, Issue 6

Abstract: The construction of a full-length infectious clone, essential for molecular virological study and vaccine development, is quite a challenge for viruses with long genomes or possessing complex nucleotide sequence structures. Herein, we have constructed ... ...

Abstract	The construction of a full-length infectious clone, essential for molecular virological study and vaccine development, is quite a challenge for viruses with long genomes or possessing complex nucleotide sequence structures. Herein, we have constructed infectious clones of foot-and-mouth disease virus (FMDV) types O and A by joining each viral coding region with our pKLS3 vector in a single isothermal reaction using Gibson Assembly (GA). pKLS3 is a 4.3-kb FMDV minigenome. To achieve optimal conditions for the DNA joining, each FMDV coding sequence was divided into two overlapping fragments of approximately 3.8 and 3.2 kb, respectively. Both DNA fragments contain the introduced linker sequences for assembly with the linearized pKLS3 vector. FMDV infectious clones were produced upon directly transfecting the GA reaction into baby hamster kidney-21 (BHK-21) cells. After passing in BHK-21 cells, both rescued FMDVs (rO189 and rNP05) demonstrated growth kinetics and antigenicity similar to their parental viruses. Thus far, this is the first report on GA-derived, full-length infectious FMDV cDNA clones. This simple DNA assembly method and the FMDV minigenome would facilitate the construction of FMDV infectious clones and enable genetic manipulation for FMDV research and custom-made FMDV vaccine production.
Language	English
Publishing date	2023-06-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines11061111
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Small Molecules Targeting 3C Protease Inhibit FMDV Replication and Exhibit Virucidal Effect in Cell-Based Assays.

Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Pantanam, Achiraya / Mana, Natjira / Semkum, Ploypailin / Lekcharoensuk, Porntippa

Viruses

2023 Volume 15, Issue 9

Abstract: Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals, caused by the foot-and-mouth disease virus (FMDV). It is endemic in Asia and Africa but spreads sporadically throughout the world, resulting in significant losses in ... ...

Abstract	Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals, caused by the foot-and-mouth disease virus (FMDV). It is endemic in Asia and Africa but spreads sporadically throughout the world, resulting in significant losses in the livestock industry. Effective anti-FMDV therapeutics could be a supportive control strategy. Herein, we utilized computer-aided, structure-based virtual screening to filter lead compounds from the National Cancer Institute (NCI) diversity and mechanical libraries using FMDV 3C protease (3C
MeSH term(s)	Animals ; Peptide Hydrolases ; Foot-and-Mouth Disease Virus ; Molecular Docking Simulation ; Endopeptidases ; Antiviral Agents/pharmacology ; 3C Viral Proteases
Chemical Substances	Peptide Hydrolases (EC 3.4.-) ; Endopeptidases (EC 3.4.-) ; Antiviral Agents
Language	English
Publishing date	2023-09-06
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15091887
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Characterization and complete genome analysis of a novel Escherichia phage, vB_EcoM-RPN242.

Imklin, Napakhwan / Sriprasong, Pattaraporn / Thanantong, Narut / Lekcharoensuk, Porntippa / Nasanit, Rujikan

Archives of virology

2022 Volume 167, Issue 8, Page(s) 1675–1679

Abstract: The novel Escherichia phage vB_EcoM-RPN242 was isolated using a strain of Escherichia coli originating from a diarrheic piglet as a host. The phage was able to form plaques on the E. coli lawn at 15-45 °C. Moreover, it was stable over a wide pH (4-10) ... ...

Abstract	The novel Escherichia phage vB_EcoM-RPN242 was isolated using a strain of Escherichia coli originating from a diarrheic piglet as a host. The phage was able to form plaques on the E. coli lawn at 15-45 °C. Moreover, it was stable over a wide pH (4-10) and temperature (4-70 °C) range. The vB_EcoM-RPN242 genome was found to be a linear, double-stranded DNA consisting of 154,840 base pairs. There were 195 protein-encoding genes and two tRNAs detected in the genome; however, no genes associated with virulence, toxins or antimicrobial resistance were found. According to overall nucleotide sequence comparisons, vB_EcoM-RPN242 possibly represents a new species in the genus Agtrevirus.
MeSH term(s)	Animals ; Bacteriophages/genetics ; Escherichia coli/genetics ; Genome, Viral ; High-Throughput Nucleotide Sequencing ; Sequence Analysis, DNA ; Swine
Language	English
Publishing date	2022-05-22
Publishing country	Austria
Document type	Journal Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-022-05479-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Ud II Zs.110: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Andrographolide and Deoxyandrographolide Inhibit Protease and IFN-Antagonist Activities of Foot-and-Mouth Disease Virus 3Cpro

Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Thangthamniyom, Nattarat / Chankeeree, Penpitcha / Semkum, Ploypailin / Lekcharoensuk, Porntippa

Animals. 2022 Aug. 07, v. 12, no. 15

2022

Abstract: Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment ... ...

Abstract	Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment available. Several studies have shown that plant-derived products with antiviral properties were effective on viral diseases. Herein, antiviral activities of andrographolide (AGL), deoxyandrographolide (DAG), and neoandrographolide (NEO) against FMDV serotype A were investigated using an in vitro cell-based assay. The results showed that AGL and DAG inhibited FMDV in BHK-21 cells. The inhibitory effects of AGL and DAG were evaluated by RT-qPCR and exhibited EC50 values of 52.18 ± 0.01 µM (SI = 2.23) and 36.47 ± 0.07 µM (SI = 9.22), respectively. The intracellular protease assay revealed that AGL and DAG inhibited FMDV 3Cᵖʳᵒ with IC50 of 67.43 ± 0.81 and 25.58 ± 1.41 µM, respectively. Additionally, AGL and DAG significantly interfered with interferon (IFN) antagonist activity of the 3Cᵖʳᵒ by derepressing interferon-stimulating gene (ISGs) expression. The molecular docking confirmed that the andrographolides preferentially interacted with the 3Cᵖʳᵒ active site. However, NEO had no antiviral effect in any of the assays. Conclusively, AGL and DAG inhibited FMDV serotype A by interacting with the 3Cᵖʳᵒ and hindered its protease and IFN antagonist activities.
Keywords	Foot-and-mouth disease virus ; active sites ; andrographolide ; antagonists ; antiviral properties ; genes ; inhibitory concentration 50 ; interferons ; livestock ; median effective concentration ; proteinases ; serotypes ; vaccination
Language	English
Dates of publication	2022-0807
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12151995
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Characterization and complete genome analysis of a novel Escherichia phage, vB_EcoM-RPN242

Imklin, Napakhwan / Sriprasong, Pattaraporn / Thanantong, Narut / Lekcharoensuk, Porntippa / Nasanit, Rujikan

Archives of virology. 2022 Aug., v. 167, no. 8

2022

Abstract: The novel Escherichia phage vB_EcoM-RPN242 was isolated using a strain of Escherichia coli originating from a diarrheic piglet as a host. The phage was able to form plaques on the E. coli lawn at 15–45 °C. Moreover, it was stable over a wide pH (4–10) ... ...

Abstract	The novel Escherichia phage vB_EcoM-RPN242 was isolated using a strain of Escherichia coli originating from a diarrheic piglet as a host. The phage was able to form plaques on the E. coli lawn at 15–45 °C. Moreover, it was stable over a wide pH (4–10) and temperature (4–70 °C) range. The vB_EcoM-RPN242 genome was found to be a linear, double-stranded DNA consisting of 154,840 base pairs. There were 195 protein-encoding genes and two tRNAs detected in the genome; however, no genes associated with virulence, toxins or antimicrobial resistance were found. According to overall nucleotide sequence comparisons, vB_EcoM-RPN242 possibly represents a new species in the genus Agtrevirus.
Keywords	DNA ; Escherichia coli ; antibiotic resistance ; bacteriophages ; lawns and turf ; new species ; nucleotide sequences ; pH ; piglets ; sequence analysis ; temperature ; virology ; virulence
Language	English
Dates of publication	2022-08
Size	p. 1675-1679.
Publishing place	Springer Vienna
Document type	Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-022-05479-7
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 70/128: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Non-Nucleoside Inhibitors Decrease Foot-and-Mouth Disease Virus Replication by Blocking the Viral 3D

Theerawatanasirikul, Sirin / Semkum, Ploypailin / Lueangaramkul, Varanya / Chankeeree, Penpitcha / Thangthamniyom, Nattarat / Lekcharoensuk, Porntippa

Viruses

2022 Volume 15, Issue 1

Abstract: Foot-and-mouth disease virus (FMDV), an economically important pathogen of cloven-hoofed livestock, is a positive-sense, single-stranded RNA virus classified in ... ...

Abstract	Foot-and-mouth disease virus (FMDV), an economically important pathogen of cloven-hoofed livestock, is a positive-sense, single-stranded RNA virus classified in the
MeSH term(s)	Animals ; Foot-and-Mouth Disease Virus/genetics ; Antiviral Agents/pharmacology ; Antiviral Agents/metabolism ; RNA-Dependent RNA Polymerase/metabolism ; Foot-and-Mouth Disease ; Virus Replication
Chemical Substances	Antiviral Agents ; RNA-Dependent RNA Polymerase (EC 2.7.7.48)
Language	English
Publishing date	2022-12-30
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15010124
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Andrographolide and Deoxyandrographolide Inhibit Protease and IFN-Antagonist Activities of Foot-and-Mouth Disease Virus 3C

Theerawatanasirikul, Sirin / Lueangaramkul, Varanya / Thangthamniyom, Nattarat / Chankeeree, Penpitcha / Semkum, Ploypailin / Lekcharoensuk, Porntippa

Animals : an open access journal from MDPI

2022 Volume 12, Issue 15

Abstract	Foot-and mouth-disease (FMD) caused by the FMD virus (FMDV) is highly contagious and negatively affects livestock worldwide. The control of the disease requires a combination of measures, including vaccination; however, there is no specific treatment available. Several studies have shown that plant-derived products with antiviral properties were effective on viral diseases. Herein, antiviral activities of andrographolide (AGL), deoxyandrographolide (DAG), and neoandrographolide (NEO) against FMDV serotype A were investigated using an in vitro cell-based assay. The results showed that AGL and DAG inhibited FMDV in BHK-21 cells. The inhibitory effects of AGL and DAG were evaluated by RT-qPCR and exhibited EC50 values of 52.18 ± 0.01 µM (SI = 2.23) and 36.47 ± 0.07 µM (SI = 9.22), respectively. The intracellular protease assay revealed that AGL and DAG inhibited FMDV 3C
Language	English
Publishing date	2022-08-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani12151995
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito