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Article ; Online: Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production

Fan Yang / Lina Chen / Bolun Wen / Xiaodi Wang / Lele Wang / Kaiyuan Ji / Huishu Liu

International Journal of Molecular Sciences, Vol 24, Iss 10116, p

2023 Volume 10116

Abstract: The mechanism of maintaining myometrial contractions during labor remains unclear. Autophagy has been reported to be activated in laboring myometrium, along with the high expression of Golgi reassembly stacking protein 2 (GORASP2), a protein capable of ... ...

Abstract	The mechanism of maintaining myometrial contractions during labor remains unclear. Autophagy has been reported to be activated in laboring myometrium, along with the high expression of Golgi reassembly stacking protein 2 (GORASP2), a protein capable of regulating autophagy activation. This study aimed to investigate the role and mechanism of GORASP2 in uterine contractions during labor. Western blot confirmed the increased expression of GORASP2 in laboring myometrium. Furthermore, the knockdown of GORASP2 in primary human myometrial smooth muscle cells (hMSMCs) using siRNA resulted in reduced cell contractility. This phenomenon was independent of the contraction-associated protein and autophagy. Differential mRNAs were analyzed using RNA sequencing. Subsequently, KEGG pathway analysis identified that GORASP2 knockdown suppressed several energy metabolism pathways. Furthermore, reduced ATP levels and aerobic respiration impairment were observed in measuring the oxygen consumption rate (OCR). These findings suggest that GORASP2 is up-regulated in the myometrium during labor and modulates myometrial contractility mainly by maintaining ATP production.
Keywords	labor ; myometrial contractility ; GORASP2 ; ATP production ; pregnancy ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Degradation of emerald green

Zhimin Li / Lele Wang / Huili Chen / Qinglin Ma

Heritage Science, Vol 8, Iss 1, Pp 1-

scientific studies on multi-polychrome Vairocana Statue in Dazu Rock Carvings, Chongqing, China

2020 Volume 12

Abstract: Abstract The spontaneous degradation of pigments in painting may occur depending on environment, especially the surrounding condition of high humidity and soluble salts. Even some of these are well studied, more investigations are still in need for ... ...

Abstract	Abstract The spontaneous degradation of pigments in painting may occur depending on environment, especially the surrounding condition of high humidity and soluble salts. Even some of these are well studied, more investigations are still in need for heritage science. Paint cross sections from Vairocana Statue in Dazu Rock Carvings, one of World Cultural Heritage, Chongqing, China are studied by optical microscopy (OM), Raman spectroscopy (Raman) and mapping, and scanning electron microscopy coupled with energy dispersive X-ray analysis (SEM–EDS). Ultramarine blue, emerald green, synthetic atacamite, vermilion, red lead, gold foil, orpiment, cerussite, gypsum and barite are identified as pigments. Moreover, lavendulan (NaCaCu5(AsO4)4Cl·5H2O) and mimetite (Pb5(AsO4)3Cl) are degradation products of emerald green (Cu(C2H3O2)2·3Cu(AsO2)2) and cerussite(PbCO3) originally used as green pigment with mixture. These secondary products are formed via multistep progresses as degradation of emerald green and cerussite, oxidation of arsenite ((AsO2)−), migration of arsenate ((AsO4)3−) and precipitation reaction of these ions with other necessary ions from environmental conditions. Based on the pigment and paint layer stratigraphy analysis, the paintings of the statue have been executed at least four times and the most recent may be after 1850s. The formation of lavendulan give rise to a highlight for identification of green copper-arsenic containing pigments and characterization of degradation in complex conditions.
Keywords	Dazu Rock Carvings ; Pigment ; Degradation ; Raman mapping ; Emerald green ; Lavendulan ; Fine Arts ; N ; Analytical chemistry ; QD71-142
Subject code	669
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	SpringerOpen
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Influence of dietary

Kamal, Mahmoud / Lele, Wang / Shuzhen, Tang / Jiandi, Liang / Rongyan, Qin / Yanfeng, Liu / Wenqi, Wang / Xiangyu, Chen / Cheng, Yanfen

Frontiers in microbiology

2024 Volume 15, Page(s) 1377314

Abstract: This experiment aimed to examine the impact ... ...

Abstract	This experiment aimed to examine the impact of
Language	English
Publishing date	2024-04-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2024.1377314
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Construction of UiO-66/Bi4O5Br2 Type-II Heterojunction to Boost Charge Transfer for Promoting Photocatalytic CO2 Reduction Performance

Dongsheng Li / Bichen Zhu / Zhongti Sun / Qinqin Liu / Lele Wang / Hua Tang

Frontiers in Chemistry, Vol

2021 Volume 9

Abstract: One of the basic challenges of CO2 photoreduction is to develop efficient photocatalysts, and the construction of heterostructure photocatalysts with intimate interfaces is an effective strategy to enhance interfacial charge transfer for realizing high ... ...

Abstract	One of the basic challenges of CO2 photoreduction is to develop efficient photocatalysts, and the construction of heterostructure photocatalysts with intimate interfaces is an effective strategy to enhance interfacial charge transfer for realizing high photocatalytic activity. Herein, a novel UiO-66/Bi4O5Br2 heterostructure photocatalyst was constructed by depositing UiO-66 nanoparticles with octahedral morphology over the Bi4O5Br2 nanoflowers assembled from the Bi4O5Br2 nanosheets via an electrostatic self-assembly method. A tight contact interface and a built-in electric field were formed between the UiO-66 and the Bi4O5Br2, which was conducive to the photo-electrons transfer from the Bi4O5Br2 to the UiO-66 and the formation of a type-II heterojunction with highly efficient charge separation. As a result, the UiO-66/Bi4O5Br2 exhibited improved photocatalytic CO2 reduction performance with a CO generation rate of 8.35 μmol h−1 g−1 without using any sacrificial agents or noble co-catalysts. This work illustrates an applicable tactic to develop potent photocatalysts for clean energy conversion.
Keywords	UiO-66 ; Bi4O5Br2 ; photocatalytic ; CO2 reduction ; type-II heterojunction ; Chemistry ; QD1-999
Subject code	500
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A new Eimeria coccidian species (Apicomplexa

Weimin Cai / Zeyang Suding / Lele Wang / Zhaofeng Hou / Dandan Liu / Siyang Huang / Jinjun Xu / Jianping Tao

BMC Veterinary Research, Vol 18, Iss 1, Pp 1-

Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China

2022 Volume 12

Abstract: Abstract Background Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world’s endangered ...

Abstract	Abstract Background Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world’s endangered species of Père David’s deer (Elaphurus davidianus). Therefore, the purpose of this study is to identify an unknown Eimeria genus in the Père David’s deer in Dafeng Milu National Nature Reserve, China. Results A new Eimeria species is described from Père David’s deer. Sporulated oocysts (n = 54) are pyriform, with a rough, yellowish brown, 2-layered oocyst wall (2.5 μm thick). A numerous small granules are dispersed randomly on the wall. Oocysts measured 41.2 (39.2–42.8) μm × 29.5 (27.9–30.5) μm, oocyst length/width (L/W) ratio, 1.4. Oocyst residuum, a polar granule and a polar cap are absent. The micropyle (3.5 μm wide) is present. Sporocysts are spindle shaped, 18.2 (16.5–20.0) μm × 10.5 (9.8–11.9) μm, sporocyst L/W ratio, 1.7 (1.5–1.9). A thin convex Stieda body is present and the sporocyst residuum is composed of numerous small granules less than 2.0 μm in diameter dispersed randomly. Each sporocyst contained 2 comma-shaped sporozoites in head-to-tail arrangement. A nucleus is located immediately anterior to the posterior, strong refractive and subspherical refractile body (~ 8 μm). Molecular analysis was conducted at the 18S, ITS-1 and COI loci. Conclusion Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria davidianusi after its host, the Père David’s deer (Elaphurus davidianus).
Keywords	Père David’s deer ; Morphology ; Eimeria davidianusi ; Phylogenetics ; Veterinary medicine ; SF600-1100
Subject code	590
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Environmentally-friendly strategy for separation of α-lactalbumin from whey by aqueous two phase flotation

Bin Jiang / Lele Wang / Jiaxin Na / Xiaoqing Zhang / Yongqiang Yuan / Chunhong Liu / Zhibiao Feng

Arabian Journal of Chemistry, Vol 13, Iss 1, Pp 3391-

2020 Volume 3402

Abstract: Aqueous two-phase flotation (ATPF) consisting of 1000 g/mol polyethylene glycol (PEG 1000)/trisodium citrate was developed for the separation of α-Lactalbumin (α-La) from whey. The flotation efficiency (E) and purification factor (PF) of α-La were ... ...

Abstract	Aqueous two-phase flotation (ATPF) consisting of 1000 g/mol polyethylene glycol (PEG 1000)/trisodium citrate was developed for the separation of α-Lactalbumin (α-La) from whey. The flotation efficiency (E) and purification factor (PF) of α-La were evaluated using the reversed-phase high-performance liquid chromatography (RP-HPLC). The effects of pH, concentration of trisodium citrate, flow velocity, flotation time and whey loading on the E and PF of α-La were investigated. An efficient separation of α-La from whey was achieved using ATPF with pH of 8.20, 5 mL of 0.50 g/mL PEG 1000 solution, 35 mL of 0.40 g/mL trisodium citrate solution and whey (20%, v/v), 30 mL/min of flow velocity and 42 min of flotation time. Under the optimal conditions, E and PF of α-La could reach 87.54% and 5.33, respectively. In addition, the kinetic process of the separation of α-La by ATPF of PEG 1000/trisodium citrate was investigated. The results showed that there were two stages in the separation process. Both stages obeyed the first-order kinetic equation, and the first stage was faster than the second one. Keywords: ATPF, Whey, α-Lactalbumin, Flotation efficiency, RP-HPLC, Kinetic
Keywords	Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Eco-Innovation in Reusing Food By-Products

Bin Jiang / Jiaxin Na / Lele Wang / Dongmei Li / Chunhong Liu / Zhibiao Feng

Polymers, Vol 11, Iss 2, p

Separation of Ovalbumin from Salted Egg White Using Aqueous Two-Phase System of PEG 1000/(NH 4 ) 2 SO 4

2019 Volume 238

Abstract: For the purpose of reducing pollution and the rational use of salted egg white, which is a byproduct of the manufacturing process of salted egg yolk, an aqueous two-phase system (ATPS) composed of polyethylene glycols (PEG 1000) and (NH 4 ) 2 SO 4 was ... ...

Abstract	For the purpose of reducing pollution and the rational use of salted egg white, which is a byproduct of the manufacturing process of salted egg yolk, an aqueous two-phase system (ATPS) composed of polyethylene glycols (PEG 1000) and (NH 4 ) 2 SO 4 was investigated to selectively separate ovalbumin (OVA) from salted egg white. With the aim of optimizing the selective separation of OVA using ATPS, a response surface method (RSM) experiment was carried out on the basis of a single-factor experiment. The OVA was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS⁻PAGE), reversed-phase high-performance liquid chromatography (RP-HPLC), liquid chromatography-nano electrospray ionization mass spectrometry (Nano LC-ESI-MS/MS), and Fourier transform infrared spectroscopy (FT-IR). Under the optimal conditions, the recovery yield of OVA through ATPS ( Y ) and the purity of OVA ( P ) could reach 89.25% and 96.28%, respectively. In conclusion, OVA was successfully separated from the salted egg white by PEG/(NH 4 ) 2 SO 4 ATPS.
Keywords	polyethylene glycols ; aqueous two-phase system ; salted egg white ; ovalbumin ; liquid chromatography-nano electrospray ionization mass spectrometry ; Organic chemistry ; QD241-441
Subject code	290
Language	English
Publishing date	2019-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Bacterial community analysis of anoxic/aeration (A/O) system in a combined process for gibberellin wastewater treatment.

Erming Ouyang / Yao Lu / Jiating Ouyang / Lele Wang / Xiaohui Wang

PLoS ONE, Vol 12, Iss 10, p e

2017 Volume 0186743

Abstract: Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological ... ...

Abstract	Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological contact oxidation combined processes are used to treat gibberellin wastewater. However, knowledge of the treatment effects of the A/O process and bacterial community structure in the aeration tank reactors of such systems is sparse. Therefore, this study was conducted to investigate the treatment effects and operation of the A/O process on gibberellin wastewater, as well as changes in the bacterial community structure of activated sludge in the aeration tank during treatment. Moreover, removal was examined based on evaluation of effluent after A/O treatment. Although influent chemical oxygen demand (COD), NH3-N and total phosphorus (TP) fluctuated, effluent COD, NH3-N and TP remained stable. Moreover, average COD, NH3-N and TP removal efficiency were 68.41%, 93.67% and 45.82%, respectively, during the A/O process. At the phylum level, Proteobacteria was the dominant phylum in all samples, followed by Chloroflexi, Bacteroidetes and Actinobacteria. Proteobacteria played an important role in the removal of organic matter. Chloroflexi was found to be responsible for the degradation of carbohydrates and Bacteroidetes also had been found to be responsible for the degradation of complex organic matters. Actinobacteria are able to degrade a variety of environmental chemicals. Additionally, Anaerolineaceae_uncultured was the major genus in samples collected on May 25, 2015, while Novosphingobium and Nitrospira were dominant in most samples. Nitrosomonas are regarded as the dominant ammonia-oxidizing bacteria, while Nitrospira are the main nitrite-oxidizing bacteria. Bacterial community structure varied considerably with time, and a partial Mantel test showed a highly significant positive correlation between bacterial community structure and DO. The bacterial community ...
Keywords	Medicine ; R ; Science ; Q
Subject code	660
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: All-fiber high-speed image detection enabled by deep learning

Zhoutian Liu / Lele Wang / Yuan Meng / Tiantian He / Sifeng He / Yousi Yang / Liuyue Wang / Jiading Tian / Dan Li / Ping Yan / Mali Gong / Qiang Liu / Qirong Xiao

Nature Communications, Vol 13, Iss 1, Pp 1-

2022 Volume 8

Abstract: Here, the authors demonstrate high-speed imaging through multimode optical fibers by using the high intermodal dispersion to transform 2D spatial information into 1D temporal pulsed signal streams. Deep learning is used to reconstruct the images of ... ...

Abstract	Here, the authors demonstrate high-speed imaging through multimode optical fibers by using the high intermodal dispersion to transform 2D spatial information into 1D temporal pulsed signal streams. Deep learning is used to reconstruct the images of micron-scale objects at high frame rates.
Keywords	Science ; Q
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Estimating the time of last drinking from blood ethyl glucuronide and ethyl sulphate concentrations

Lele Wang / Wei Zhang / Ruilong Wang / Yongli Guang / Daming Zhang / Chao Zhang / Meng Hu / Zhiwen Wei / Wenfang Zhang / Keming Yun / Zhongyuan Guo

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 7

Abstract: Abstract The determination of length of time from the last drinking is critical for cases like drunk driving, sexual assault victims, and also postmortem suspected poisoning cases. The study was aimed to established a method of estimating the time of ... ...

Abstract	Abstract The determination of length of time from the last drinking is critical for cases like drunk driving, sexual assault victims, and also postmortem suspected poisoning cases. The study was aimed to established a method of estimating the time of last drinking through the pharmacokinetic study of conjugation metabolites of alcohol in blood after a single oral dose. Twenty-six volunteers (14 males) consumed alcohol with food at a fixed dose of 0.72 g/kg after fasting for 12 h. Five milliliters of blood were collected 120 h after the start of drinking, and all samples were analyzed with headspace-gas chromatography and high-performance liquid chromatography–tandem mass spectrometry. The time point of last drinking was estimated through the relationship between the concentration ratio of ethyl glucuronide to ethyl sulphate and the length of time after drinking. Pharmacokinetic parameters were analyzed by a pharmacokinetic software DAS according to the non-compartment model. A good correlation model was obtained from the relationship between concentration ratio of ethyl glucuronide to ethyl sulphate in blood and the time of alcohol use, and the margin of error was mostly lower than 10%. The time of maximum concentration, maximum concentration, and elimination half-life of ethyl glucuronide in blood were 4.12 ± 1.07 h, 0.31 ± 0.11 mg/L and 2.56 ± 0.89 h; the time of maximum concentration, maximum concentration, and elimination half-life of ethyl sulphate in blood were 3.02 ± 0.70 h, 0.17 ± 0.04 mg/L, and 2.04 ± 0.76 h. The study established a potential method to estimate the length of time after a moderate oral dose, and provided pharmacokinetic parameters of ethyl glucuronide and ethyl sulphate in Chinese population.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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