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Article ; Online: Vivre avec une hypercholestérolémie familiale.

Lemaître, Véronique

2020 Volume 69, Issue 9, Page(s) 999–1000

Title translation	Living with familial hypercholesterolemia.
MeSH term(s)	Humans ; Hyperlipoproteinemia Type II
Language	French
Publishing date	2020-04-01
Publishing country	France
Document type	Journal Article
ZDB-ID	205365-2
ISSN	2101-017X ; 0035-2640
ISSN (online)	2101-017X
ISSN	0035-2640
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Article ; Online: Surgical Management of a Salter-Harris Type I Distal Physeal Fracture of the Tibia in a Calf: A Case Report.

Lemaitre, Victor / Cote, Emeline / Bellon, Christophe / Cassard, Hervé / Schelcher, François / Maillard, Renaud / Robcis, Rodolphe

Veterinary sciences

2023 Volume 10, Issue 7

Abstract: Fractures are common conditions in cattle, including tibial fractures. Physeal tibial fractures are more specific and less frequently met in field conditions. A calf with a Salter-Harris type I distal physeal fracture of the tibia was referred to the ... ...

Abstract	Fractures are common conditions in cattle, including tibial fractures. Physeal tibial fractures are more specific and less frequently met in field conditions. A calf with a Salter-Harris type I distal physeal fracture of the tibia was referred to the National Veterinary School of Toulouse (ENVT), France. Although the use of external fixators in the treatment of tibial fractures is common, distal physeal tibial fractures require a different and specific technique involving them. They were first used as a lever arm to reduce the fracture due to the severe displacement. A hock joint bypass was then performed. Six weeks after treatment, the calf recovered successfully from the use of the affected limb without any adverse sequelae. The present case provides management of a distal tibial fracture using external fixators. This innovative and accessible surgical technique may be used by veterinary practitioners in future similar cases of distal tibial fractures when pins in the distal end cannot be inserted.
Language	English
Publishing date	2023-07-14
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci10070463
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Article ; Online: Deep Learning-Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard-to-Transfect Cells.

Mukherjee, Prithvijit / Patino, Cesar A / Pathak, Nibir / Lemaitre, Vincent / Espinosa, Horacio D

Small (Weinheim an der Bergstrasse, Germany)

2022 Volume 18, Issue 20, Page(s) e2107795

Abstract: Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene editing ... ...

Abstract	Genome engineering of cells using CRISPR/Cas systems has opened new avenues for pharmacological screening and investigating the molecular mechanisms of disease. A critical step in many such studies is the intracellular delivery of the gene editing machinery and the subsequent manipulation of cells. However, these workflows often involve processes such as bulk electroporation for intracellular delivery and fluorescence activated cell sorting for cell isolation that can be harsh to sensitive cell types such as human-induced pluripotent stem cells (hiPSCs). This often leads to poor viability and low overall efficacy, requiring the use of large starting samples. In this work, a fully automated version of the nanofountain probe electroporation (NFP-E) system, a nanopipette-based single-cell electroporation method is presented that provides superior cell viability and efficiency compared to traditional methods. The automated system utilizes a deep convolutional network to identify cell locations and a cell-nanopipette contact algorithm to position the nanopipette over each cell for the application of electroporation pulses. The automated NFP-E is combined with microconfinement arrays for cell isolation to demonstrate a workflow that can be used for CRISPR/Cas9 gene editing and cell tracking with potential applications in screening studies and isogenic cell line generation.
MeSH term(s)	CRISPR-Cas Systems/genetics ; Deep Learning ; Electroporation/methods ; Gene Editing/methods ; Humans ; Induced Pluripotent Stem Cells/metabolism
Language	English
Publishing date	2022-03-21
Publishing country	Germany
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ZDB-ID	2168935-0
ISSN	1613-6829 ; 1613-6810
ISSN (online)	1613-6829
ISSN	1613-6810
DOI	10.1002/smll.202107795
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Article: A fine-tuned defense at the pea root caps: Involvement of border cells and arabinogalactan proteins against soilborne diseases.

Fortier, Mélanie / Lemaitre, Vincent / Gaudry, Alexia / Pawlak, Barbara / Driouich, Azeddine / Follet-Gueye, Marie-Laure / Vicré, Maïté

Frontiers in plant science

2023 Volume 14, Page(s) 1132132

Abstract: Plants have to cope with a myriad of soilborne pathogens that affect crop production and food security. The complex interactions between the root system and microorganisms are determinant for the whole plant health. However, the knowledge regarding root ... ...

Abstract	Plants have to cope with a myriad of soilborne pathogens that affect crop production and food security. The complex interactions between the root system and microorganisms are determinant for the whole plant health. However, the knowledge regarding root defense responses is limited as compared to the aerial parts of the plant. Immune responses in roots appear to be tissue-specific suggesting a compartmentalization of defense mechanisms in these organs. The root cap releases cells termed root "associated cap-derived cells" (AC-DCs) or "border cells" embedded in a thick mucilage layer forming the root extracellular trap (RET) dedicated to root protection against soilborne pathogens. Pea (
Language	English
Publishing date	2023-02-09
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2023.1132132
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Article ; Online: Deep Learning and Computer Vision Strategies for Automated Gene Editing with a Single-Cell Electroporation Platform.

Patino, Cesar A / Mukherjee, Prithvijit / Lemaitre, Vincent / Pathak, Nibir / Espinosa, Horacio D

SLAS technology

2021 Volume 26, Issue 1, Page(s) 26–36

Abstract: Single-cell delivery platforms like microinjection and nanoprobe electroporation enable unparalleled control over cell manipulation tasks but are generally limited in throughput. Here, we present an automated single-cell electroporation system capable of ...

Abstract	Single-cell delivery platforms like microinjection and nanoprobe electroporation enable unparalleled control over cell manipulation tasks but are generally limited in throughput. Here, we present an automated single-cell electroporation system capable of automatically detecting cells with artificial intelligence (AI) software and delivering exogenous cargoes of different sizes with uniform dosage. We implemented a fully convolutional network (FCN) architecture to precisely locate the nuclei and cytosol of six cell types with various shapes and sizes, using phase contrast microscopy. Nuclear staining or reporter fluorescence was used along with phase contrast images of cells within the same field of view to facilitate the manual annotation process. Furthermore, we leveraged the near-human inference capabilities of the FCN network in detecting stained nuclei to automatically generate ground-truth labels of thousands of cells within seconds, and observed no statistically significant difference in performance compared to training with manual annotations. The average detection sensitivity and precision of the FCN network were 95±1.7% and 90±1.8%, respectively, outperforming a traditional image-processing algorithm (72±7.2% and 72±5.5%) used for comparison. To test the platform, we delivered fluorescent-labeled proteins into adhered cells and measured a delivery efficiency of 90%. As a demonstration, we used the automated single-cell electroporation platform to deliver Cas9-guide RNA (gRNA) complexes into an induced pluripotent stem cell (iPSC) line to knock out a green fluorescent protein-encoding gene in a population of ~200 cells. The results demonstrate that automated single-cell delivery is a useful cell manipulation tool for applications that demand throughput, control, and precision.
MeSH term(s)	Artificial Intelligence ; Computers ; Deep Learning ; Electroporation ; Gene Editing ; Humans
Language	English
Publishing date	2021-01-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2900310-6
ISSN	2472-6311 ; 2472-6303
ISSN (online)	2472-6311
ISSN	2472-6303
DOI	10.1177/2472630320982320
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Article ; Online: Early and comprehensive care bundle in the elderly for acute heart failure in the emergency department: study protocol of the ELISABETH stepped-wedge cluster randomized trial.

Freund, Yonathan / Gorlicki, Judith / Cachanado, Marine / Salhi, Sarah / Lemaître, Vanessa / Simon, Tabassome / Mebazaa, Alexandre

Trials

2019 Volume 20, Issue 1, Page(s) 95

Abstract: Background: Acute heart failure (AHF) is one of the most common diagnoses for elderly patients in the emergency department (ED), with an admission rate above 80% and 1-month mortality around 10%. The European guidelines for the management of AHF are ... ...

Abstract	Background: Acute heart failure (AHF) is one of the most common diagnoses for elderly patients in the emergency department (ED), with an admission rate above 80% and 1-month mortality around 10%. The European guidelines for the management of AHF are based on moderate levels of evidence, due to the lack of randomized controlled trials and the scarce evidence of any clinical added value of a specific treatment to improve outcomes. Recent reports suggest that the very early administration of full recommended therapy may decrease mortality. However, several studies have highlighted that elderly patients often received suboptimal treatment. Our hypothesis is that an early care bundle that comprises early and comprehensive management of symptoms, along with prompt detection and treatment of precipitating factors should improve AHF outcome in elderly patients. Methods/design: ELISABETH is a stepped-wedge, cluster randomized controlled, clinical trial in 15 emergency departments in France recruiting all patients aged 75 years and older with a diagnosis of AHF. The tested intervention is a care bundle with a checklist that mandates detection and early treatment of AHF precipitating factors, early and intensive treatment of congestion with intravenously administered nitrate boluses, and application of other recommended treatment (low-dose diuretics, non-invasive ventilation when indicated, and preventive low-molecular-weight heparin). Each center is randomized to the order in which they will switch from a "control period" to an "intervention period." All centers begin the trials with the control period for 2 weeks, then after each 2-week step a new center will enter the intervention period. At the end of the trial, all clusters will receive the intervention regimen. The primary outcome is the number of days alive and out of the hospital at 30 days. Discussion: If our hypothesis is confirmed, this trial will strengthen the level of evidence of AHF guidelines and stress the importance of the associated early and comprehensive treatment of precipitating factors. This trial could be the first to report a reduction in short-term morbidity and mortality in elderly AHF patients. Trial registration: ClinicalTrials.gov, ID: NCT03683212. Prospectively registered on 25 September 2018.
MeSH term(s)	Acute Disease ; Age Factors ; Aged ; Aged, 80 and over ; Cardiology Service, Hospital/organization & administration ; Checklist ; Delivery of Health Care, Integrated/organization & administration ; Emergency Service, Hospital/organization & administration ; Female ; France ; Heart Failure/diagnosis ; Heart Failure/mortality ; Heart Failure/physiopathology ; Heart Failure/therapy ; Humans ; Male ; Multicenter Studies as Topic ; Patient Care Bundles ; Randomized Controlled Trials as Topic ; Time Factors ; Treatment Outcome
Language	English
Publishing date	2019-01-31
Publishing country	England
Document type	Clinical Trial Protocol ; Journal Article
ZDB-ID	2040523-6
ISSN	1745-6215 ; 1468-6694 ; 1745-6215
ISSN (online)	1745-6215
ISSN	1468-6694 ; 1745-6215
DOI	10.1186/s13063-019-3188-8
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Article ; Online: Monoclonal Cell Line Generation and CRISPR/Cas9 Manipulation via Single-Cell Electroporation.

Yang, Ruiguo / Lemaître, Vincent / Huang, Changjin / Haddadi, Abbas / McNaughton, Rebecca / Espinosa, Horacio D

Small (Weinheim an der Bergstrasse, Germany)

2018 Volume 14, Issue 12, Page(s) e1702495

Abstract: Stably transfected cell lines are widely used in drug discovery and biological research to produce recombinant proteins. Generation of these cell lines requires the isolation of multiple clones, using time-consuming dilution methods, to evaluate the ... ...

Abstract	Stably transfected cell lines are widely used in drug discovery and biological research to produce recombinant proteins. Generation of these cell lines requires the isolation of multiple clones, using time-consuming dilution methods, to evaluate the expression levels of the gene of interest. A new and efficient method is described for the generation of monoclonal cell lines, without the need for dilution cloning. In this new method, arrays of patterned cell colonies and single cell transfection are employed to deliver a plasmid coding for a reporter gene and conferring resistance to an antibiotic. Using a nanofountain probe electroporation system, probe positioning is achieved through a micromanipulator with sub-micron resolution and resistance-based feedback control. The array of patterned cell colonies allows for rapid selection of numerous stably transfected clonal cell lines located on the same culture well, conferring a significant advantage over slower and labor-intensive traditional methods. In addition to plasmid integration, this methodology can be seamlessly combined with CRISPR/Cas9 gene editing, paving the way for advanced cell engineering.
MeSH term(s)	Animals ; CRISPR-Cas Systems/genetics ; Cell Line ; Electroporation/methods ; Gene Editing/methods ; Humans ; Plasmids/genetics ; Transfection
Language	English
Publishing date	2018-02-12
Publishing country	Germany
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2168935-0
ISSN	1613-6829 ; 1613-6810
ISSN (online)	1613-6829
ISSN	1613-6810
DOI	10.1002/smll.201702495
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Article: Complément dans les glomérulonéphrites humaines primitives.

Lemaitre, V

LARC medical

1981 Volume 1, Issue 2, Page(s) 60, 62, 64

Title translation	The complement in primary human glomerulonephritis.
MeSH term(s)	Acute Disease ; Complement System Proteins/analysis ; Glomerulonephritis/etiology ; Glomerulonephritis/immunology ; Humans ; Streptococcal Infections/complications
Chemical Substances	Complement System Proteins (9007-36-7)
Language	French
Publishing date	1981-11
Publishing country	France
Document type	Journal Article
ZDB-ID	604511-x
ISSN	0242-9462
ISSN	0242-9462
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.B 2530: Show issues

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Article ; Online: Débranchement de fistule artérioveineuse : rationaliser la prise en charge du risque hémorragique.

Delfosse, Fleur / Boyer, Julien / Lemaitre, Vincent / Inghels, Yves

Nephrologie & therapeutique

2012 Volume 8, Issue 1, Page(s) 23–34

Abstract: During the disconnection of the arteriovenous fistulas, the nursing staff manages a hemorrhagic risk function of various factors requiring the use of an adapted haemostatic treatment. In front of numerous available references, it seemed interesting to us ...

Title translation	Disconnection of arteriovenous fistula: standardize the coverage of the hemorragic risk.
Abstract	During the disconnection of the arteriovenous fistulas, the nursing staff manages a hemorrhagic risk function of various factors requiring the use of an adapted haemostatic treatment. In front of numerous available references, it seemed interesting to us to standardize the coverage of this risk within the service of hemodialysis. We realized an audit to analyze the practices of disconnections and estimate the context of use of the haemostatic agents. A review of the consumptions as well as a medical economic study were realized on the haemostatic agents used in dialysis. A revaluation of the practices after 6 months was programmed to validate the institution of the protocol. Fifty observed disconnections brought to light numerous critical points. A decision tree was established to direct the choice of the haemostatic method. The importance of the direct compression in first intention was reminded and data sheets were realized. Two absorbable bandages were deleted by the allocation and an alginate of class III was subjected to name specific prescription. The assessment in 6 months proves the support of the teams to this new protocol by the impact on the consumptions and the follow-up of the decision tree during the disconnections. This study allowed us to harmonize a common act and to put at the disposal of the nursing only bandage haemostatics indicated in the disconnection of the arteriovenous fistulas. The spread good use was a major axis of improvement of the practices as well as a help for the integration of the new procedure.
MeSH term(s)	Aged ; Arteriovenous Shunt, Surgical ; Decision Trees ; Female ; Hemorrhage/prevention & control ; Hemostatic Techniques ; Humans ; Male ; Practice Guidelines as Topic ; Records as Topic ; Renal Dialysis ; Risk
Language	French
Publishing date	2012-02
Publishing country	France
Document type	English Abstract ; Journal Article
ZDB-ID	2229575-6
ISSN	1872-9177 ; 1769-7255
ISSN (online)	1872-9177
ISSN	1769-7255
DOI	10.1016/j.nephro.2011.04.004
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Article: A review of oxygen-17 solid-state NMR of organic materials--towards biological applications.

Lemaître, V / Smith, M E / Watts, A

Solid state nuclear magnetic resonance

2004 Volume 26, Issue 3-4, Page(s) 215–235

Abstract: 17O solid state NMR of organic materials is developing rapidly. This article provides a snapshot of the current state of development of this field. The NMR techniques and enrichment protocols that are driving this progress are outlined. The (17)O ... ...

Abstract	17O solid state NMR of organic materials is developing rapidly. This article provides a snapshot of the current state of development of this field. The NMR techniques and enrichment protocols that are driving this progress are outlined. The (17)O parameters derived from solid-state NMR experiments are summarized and the structural sensitivity of the approach to effects such as hydrogen bonding highlighted. The prospects and challenges for (17)O solid-state NMR of biomolecules are discussed.
MeSH term(s)	Chemistry, Organic ; Magnetic Resonance Spectroscopy/methods ; Organic Chemistry Phenomena ; Oxygen Isotopes
Chemical Substances	Oxygen Isotopes
Language	English
Publishing date	2004-11
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1122088-0
ISSN	1527-3326 ; 0926-2040
ISSN (online)	1527-3326
ISSN	0926-2040
DOI	10.1016/j.ssnmr.2004.04.004
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