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  1. Article ; Online: Molecular Identification of Enteric Viruses in Domestic Animals in Northeastern Gabon, Central Africa

    Bohou Kombila, Linda / N’dilimabaka, Nadine / Garcia, Déborah / Rieu, Océane / Engone Ondo, Jéordy Dimitri / Ndong Mebaley, Telstar / Boundenga, Larson / Fritz, Matthieu / Lenguiya, Léadisaelle Hosanna / Maganga, Gael Darren / Leroy, Eric M. / Becquart, Pierre / Mombo, Illich Manfred

    Animals. 2023 Aug. 03, v. 13, no. 15

    2023  

    Abstract: Astroviruses (AstVs), enteroviruses (EVs), and caliciviruses (CaVs) infect several vertebrate taxa. Transmitted through the fecal–oral route, these enteric viruses are highly resistant and can survive in the environment, thereby increasing their zoonotic ...

    Abstract Astroviruses (AstVs), enteroviruses (EVs), and caliciviruses (CaVs) infect several vertebrate taxa. Transmitted through the fecal–oral route, these enteric viruses are highly resistant and can survive in the environment, thereby increasing their zoonotic potential. Here, we screened for AstVs, EVs, and CaVs to investigate the role of domestic animals in the emergence of zoonoses, because they are situated at the human/wildlife interface, particularly in rural forested areas in Central Africa. Rectal swabs were obtained from 123 goats, 41 sheep, and 76 dogs in 10 villages located in northeastern Gabon. Extracted RNA reverse-transcribed into cDNA was used to detect AstVs, EVs, and CaVs by amplification of the RNA-dependent RNA polymerase (RdRp), or capsid protein (VP1) gene using PCR. A total of 23 samples tested positive, including 17 goats for AstVs, 2 goats, 2 sheep, 1 dog for EVs, and 1 dog for CaVs. Phylogenetic analyses revealed that AstV RdRp sequences clustered with sheep-, goat-, or bovine-related AstVs. In addition, one goat and two sheep VP1 sequences clustered with caprine/ovine-related Evs within the Enterovirus G species, and the CaV was a canine vesivirus. However, human-pathogenic Evs, EV-B80 and EV-C99, were detected in goats and dogs, raising questions on the maintenance of viruses able to infect humans.
    Keywords Astroviridae ; Enterovirus G ; RNA ; RNA-directed RNA polymerase ; Vesivirus ; coat proteins ; dogs ; genes ; goats ; humans ; phylogeny ; sheep ; wildlife ; zoonoses ; Gabon
    Language English
    Dates of publication 2023-0803
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13152512
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Absence of Coronavirus RNA in Faecal Samples from Wild Primates in Gabon, Central Africa.

    Mombo, Illich Manfred / Rieu, Océane / Fritz, Matthieu / Boundenga, Larson / Mebaley, Telstar Ndong / Mbou-Boutambe, Clark / Lenguiya, Léadisaelle Hosanna / Maganga, Gael Darren / Rougeron, Virginie / Prugnolle, Franck / Thomas, Fredéric / Leroy, Eric M

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 10

    Abstract: Coronaviruses (CoVs, ...

    Abstract Coronaviruses (CoVs,
    Language English
    Publishing date 2023-10-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12101272
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Evidence for circulation of Rift Valley fever virus in wildlife and domestic animals in a forest environment in Gabon, Central Africa.

    Becquart, Pierre / Bohou Kombila, Linda / Mebaley, Telstar Ndong / Paupy, Christophe / Garcia, Déborah / Nesi, Nicolas / Olive, Marie-Marie / Vanhomwegen, Jessica / Boundenga, Larson / Mombo, Illich Manfred / Piro-Mégy, Camille / Fritz, Matthieu / Lenguiya, Léadisaelle Hosanna / Ar Gouilh, Meriadeg / Leroy, Eric M / N'Dilimabaka, Nadine / Cêtre-Sossah, Catherine / Maganga, Gael Darren

    PLoS neglected tropical diseases

    2024  Volume 18, Issue 3, Page(s) e0011756

    Abstract: Rift Valley fever (RVF) is a mosquito-borne viral zoonosis caused by the Rift Valley fever virus (RVFV) that can infect domestic and wild animals. Although the RVFV transmission cycle has been well documented across Africa in savanna ecosystems, little ... ...

    Abstract Rift Valley fever (RVF) is a mosquito-borne viral zoonosis caused by the Rift Valley fever virus (RVFV) that can infect domestic and wild animals. Although the RVFV transmission cycle has been well documented across Africa in savanna ecosystems, little is known about its transmission in tropical rainforest settings, particularly in Central Africa. We therefore conducted a survey in northeastern Gabon to assess RVFV circulation among wild and domestic animals. Among 163 wildlife samples tested using RVFV-specific RT-qPCR, four ruminants belonging to subfamily Cephalophinae were detected positive. The phylogenetic analysis revealed that the four RVFV sequences clustered together with a virus isolated in Namibia within the well-structured Egyptian clade. A cross-sectional survey conducted on sheep, goats and dogs living in villages within the same area determined the IgG RVFV-specific antibody prevalence using cELISA. Out of the 306 small ruminants tested (214 goats, 92 sheep), an overall antibody prevalence of 15.4% (95% CI [11.5-19.9]) was observed with a higher rate in goats than in sheep (20.1% versus 3.3%). RVFV-specific antibodies were detected in a single dog out of the 26 tested. Neither age, sex of domestic animals nor season was found to be significant risk factors of RVFV occurrence. Our findings highlight sylvatic circulation of RVFV for the first time in Gabon. These results stress the need to develop adequate surveillance plan measures to better control the public health threat of RVFV.
    MeSH term(s) Animals ; Sheep ; Dogs ; Rift Valley fever virus ; Animals, Domestic ; Animals, Wild ; Gabon/epidemiology ; Cross-Sectional Studies ; Ecosystem ; Phylogeny ; Rift Valley Fever ; Ruminants ; Goats ; Antibodies, Viral ; Forests ; Seroepidemiologic Studies
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2735
    ISSN (online) 1935-2735
    ISSN 1935-2735
    DOI 10.1371/journal.pntd.0011756
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Whole-Genome Characterization of SARS-CoV-2 Reveals Simultaneous Circulation of Three Variants and a Putative Recombination (20B/20H) in Pets, Brazzaville, Republic of the Congo.

    Lenguiya, Léadisaelle Hosanna / Fritz, Matthieu / De Fonclare, Daphné de Riols / Corbet, Sandrine / Becquart, Pierre / Mbou, Christophe / Nguie, Ruben Junias / Mouellet, Wivine Salva / Demboux, Jordy Exaucé Lyelet / N'kaya-Tobi / Issamou Mayengue, Pembe / Koukouikila-Koussounda, Félix / Ar Gouilh, Meriadeg / Leroy, Eric M / Niama, Fabien Roch

    Viruses

    2023  Volume 15, Issue 4

    Abstract: Following the emergence of SARS-CoV-2, cases of pets infected with variants circulating among humans were reported. In order to evaluate the occurrence of SARS-CoV-2 circulation among pets in the Republic of the Congo, we conducted a ten-month study of ... ...

    Abstract Following the emergence of SARS-CoV-2, cases of pets infected with variants circulating among humans were reported. In order to evaluate the occurrence of SARS-CoV-2 circulation among pets in the Republic of the Congo, we conducted a ten-month study of dogs and cats living in COVID-19-positive households in Brazzaville and neighboring localities. Real-time PCR and the Luminex platform were used to detect SARS-CoV-2 RNA and antibodies to SARS-CoV-2 RBD and S proteins, respectively. Our results show for the first time the simultaneous circulation of several variants of SARS-CoV-2, including viruses from clades 20A and 20H and a putative recombinant variant between viruses from clades 20B and 20H. We found a high seroprevalence of 38.6%, with 14% of tested pets positive for SARS-CoV-2 RNA. Thirty-four percent of infected pets developed mild clinical signs, including respiratory and digestive signs, and shed the virus for about one day to two weeks. These results highlight the potential risk of SARS-CoV-2 interspecies transmission and the benefits of a "One Health" approach that includes SARS-CoV-2 diagnosis and surveillance of viral diversity in pets. This approach aims to prevent transmission to surrounding wildlife as well as spillback to humans.
    MeSH term(s) Animals ; Cats ; Dogs ; Humans ; SARS-CoV-2/genetics ; Congo/epidemiology ; COVID-19/epidemiology ; COVID-19/veterinary ; COVID-19 Testing ; Cat Diseases ; Dog Diseases/diagnosis ; Dog Diseases/epidemiology ; RNA, Viral/genetics ; Seroepidemiologic Studies ; Recombination, Genetic
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2023-04-09
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15040933
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Molecular Identification of Enteric Viruses in Domestic Animals in Northeastern Gabon, Central Africa.

    Bohou Kombila, Linda / N'dilimabaka, Nadine / Garcia, Déborah / Rieu, Océane / Engone Ondo, Jéordy Dimitri / Ndong Mebaley, Telstar / Boundenga, Larson / Fritz, Matthieu / Lenguiya, Léadisaelle Hosanna / Maganga, Gael Darren / Leroy, Eric M / Becquart, Pierre / Mombo, Illich Manfred

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 15

    Abstract: Astroviruses (AstVs) ...

    Abstract Astroviruses (AstVs)
    Language English
    Publishing date 2023-08-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13152512
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Early Circulation of SARS-CoV-2, Congo, 2020.

    Bonguili, Novy Charel Bobouaka / Fritz, Matthieu / Lenguiya, Leadisaelle Hosanna / Mayengue, Pembe Issamou / Koukouikila-Koussounda, Félix / Dossou-Yovo, Louis Régis / Badzi, Cynthia Nkoua / Leroy, Eric M / Niama, Fabien R

    Emerging infectious diseases

    2022  Volume 28, Issue 4, Page(s) 878–880

    Abstract: To determine when severe acute respiratory syndrome coronavirus 2 arrived in Congo, we retrospectively antibody tested 937 blood samples collected during September 2019-February 2020. Seropositivity significantly increased from 1% in December 2019 to 5.3% ...

    Abstract To determine when severe acute respiratory syndrome coronavirus 2 arrived in Congo, we retrospectively antibody tested 937 blood samples collected during September 2019-February 2020. Seropositivity significantly increased from 1% in December 2019 to 5.3% in February 2020, before the first officially reported case in March 2020, suggesting unexpected early virus circulation.
    MeSH term(s) COVID-19 ; Congo/epidemiology ; Humans ; Retrospective Studies ; SARS-CoV-2
    Language English
    Publishing date 2022-02-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2804.212476
    Database MEDical Literature Analysis and Retrieval System OnLINE

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