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  1. Article ; Online: Occult HBV Infection in Patients Infected by HIV or HCV: Comparison between HBV-DNA and Two Assays for HBsAg.

    Meschi, Silvia / Mizzoni, Klizia / Leoni, Bruno Daniele / Galli, Claudio / Garbuglia, Anna Rosa / Belladonna, Stefano / Girardi, Enrico / Maggi, Fabrizio / The Hbsagn Study Group

    Viruses

    2024  Volume 16, Issue 3

    Abstract: We investigated the frequency and serological correlates of occult hepatitis B virus infection (OBI) and the potential impact of a highly sensitive assay for HBsAg in subjects infected by human immunodeficiency virus (HIV) or hepatitis C virus (HCV), who ...

    Abstract We investigated the frequency and serological correlates of occult hepatitis B virus infection (OBI) and the potential impact of a highly sensitive assay for HBsAg in subjects infected by human immunodeficiency virus (HIV) or hepatitis C virus (HCV), who are also at risk for hepatitis B virus (HBV) infection, often in an occult form. Samples from 499 patients with HIV, all HBsAg negative and anti-HBc positive, and 137 patients with HCV were tested for HBV-DNA, anti-HBc, anti-HBs, and HBsAg by a conventional and highly sensitive assay. HBV biomarkers were detected in 71.5% of HCV-RNA-positive, with a higher prevalence of cases positive only for anti-HBc in patients with HCV than in those with HIV. HBV-DNA was detectable in 0.6% of HIV-positive and 7.3% of HCV-RNA-positive patients. Among patients with HCV, four were positive for HBsAg and negative for HBV-DNA, bringing the rate of HBV-active infection in this group to 10.2%. Active HBV infection was not related to gender or specific patterns of HBV biomarkers but was higher in HCV patients coinfected by HIV compared to those infected only by HCV. Monitoring patients at high risk for HBV infection and reactivation may require testing for both HBV-DNA and HBsAg.
    MeSH term(s) Humans ; Hepatitis B virus/genetics ; Hepacivirus/genetics ; Hepatitis B Surface Antigens ; DNA, Viral ; HIV/genetics ; HIV Infections ; Hepatitis B/diagnosis ; Hepatitis B/epidemiology ; Hepatitis C/diagnosis ; Hepatitis C/epidemiology ; Hepatitis B, Chronic ; Hepatitis B Antibodies ; Prevalence ; Biomarkers ; RNA
    Chemical Substances Hepatitis B Surface Antigens ; DNA, Viral ; Hepatitis B Antibodies ; Biomarkers ; RNA (63231-63-0)
    Language English
    Publishing date 2024-03-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v16030412
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Neutralizing Antibodies against SARS-CoV-2 Beta and Omicron Variants Inhibition Comparison after BNT162b2 mRNA Booster Doses with a New PETIA sVNT Assay.

    Fogolari, Marta / Leoni, Bruno Daniele / De Cesaris, Marina / Italiano, Rita / Davini, Flavio / Miccoli, Ginevra Azzurra / Donati, Daniele / Clerico, Luigi / Stanziale, Andrea / Savini, Giovanni / Petrosillo, Nicola / Ciccozzi, Massimo / Sommella, Lorenzo / Riva, Elisabetta / Fazii, Paolo / Angeletti, Silvia

    Diagnostics (Basel, Switzerland)

    2023  Volume 13, Issue 5

    Abstract: Background: Monitoring antibody response following SARS-CoV-2 vaccination is strategic, and neutralizing antibodies represent the gold standard. The neutralizing response to Beta and Omicron VOCs was evaluated versus the gold standard by a new ... ...

    Abstract Background: Monitoring antibody response following SARS-CoV-2 vaccination is strategic, and neutralizing antibodies represent the gold standard. The neutralizing response to Beta and Omicron VOCs was evaluated versus the gold standard by a new commercial automated assay.
    Methods: Serum samples from 100 healthcare workers from the Fondazione Policlinico Universitario Campus Biomedico and the Pescara Hospital were collected. IgG levels were determined by chemiluminescent immunoassay (Abbott Laboratories, Wiesbaden, Germany) and serum neutralization assay as the gold standard. Moreover, a new commercial immunoassay, the PETIA test Nab (SGM, Rome, Italy), was used for neutralization evaluation. Statistical analysis was performed with R software, version 3.6.0.
    Results: Anti-SARS-CoV-2 IgG titers decayed during the first ninety days after the vaccine second dose. The following booster dose significantly (
    Conclusion: This study correlates vaccine-induced IgG expression and neutralizing activity using a new PETIA assay, suggesting its usefulness for SARS-CoV2 infection management.
    Language English
    Publishing date 2023-02-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics13050889
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Serum iPTH range in a reference population: From an integrated approach to vitamin D prevalence impact evaluation.

    Minieri, Marilena / Leoni, Bruno Daniele / Bellincampi, Lorenza / Bajo, Daniela / Agnoli, Alessia / De Angelis, Anna Maria / Pieri, Massimo / Equitani, Francesco / Rossi, Vincenzo / Valente, Filippa / Pignalosa, Stefano / Terrinoni, Alessandro / Bernardini, Sergio

    Clinica chimica acta; international journal of clinical chemistry

    2021  Volume 521, Page(s) 1–8

    Abstract: Background: The iPTH upper reference limit (URL) reported by our laboratory provider (Abbott Laboratories) at Tor Vergata University Hospital was evaluated by internal verification procedures as not representative of our population and resulting as ... ...

    Abstract Background: The iPTH upper reference limit (URL) reported by our laboratory provider (Abbott Laboratories) at Tor Vergata University Hospital was evaluated by internal verification procedures as not representative of our population and resulting as underestimated. In this study, a new reference interval has been investigated and established by comparing a direct and an indirect method based on a statistical reduction from results stored in the laboratory database.
    Methods: For reference interval calculation from the healthy population, we analyzed a cohort of 100 blood donors (84% males and 16% females) screened with no bone-related and malabsorption diseases. We analyzed a cohort of 495 patients retrieved from more than 800 iPTH results by excluding subjects with pathological measurement for calcium, phosphorus, and creatinine for the reference interval evaluation. Patients with vitamin D results were included in the analysis. Vitamin D sufficiency status during the period from January to September 2020 was also evaluated by investigating 3,050 patients.
    Results: The iPTH reference interval of a healthy blood donor population was measured as 25.2-109.1 pg/mL (2.7-11.6 pmol/L) at 2.5 and 97.5 distribution percentile. The iPTH reference interval from data stored in the laboratory database was 19.3-112.5 pg/mL (2.0-11.9 pmol/L). Furthermore, 60% of the whole population had prevalently insufficient vitamin D concentration (<30 ng/dL; <75 nmol/L). The impact of vitamin D concentration on the iPTH reference interval was measured for insufficient vitamin D (<30 ng/dL; <75 nmol/L) as 15.2-127.7 pg/mL (1.6-13.5 pmol/L), desirable vitamin D (30-40 ng/ml; 75-100 nmol/L) as 25.6-105 pg/mL (2.7-10.7 pmol/L) and optimal vitamin D (>40 ng/ml; >100 nmol/L) as 26.2-89.2 pg/mL (2.8-9.4 pmol/L), respectively.
    Conclusions: The URL reported in manufacturer datasheets likely refers to a normal population with non-pathological vitamin D levels. On the contrary, the considered population was mostly vitamin D insufficient, resulting in a URL shift. On this basis, we suggest describing in medical reports the iPTH range for vitamin D deficiency for diagnosis of primary hyperparathyroidism even when a specific vitamin D request is lacking. On the other hand, reporting optimal vitamin D-based iPTH reference interval could be clinically relevant in supplemented patients as a marker of treatment efficacy.
    MeSH term(s) Calcium ; Female ; Humans ; Male ; Parathyroid Hormone ; Prevalence ; Vitamin D ; Vitamin D Deficiency
    Chemical Substances Parathyroid Hormone ; Vitamin D (1406-16-2) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-06-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2021.06.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.173: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  4. Article: Differentiation of Caco-2 cells requires both transcriptional and post-translational down-regulation of Myc

    Leoni, Bruno Daniele / Natoli, Manuela / Nardella, Marta / Bucci, Barbara / Zucco, Flavia / D'Agnano, Igea / Felsani, Armando

    Differentiation. 2012 Mar., v. 83, no. 3

    2012  

    Abstract: Caco-2 cancer cell line is widely used to reproduce in vitro the differentiation of absorptive enterocytes of human intestinal epithelium. This cell line, when cultured over confluence for 21 days, spontaneously undergoes cell cycle arrest and ... ...

    Abstract Caco-2 cancer cell line is widely used to reproduce in vitro the differentiation of absorptive enterocytes of human intestinal epithelium. This cell line, when cultured over confluence for 21 days, spontaneously undergoes cell cycle arrest and differentiates with the formation of a polarized enterocyte-like monolayer. During this process, Myc protein is completely down-regulated, as occurs in normal enterocytes. Caco-2 cells differ from normal enterocytes for mutations of APC and β-catenin genes, factors known to be involved in the transcriptional control of MYC gene during enterocyte differentiation. In this paper, we investigated how Myc regulation could be achieved during Caco-2 differentiative process, notwithstanding the APC and β-catenin mutations. We highlighted the post translational regulation of Myc protein as one of the essential mechanisms that allows the exit from cell cycle and onset of differentiation of Caco-2 cells. Moreover, we found a strong correlation between Myc protein downregulation and the expression of the transcription factor Cdx2, suggesting the existence of a regulative link between these two proteins.
    Keywords cell cycle ; enterocytes ; gene expression regulation ; genes ; human cell lines ; humans ; mutation ; proteins ; transcription (genetics) ; transcription factors ; translation (genetics)
    Language English
    Dates of publication 2012-03
    Size p. 116-127.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 184540-8
    ISSN 1432-0436 ; 0301-4681
    ISSN (online) 1432-0436
    ISSN 0301-4681
    DOI 10.1016/j.diff.2011.10.005
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 75/707: Show issues

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  5. Article ; Online: Differentiation of Caco-2 cells requires both transcriptional and post-translational down-regulation of Myc.

    Leoni, Bruno Daniele / Natoli, Manuela / Nardella, Marta / Bucci, Barbara / Zucco, Flavia / D'Agnano, Igea / Felsani, Armando

    Differentiation; research in biological diversity

    2012  Volume 83, Issue 3, Page(s) 116–127

    Abstract: Caco-2 cancer cell line is widely used to reproduce in vitro the differentiation of absorptive enterocytes of human intestinal epithelium. This cell line, when cultured over confluence for 21 days, spontaneously undergoes cell cycle arrest and ... ...

    Abstract Caco-2 cancer cell line is widely used to reproduce in vitro the differentiation of absorptive enterocytes of human intestinal epithelium. This cell line, when cultured over confluence for 21 days, spontaneously undergoes cell cycle arrest and differentiates with the formation of a polarized enterocyte-like monolayer. During this process, Myc protein is completely down-regulated, as occurs in normal enterocytes. Caco-2 cells differ from normal enterocytes for mutations of APC and β-catenin genes, factors known to be involved in the transcriptional control of MYC gene during enterocyte differentiation. In this paper, we investigated how Myc regulation could be achieved during Caco-2 differentiative process, notwithstanding the APC and β-catenin mutations. We highlighted the post translational regulation of Myc protein as one of the essential mechanisms that allows the exit from cell cycle and onset of differentiation of Caco-2 cells. Moreover, we found a strong correlation between Myc protein downregulation and the expression of the transcription factor Cdx2, suggesting the existence of a regulative link between these two proteins.
    MeSH term(s) Adenomatous Polyposis Coli Protein/genetics ; Adenomatous Polyposis Coli Protein/metabolism ; CDX2 Transcription Factor ; Caco-2 Cells ; Cell Cycle/genetics ; Cell Differentiation ; Enterocytes/cytology ; Enterocytes/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Humans ; Intestines/cytology ; Intestines/growth & development ; Mutation ; Proto-Oncogene Proteins c-myc/genetics ; Proto-Oncogene Proteins c-myc/metabolism ; RNA Interference ; beta Catenin/genetics ; beta Catenin/metabolism
    Chemical Substances APC protein, human ; Adenomatous Polyposis Coli Protein ; CDX2 Transcription Factor ; CDX2 protein, human ; Homeodomain Proteins ; MYC protein, human ; Proto-Oncogene Proteins c-myc ; beta Catenin
    Language English
    Publishing date 2012-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 184540-8
    ISSN 1432-0436 ; 0301-4681
    ISSN (online) 1432-0436
    ISSN 0301-4681
    DOI 10.1016/j.diff.2011.10.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1170: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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