Article ; Online: New Detection Platform for Screening Bacteria in Liquid Samples.
2021 Volume 11, Issue 5
Abstract: The development of sensitive methods for the determination of potential bacterial contamination is of upmost importance for environmental monitoring and food safety. In this study, we present a new method combining a fast pre-enrichment step using a ... ...
Abstract | The development of sensitive methods for the determination of potential bacterial contamination is of upmost importance for environmental monitoring and food safety. In this study, we present a new method combining a fast pre-enrichment step using a microporous cryogel and a detection and identification step using antimicrobial peptides (AMPs) and labelled antibodies, respectively. The experimental method consists of: (i) the capture of large amounts of bacteria from liquid samples by using a highly porous and functionalized cryogel; (ii) the detection and categorisation of Gram-positive and Gram-negative bacteria by determining their affinities toward a small set of AMPs; and (iii) the identification of the bacterial strain by using labelled detection antibodies. As proof of concept, the assessment of the three steps of the analysis was performed by using |
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MeSH term(s) | Bacillus ; Bacteria/isolation & purification ; Environmental Monitoring ; Escherichia coli ; Food Safety ; Water Microbiology |
Language | English |
Publishing date | 2021-05-01 |
Publishing country | Switzerland |
Document type | Journal Article |
ZDB-ID | 2662125-3 |
ISSN | 2079-6374 ; 2079-6374 |
ISSN (online) | 2079-6374 |
ISSN | 2079-6374 |
DOI | 10.3390/bios11050142 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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