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Article ; Online: New Detection Platform for Screening Bacteria in Liquid Samples.

La Spina, Rita / António, Diana C / Bombera, Radoslaw / Lettieri, Teresa / Lequarré, Anne-Sophie / Colpo, Pascal / Valsesia, Andrea

Biosensors

2021 Volume 11, Issue 5

Abstract: The development of sensitive methods for the determination of potential bacterial contamination is of upmost importance for environmental monitoring and food safety. In this study, we present a new method combining a fast pre-enrichment step using a ... ...

Abstract	The development of sensitive methods for the determination of potential bacterial contamination is of upmost importance for environmental monitoring and food safety. In this study, we present a new method combining a fast pre-enrichment step using a microporous cryogel and a detection and identification step using antimicrobial peptides (AMPs) and labelled antibodies, respectively. The experimental method consists of: (i) the capture of large amounts of bacteria from liquid samples by using a highly porous and functionalized cryogel; (ii) the detection and categorisation of Gram-positive and Gram-negative bacteria by determining their affinities toward a small set of AMPs; and (iii) the identification of the bacterial strain by using labelled detection antibodies. As proof of concept, the assessment of the three steps of the analysis was performed by using
MeSH term(s)	Bacillus ; Bacteria/isolation & purification ; Environmental Monitoring ; Escherichia coli ; Food Safety ; Water Microbiology
Language	English
Publishing date	2021-05-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios11050142
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: One Health security lessons from a year-long webinar series on international COVID-19 response.

Kennedy, Caroline R M / de Bruin, Yuri Bruinen / Lequarré, Anne-Sophie / Ackerman, Rebecca T / Luster, Jill / Tsang, Tiffany M / McInturff, Kari D / Carter, Cassandra P / Pilch, Richard

One health outlook

2022 Volume 4, Issue 1, Page(s) 15

Abstract: Following the principles outlined by the Global Outbreak Alert and Response Network, the Federal Bureau of Investigation's International Biosecurity and Prevention Forum, the European Commission's Joint Research Centre, and the Middlebury Institute of ... ...

Abstract	Following the principles outlined by the Global Outbreak Alert and Response Network, the Federal Bureau of Investigation's International Biosecurity and Prevention Forum, the European Commission's Joint Research Centre, and the Middlebury Institute of International Studies' James Martin Center for Nonproliferation Studies cohosted a webinar series from April 2020 to January 2021 on COVID-19 management across Africa, Europe, and North America. We provide here an overview of the webinar series and discuss how lessons learned during the COVID-19 pandemic and debated during the webinars can be used to bridge One Health with biological threat-driven health security. This report can be used to inform recommendations for future One Health security approaches to strengthen global capacity and multidisciplinary cooperation.
Language	English
Publishing date	2022-10-08
Publishing country	England
Document type	Journal Article
ISSN	2524-4655
ISSN (online)	2524-4655
DOI	10.1186/s42522-022-00071-0
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Book ; Article ; Online: Initial impacts of global risk mitigation measures taken during the combatting of the COVID-19 pandemic

BRUINEN DE BRUIN YURI / LEQUARRE ANNE SOPHIE / MCCOURT JOSEPHINE / CLEVESTIG PETER / PIGAZZANI FILIPO / ZARE JEDDI MARYAM / COLOMBO CLAUDIO / GOULART DE MEDEIROS MARGARIDA

2020

Abstract: This paper presents an analysis of risk mitigation measures taken by countries around the world facing the current COVID-19 outbreak. In light of the current pandemic the authors collated and clustered (using harmonised terminology) the risk mitigation ... ...

Abstract	This paper presents an analysis of risk mitigation measures taken by countries around the world facing the current COVID-19 outbreak. In light of the current pandemic the authors collated and clustered (using harmonised terminology) the risk mitigation measures taken around the globe in the combat to contain, and since March 11 2020, to limiting the spread of the SARS-CoV-2 virus known to cause the Coronavirus disease 2019 (COVID-19). This overview gathers lessons learnt, provides an update on the current knowledge for authorities, sectors and first responders on the effectiveness and may allow enhanced prevention, preparedness and response for future outbreaks. Various measures such as mobility restrictions, physical distancing, hygienic measures, socio economic restrictions, communication and international support mechanisms have been clustered and are reviewed in terms of the nature of the actions taken and their qualitative early-perceived impact. At the time of writing, it is still too premature to express the quantitative effectiveness of each risk mitigation cluster, but it seems that the best mitigation results are reported when applying a combination of voluntary and enforceable measures. JRC.E.7-Knowledge for Security and Migration
Keywords	covid19
Subject code	710
Language	ENG
Publisher	ELSEVIER SCIENCE BV
Publishing country	eu
Document type	Book ; Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Initial impacts of global risk mitigation measures taken during the combatting of the COVID-19 pandemic.

Bruinen de Bruin, Yuri / Lequarre, Anne-Sophie / McCourt, Josephine / Clevestig, Peter / Pigazzani, Filippo / Zare Jeddi, Maryam / Colosio, Claudio / Goulart, Margarida

Safety science

2020 Volume 128, Page(s) 104773

Abstract	This paper presents an analysis of risk mitigation measures taken by countries around the world facing the current COVID-19 outbreak. In light of the current pandemic the authors collated and clustered (using harmonised terminology) the risk mitigation measures taken around the globe in the combat to contain, and since March 11, 2020, to limit the spread of the SARS-CoV-2 virus known to cause the Coronavirus disease 2019 (COVID-19). This overview gathers lessons learnt, providing an update on the current knowledge for authorities, sectors and first responders on the effectiveness of said measures, and may allow enhanced prevention, preparedness and response for future outbreaks. Various measures such as mobility restrictions, physical distancing, hygienic measures, socio-economic restrictions, communication and international support mechanisms have been clustered and are reviewed in terms of the nature of the actions taken and their qualitative early-perceived impact. At the time of writing, it is still too premature to express the quantitative effectiveness of each risk mitigation cluster, but it seems that the best mitigation results are reported when applying a combination of voluntary and enforceable measures.
Keywords	covid19
Language	English
Publishing date	2020-04-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1074634-1
ISSN	1879-1042 ; 0925-7535
ISSN (online)	1879-1042
ISSN	0925-7535
DOI	10.1016/j.ssci.2020.104773
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Book ; Online: Standardisation needs for COVID-19. Scoping exercise on potential standards gaps carried out among JRC scientists. Putting Science into Standards (PSIS)

Jenet, Andreas / Nik, Samira / Mian, Livia / Schmidtler, Stella-Zoe / Annunziato, Alessandro / Marin-Ferrer, Montserrat / McCourt, Josephine / Lequarre, Anne Sophie / Ganesh, Ashok / Taucer, Fabio

2021

Abstract: The Joint Research Centre in collaboration with the European standardization bodies CEN and CENELEC launched a scoping exercise on standardization needs in response to COVID-19 and future pandemics. The purpose of the exercise was to identify ongoing ... ...

Abstract	The Joint Research Centre in collaboration with the European standardization bodies CEN and CENELEC launched a scoping exercise on standardization needs in response to COVID-19 and future pandemics. The purpose of the exercise was to identify ongoing harmonization initiatives, as was well as further standardization needs in relevant sectors such as artisanal reusable face masks, medical face masks, and social distancing in closed public or commercial spaces. An overview of already ongoing standardization activities relevant to COVID-19 in Spain and Italy illustrate, although fragmented and partially complete, the importance of standardization in key sectors for combatting pandemics, such as in health, social, safety and security. This report informs colleagues in European institutions and Member States about the crucial role standardization plays in the common efforts to overcome the COVID-19 pandemic. Examples include potential inputs to the drafting of guidelines, methods and or interoperability standards. Finally, the report also provides practical examples of agile standardization activities and deliverables that have the potential to enable the EU to respond more effectively and multilaterally to future crises. With this report we aim to raise awareness about the opportunities that standardization and harmonization can bring in the context of the COVID-19 pandemic. Comment: 24 pages, European Commission, Brussels, 2021, JRC121514
Keywords	Quantitative Biology - Other Quantitative Biology
Subject code	300
Publishing date	2021-07-15
Publishing country	us
Document type	Book ; Online
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Article ; Online: Genome wide association study of 40 clinical measurements in eight dog breeds.

Momozawa, Yukihide / Merveille, Anne-Christine / Battaille, Géraldine / Wiberg, Maria / Koch, Jørgen / Willesen, Jakob Lundgren / Proschowsky, Helle Friis / Gouni, Vassiliki / Chetboul, Valérie / Tiret, Laurent / Fredholm, Merete / Seppälä, Eija H / Lohi, Hannes / Georges, Michel / Lequarré, Anne-Sophie

Scientific reports

2020 Volume 10, Issue 1, Page(s) 6520

Abstract: The domestic dog represents an ideal model for identifying susceptibility genes, many of which are shared with humans. In this study, we investigated the genetic contribution to individual differences in 40 clinically important measurements by a genome- ... ...

Abstract	The domestic dog represents an ideal model for identifying susceptibility genes, many of which are shared with humans. In this study, we investigated the genetic contribution to individual differences in 40 clinically important measurements by a genome-wide association study (GWAS) in a multinational cohort of 472 healthy dogs from eight breeds. Meta-analysis using the binary effects model after breed-specific GWAS, identified 13 genome-wide significant associations, three of them showed experimental-wide significant associations. We detected a signal at chromosome 13 for the serum concentration of alanine aminotransferase (ALT) in which we detected four breed-specific signals. A large proportion of the variance of ALT (18.1-47.7%) was explained by this locus. Similarly, a single SNP was also responsible for a large proportion of the variance (6.8-78.4%) for other measurements such as fructosamine, stress during physical exam, glucose, and morphometric measurements. The genetic contribution of single variant was much larger than in humans. These findings illustrate the importance of performing meta-analysis after breed-specific GWAS to reveal the genetic contribution to individual differences in clinically important measurements, which would lead to improvement of veterinary medicine.
MeSH term(s)	Alanine Transaminase/genetics ; Animals ; Breeding ; Chromosomes/genetics ; Dog Diseases/genetics ; Dog Diseases/pathology ; Dogs ; Fructosamine/genetics ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Humans ; Male ; Phenotype ; Polymorphism, Single Nucleotide/genetics
Chemical Substances	Fructosamine (4429-04-3) ; Alanine Transaminase (EC 2.6.1.2)
Language	English
Publishing date	2020-04-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-63457-y
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Article: Initial impacts of global risk mitigation measures taken during the combatting of the COVID-19 pandemic

Bruinen de Bruin, Yuri / Lequarre, Anne-Sophie / McCourt, Josephine / Clevestig, Peter / Pigazzani, Filippo / Zare Jeddi, Maryam / Colosio, Claudio / Goulart, Margarida

Saf Sci

Abstract	This paper presents an analysis of risk mitigation measures taken by countries around the world facing the current COVID-19 outbreak. In light of the current pandemic the authors collated and clustered (using harmonised terminology) the risk mitigation measures taken around the globe in the combat to contain, and since March 11 2020, to limit the spread of the SARS-CoV-2 virus known to cause the Coronavirus disease 2019 (COVID-19). This overview gathers lessons learnt, providing an update on the current knowledge for authorities, sectors and first responders on the effectiveness of said measures, and may allow enhanced prevention, preparedness and response for future outbreaks. Various measures such as mobility restrictions, physical distancing, hygienic measures, socio-economic restrictions, communication and international support mechanisms have been clustered and are reviewed in terms of the nature of the actions taken and their qualitative early-perceived impact. At the time of writing, it is still too premature to express the quantitative effectiveness of each risk mitigation cluster, but it seems that the best mitigation results are reported when applying a combination of voluntary and enforceable measures.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #66211
Database	COVID19

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Article ; Online: Initial impacts of global risk mitigation measures taken during the combatting of the COVID-19 pandemic

Bruinen de Bruin, Yuri / Lequarre, Anne-Sophie / McCourt, Josephine / Clevestig, Peter / Pigazzani, Filippo / Zare Jeddi, Maryam / Colosio, Claudio / Goulart, Margarida

Safety science, 128:104773

2020

Abstract	This paper presents an analysis of risk mitigation measures taken by countries around the world facing the current COVID-19 outbreak. In light of the current pandemic the authors collated and clustered (using harmonised terminology) the risk mitigation measures taken around the globe in the combat to contain, and since March 11, 2020, to limit the spread of the SARS-CoV-2 virus known to cause the Coronavirus disease 2019 (COVID-19). This overview gathers lessons learnt, providing an update on the current knowledge for authorities, sectors and first responders on the effectiveness of said measures, and may allow enhanced prevention, preparedness and response for future outbreaks. Various measures such as mobility restrictions, physical distancing, hygienic measures, socio-economic restrictions, communication and international support mechanisms have been clustered and are reviewed in terms of the nature of the actions taken and their qualitative early-perceived impact. At the time of writing, it is still too premature to express the quantitative effectiveness of each risk mitigation cluster, but it seems that the best mitigation results are reported when applying a combination of voluntary and enforceable measures.
Keywords	COVID-19 ; Public health ; Risk mitigation measures ; Mitigation impact ; SARS-CoV-2 ; Coronavirus ; covid19
Language	English
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Initial impacts of global risk mitigation measures taken during the combatting of the COVID-19 pandemic

Bruinen de Bruin, Yuri / Lequarre, Anne-Sophie / McCourt, Josephine / Clevestig, Peter / Pigazzani, Filippo / Zare Jeddi, Maryam / Colosio, Claudio / Goulart, Margarida

Safety Science

2020 Volume 128, Page(s) 104773

Keywords	Public Health, Environmental and Occupational Health ; Safety Research ; Safety, Risk, Reliability and Quality ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	1074634-1
ISSN	1879-1042 ; 0925-7535
ISSN (online)	1879-1042
ISSN	0925-7535
DOI	10.1016/j.ssci.2020.104773
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Effects of polyadenylation inhibition on meiosis progression in relation to the polyadenylation status of cyclins A2 and B1 during in vitro maturation of bovine oocytes.

Traverso, Juan M / Donnay, Isabelle / Lequarre, Anne-Sophie

Molecular reproduction and development

2005 Volume 71, Issue 1, Page(s) 107–114

Abstract: The control of protein synthesis during maturation in oocytes is mainly exerted through cytoplasmic polyadenylation of stored mRNAs. We first analyzed the polyadenylation status of cyclins A2 and B1 during in vitro maturation (IVM) of bovine oocytes, ... ...

Abstract	The control of protein synthesis during maturation in oocytes is mainly exerted through cytoplasmic polyadenylation of stored mRNAs. We first analyzed the polyadenylation status of cyclins A2 and B1 during in vitro maturation (IVM) of bovine oocytes, using Rapid Amplification of cDNA Ends-Polyadenylation Technique (RACE-PAT). An inconstant elongation of the poly(A) tail was observed for cyclin A2 transcripts after maturation, while a constant lengthening was observed for cyclin B1, occurring during the first 12 hr of incubation. We then evaluated the effects of the polyadenylation inhibitor 3'-deoxyadenosine (3'-dA), on polyadenylation and nuclear maturation. The presence of 0.02 mM 3'-dA during the whole incubation period or from 6 hr after its beginning completely prevented meiosis progression in 100% of the oocytes. Polyadenylation of cyclin B1 was also completely prevented when 3'-dA was added at 0 hr, and greatly reduced when added at 6 hr. When 3'-dA was added at 12 hr, around metaphase I (MI), 46.9% of the oocytes have reached metaphase II (MII, vs. 78.8% in the control group) at 24 hr. The use of the same concentration of 3'-deoxyguanosine (3'-dG), that impairs transcription but not polyadenylation, did not affect cyclins polyadenylation, nor nuclear maturation, whatever was the timing of addition. These results suggest that the polyadenylation of cyclin B1 could be related to the first peak of activity of MPF, occurring around MI (10-12 hr after the onset of the maturation period). They also show that, in our culture conditions, inhibition of polyadenylation prevents meiosis progression, especially up to the MI stage, while inhibition of transcription does not.
MeSH term(s)	Animals ; Cattle ; Cell Nucleus/drug effects ; Cell Nucleus/metabolism ; Cells, Cultured ; Cyclin A/metabolism ; Cyclin B/genetics ; Cyclin B/metabolism ; Cyclin B1 ; Deoxyadenosines/pharmacology ; Female ; In Vitro Techniques ; Kinetics ; Meiosis/drug effects ; Oocytes/cytology ; Oocytes/drug effects ; Oocytes/metabolism ; Poly A/genetics ; Polyadenylation/drug effects ; Transcription, Genetic/drug effects ; Transcription, Genetic/genetics
Chemical Substances	Cyclin A ; Cyclin B ; Cyclin B1 ; Deoxyadenosines ; Poly A (24937-83-5) ; cordycepin (GZ8VF4M2J8)
Language	English
Publishing date	2005-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	20321-x
ISSN	1098-2795 ; 1040-452X
ISSN (online)	1098-2795
ISSN	1040-452X
DOI	10.1002/mrd.20247
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