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Artikel ; Online: Mutation of SOCS2 induces structural and functional changes in mammary development.

Ivanova, Elitsa / Hue-Beauvais, Cathy / Castille, Johan / Laubier, Johann / Le Guillou, Sandrine / Aujean, Etienne / Lecardonnel, Jerome / Lebrun, Laura / Jaffrezic, Florence / Rousseau-Ralliard, Delphine / Péchoux, Christine / Letheule, Martine / Foucras, Gilles / Charlier, Madia / Le Provost, Fabienne

Development (Cambridge, England)

2024 Band 151, Heft 6

Abstract: Lactation is an essential process for mammals. In sheep, the R96C mutation in suppressor of cytokine signaling 2 (SOCS2) protein is associated with greater milk production and increased mastitis sensitivity. To shed light on the involvement of R96C ... ...

Abstract	Lactation is an essential process for mammals. In sheep, the R96C mutation in suppressor of cytokine signaling 2 (SOCS2) protein is associated with greater milk production and increased mastitis sensitivity. To shed light on the involvement of R96C mutation in mammary gland development and lactation, we developed a mouse model carrying this mutation (SOCS2KI/KI). Mammary glands from virgin adult SOCS2KI/KI mice presented a branching defect and less epithelial tissue, which were not compensated for in later stages of mammary development. Mammary epithelial cell (MEC) subpopulations were modified, with mutated mice having three times as many basal cells, accompanied by a decrease in luminal cells. The SOCS2KI/KI mammary gland remained functional; however, MECs contained more lipid droplets versus fat globules, and milk lipid composition was modified. Moreover, the gene expression dynamic from virgin to pregnancy state resulted in the identification of about 3000 differentially expressed genes specific to SOCS2KI/KI or control mice. Our results show that SOCS2 is important for mammary gland development and milk production. In the long term, this finding raises the possibility of ensuring adequate milk production without compromising animal health and welfare.
Mesh-Begriff(e)	Animals ; Female ; Mice ; Pregnancy ; Epithelial Cells/metabolism ; Lactation/genetics ; Mammary Glands, Animal/metabolism ; Milk/metabolism ; Mutation/genetics
Chemische Substanzen	Socs2 protein, mouse
Sprache	Englisch
Erscheinungsdatum	2024-03-14
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	90607-4
ISSN	1477-9129 ; 0950-1991
ISSN (online)	1477-9129
ISSN	0950-1991
DOI	10.1242/dev.202332
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Transcription of rRNA in early mouse embryos promotes chromatin reorganization and expression of major satellite repeats.

Chebrout, Martine / Koné, Maïmouna Coura / Jan, Habib U / Cournut, Marie / Letheule, Martine / Fleurot, Renaud / Aguirre-Lavin, Tiphaine / Peynot, Nathalie / Jouneau, Alice / Beaujean, Nathalie / Bonnet-Garnier, Amélie

Journal of cell science

2022 Band 135, Heft 6

Abstract: During the first cell cycles of early development, the chromatin of the embryo is highly reprogrammed while the embryonic genome starts its own transcription. The spatial organization of the genome is an important process that contributes to regulating ... ...

Abstract	During the first cell cycles of early development, the chromatin of the embryo is highly reprogrammed while the embryonic genome starts its own transcription. The spatial organization of the genome is an important process that contributes to regulating gene transcription in time and space. It has, however, been poorly studied in the context of early embryos. To study the cause-and-effect link between transcription and spatial organization in embryos, we focused on ribosomal genes, which are silent initially but start to be transcribed in 2-cell mouse embryos. We demonstrated that ribosomal sequences and early unprocessed rRNAs are spatially organized in a very particular manner between 2-cell and 16-cell stage. By using drugs that interfere with ribosomal DNA transcription, we showed that this organization - which is totally different in somatic cells - depends on an active transcription of ribosomal genes and induces a unique chromatin environment that favors transcription of major satellite sequences once the 4-cell stage has been reached.
Mesh-Begriff(e)	Animals ; Chromatin/genetics ; Chromatin/metabolism ; DNA, Ribosomal/genetics ; Embryo, Mammalian/metabolism ; Mice ; RNA, Ribosomal/genetics ; RNA, Ribosomal/metabolism ; Ribosomes/metabolism ; Transcription, Genetic
Chemische Substanzen	Chromatin ; DNA, Ribosomal ; RNA, Ribosomal
Sprache	Englisch
Erscheinungsdatum	2022-03-16
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.258798
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Transient Changes of Metabolism at the Pronuclear Stage in Mice Influences Skeletal Muscle Phenotype in Adulthood.

Bertrand-Gaday, Christelle / Letheule, Martine / Blanchet, Emilie / Vernus, Barbara / Pessemesse, Laurence / Bonnet-Garnier, Amélie / Bonnieu, Anne / Casas, François

International journal of molecular sciences

2020 Band 21, Heft 19

Abstract: Skeletal muscle has a remarkable plasticity, and its phenotype is strongly influenced by hormones, transcription factors, and physical activity. However, whether skeletal phenotype can be oriented or not during early embryonic stages has never been ... ...

Abstract	Skeletal muscle has a remarkable plasticity, and its phenotype is strongly influenced by hormones, transcription factors, and physical activity. However, whether skeletal phenotype can be oriented or not during early embryonic stages has never been investigated. Here, we report that pyruvate as the only source of carbohydrate in the culture medium of mouse one cell stage embryo influenced the establishment of the muscular phenotype in adulthood. We found that pyruvate alone induced changes in the contractile phenotype of the skeletal muscle in a sexually dependent manner. For male mice, a switch to a more glycolytic phenotype was recorded, whereas, in females, the pyruvate induced a switch to a more oxidative phenotype. In addition, the influence of pyruvate on the contractile phenotypes was confirmed in two mouse models of muscle hypertrophy: the well-known myostatin deficient mouse (Mstn-/-) and a mouse carrying a specific deletion of p43, a mitochondrial triiodothyronine receptor. Finally, to understand the link between these adult phenotypes and the early embryonic period, we assessed the levels of two histone H3 post-translational modifications in presence of pyruvate alone just after the wave of chromatin reprogramming specific of the first cell cycle. We showed that H3K4 acetylation level was decreased in Mstn-/- 2-cell embryos, whereas no difference was found for H3K27 trimethylation level, whatever the genotype. These findings demonstrate for the first time that changes in the access of energy substrate during the very first embryonic stage can induce a precocious orientation of skeletal muscle phenotype in adulthood.
Mesh-Begriff(e)	Acetylation ; Animals ; Carbohydrate Metabolism/genetics ; Cytokines/genetics ; Disease Models, Animal ; Embryo, Mammalian ; Embryonic Development/genetics ; Female ; Genotype ; Glycolysis/genetics ; Hypertrophy/genetics ; Hypertrophy/metabolism ; Hypertrophy/pathology ; Male ; Mice ; Mitochondria/metabolism ; Muscle Contraction/genetics ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/pathology ; Myostatin/genetics ; Oxidation-Reduction ; Phenotype ; Pyruvic Acid/metabolism
Chemische Substanzen	Aimp1 protein, mouse ; Cytokines ; Mstn protein, mouse ; Myostatin ; Pyruvic Acid (8558G7RUTR)
Sprache	Englisch
Erscheinungsdatum	2020-09-29
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21197203
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Pressurized intra-peritoneal aerosol chemotherapy (PIPAC): increased intraperitoneal pressure does not affect distribution patterns but leads to deeper penetration depth of doxorubicin in a sheep model.

Mimouni, Myriam / Richard, Christophe / Adenot, Pierre / Letheule, Martine / Tarrade, Anne / Sandra, Olivier / Dahirel, Michèle / Lilin, Thomas / Lecuelle, Benoit / Gélin, Valérie / Cohen, Julien / Fauconnier, Arnaud / Vialard, François / Huchon, Cyrille / Chavatte-Palmer, Pascale

BMC cancer

2021 Band 21, Heft 1, Seite(n) 461

Abstract: Background: Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) is an innovative treatment against peritoneal carcinomatosis. Doxorubicin is a common intra-venous chemotherapy used for peritoneal carcinomatosis and for PIPAC. This study evaluated ... ...

Abstract	Background: Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) is an innovative treatment against peritoneal carcinomatosis. Doxorubicin is a common intra-venous chemotherapy used for peritoneal carcinomatosis and for PIPAC. This study evaluated the impact of increased PIPAC intraperitoneal pressure on the distribution and cell penetration of doxorubicin in a sheep model. Methods: Doxorubicin was aerosolized using PIPAC into the peritoneal cavity of 6 ewes (pre-alpes breed): N = 3 with 12 mmHg intraperitoneal pressure ("group 12") and N = 3 with 20 mmHg ("group 20"). Samples from peritoneum (N = 6), ovarian (N = 1), omentum (N = 1) and caecum (N = 1) were collected for each ewe. The number of doxorubicin positive cells was determined using the ratio between doxorubicine fluorescence-positive cell nuclei (DOXO+) over total number of DAPI positive cell nuclei (DAPI+). Penetration depth (μm) was defined as the distance between the luminal surface and the location of the deepest DOXO+ nuclei over the total number of cell nuclei that were stained with DAPI. Penetration depth (μm) was defined as the distance between the luminal surface and the location of the deepest DOXO+ nuclei. Results: DOXO+ nuclei were identified in 87% of samples. All omental samples, directly localized in front of the nebulizer head, had 100% DOXO+ nuclei whereas very few nuclei were DOXO+ for caecum. Distribution patterns were not different between the two groups but penetration depth in ovary and caecum samples was significantly deeper in group 20. Conclusions: This study showed that applying a higher intra-peritoneal pressure during PIPAC treatment leads to a deeper penetration of doxorubicin in ovarian and caecum but does not affect distribution patterns.
Mesh-Begriff(e)	Aerosols ; Animals ; Antibiotics, Antineoplastic/administration & dosage ; Antibiotics, Antineoplastic/analysis ; Antibiotics, Antineoplastic/pharmacokinetics ; Cecum/chemistry ; Cecum/metabolism ; Cell Nucleus/chemistry ; Doxorubicin/administration & dosage ; Doxorubicin/analysis ; Doxorubicin/pharmacokinetics ; Drug Delivery Systems/methods ; Female ; Omentum/chemistry ; Omentum/metabolism ; Ovary/chemistry ; Ovary/metabolism ; Peritoneal Neoplasms/drug therapy ; Peritoneal Neoplasms/metabolism ; Peritoneum/chemistry ; Peritoneum/metabolism ; Pressure ; Sheep ; Tissue Distribution
Chemische Substanzen	Aerosols ; Antibiotics, Antineoplastic ; Doxorubicin (80168379AG)
Sprache	Englisch
Erscheinungsdatum	2021-04-26
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2041352-X
ISSN	1471-2407 ; 1471-2407
ISSN (online)	1471-2407
ISSN	1471-2407
DOI	10.1186/s12885-021-07955-w
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: DNAJC2 is required for mouse early embryonic development

Helary, Louise / Baraige, Fabienne / Beauvallet, Christian / Blanquet, Véronique / Castille, Johan / Charles, Mathieu / Duchesne, Amandine / Jaffrezic, Florence / Laubier, Johann / Letheule, Martine / Moazami-Goudarzi, Katayoun / Passet, Bruno / Tamzini, Mayssa / Vaiman, Anne / Vilotte, Jean-Luc

Biochemical and biophysical research communications. 2019 Aug. 13, v. 516, no. 1

2019

Abstract: DNAJC2 protein, also known as ZRF1 or MPP11, acts both as chaperone and as chromatin regulator. It is involved in stem cell differentiation and its expression is associated with various cancer malignancies. However, the role of Dnajc2 gene during mouse ... ...

Abstract	DNAJC2 protein, also known as ZRF1 or MPP11, acts both as chaperone and as chromatin regulator. It is involved in stem cell differentiation and its expression is associated with various cancer malignancies. However, the role of Dnajc2 gene during mouse embryogenesis has not been assessed so far. To this aim, we invalidated Dnajc2 gene in FVB/Nj mice using the CrispR/Cas9 approach. We showed that this invalidation leads to the early post-implantation lethality of the nullizygous embryos. Furthermore, using siRNAs against Dnajc2 in mouse 1-cell embryos, we showed that maternal Dnajc2 mRNAs may allow for the early preimplantation development of these embryos. Altogether, these data demonstrate for the first time the requirement of DNAJC2 for early mouse embryogenesis.
Schlagwörter	cell differentiation ; chromatin ; CRISPR-Cas systems ; death ; embryo (animal) ; embryogenesis ; genes ; messenger RNA ; mice ; neoplasms ; small interfering RNA ; stem cells
Sprache	Englisch
Erscheinungsverlauf	2019-0813
Umfang	p. 258-263.
Erscheinungsort	Elsevier Inc.
Dokumenttyp	Artikel
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2019.06.009
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: DNAJC2 is required for mouse early embryonic development.

Helary, Louise / Castille, Johan / Passet, Bruno / Vaiman, Anne / Beauvallet, Christian / Jaffrezic, Florence / Charles, Mathieu / Tamzini, Mayssa / Baraige, Fabienne / Letheule, Martine / Laubier, Johann / Moazami-Goudarzi, Katayoun / Vilotte, Jean-Luc / Blanquet, Véronique / Duchesne, Amandine

Biochemical and biophysical research communications

2019 Band 516, Heft 1, Seite(n) 258–263

Abstract	DNAJC2 protein, also known as ZRF1 or MPP11, acts both as chaperone and as chromatin regulator. It is involved in stem cell differentiation and its expression is associated with various cancer malignancies. However, the role of Dnajc2 gene during mouse embryogenesis has not been assessed so far. To this aim, we invalidated Dnajc2 gene in FVB/Nj mice using the CrispR/Cas9 approach. We showed that this invalidation leads to the early post-implantation lethality of the nullizygous embryos. Furthermore, using siRNAs against Dnajc2 in mouse 1-cell embryos, we showed that maternal Dnajc2 mRNAs may allow for the early preimplantation development of these embryos. Altogether, these data demonstrate for the first time the requirement of DNAJC2 for early mouse embryogenesis.
Mesh-Begriff(e)	Animals ; CRISPR-Cas Systems ; DNA-Binding Proteins/genetics ; Embryo Implantation ; Embryo Loss/genetics ; Embryo, Mammalian/embryology ; Embryo, Mammalian/metabolism ; Embryonic Development ; Female ; Gene Deletion ; Gene Expression Regulation, Developmental ; Mice/embryology ; Mice/genetics ; Molecular Chaperones/genetics ; Pregnancy ; RNA-Binding Proteins/genetics
Chemische Substanzen	DNA-Binding Proteins ; Dnajc2 protein, mouse ; Molecular Chaperones ; RNA-Binding Proteins
Sprache	Englisch
Erscheinungsdatum	2019-06-21
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2019.06.009
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Mise en évidence d’une transition endothéliale/mésenchymateuse dans les lésions vasculaires pulmonaires d’hypertension artérielle pulmonaire par microscopie corrélative

Ranchoux, Benoît / Rucker-Martin, Catherine / Chat, Sophie / Letheule, Martine / Perros, Frédéric / Péchoux, Christine

12. Journées de formation du RCCM (Réseau des Centres Communs de Microscopie), Poitiers, FRA, 2013-05-29-2013-05-31

Abstract: L'hypertension artérielle pulmonaire résulte de l'obturation des artères pulmonaires de diamètre inférieur à 500 μm. Par microscopie corrélative, nous avons pu démontrer que cette obturation était la conséquence d'une trans-différenciation des cellules ... ...

Abstract	L'hypertension artérielle pulmonaire résulte de l'obturation des artères pulmonaires de diamètre inférieur à 500 μm. Par microscopie corrélative, nous avons pu démontrer que cette obturation était la conséquence d'une trans-différenciation des cellules endothéliales en cellules mésenchymateuses.
Sprache	fra
Dokumenttyp	Artikel
Datenquelle	AGRIS - International Information System for the Agricultural Sciences and Technology

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Artikel: Mise en évidence d’une transition endothéliale/mésenchymateuse dans les lésions vasculaires pulmonaires d’hypertension artérielle pulmonaire par microscopie corrélative

Ranchoux, Benoît / Rucker-Martin, Catherine / Chat, Sophie / Letheule, Martine / Perros, Frédéric / Péchoux, Christine

12. Journées de formation du RCCM (Réseau des Centres Communs de Microscopie), Poitiers, FRA, 2013-05-29-2013-05-31

Abstract	L'hypertension artérielle pulmonaire résulte de l'obturation des artères pulmonaires de diamètre inférieur à 500 μm. Par microscopie corrélative, nous avons pu démontrer que cette obturation était la conséquence d'une trans-différenciation des cellules endothéliales en cellules mésenchymateuses.
Sprache	fra
Dokumenttyp	Artikel
Datenquelle	AGRIS - International Information System for the Agricultural Sciences and Technology

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