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  1. Article ; Online: Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors.

    Procházková, Jiřina / Slavík, Josef / Bouchal, Jan / Levková, Monika / Hušková, Zlata / Ehrmann, Jiří / Ovesná, Petra / Kolář, Zdeněk / Skalický, Pavel / Straková, Nicol / Zapletal, Ondřej / Kozubík, Alois / Hofmanová, Jiřina / Vondráček, Jan / Machala, Miroslav

    Biochimica et biophysica acta. Molecular and cell biology of lipids

    2020  Volume 1865, Issue 9, Page(s) 158742

    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Colorectal Neoplasms/metabolism ; Epithelial Cell Adhesion Molecule/metabolism ; Female ; Galactosyltransferases/genetics ; Humans ; Male ; Middle Aged ; Sphingolipids/metabolism
    Chemical Substances EPCAM protein, human ; Epithelial Cell Adhesion Molecule ; Sphingolipids ; Galactosyltransferases (EC 2.4.1.-)
    Language English
    Publishing date 2020-05-21
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2618 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2618 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbalip.2020.158742
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  2. Article ; Online: Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells.

    Hofmanová, Jiřina / Slavík, Josef / Ciganek, Miroslav / Ovesná, Petra / Tylichová, Zuzana / Karasová, Martina / Zapletal, Ondřej / Straková, Nicol / Procházková, Jiřina / Bouchal, Jan / Kolář, Zdeněk / Ehrmann, Jiří / Levková, Monika / Hušková, Zlatka / Skalický, Pavel / Kozubík, Alois / Machala, Miroslav / Vondráček, Jan

    International journal of molecular sciences

    2021  Volume 22, Issue 13

    Abstract: The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor ... ...

    Abstract The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM
    MeSH term(s) Adenocarcinoma/enzymology ; Adenocarcinoma/genetics ; Adenocarcinoma/metabolism ; Aged ; Colonic Neoplasms/enzymology ; Colonic Neoplasms/genetics ; Colonic Neoplasms/metabolism ; Epithelial Cells/enzymology ; Epithelial Cells/metabolism ; Fatty Acid Desaturases/genetics ; Fatty Acid Desaturases/metabolism ; Fatty Acid Elongases/genetics ; Fatty Acid Elongases/metabolism ; Fatty Acid Synthases/genetics ; Fatty Acid Synthases/metabolism ; Fatty Acids/metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Lipid Metabolism ; Lipidomics ; Lipogenesis ; Male ; Phospholipids/metabolism ; Stearoyl-CoA Desaturase/genetics ; Stearoyl-CoA Desaturase/metabolism
    Chemical Substances ELOVL5 protein, human ; Fatty Acids ; Phospholipids ; Fatty Acid Desaturases (EC 1.14.19.-) ; Stearoyl-CoA Desaturase (EC 1.14.19.1) ; FADS2 protein, human (EC 1.14.19.3) ; Fatty Acid Elongases (EC 2.3.1.-) ; Fatty Acid Synthases (EC 2.3.1.85)
    Language English
    Publishing date 2021-06-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22136650
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells

    Steigerová, Jana / Rárová, Lucie / Oklešt’ková, Jana / Křížová, Kateřina / Levková, Monika / Šváchová, Michaela / Kolář, Zdeněk / Strnad, Miroslav

    Food and chemical toxicology. 2012 Nov., v. 50, no. 11

    2012  

    Abstract: Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28- ... ...

    Abstract Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G₁ blocks in LNCaP cells accompanied by reductions in cyclin D₁, CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G₂/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B₁. Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs.
    Keywords DNA ; Western blotting ; antineoplastic agents ; apoptosis ; brassinosteroids ; cell cycle ; cell growth ; cyclins ; flow cytometry ; fluorescent antibody technique ; humans ; neoplasm cells ; prostatic neoplasms ; toxicology
    Language English
    Dates of publication 2012-11
    Size p. 4068-4076.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2012.08.031
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4035: Show issues

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  4. Article ; Online: Brassinosteroids cause cell cycle arrest and apoptosis of human breast cancer cells.

    Steigerová, Jana / Oklešťková, Jana / Levková, Monika / Rárová, Lucie / Kolář, Zdeněk / Strnad, Miroslav

    Chemico-biological interactions

    2010  Volume 188, Issue 3, Page(s) 487–496

    Abstract: Brassinosteroids (BRs) are plant hormones that appear to be ubiquitous in both lower and higher plants. Recently, we published the first evidence that some natural BRs induce cell growth inhibitory responses in several human cancer cell lines without ... ...

    Abstract Brassinosteroids (BRs) are plant hormones that appear to be ubiquitous in both lower and higher plants. Recently, we published the first evidence that some natural BRs induce cell growth inhibitory responses in several human cancer cell lines without affecting normal non-tumor cell growth (BJ fibroblasts). The aim of the study presented here was to examine the mechanism of the antiproliferative activity of the natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in human hormone-sensitive and -insensitive (MCF-7 and MDA-MB-468, respectively) breast cancer cell lines. The effects of 6, 12 and 24h treatments with 28-homoCS and 24-epiBL on cancer cells were surveyed using flow cytometry, Western blotting, TUNEL assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced blocks in the G(1) cell cycle phase. ER-α immunoreactivity was uniformly present in the nuclei of control MCF-7 cells, while cytoplasmic speckles of ER-α immunofluorescence appeared in BR-treated cells (IC(50), 24h). ER-β was relocated to the nuclei following 28-homoCS treatment and found predominantly at the periphery of the nuclei in 24-epiBL-treated cells after 24h of treatment. These changes were also accompanied by down-regulation of the ERs following BR treatment. In addition, BR application to breast cancer cells resulted in G(1) phase arrest. Furthermore, TUNEL staining and double staining with propidium iodide and acridine orange demonstrated the BR-mediated induction of apoptosis in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by the BRs in each cell line. The studied BRs seem to exert potent growth inhibitory effects via interactions with the cell cycle machinery, and they could be highly valuable leads for agents for managing breast cancer.
    MeSH term(s) Apoptosis/drug effects ; Brassinosteroids ; Breast Neoplasms/pathology ; Cell Cycle/drug effects ; Cell Cycle Proteins/metabolism ; Cell Line, Tumor ; Cholestanols/pharmacology ; Cholestanones/pharmacology ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Protein Transport/drug effects ; Receptors, Estrogen/metabolism ; Signal Transduction/drug effects ; Steroids, Heterocyclic/pharmacology
    Chemical Substances Brassinosteroids ; Cell Cycle Proteins ; Cholestanols ; Cholestanones ; Receptors, Estrogen ; Steroids, Heterocyclic ; homocastasterone (83509-42-6) ; brassinolide (Y9IQ1L53OX)
    Language English
    Publishing date 2010-12-05
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218799-1
    ISSN 1872-7786 ; 0009-2797
    ISSN (online) 1872-7786
    ISSN 0009-2797
    DOI 10.1016/j.cbi.2010.09.006
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 686: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article ; Online: Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells.

    Steigerová, Jana / Rárová, Lucie / Oklešt'ková, Jana / Křížová, Kateřina / Levková, Monika / Sváchová, Michaela / Kolář, Zdeněk / Strnad, Miroslav

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

    2012  Volume 50, Issue 11, Page(s) 4068–4076

    Abstract: Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28- ... ...

    Abstract Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G(1) blocks in LNCaP cells accompanied by reductions in cyclin D(1), CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G(2)/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B(1). Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs.
    MeSH term(s) Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects ; Brassinosteroids/pharmacology ; Cell Cycle/drug effects ; Cell Line, Tumor ; Cholestanones/pharmacology ; Cyclin D1/metabolism ; Cyclin-Dependent Kinase 4/metabolism ; Cyclin-Dependent Kinase 6/metabolism ; Drug Screening Assays, Antitumor ; Humans ; Male ; Prostatic Neoplasms/drug therapy ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/pathology ; Receptors, Androgen/metabolism ; Receptors, Estrogen/metabolism ; Steroids, Heterocyclic/pharmacology
    Chemical Substances Antineoplastic Agents, Phytogenic ; Brassinosteroids ; CCND1 protein, human ; Cholestanones ; Receptors, Androgen ; Receptors, Estrogen ; Steroids, Heterocyclic ; Cyclin D1 (136601-57-5) ; homocastasterone (83509-42-6) ; CDK4 protein, human (EC 2.7.11.22) ; CDK6 protein, human (EC 2.7.11.22) ; Cyclin-Dependent Kinase 4 (EC 2.7.11.22) ; Cyclin-Dependent Kinase 6 (EC 2.7.11.22) ; brassinolide (Y9IQ1L53OX)
    Language English
    Publishing date 2012-11
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2012.08.031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 529: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells

    Steigerová, Jana / Rárová, Lucie / Oklešt’ková, Jana / Křížová, Kateřina / Levková, Monika / Šváchová, Michaela / Kolář, Zdeněk / Strnad, Miroslav

    Food and chemical toxicology

    Volume v. 50,, Issue no. 1

    Abstract: Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28- ... ...

    Abstract Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the antiproliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G₁ blocks in LNCaP cells accompanied by reductions in cyclin D₁, CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G₂/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B₁. Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs.
    Keywords Western blotting ; apoptosis ; brassinosteroids ; toxicology ; antineoplastic agents ; cyclins ; flow cytometry ; humans ; cell cycle ; prostatic neoplasms ; neoplasm cells ; DNA ; fluorescent antibody technique ; cell growth
    Language English
    Document type Article
    ISSN 0278-6915
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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