Article ; Online: The ABCG2 Q141K hyperuricemia and gout associated variant illuminates the physiology of human urate excretion.
2020 Volume 11, Issue 1, Page(s) 2767
Abstract: The pathophysiological nature of the common ABCG2 gout and hyperuricemia associated variant Q141K (rs2231142) remains undefined. Here, we use a human interventional cohort study (ACTRN12615001302549) to understand the physiological role of ABCG2 and find ...
| Abstract | The pathophysiological nature of the common ABCG2 gout and hyperuricemia associated variant Q141K (rs2231142) remains undefined. Here, we use a human interventional cohort study (ACTRN12615001302549) to understand the physiological role of ABCG2 and find that participants with the Q141K ABCG2 variant display elevated serum urate, unaltered FEUA, and significant evidence of reduced extra-renal urate excretion. We explore mechanisms by generating a mouse model of the orthologous Q140K Abcg2 variant and find male mice have significant hyperuricemia and metabolic alterations, but only subtle alterations of renal urate excretion and ABCG2 abundance. By contrast, these mice display a severe defect in ABCG2 abundance and function in the intestinal tract. These results suggest a tissue specific pathobiology of the Q141K variant, support an important role for ABCG2 in urate excretion in both the human kidney and intestinal tract, and provide insight into the importance of intestinal urate excretion for serum urate homeostasis. |
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| MeSH term(s) | ATP Binding Cassette Transporter, Subfamily G, Member 2/genetics ; ATP Binding Cassette Transporter, Subfamily G, Member 2/metabolism ; Alleles ; Animals ; Disease Models, Animal ; Epithelium/metabolism ; Epithelium/pathology ; Glucose Transport Proteins, Facilitative/genetics ; Glucose Transport Proteins, Facilitative/metabolism ; Gout/genetics ; Gout/metabolism ; Gout/pathology ; Homeostasis ; Humans ; Hyperuricemia/metabolism ; Intestines/pathology ; Kidney/metabolism ; Kidney/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neoplasm Proteins ; Phenotype ; Uric Acid/blood ; Uric Acid/metabolism |
| Chemical Substances | ABCG2 protein, human ; ATP Binding Cassette Transporter, Subfamily G, Member 2 ; Abcg2 protein, mouse ; Glucose Transport Proteins, Facilitative ; Neoplasm Proteins ; SLC2A9 protein, human ; Uric Acid (268B43MJ25) |
| Language | English |
| Publishing date | 2020-06-02 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2553671-0 |
| ISSN | 2041-1723 ; 2041-1723 |
| ISSN (online) | 2041-1723 |
| ISSN | 2041-1723 |
| DOI | 10.1038/s41467-020-16525-w |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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