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Article ; Online: The protective effect of Metformin/Donepezil in diabetic mice brain: evidence from bioinformatics analysis and experiments.

Hou, Y / Li, F-Z

European review for medical and pharmacological sciences

2022 Volume 25, Issue 24, Page(s) 7668–7678

Abstract: Objective: To identify candidate differentially expressed genes (DEGs) and pathways in diabetic mice brain with metformin/donepezil, network pharmacology analysis was used and verified by experiments.: Materials and methods: We analyzed GSE62013 ... ...

Abstract	Objective: To identify candidate differentially expressed genes (DEGs) and pathways in diabetic mice brain with metformin/donepezil, network pharmacology analysis was used and verified by experiments. Materials and methods: We analyzed GSE62013 microarray datasets derived from the Gene Expression Omnibus (GEO) database for diabetic brain. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using the Database. Subsequently, the protein-protein interaction network (PPI) and Cytoscape were used for visualizing the most significant module and hub genes. Metformin/donepezil were used to treat streptozotocin (STZ)-induced diabetic mice models. Blood glucose levels and Morris water maze test were measured. The apoptotic rate of diabetic brain tissue was analyzed using Annexin V/propidium iodide double staining. The levels of PI3K and AKT in the mice brain tissues were detected by Western blot. Results: DEGs included 214 up-regulated genes and 100 down-regulated genes in diabetic brain tissues of mice. The enriched GO functions were multicellular organism development, negative regulation of transcription from RNA polymerase II promoter, and extracellular region. The enriched pathways were PI3K-Akt signaling pathway, Linoleic acid metabolism and Arachidonic acid metabolism. Blood glucose levels and apoptosis were reduced in STZ-induced diabetic mice following metformin/donepezil treatment. Metformin/donepezil could reverse this neurocognitive deficiency. Protein levels of PI3K and AKT were significantly increased in STZ-induced diabetic mice. Conclusions: Overall, we proposed that 10 genes (Cdc20, Fbxo32, Igtp, Atg7, Fbxo15, Trim37, Psmb8, Ifi47, Asb12, and Asb5) that might be novel hub genes strongly associated with diabetic mice brain. Metformin/donepezil ameliorates STZ-induced brain injury by activating the PI3K/AKT pathway and alleviating apoptosis.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Brain/drug effects ; Brain/metabolism ; Cholinesterase Inhibitors/pharmacology ; Cholinesterase Inhibitors/therapeutic use ; Computational Biology ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/metabolism ; Donepezil/pharmacology ; Donepezil/therapeutic use ; Hypoglycemic Agents/pharmacology ; Hypoglycemic Agents/therapeutic use ; Male ; Metformin/pharmacology ; Metformin/therapeutic use ; Mice, Inbred C57BL ; Neuroprotective Agents/pharmacology ; Neuroprotective Agents/therapeutic use ; Nootropic Agents/pharmacology ; Nootropic Agents/therapeutic use ; Phosphatidylinositol 3-Kinases/metabolism ; Protein Interaction Maps ; Proto-Oncogene Proteins c-akt/metabolism ; Mice
Chemical Substances	Cholinesterase Inhibitors ; Hypoglycemic Agents ; Neuroprotective Agents ; Nootropic Agents ; Donepezil (8SSC91326P) ; Metformin (9100L32L2N) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1)
Language	English
Publishing date	2022-01-04
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_202112_27613
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Article: [Airway surface micro-environment homeostasis and pulmonary infection].

Wang, D / Li, F Z / Zhang, B

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases

2020 Volume 43, Issue 9, Page(s) 801–804

MeSH term(s)	Homeostasis ; Humans ; Respiratory Mucosa ; Respiratory Tract Infections
Language	Chinese
Publishing date	2020-09-07
Publishing country	China
Document type	Journal Article
ZDB-ID	1027965-9
ISSN	1001-0939
ISSN	1001-0939
DOI	10.3760/cma.j.cn112147-20191109-00746
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Article: [Novel frameshift mutations in SALL4 in two Chinese families with Okihiro syndrome].

Bai, Y / Wu, Q H / Li, F Z / Sun, Y / Wang, L / Jiao, Z H / Kong, X D

Zhonghua yi xue za zhi

2023 Volume 103, Issue 26, Page(s) 2006–2010

Abstract: In the present study, clinical manifestations of two Chinese Okihiro syndrome families were analyzed, and genetic detections were performed on the two probands by exome sequencing and verified by Sanger sequencing for family members to determine the ... ...

Abstract	In the present study, clinical manifestations of two Chinese Okihiro syndrome families were analyzed, and genetic detections were performed on the two probands by exome sequencing and verified by Sanger sequencing for family members to determine the biological pathogenesis. Prenatal diagnoses were provided for three high-risk fetuses. The affected members exhibited a wildly spectrum of phenotypes, including ultrasound abnormalities of skeletal system (radius deformity and abnormal posture), and cardiac system (persistent common arterial trunk and ventricular septal defect) in the prenatal period of family 1, the severe phenotypes (grossly shortened and deformed forearm, Duane's anomaly and hearing loss), and the mild ones (usually only thenar dysplasia, or short radius styloid process). Two
MeSH term(s)	Female ; Humans ; Pregnancy ; Deafness ; Duane Retraction Syndrome ; East Asian People ; Frameshift Mutation ; Transcription Factors/genetics
Chemical Substances	SALL4 protein, human ; Transcription Factors
Language	Chinese
Publishing date	2023-07-12
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	132513-9
ISSN	0376-2491
ISSN	0376-2491
DOI	10.3760/cma.j.cn112137-20221206-02577
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Article: [Posterior cerebral cortex atrophy with visual hallucinations and fluctuations: a case report].

Wang, M / Yin, X / Gao, Z B / Wang, W / Chen, T / Jiang, Z Y / Li, F Z / Wang, Z F

Zhonghua nei ke za zhi

2023 Volume 62, Issue 4, Page(s) 446–448

MeSH term(s)	Humans ; Cerebral Cortex ; Hallucinations/etiology ; Hallucinations/pathology ; Neurodegenerative Diseases/pathology ; Atrophy/complications ; Atrophy/pathology ; Magnetic Resonance Imaging
Language	Chinese
Publishing date	2023-03-24
Publishing country	China
Document type	Case Reports ; Journal Article
ZDB-ID	754223-9
ISSN	0578-1426
ISSN	0578-1426
DOI	10.3760/cma.j.cn112138-20220330-00229
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Article ; Online: Clinical study of the correlation between complement factor H polymorphism and age-related macular degeneration.

Dong, H T / Zhang, J X / Li, Q M / Li, F Z

Genetics and molecular research : GMR

2016 Volume 15, Issue 3

Abstract: This study aimed to investigate the correlation between age-related macular degeneration (AMD) of the liver-kidney yin-deficiency type and complement factor H (CFH) polymorphism, and to determine whether the C allele of the T1277C (Y402H) variant is a ... ...

Abstract	This study aimed to investigate the correlation between age-related macular degeneration (AMD) of the liver-kidney yin-deficiency type and complement factor H (CFH) polymorphism, and to determine whether the C allele of the T1277C (Y402H) variant is a risk factor for this condition. We performed a case-control investigation of 60 patients with liver-kidney yin-deficiency AMD and 60 normal control subjects. Peripheral blood was collected from each participant for DNA extraction. Following amplification by polymerase chain reaction, the DNA samples were sequenced, and polymorphism of the CFH gene was examined. Data were analyzed with the chi-square test, with P < 0.05 signifying statistical significance. The frequency of the C allele was significantly higher in the wet than in the dry AMD group (P = 0.044). In addition, the TC and CC genotypes were markedly more common in the former than in the control group (P = 0.013), and there was a significant difference in the distribution of the T and C alleles between wet AMD patients and control subjects (P < 0.05). Based on this, we conclude that liver-kidney yin-deficiency AMD is associated with the C allele and TC and CC genotypes of the CFH Y402H polymorphism. Among patients with this condition, CFH genotypes were normally distributed. The principal CFH genotypes that induce liver-kidney yin-deficiency AMD are the mutant homozygote CC and heterozygote TC forms. Moreover, C allele carriers are at higher risk of developing this disease.
MeSH term(s)	Aged ; Aged, 80 and over ; Alleles ; Complement Factor H/genetics ; Female ; Genetic Association Studies ; Genetic Predisposition to Disease ; Genotype ; Heterozygote ; Homozygote ; Humans ; Kidney/pathology ; Liver/pathology ; Macular Degeneration/genetics ; Macular Degeneration/pathology ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Yin Deficiency/genetics ; Yin Deficiency/pathology
Chemical Substances	Complement Factor H (80295-65-4)
Language	English
Publishing date	2016-09-16
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2114039-X
ISSN	1676-5680 ; 1676-5680
ISSN (online)	1676-5680
ISSN	1676-5680
DOI	10.4238/gmr.15038457
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Article: [Application of real-time image fusion technique in transjugular intrahepatic portosystemic shunt].

Zhang, W W / Ren, J Z / Zhou, P L / Ding, P X / Chen, P F / Li, F Z / Kuang, D L / Han, X W

Zhonghua yi xue za zhi

2019 Volume 99, Issue 45, Page(s) 3554–3557

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adult ; Aged ; Female ; Humans ; Hypertension, Portal ; Liver Cirrhosis ; Male ; Middle Aged ; Portal Vein ; Portasystemic Shunt, Transjugular Intrahepatic ; Retrospective Studies ; Treatment Outcome ; Young Adult
Language	Chinese
Publishing date	2019-11-01
Publishing country	China
Document type	Journal Article
ZDB-ID	132513-9
ISSN	0376-2491
ISSN	0376-2491
DOI	10.3760/cma.j.issn.0376-2491.2019.45.005
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Article: [Clinical value of emergency endovascular embolization in the interventional treatment for oral hemorrhage caused by carcinoma].

Tang, F / Chen, P F / Li, F Z / Kuang, D L / Wang, J X / Wan, L / Han, X W / Ren, J Z / Duan, X H

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology

2021 Volume 56, Issue 4, Page(s) 370–373

Abstract: To evaluate the clinical value of emergency endovascular embolization in the interventional treatment for oral hemorrhage caused by carcinoma, 32 patients with oral hemorrhage caused by carcinoma, who received emergency endovascular embolization due to ... ...

Abstract	To evaluate the clinical value of emergency endovascular embolization in the interventional treatment for oral hemorrhage caused by carcinoma, 32 patients with oral hemorrhage caused by carcinoma, who received emergency endovascular embolization due to unsatisfactory hemostatic effect of conventional conservative treatment in the First Affiliated Hospital of Zhengzhou University from January 2014 to December 2019, were included in this study and their clinical data, laboratory data and imaging information were retrospectively analyzed. There were 16 males and 16 females, aged (60.6±13.6) years (34-88 years). Technical successful rate of emergency endovascular embolization, immediate successful rate of controlling hemorrhage, blood pressure before and after operation, hemoglobin before and after operation, postoperative complications and recurrence rate of oral hemorrhage were statistically analyzed. Results showed that technical successful rate of operation and immediate successful rate of controlling oral hemorrhage are both 100% (32/32). Recurrent oral hemorrhage occurred in 4 patients (13%). The hemorrhagic shock symptoms of all patients were significantly improved after interventional therapy. After operation, local swelling happened in 34% (11/32) patients and intermittent local pain happened in 22% (7/32) within 24 hours; the swelling and the pain gradually disappeared from 2nd to 5th days. Mild complications of transient fever happened in 9% (3/32) patients and disappeared spontaneously in the short term. No serious complications such as blindness, cerebrovascular accident or central nervous system disturbance occurred in all patients after operations. During the whole follow-up period (1 to 12 months), a total of 8 patients died. The causes of death were progression and metastasis of carcinoma (
MeSH term(s)	Aged ; Carcinoma ; Embolization, Therapeutic ; Female ; Humans ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Oral Hemorrhage ; Retrospective Studies ; Treatment Outcome
Language	Chinese
Publishing date	2021-04-08
Publishing country	China
Document type	Journal Article
ZDB-ID	1027950-7
ISSN	1002-0098
ISSN	1002-0098
DOI	10.3760/cma.j.cn112144-20200603-00312
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Article: [Combination of B-type brain natriuretic peptide with left ventricular diastolic dysfunction for prediction of mortality in the patients with septic shock].

Hu, B C / Wang, Y J / Ge, W D / Li, F Z / Sun, R H

Zhonghua yi xue za zhi

2016 Volume 96, Issue 29, Page(s) 2295–2300

Abstract: Objective: To evaluate the prognostic value of combination of plasma brain natriuretic peptide(BNP) with the ratio of early diastolic mitral inflow velocity to early diastolic mitral annulus velocity(E/E') in the patients with septic shock.: Methods: ...

Abstract	Objective: To evaluate the prognostic value of combination of plasma brain natriuretic peptide(BNP) with the ratio of early diastolic mitral inflow velocity to early diastolic mitral annulus velocity(E/E') in the patients with septic shock. Methods: From June 2012 to December 2013 , 127 patients with septic shock were consecutively recruited and underwent trans-thoracic echocardiography examination within 6 h after admission to Intensive Care Unit(ICU), Zhejiang Provincial People's Hospital. Plasma BNP concentration was measured using ELISA method. All Clinical, laboratory, and survival data were prospectively collected. Results: Of 127 patients enrolled, mean values for age were(59.9±17.3) years and APACHE Ⅱ score(16.8±5.8), respectively. 95 patients(74.8%) took mechanical ventilation. 28- , 60-day mortality rate was 36.3% and 42.3%, respectively. Univariate Cox regression analysis showed that age, coronary artery disease, serum creatinine and lactate, plasma BNP, left ventricular ejection fraction(LVEF), E/E' and APACHE Ⅱ score were significantly(P≤0.05) associated with 60-day mortality. Multivariate analysis revealed that serum lactate, plasma BNP(χ(2)=9.4, P=0.002) , E/E'(χ(2)=4.89, P=0.02) and APACHE Ⅱ score(χ(2)=10.6, P=0.001) remained independent predictors for 60-day mortality. ROC curve analysis showed that the optimal plasma BNP and E/E' cutoff values identified were 338.8 pg/ml and 10.8, and the areas under ROC curve were 0.89(sensitivity: 83.7%; specificity: 81.4%)and 0.83(sensitivity: 76.7%; specificity: 72.9%)for 60-day mortality, respectively. In addition to plasma BNP and clinical predictors, the E/E' could provide in independent and incremental prognostic value of 60-day mortality(χ(2)=59.3 vs 47.8, P<0.001). Conclusion: Plasma BNP and E/E' are independent predictors for 60-day mortality, and combination of plasma BNP and E/E' could improve risk stratification in patients with septic shock.
MeSH term(s)	Cardiomyopathies ; Echocardiography ; Humans ; Intensive Care Units ; Middle Aged ; Mitral Valve ; Multivariate Analysis ; Natriuretic Peptide, Brain ; Prognosis ; ROC Curve ; Shock, Septic ; Ventricular Function, Left
Chemical Substances	Natriuretic Peptide, Brain (114471-18-0)
Language	Chinese
Publishing date	2016-08-02
Publishing country	China
Document type	Journal Article
ZDB-ID	132513-9
ISSN	0376-2491
ISSN	0376-2491
DOI	10.3760/cma.j.issn.0376-2491.2016.29.003
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Article: Clinical effect of intravitreous injection of triamcinolone acetonide in treating cystoid macular edema.

Yan, P S / Qian, C / Wan, G M / Wang, W Z / Dong, Y / Li, F Z

Journal of biological regulators and homeostatic agents

2016 Volume 30, Issue 1, Page(s) 205–210

Abstract: Cystoid macular edema (CME), a commonly seen sign for multiple fundus diseases, is able to induce visual deterioration. The incidence rate of CME is constantly increasing; however, the existing clinical treatments cannot achieve satisfactory curative ... ...

Abstract	Cystoid macular edema (CME), a commonly seen sign for multiple fundus diseases, is able to induce visual deterioration. The incidence rate of CME is constantly increasing; however, the existing clinical treatments cannot achieve satisfactory curative effects. To explore the curative effect of intravitreous injection of triamcinolone acetonide (TA) in treating CME, this study carried out a clinical test on 39 patients (42 eyes) from The First Affiliated Hospital of Zhengzhou University who developed CME induced by central retinal vein occlusion (CRVO). All 42 eyes received intravitreous injection of 40 mg/ml TA (0.1 ml) and then were followed up for 11-23.5 months. Eyes were examined by slit-lamp microscope, fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) and best corrected visual acuity (BCVA), and intraocular pressure (IOP) of those eyes were detected before and after treatment. Average vision of eyes was 0.1 before treatment, and the vision improved in one month (vision ≥ 0.2: 100%; vision ≥ 0.5: 42.9%) and three months (vision ≥ 0.2: 64.3%; vision ≥ 0.5: 21.4%) after treatment; but as time went on, the vision of some patients declined; at the last follow-up, patients with vision ≥ 0.2 accounted for 28.6% and those with vision ≥0.5 accounted for 7.1%; compared to before treatment, 71.4% patients had improved vision and the remaining 28.6% had declined vision. Some patients were observed with high IOP during treatment, and 7 eyes were found with secondary cataract in posterior capsule of lens at the last follow-up. Intravitreous injection of triamcinolone acetonide proved to have significant short-term curative effect on CEM which is non-sensitive to conventional therapies, but it is likely to induce high IPO and posterior capsular opacification.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Female ; Fluorescein Angiography ; Humans ; Macular Edema/diagnostic imaging ; Macular Edema/drug therapy ; Macular Edema/physiopathology ; Male ; Middle Aged ; Radiography ; Tomography, Optical Coherence ; Triamcinolone Acetonide/administration & dosage ; Triamcinolone Acetonide/adverse effects ; Triamcinolone Acetonide/therapeutic use ; Vision, Ocular ; Young Adult
Chemical Substances	Triamcinolone Acetonide (F446C597KA)
Language	English
Publishing date	2016-01
Publishing country	Italy
Document type	Clinical Trial ; Journal Article
ZDB-ID	639196-5
ISSN	1724-6083 ; 0393-974X
ISSN (online)	1724-6083
ISSN	0393-974X
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Article: [Effect of interleukin-7 on the anti-tumor function of CD8(+) T cells in patients with non-small cell lung cancer].

Zhang, X H / Wang, H M / Jin, L / Chang, R / Zu, F C / Li, F Z / Ku, C Y

Zhonghua yi xue za zhi

2018 Volume 98, Issue 30, Page(s) 2429–2433

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	CD8-Positive T-Lymphocytes ; Carcinoma, Non-Small-Cell Lung ; Cells, Cultured ; Humans ; Interferon-gamma ; Interleukin-2 ; Interleukin-7 ; Leukocytes, Mononuclear ; Lung Neoplasms ; Phosphorylation ; Signal Transduction ; Tumor Necrosis Factor-alpha
Chemical Substances	IL7 protein, human ; Interleukin-2 ; Interleukin-7 ; Tumor Necrosis Factor-alpha ; Interferon-gamma (82115-62-6)
Language	Chinese
Publishing date	2018-08-20
Publishing country	China
Document type	Journal Article
ZDB-ID	132513-9
ISSN	0376-2491
ISSN	0376-2491
DOI	10.3760/cma.j.issn.0376-2491.2018.30.012
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