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Article ; Online: Modified Minimally Invasive Bunnell Suture Surgery with Channel-assisted Minimally Invasive Reconstruction Device for Treating Achilles Tendon Rupture.

Li, Chao / Li, Fu-Chun

Zeitschrift fur Orthopadie und Unfallchirurgie

2024

Abstract: The aim of this study was to improve the process of microincision and endoscopic surgery for the treatment of Achilles tendon (AT) rupture using the modified minimally invasive Bunnell suture (MIBS) technique.From December 2019 to December 2021, 20 ... ...

Title translation	Modifizierte minimal-invasive Bunnell-Nahtoperation mit kanalunterstütztem minimal-invasivem Rekonstruktionsinstrument zur Behandlung der Achillessehnenruptur.
Abstract	The aim of this study was to improve the process of microincision and endoscopic surgery for the treatment of Achilles tendon (AT) rupture using the modified minimally invasive Bunnell suture (MIBS) technique.From December 2019 to December 2021, 20 patients with AT rupture who visited the First Affiliated Hospital of Harbin Medical University (Harbin, China) underwent MIBS surgery.A total of 20 patients were included, of whom 18 (90.0%) were male and 2 (10.0%) were female. The mean age of the patients was 37.75 ± 9.94 years. In terms of the site of the AT, two (10.0%) had surgery on their left AT. The mean duration of surgery was 23.00 ± 2.47 minutes, and the mean bleeding volume was 5.00 ± 1.12 mL. All 20 patients (100%) showed complete incisional healing (stage I healing) and normal functional recovery (3-6 months postoperatively). There were no cases of abnormal functional recovery or postoperative recurrence within the 3-6 month follow-up period. The Achilles tendon Total Rupture Score (ATRS) significantly improved post-surgery (83.6 ± 5.59) compared to pretreatment (0.3 ± 0.92, p < 0.0001), indicating successful patient recovery.After the modified MIBS surgical process, the operation steps were simplified, the surgical difficulty was reduced, and the surgical trauma was alleviated, resulting in good postoperative recovery and patient satisfaction with the outcome. Therefore, the MIBS surgery has high promotability.
Language	English
Publishing date	2024-04-29
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2280747-0
ISSN	1864-6743 ; 1438-941X ; 1864-6697 ; 0044-3220
ISSN (online)	1864-6743 ; 1438-941X
ISSN	1864-6697 ; 0044-3220
DOI	10.1055/a-2294-1043
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Article ; Online: The Arf family GTPases: Regulation of vesicle biogenesis and beyond.

Li, Fu-Long / Guan, Kun-Liang

BioEssays : news and reviews in molecular, cellular and developmental biology

2023 Volume 45, Issue 6, Page(s) e2200214

Abstract: The Arf family proteins are best known for their roles in the vesicle biogenesis. However, they also play fundamental roles in a wide range of cellular regulation besides vesicular trafficking, such as modulation of lipid metabolic enzymes, cytoskeleton ... ...

Abstract	The Arf family proteins are best known for their roles in the vesicle biogenesis. However, they also play fundamental roles in a wide range of cellular regulation besides vesicular trafficking, such as modulation of lipid metabolic enzymes, cytoskeleton remodeling, ciliogenesis, lysosomal, and mitochondrial morphology and functions. Growing studies continue to expand the downstream effector landscape of Arf proteins, especially for the less-studied members, revealing new biological functions, such as amino acid sensing. Experiments with cutting-edge technologies and in vivo functional studies in the last decade help to provide a more comprehensive view of Arf family functions. In this review, we summarize the cellular functions that are regulated by at least two different Arf members with an emphasis on those beyond vesicle biogenesis.
MeSH term(s)	ADP-Ribosylation Factors/metabolism
Chemical Substances	ADP-Ribosylation Factors (EC 3.6.5.2)
Language	English
Publishing date	2023-03-30
Publishing country	United States
Document type	Review ; Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	50140-2
ISSN	1521-1878 ; 0265-9247
ISSN (online)	1521-1878
ISSN	0265-9247
DOI	10.1002/bies.202200214
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Article: Modified Minimally Invasive Bunnell Suture Surgery with Channel-assisted Minimally Invasive Reconstruction Device for Treating Achilles Tendon Rupture

Li, Chao / Li, Fu-chun

Zeitschrift für Orthopädie und Unfallchirurgie

2024

Abstract: The aim of this study was to improve the process of microincision and endoscopic ... surgery for the treatment of Achilles tendon (AT) rupture using the modified ... minimally invasive Bunnell suture (MIBS) technique. From December 2019 to December 2021, ... ...

Abstract	The aim of this study was to improve the process of microincision and endoscopic surgery for the treatment of Achilles tendon (AT) rupture using the modified minimally invasive Bunnell suture (MIBS) technique. From December 2019 to December 2021, 20 patients with AT rupture who visited the First Affiliated Hospital of Harbin Medical University (Harbin, China) underwent MIBS surgery. A total of 20 patients were included, of whom 18 (90.0%) were male and 2 (10.0%) were female. The mean age of the patients was 37.75 ± 9.94 years. In terms of the site of the AT, two (10.0%) had surgery on their left AT. The mean duration of surgery was 23.00 ± 2.47 minutes, and the mean bleeding volume was 5.00 ± 1.12 mL. All 20 patients (100%) showed complete incisional healing (stage I healing) and normal functional recovery (3–6 months postoperatively). There were no cases of abnormal functional recovery or postoperative recurrence within the 3–6 month follow-up period. The Achilles tendon Total Rupture Score (ATRS) significantly improved post-surgery (83.6 ± 5.59) compared to pretreatment (0.3 ± 0.92, p < 0.0001), indicating successful patient recovery. After the modified MIBS surgical process, the operation steps were simplified, the surgical difficulty was reduced, and the surgical trauma was alleviated, resulting in good postoperative recovery and patient satisfaction with the outcome. Therefore, the MIBS surgery has high promotability.
Keywords	Achilles tendon rupture ; tunnel-assisted suture system ; minimally invasive surgery ; Riss der Achillessehne ; tunnelgestütztes Naht-System ; minimalinvasive Chirurgie
Language	English
Publishing date	2024-04-29
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2280747-0
ISSN	1864-6743 ; 1438-941X ; 1864-6697 ; 0044-3220
ISSN (online)	1864-6743 ; 1438-941X
ISSN	1864-6697 ; 0044-3220
DOI	10.1055/a-2294-1043
Database	Thieme publisher's database

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Article ; Online: Causal influences of osteoarthritis on COVID-19

Li Fu / Ancha Baranova / Hongbao Cao / Fuquan Zhang

Frontiers in Medicine, Vol

a Mendelian randomization study

2023 Volume 10

Abstract: ObjectiveAlthough observational and genetic studies have indicated a correlation between OA and COVID-19, it remains uncertain whether osteoarthritis (OA) contributes to the severity of COVID-19. Here, we aimed to investigate the potential causal links ... ...

Abstract	ObjectiveAlthough observational and genetic studies have indicated a correlation between OA and COVID-19, it remains uncertain whether osteoarthritis (OA) contributes to the severity of COVID-19. Here, we aimed to investigate the potential causal links between the two.MethodsIn this study, we conducted Mendelian randomization (MR) analysis to investigate whether there is a potential causal connection between OA and COVID-19 outcomes. The analysis utilized publicly available GWAS summary datasets, incorporating data on OA (N = 455,221), SARS-CoV-2 infection (N = 2,597,856), hospitalized COVID-19 (N = 2,095,324), and critical COVID-19 (N = 1,086,211). Additionally, we performed a literature analysis to establish a molecular network connecting OA and COVID-19.ResultsThe MR analysis showed causal effects of OA on hospitalized COVID-19 (OR: 1.21, 95% CI: 1.02–1.43, p = 0.026) and critical COVID-19 (OR: 1.35, 95% CI: 1.09–1.68, p = 0.006) but not on SARS-CoV-2 infection as such (OR: 1.00, 95% CI: 0.92–1.08, p = 0.969). Moreover, the literature-based pathway analysis uncovered a set of specific genes, such as CALCA, ACE, SIRT1, TNF, IL6, CCL2, and others, that were found to mediate the association between OA and COVID-19.ConclusionOur findings indicate that OA elevates the risk of severe COVID-19. Therefore, larger efforts should be made in the prevention of COVID-19 in OA patients.
Keywords	osteoarthritis ; COVID-19 ; Mendelian randomization ; GWAS ; ACE ; Medicine (General) ; R5-920
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The Role and Clinical Relevance of Osteopontin in Allergic Airway Diseases

Yang Liu / Li Fu / Zheng Liu

Journal of Clinical Medicine, Vol 12, Iss 2433, p

2023 Volume 2433

Abstract: The airway epithelium is exposed to numerous external irritants including infectious agents, environmental allergens, and atmospheric pollutants, releasing epithelial cytokines including thymic stromal lymphopoietin (TSLP), IL-33, and IL-25 and ... ...

Abstract	The airway epithelium is exposed to numerous external irritants including infectious agents, environmental allergens, and atmospheric pollutants, releasing epithelial cytokines including thymic stromal lymphopoietin (TSLP), IL-33, and IL-25 and initiating downstream type 2 (IL-4, IL-13, and IL-5) and IgE-driven pathways. These pathways trigger the initiation and progression of allergic airway diseases, including chronic rhinosinusitis with nasal polyps (CRSwNP), allergic rhinitis (AR), and allergic asthma. However, the use of biological agents that target downstream cytokines, such as IL-5, IL-4, and IL-13 receptors and IgE, might not be sufficient to manage some patients successfully. Instead of blocking downstream cytokines, targeting upstream epithelial cytokines has been proposed to address the complex immunologic networks associated with allergic airway diseases. Osteopontin (OPN), an extracellular matrix glyco-phosphoprotein, is a key mediator involved in Th1-related diseases, including systemic lupus erythematosus, multiple sclerosis, inflammatory bowel disease, and rheumatoid arthritis. Emerging evidence, including ours, indicates that epithelial-cell-derived OPN also plays an essential role in Th2-skewed airway diseases, including CRSwNP, AR, and allergic asthma involving the Th17 response. Therefore, we reviewed the current knowledge of epithelial-cell-derived OPN in the pathogenesis of three type-2-biased airway diseases and provided a direction for its future investigation and clinical relevance.
Keywords	osteopontin ; inflammation ; biomarker ; therapeutic target ; pathogenesis ; Medicine ; R
Subject code	610
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Prognostic analysis of uveal melanoma based on the characteristic genes of M2-type macrophages in the tumor microenvironment

Li Fu / Qun Huang / Yongfeng Wu / Diang Chen

BMC Bioinformatics, Vol 24, Iss 1, Pp 1-

2023 Volume 15

Abstract: Abstract Uveal melanoma arises from stromal melanocytes and is the most prevalent primary intraocular tumor in adults. It poses a significant diagnostic and therapeutic challenge due to its high malignancy and early onset of metastases. In recent years, ... ...

Abstract	Abstract Uveal melanoma arises from stromal melanocytes and is the most prevalent primary intraocular tumor in adults. It poses a significant diagnostic and therapeutic challenge due to its high malignancy and early onset of metastases. In recent years, there has been a growing interest in the role of diverse immune cells in tumor cell development and metastasis. Using The Cancer Genome Atlas and the gene expression omnibus databases, and the CIBERSORT method, we investigated the topography of intra-tumor immune infiltration in uveal melanoma in this research. We evaluated the prognosis of uveal melanoma patients using the M2 macrophage immune cell infiltration score in conjunction with clinical tumor patient data. We built a prognostic model based on the distinctive genes of M2 macrophages and combined it with patients’ clinical data in the database; we ran a survival prognostic analysis to authenticate the model’s accuracy. The functional study revealed the importance of macrophage-associated genes in the development of uveal melanoma. Moreover, the reliability of our prediction model was verified by combining tumor mutational load, immune checkpoint, and drug sensitivity, respectively. Our study provides a reference for the follow-up study of uveal melanoma.
Keywords	Uveal melanoma ; M2 macrophages ; Tumor microenvironment (TME) ; The Cancer Genome Atlas (TCGA) ; Gene expression omnibus (GEO) ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Biology (General) ; QH301-705.5
Subject code	610 ; 616
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: An effective treatment and suspicious adverse reaction to Ibrutinib in a patient diagnosed with splenic B-cell lymphoma/leukaemia with prominent nucleoli: A first case report.

Shen, Mei-Xiao / Li, Fu-Ling / Luo, Xian-Sheng / Wang, Zhi-Ming

Medicine

2024 Volume 102, Issue 52, Page(s) e36022

Abstract: Rationale: Splenic B-cell lymphoma/leukemia with prominent nucleoli (SBLPN) is a new classification, which is so rare that it lacks clinical data.: Patient concerns: An increased proportion of prolymphocytes (84%) in the bone marrow smear. Whole exon ...

Abstract	Rationale: Splenic B-cell lymphoma/leukemia with prominent nucleoli (SBLPN) is a new classification, which is so rare that it lacks clinical data. Patient concerns: An increased proportion of prolymphocytes (84%) in the bone marrow smear. Whole exon sequence analysis revealed a TP53 mutation. Diagnoses: Combining the clinical features with laboratory test results led to a diagnosis of SBLPN which was made according to the 5th edition of the WHO classification of hematolymphoid tumors, although the patient was diagnosed with B-PLL when guided by the 4th edition of the WHO classification. Interventions: The use of Ibrutinib as an effective treatment. Outcomes: The patient was in complete remission after 5 months of Ibrutinib and then died of sudden aortic dissection. Lessons: Ibrutinib was an effective regimen for SBLPN. Aortic dissection might be considered as a suspicious adverse reaction to Ibrutinib.
MeSH term(s)	Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/pathology ; Leukemia ; Lymphoma, B-Cell ; Aortic Dissection ; Adenine/analogs & derivatives ; Piperidines
Chemical Substances	ibrutinib (1X70OSD4VX) ; Adenine (JAC85A2161) ; Piperidines
Language	English
Publishing date	2024-01-11
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000036022
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Article ; Online: Total Three-Dimensional-Guided Laparoscopic Radical Resection for Left Perihilar Cholangiocarcinoma.

Ma, Wen-Jie / Yang, Chen / Jin, Yan-Wen / Li, Fu-Yu

Annals of surgical oncology

2024 Volume 31, Issue 5, Page(s) 3055–3056

Abstract: Background: The application of three-dimensional (3D) reconstruction has been extensively adopted in hepatectomy navigation,: Video: A 54-year-old male patient, classified as Child-Pugh B, presented a small neoplasm situated at the left hepatic duct ... ...

Abstract	Background: The application of three-dimensional (3D) reconstruction has been extensively adopted in hepatectomy navigation, Video: A 54-year-old male patient, classified as Child-Pugh B, presented a small neoplasm situated at the left hepatic duct proximate to the right hepatic and common hepatic ducts. An enhanced abdominal computed tomographic scan identified a solitary lesion measuring 2.8 × 2.4 cm. 3D reconstruction exposed tumor invasion into the left hepatic artery and left portal vein. Given the lesion's unique location, a pure laparoscopic left hepatectomy and caudate lobectomy were executed using a no-touch en block technique post patient consent. Concurrently, extrahepatic bile duct resection, radical lymphadenectomy with skeletonization, and biliary reconstruction were performed. Results: The 3D reconstruction-guided laparoscopic left hepatectomy and caudate lobectomy were successfully completed in 425 min with minimal blood loss (50 mL). The histological grading was T2bN0M0 (stage II). The patient was discharged on the sixth postoperative day without complications, and postoperative treatment included mono-drug chemotherapy with capecitabine. No recurrence was observed at the 6-month follow-up. Conclusion: Our experience suggests that 3D reconstruction-guided laparoscopic radical resection may offer increased precision and efficiency in selected pHCCA patients. This approach can potentially yield outcomes comparable with or superior to open surgery, given standardized lymph node dissection by skeletonization, use of the no-touch en block technique, appropriate digestive tract reconstruction, and reduced bleeding and liver damage.
MeSH term(s)	Male ; Humans ; Middle Aged ; Klatskin Tumor/surgery ; Klatskin Tumor/pathology ; Cholangiocarcinoma/surgery ; Bile Ducts, Intrahepatic/pathology ; Hepatectomy/methods ; Laparoscopy/methods ; Bile Duct Neoplasms/diagnostic imaging ; Bile Duct Neoplasms/surgery ; Bile Duct Neoplasms/pathology
Language	English
Publishing date	2024-01-19
Publishing country	United States
Document type	Case Reports ; Video-Audio Media ; Journal Article
ZDB-ID	1200469-8
ISSN	1534-4681 ; 1068-9265
ISSN (online)	1534-4681
ISSN	1068-9265
DOI	10.1245/s10434-024-14907-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: ASO Author Reflections: Total Three-Dimensional Guided Laparoscopic Radical Resection for Perihilar Cholangiocarcinoma-Transitioning from the Plane to the Realm of Dimensionality.

Ma, Wen-Jie / Zhou, Rong-Xing / Jin, Yan-Wen / Li, Fu-Yu

Annals of surgical oncology

2024 Volume 31, Issue 5, Page(s) 3102–3103

MeSH term(s)	Humans ; Klatskin Tumor/surgery ; Cholangiocarcinoma/surgery ; Bile Ducts, Intrahepatic ; Laparoscopy ; Bile Duct Neoplasms/surgery
Language	English
Publishing date	2024-01-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	1200469-8
ISSN	1534-4681 ; 1068-9265
ISSN (online)	1534-4681
ISSN	1068-9265
DOI	10.1245/s10434-024-14973-y
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Article: Reconstruction of Plantar Forefoot Area with Lateral Toe Pulp Flap: Case Report and Literature Review.

Hung, Chen-Ting / Lee, Jiunn-Tat / Wu, Meng-Si / Cheng, Li-Fu

Plastic and reconstructive surgery. Global open

2024 Volume 12, Issue 3, Page(s) e5654

Abstract: Reconstruction of the plantar forefoot area is challenging because it performs important functions, including carrying the body weight and balancing the ambulation gait, and lacks similar skin and soft tissues to manage the adjacent region. Herein, we ... ...

Abstract	Reconstruction of the plantar forefoot area is challenging because it performs important functions, including carrying the body weight and balancing the ambulation gait, and lacks similar skin and soft tissues to manage the adjacent region. Herein, we shared our experience of using a lateral toe pulp flap and reviewed the relevant literature on this topic. A 33-year-old man presented with a large granuloma in the left plantar forefoot area after undergoing multiple operations owing to the diagnosis of callus. After tumor excision, the wound exhibited tendon exposure and a large infected dead space in the myofascial layer. After serial debridement with negative pressure wound therapy, the wound, which measured ~3.5 × 2.5 cm
Language	English
Publishing date	2024-03-20
Publishing country	United States
Document type	Case Reports
ZDB-ID	2851682-5
ISSN	2169-7574 ; 2169-7574
ISSN (online)	2169-7574
ISSN	2169-7574
DOI	10.1097/GOX.0000000000005654
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