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Article ; Online: Role of ARRDC3 in enterovirus D68-infected A549 cells

LI Huayi / FU Huichao / SI Junzhuo / YANG Chun

陆军军医大学学报, Vol 45, Iss 14, Pp 1539-

2023 Volume 1546

Abstract: Objective To explore the expression of a-arrestin domain containing protein 3 (ARRDC3) in EV-D68 infected A549 cells and its effect on the biological behaviors of the cells. Methods The samples before and after EV-D68 infecting host cells (A549 cells) ... ...

Abstract	Objective To explore the expression of a-arrestin domain containing protein 3 (ARRDC3) in EV-D68 infected A549 cells and its effect on the biological behaviors of the cells. Methods The samples before and after EV-D68 infecting host cells (A549 cells) were analyzed by high-throughput whole transcriptome analysis with sequencing. After bioinformatics analysis was used to screen differentially expressed genes (DEGs), RT-qPCR and Western blotting were used to verify the expression of ARRDC3 at mRNA and protein levels. Then the A549 cells were designed and divided into 4 groups: control group (A549 cells not infected with EV-D68), infection group (A549 cells infected), knockdown ARRDC3 infection group (ARRDC3 siRNA interference-mediated knockdown in A549 cells after infection), knockdown control infection group (A549 cells infected with EV-D68 transfected with NC siRNA interference). Flow cytometry, CCK-8 assay, and Transwell assay were used to determine the effects of ARRDC3 on cell cycle, proliferation, and migration in above groups of cells. Results Gene sequencing showed there were 239 DEGs with more than 2-fold differential expression, with 135 up-regulated and 104 down-regulated genes, and ARRDC3 is one of DEGs. After A549 cells were infected with EV-D68, the expression level of ARRDC3 was significantly increased through RT-qPCR and Western blotting (P < 0.05), cell cycle was arrested and cell migration was inhibited. Silencing of ARRDC3 through siRNA transfection improved EV-D68 infection to A549 cells and prolonged the cell cycle arrested at DNA synthesis phase (P < 0.05). Although the silencing showed no significant effect on the proliferation of host cells, cell migration ability was obviously enhanced (P < 0.05). Conclusion ARRDC3 is significantly up-regulated after EV-D68 infects A549 cells, and exerts its influence on host cells by blocking the DNA synthesis phase and inhibiting migration of host cells.
Keywords	arrdc3 ; ev-d68 ; a549 cells ; cell cycle ; proliferation and migration ; Medicine (General) ; R5-920
Subject code	570
Language	Chinese
Publishing date	2023-07-01T00:00:00Z
Publisher	Editorial Office of Journal of Army Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Thesis ; Online: Nutrients translocation & plant growth in tissue culture

Li, Huayi

2020

Abstract: In conventional plant micropropagation exogenous sucrose is generally taken up by plants from the medium. Any treatment that improves sucrose assimilation or translocation might promote plant growth and micropropagation efficiency. Transpiration is ... ...

Abstract	In conventional plant micropropagation exogenous sucrose is generally taken up by plants from the medium. Any treatment that improves sucrose assimilation or translocation might promote plant growth and micropropagation efficiency. Transpiration is widely believed to be at a very low level in vitro and subsequently the water flow and nutrient translocation are supposed to be marginal. We investigated the relation between growth and sucrose translocation on the one hand and leaf transpiration on the other hand in vitro. It was found that both transpiration, including the transpiration roles of surface cuticle and of stomatal activity, and sucrose assimilation attributed by water transport contributed to biomass accumulation in plants as the main reason for growth. It was also examined the role of sucrose transporter genes in the process of exogenous sucrose translocation in roots. The results demonstrated the importance of SWEET11 and SWEET12 genes in exogenous sucrose assimilation and translocation in vitro. In addition we emphasized the contribution of aquaporin plasma membrane intrinsic proteins PIP1 to the growth of in vitro plants through investigating physiological characteristics in miRNA-induced-PIP1s-silenced plants. As a woody plant Malus domestica ‘Gala’ was used to test whether increased transpiration generally can lead to improved plantlet growth in shoot culture and to demonstrate the relationship between transpiration, stomatal and cuticular transpiration, and plantlet growth in vitro. A follow up of this research should focus on plantlet growth promotion by cuticle manipulation and sucrose assimilation and translocation via fresh cut section.
Keywords	Life Science
Subject code	580
Language	English
Publisher	Wageningen University
Publishing country	nl
Document type	Thesis ; Online
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Article ; Online: PD-1/PD-L1 immune checkpoint blockade in ovarian cancer: Dilemmas and opportunities.

Peng, Zikun / Li, Ming / Li, Huayi / Gao, Qinglei

Drug discovery today

2023 Volume 28, Issue 8, Page(s) 103666

Abstract: Immune checkpoint inhibitors (ICIs) have revolutionized treatment in oncology. Antibodies against PD-1/PD-L1 and ICI-based combinations are under clinical investigations in multiple cancers, including ovarian cancer. However, the success of ICIs has not ... ...

Abstract	Immune checkpoint inhibitors (ICIs) have revolutionized treatment in oncology. Antibodies against PD-1/PD-L1 and ICI-based combinations are under clinical investigations in multiple cancers, including ovarian cancer. However, the success of ICIs has not materialized in ovarian cancer, which remains one of the few malignancies where ICIs exhibit modest efficacy as either monotherapy or combination therapy. In this review, we summarize completed and ongoing clinical trials of PD-1/PD-L1 blockade in ovarian cancer, categorize the underlying mechanisms of resistance emergence, and introduce candidate approaches to rewire the tumor microenvironment (TME) to potentiate anti-PD-1/PD-L1 antibodies.
MeSH term(s)	Humans ; Female ; Immune Checkpoint Inhibitors/pharmacology ; Immune Checkpoint Inhibitors/therapeutic use ; B7-H1 Antigen ; Ovarian Neoplasms/drug therapy ; Antibodies ; Combined Modality Therapy ; Tumor Microenvironment
Chemical Substances	Immune Checkpoint Inhibitors ; B7-H1 Antigen ; Antibodies
Language	English
Publishing date	2023-06-09
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	1324988-5
ISSN	1878-5832 ; 1359-6446
ISSN (online)	1878-5832
ISSN	1359-6446
DOI	10.1016/j.drudis.2023.103666
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Article ; Online: Functional CRISPR screens in T cells reveal new opportunities for cancer immunotherapies.

Xiang, Minghua / Li, Huayi / Zhan, Yuanyuan / Ma, Ding / Gao, Qinglei / Fang, Yong

Molecular cancer

2024 Volume 23, Issue 1, Page(s) 73

Abstract: T cells are fundamental components in tumour immunity and cancer immunotherapies, which have made immense strides and revolutionized cancer treatment paradigm. However, recent studies delineate the predicament of T cell dysregulation in tumour ... ...

Abstract	T cells are fundamental components in tumour immunity and cancer immunotherapies, which have made immense strides and revolutionized cancer treatment paradigm. However, recent studies delineate the predicament of T cell dysregulation in tumour microenvironment and the compromised efficacy of cancer immunotherapies. CRISPR screens enable unbiased interrogation of gene function in T cells and have revealed functional determinators, genetic regulatory networks, and intercellular interactions in T cell life cycle, thereby providing opportunities to revamp cancer immunotherapies. In this review, we briefly described the central roles of T cells in successful cancer immunotherapies, comprehensively summarised the studies of CRISPR screens in T cells, elaborated resultant master genes that control T cell activation, proliferation, fate determination, effector function, and exhaustion, and highlighted genes (BATF, PRDM1, and TOX) and signalling cascades (JAK-STAT and NF-κB pathways) that extensively engage in multiple branches of T cell responses. In conclusion, this review bridged the gap between discovering element genes to a specific process of T cell activities and apprehending these genes in the global T cell life cycle, deepened the understanding of T cell biology in tumour immunity, and outlined CRISPR screens resources that might facilitate the development and implementation of cancer immunotherapies in the clinic.
MeSH term(s)	Humans ; T-Lymphocytes ; Clustered Regularly Interspaced Short Palindromic Repeats ; Immunotherapy ; Signal Transduction ; Neoplasms/genetics ; Neoplasms/therapy ; Tumor Microenvironment/genetics
Language	English
Publishing date	2024-04-05
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2091373-4
ISSN	1476-4598 ; 1476-4598
ISSN (online)	1476-4598
ISSN	1476-4598
DOI	10.1186/s12943-024-01987-z
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Article ; Online: Fixed-time sliding mode attitude control of a flexible spacecraft with rotating appendages connected by magnetic bearing.

Zhang, Gaowang / Wang, Feng / Chen, Jian / Li, Huayi

Mathematical biosciences and engineering : MBE

2021 Volume 19, Issue 3, Page(s) 2286–2309

Abstract: This study focuses on the attitude control of a flexible spacecraft comprising rotating appendages, magnetic bearings, and a satellite platform capable of carrying flexible solar panels. The kinematic and dynamic models of the spacecraft were established ...

Abstract	This study focuses on the attitude control of a flexible spacecraft comprising rotating appendages, magnetic bearings, and a satellite platform capable of carrying flexible solar panels. The kinematic and dynamic models of the spacecraft were established using Lagrange methods to describe the translation and rotation of the spacecraft system and its connected components. A simplified model of the dynamics of a five-degrees-of-freedom (DOF) active magnetic bearing was developed using the equivalent stiffness and damping methods based on the magnetic gap variations in the magnetic bearing. Next, a fixed-time sliding mode control method was proposed for each component of the spacecraft to adjust the magnetic gap of the active magnetic bearing, realize a stable rotation of the flexible solar panels, obtain a high inertia for the appendage of the spacecraft, and accurately control the attitude. Finally, the numerical simulation results of the proposed fixed-time control method were compared with those of the proportional-derivative control method to demonstrate the superiority and effectiveness of the proposed control law.
Language	English
Publishing date	2021-12-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2265126-3
ISSN	1551-0018 ; 1551-0018
ISSN (online)	1551-0018
ISSN	1551-0018
DOI	10.3934/mbe.2022106
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Article ; Online: Finite-time velocity-free relative position coordinated control of spacecraft formation with dynamic event triggered transmission.

Wu, Jiao / Qiu, Shi / Liu, Ming / Li, Huayi / Liu, Yuan

Mathematical biosciences and engineering : MBE

2022 Volume 19, Issue 7, Page(s) 6883–6906

Abstract: This paper investigates the finite-time relative position coordinated control problem of distributed spacecraft formation without velocity information over limited communication bandwidth. In this design, a dynamic event triggered transmission scheme ... ...

Abstract	This paper investigates the finite-time relative position coordinated control problem of distributed spacecraft formation without velocity information over limited communication bandwidth. In this design, a dynamic event triggered transmission scheme among spacecraft is designed to reduce communication burden, and a finite-time extended state observer is proposed to estimate the velocity information and the effects of non-linearity and disturbance of each spacecraft. A fast terminal sliding mode control law is developed to achieve finite-time coordination of the overall spacecraft formation. Finally, a numerical simulation is presented to demonstrate the effectiveness of the proposed control strategy.
Language	English
Publishing date	2022-06-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2265126-3
ISSN	1551-0018 ; 1551-0018
ISSN (online)	1551-0018
ISSN	1551-0018
DOI	10.3934/mbe.2022324
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Article: Targeting PI3K/AKT/mTOR Signaling Pathway in Breast Cancer.

Li, Huayi / Prever, Lorenzo / Hirsch, Emilio / Gulluni, Federico

Cancers

2021 Volume 13, Issue 14

Abstract: Breast cancer is the most frequently diagnosed cancer and the primary cause of cancer death in women worldwide. Although early diagnosis and cancer growth inhibition has significantly improved breast cancer survival rate over the years, there is a ... ...

Abstract	Breast cancer is the most frequently diagnosed cancer and the primary cause of cancer death in women worldwide. Although early diagnosis and cancer growth inhibition has significantly improved breast cancer survival rate over the years, there is a current need to develop more effective systemic treatments to prevent metastasis. One of the most commonly altered pathways driving breast cancer cell growth, survival, and motility is the PI3K/AKT/mTOR signaling cascade. In the past 30 years, a great surge of inhibitors targeting these key players has been developed at a rapid pace, leading to effective preclinical studies for cancer therapeutics. However, the central role of PI3K/AKT/mTOR signaling varies among diverse biological processes, suggesting the need for more specific and sophisticated strategies for their use in cancer therapy. In this review, we provide a perspective on the role of the PI3K signaling pathway and the most recently developed PI3K-targeting breast cancer therapies.
Language	English
Publishing date	2021-07-14
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers13143517
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Article ; Online: NiFe codoping-regulated amorphous/crystalline heterostructured Co-based hydroxides/tungstate with rich oxygen vacancies for efficient water oxidation catalysis.

Feng, Jiejie / Chu, Changshun / Liu, Jianting / Wei, Liling / Li, Huayi / Shen, Jianquan

Journal of colloid and interface science

2023 Volume 659, Page(s) 330–338

Abstract: Oxygen evolution reaction (OER) is a crucial half-reaction in water splitting, generating hydrogen for sustainable development, but it is often subject to sluggish kinetics. Abundant transition metal-based OER electrocatalysts have been utilized to ... ...

Abstract	Oxygen evolution reaction (OER) is a crucial half-reaction in water splitting, generating hydrogen for sustainable development, but it is often subject to sluggish kinetics. Abundant transition metal-based OER electrocatalysts have been utilized to expedite the process. However, traditional amorphous catalysts suffer from low conductivity, while the activity of crystalline catalysts is also unsatisfactory. Herein, an amorphous/crystalline heterostructured Co-based hydroxide/tungstate was meticulously constructed and further tailored using a NiFe codoping method (NiFeCoW). Following NiFe codoping, the electronic structure had been modulated, subsequently altering the adsorption toward intermediates. From the electrochemical measurements, the NiFeCoW catalyst demonstrated superior electrocatalytic activity for OER in alkaline media, with a minimal overpotential of 297 mV at 10 mA cm
Language	English
Publishing date	2023-12-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	241597-5
ISSN	1095-7103 ; 0021-9797
ISSN (online)	1095-7103
ISSN	0021-9797
DOI	10.1016/j.jcis.2023.12.133
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Book ; Online: Less Conservative Robust Reference Governors and Their Applications

Castroviejo-Fernandez, Miguel / Li, Huayi / Cotorruelo, Andrés / Garone, Emanuele / Kolmanovsky, Ilya

2024

Abstract: The applications of reference governors to systems with unmeasured set-bounded disturbances can lead to conservative solutions. This conservatism can be reduced by estimating the disturbance from output measurements and canceling it in the nominal ... ...

Abstract	The applications of reference governors to systems with unmeasured set-bounded disturbances can lead to conservative solutions. This conservatism can be reduced by estimating the disturbance from output measurements and canceling it in the nominal control law. In this paper, a reference governor based on such an approach is considered and time-varying, disturbance and state estimation errors bounding sets are derived. Consequently, the traditional implementation of a reference governor, which exploits a constraint admissible positively-invariant set of constant commands and initial states, is replaced by one which utilizes a time-dependent sequence of similar sets (which are not necessary nested). Examples are reported which include two applications to longitudinal control of aircraft that illustrate handling of elevator uncertainty and wing icing. Comment: 20 pages
Keywords	Electrical Engineering and Systems Science - Systems and Control
Subject code	005
Publishing date	2024-01-03
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A deep-learning-enabled diagnosis of ovarian cancer - Authors' reply.

Gao, Yue / Li, Huayi / Chen, Lingxi / Wu, Yuan / Ma, Ding / Gao, Qinglei

The Lancet. Digital health

2022 Volume 4, Issue 9, Page(s) e631

MeSH term(s)	Deep Learning ; Female ; Humans ; Ovarian Neoplasms
Language	English
Publishing date	2022-08-26
Publishing country	England
Document type	Letter ; Comment
ISSN	2589-7500
ISSN (online)	2589-7500
DOI	10.1016/S2589-7500(22)00145-5
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