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  1. Book ; Online ; E-Book: The SAGE Handbook of qualitative research design

    Flick, Uwe

    2022  

    Title variant Handbook of qualitative research
    Author's details editet by Uwe Flick
    Language English
    Size 1 Online-Ressource (xxxix, 1266 Seiten), Illustrationen
    Publisher SAGE Reference
    Publishing place Los Angeles ; London ; New Delhi ; Singapore ; Washington, DC ; Melbourne
    Document type Book ; Online ; E-Book
    Note Die Printversion erscheint in 2 Bänden
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT021308517
    ISBN 978-1-5297-6694-3 ; 9781526484321 ; 1-5297-6694-X ; 1526484323
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: MiR-509-3-5p inhibits colon cancer malignancy by suppressing GTSE1.

    Li, Ke

    Biochemical and biophysical research communications

    2021  Volume 570, Page(s) 175–183

    Abstract: Background: The overarching goal of this research was to identify the effect of miR-509-3-5p on colon cancer (CC) and its interaction with potential target gene GTSE1 in CC.: Methods: The miR-509-3-5p expression was ascertained after performing qRT- ... ...

    Abstract Background: The overarching goal of this research was to identify the effect of miR-509-3-5p on colon cancer (CC) and its interaction with potential target gene GTSE1 in CC.
    Methods: The miR-509-3-5p expression was ascertained after performing qRT-PCR analyses, and the ability of GTSE1 to influence this microRNA was detected after carrying out RNA pull-down assay. CCK-8 assay kit was first employed to determine the proliferation of the cells. To examine the migration and invasion level of HCT116 and SW480 cells, cell wound healing and transwell assay were later performed. After constructing luciferase reporter plasmids, luciferase reporter assay was used to confirm the impacts of miR-509-3-5p on GTSE1 in HCT116 and SW480 cells.
    Results: We found that miR-509-3-5p expression reduced in CC, and its overexpression inhibited the proliferation, migration and invasion of CC cells. We later discovered that miR-509-3-5p could target GTSE1 that was then proved to be an oncogene in CC.
    Conclusion: Our study uncovered that miR-509-3-5p regulated CC malignancy by suppressing target gene GTSE1.
    MeSH term(s) Base Sequence ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation/genetics ; Colonic Neoplasms/genetics ; Colonic Neoplasms/pathology ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Microtubule-Associated Proteins/genetics ; Microtubule-Associated Proteins/metabolism ; Neoplasm Invasiveness ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
    Chemical Substances GTSE1 protein, human ; MIRN509 microRNA, human ; MicroRNAs ; Microtubule-Associated Proteins ; RNA, Messenger
    Language English
    Publishing date 2021-07-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2021.07.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 116: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Activatable organic probes for in situ imaging of biomolecules.

    Li, Ke / Yang, Minghui

    Chemistry, an Asian journal

    2024  Volume 19, Issue 3, Page(s) e202301037

    Abstract: Biomolecules are fundamental for various chemical and biological processes of living organisms. High-resolution in situ imaging of the dynamics and local distribution of biomolecules may facilitate better interpretation of diverse complex cell events in ... ...

    Abstract Biomolecules are fundamental for various chemical and biological processes of living organisms. High-resolution in situ imaging of the dynamics and local distribution of biomolecules may facilitate better interpretation of diverse complex cell events in the biomedicine field. In different advanced imaging tools, fluorescence imaging-based activatable organic probes can be noninvasively and effortlessly internalized into cells and can be easily modified, which is essential for the in situ imaging of targets in living organisms. We here briefly summarize the existing general design strategies of activatable organic probes for retaining the fluorescence signal inside cells. We particularly describe the bioapplication of these probes for the in situ bioimaging. This review is expected to promote the development of new molecular tools for extending the application of these in situ imaging strategies to other biomolecules.
    MeSH term(s) Fluorescent Dyes/chemistry ; Optical Imaging/methods
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2024-01-02
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2233006-9
    ISSN 1861-471X ; 1861-4728
    ISSN (online) 1861-471X
    ISSN 1861-4728
    DOI 10.1002/asia.202301037
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Analogous Lubberts effect in photon counting detectors.

    Li, Ke

    Proceedings of SPIE--the International Society for Optical Engineering

    2020  Volume 11312

    Abstract: For a light burst generated by an x ray-scintillator interaction, the quasi-isotropic spreading of optical quanta determines that both the light collection efficiency and the spread function of the light burst depend on the depth ( ...

    Abstract For a light burst generated by an x ray-scintillator interaction, the quasi-isotropic spreading of optical quanta determines that both the light collection efficiency and the spread function of the light burst depend on the depth (
    Language English
    Publishing date 2020-03-16
    Publishing country United States
    Document type Journal Article
    ISSN 0277-786X
    ISSN 0277-786X
    DOI 10.1117/12.2549785
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Implantation of Micra AV: A leadless atrioventricular synchronous pacemaker.

    Li, Ke / Liu, Jinfeng

    Asian journal of surgery

    2023  Volume 46, Issue 12, Page(s) 5761–5762

    MeSH term(s) Humans ; Pacemaker, Artificial ; Atrial Fibrillation
    Language English
    Publishing date 2023-08-31
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 1068461-x
    ISSN 0219-3108 ; 1015-9584
    ISSN (online) 0219-3108
    ISSN 1015-9584
    DOI 10.1016/j.asjsur.2023.08.133
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2831: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  6. Article ; Online: Apigenin accelerates wound healing in diabetic mice by promoting macrophage M2-type polarization via increasing miR-21 expression.

    Li, Ke / Wu, Lijun / Jiang, Jingting

    Molecular and cellular biochemistry

    2024  

    Abstract: The alteration of inflammatory phenotype by macrophage polarization plays an important role in diabetic wound repair. Apigenin has been reported to be anti-inflammatory and promote tissue repair; however, whether it regulates macrophage polarization to ... ...

    Abstract The alteration of inflammatory phenotype by macrophage polarization plays an important role in diabetic wound repair. Apigenin has been reported to be anti-inflammatory and promote tissue repair; however, whether it regulates macrophage polarization to participate in diabetic wound repair remains to be investigated. We found that apigenin promoted miR-21 expression in LPS-stimulated RAW264.7 cells, inhibited cellular M1-type factor TNF-α and IL-1β secretion and increased M2-type factor IL-10 and TGF-β secretion, and accelerated macrophage conversion from M1 type to M2 type, whereas this protective effect of apigenin was counteracted by a miR-21 inhibitor. Moreover, we established a macrophage-HUVECs cell in vitro co-culture system and found that apigenin accelerated the migration, proliferation, and VEGF secretion of HUVECs by promoting macrophage miR-21 expression. Further, mechanistic studies revealed that this was mediated by the TLR4/Myd88/NF-κB axis. In in vivo study, diabetic mice had significantly delayed wound healing compared to non-diabetic mice, accelerated wound healing in apigenin-treated diabetic mice, and decreased M1-type macrophages and increased M2-type macrophages in wound tissues.
    Language English
    Publishing date 2024-01-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 184833-1
    ISSN 1573-4919 ; 0300-8177
    ISSN (online) 1573-4919
    ISSN 0300-8177
    DOI 10.1007/s11010-023-04885-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 892: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Rapid diagnosis of acute myocardial infarction through integrated microfluidic chips for detection of characteristic targets.

    Yuan, Yaling / Zhong, Yue / Yang, Yanbin / Li, Ke

    Analytical biochemistry

    2024  Volume 689, Page(s) 115502

    Abstract: Myoglobin (Myo), creatine kinase-MB (CKMB), and cardiac troponin I (cTnI) are crucial biomarkers for diagnosing acute myocardial infarction (AMI) The accurate and rapid detection of these three targets can greatly improve the prognosis of AMI patients. ... ...

    Abstract Myoglobin (Myo), creatine kinase-MB (CKMB), and cardiac troponin I (cTnI) are crucial biomarkers for diagnosing acute myocardial infarction (AMI) The accurate and rapid detection of these three targets can greatly improve the prognosis of AMI patients. Herein, this study developed a microfluidic immunofluorescence method that can detect all three targets in 10-15 min. Ultrasonic atomization and spray technology are used to modify the surface of the injection-molded microfluidic chip (MFC), which effectively solves the problem of biological cross-linking and antibody immobilization on the MFC surface. In addition, it improves the hydrophilicity of the chip surface, thus enhancing fluid self-driving effect. The linear response towards Myo, CKMB and cTnI range from 5 ng/mL to 500 ng/mL, 1 ng/mL to 70 ng/mL, and 0.05 ng/mL to 30 ng/mL, respectively. The intra-batch precision is ≤ 10%, and the inter-batch precision is ≤ 15%. Furthermore, this method shows good consistency compared with the BECKMAN ACCESS2 chemiluminescent immunoanalyzer. The present work provides an AMI diagnostic method with high sensitivity, good repeatability, high accuracy and simple operation, which can satisfy the needs of clinical diagnosis, and shows promising application prospects.
    MeSH term(s) Humans ; Microfluidics ; Myocardial Infarction/diagnosis ; Creatine Kinase, MB Form ; Prognosis ; Troponin I ; Biomarkers ; Myoglobin ; Sensitivity and Specificity
    Chemical Substances Creatine Kinase, MB Form (EC 2.7.3.2) ; Troponin I ; Biomarkers ; Myoglobin
    Language English
    Publishing date 2024-03-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1110-1
    ISSN 1096-0309 ; 0003-2697
    ISSN (online) 1096-0309
    ISSN 0003-2697
    DOI 10.1016/j.ab.2024.115502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 389: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article: An Effective Video Synopsis Approach with Seam Carving.

    Li, Ke / Yan, Bo / Wang, Weiyi / Gharavi, Hamid

    IEEE signal processing letters

    2024  Volume 23, Issue 1

    Abstract: With a growth of surveillance cameras, the amount of captured videos expands. Manually analyzing and retrieving surveillance video is labor intensive and expensive. It would be much more convenient to generate a video digest, with which we can view the ... ...

    Abstract With a growth of surveillance cameras, the amount of captured videos expands. Manually analyzing and retrieving surveillance video is labor intensive and expensive. It would be much more convenient to generate a video digest, with which we can view the video in a fast and motion-preserving way. In this paper, we propose a novel video synopsis approach to generate condensed video, which uses an object tracking method for extracting important objects. This method will generate video tubes and a seam carving method to condense the original video. Experimental results demonstrate that our proposed method can achieve a high condensation rate while preserving all the important objects of interest. Therefore, this approach can enable users to view the surveillance video with great efficiency.
    Language English
    Publishing date 2024-02-29
    Publishing country United States
    Document type Journal Article
    ISSN 1070-9908
    ISSN 1070-9908
    DOI 10.1109/lsp.2015.2496558
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Biotin-tagged fluorescent probe for in situ visualization of γ-glutamyl transpeptidase in cancerous cells and tissues.

    Li, Ke / Chen, Xu / Wang, Benhua / Liu, Sulai

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

    2024  Volume 315, Page(s) 124274

    Abstract: γ-Glutamyl transpeptidase (GGT), a cell-surface enzyme, is strongly implicated in mammalian malignancy growth and migration processes including human hepatocarcinogens. However, simply and conveniently detect of GGT on the cell membrane remains highly ... ...

    Abstract γ-Glutamyl transpeptidase (GGT), a cell-surface enzyme, is strongly implicated in mammalian malignancy growth and migration processes including human hepatocarcinogens. However, simply and conveniently detect of GGT on the cell membrane remains highly challenging. In this study, a biotin-tagged fluorescent probe Nap-biotin-glu was developed using glutamic acid, naphthalimide, and biotin as the reaction site, fluorescent reporter, and membrane-targeting group, which required only three steps. Colocalization fluorescence imaging and immunofluorescence analysis indicated that probe Nap-biotin-glu was successfully realized in situ visualizing of GGT on the cell membrane.Owing to the significant over-expressed GGT level in tumor, the probe was successfully applied to distinguish cancer tissues from adjacent normal tissues.
    Language English
    Publishing date 2024-04-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 210413-1
    ISSN 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
    ISSN (online) 1873-3557
    ISSN 0370-8322 ; 0584-8539 ; 1386-1425
    DOI 10.1016/j.saa.2024.124274
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 51/98: Show issues
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  10. Article ; Online: Knowledge, attitude, and practice of atrial fibrillation in high altitude areas.

    Li, Ke / Liu, Jinfeng / Zhu, Yan

    Frontiers in public health

    2024  Volume 12, Page(s) 1322366

    Abstract: Background: To investigate the knowledge, attitude, and practice (KAP) of atrial fibrillation (AF) among the general population in high-altitude areas.: Methodology: A web-based cross-sectional study was conducted among the general population in high- ...

    Abstract Background: To investigate the knowledge, attitude, and practice (KAP) of atrial fibrillation (AF) among the general population in high-altitude areas.
    Methodology: A web-based cross-sectional study was conducted among the general population in high-altitude areas.
    Results: A total of 786 valid questionnaires were enrolled, with a mean age of 34.75 ± 14.16 years. The mean score of knowledge, attitude and practice were 8.22 ± 6.50 (possible range: 0-10), 28.90 ± 5.63 (possible range: 8-40), 34.34 ± 6.44 (possible range: 9-45), respectively. The multivariate analysis showed that knowledge scores (OR = 1.108, 95% CI = 1.075-1.142,
    Conclusion: The general population in high-altitude regions had adequate knowledge, positive attitude, and proactive practice towards AF. The SEM was suitable for explaining general population' KAP regarding AF, revealing that knowledge directly and positively affected attitude and practice.
    MeSH term(s) Humans ; Atrial Fibrillation ; Female ; Male ; Cross-Sectional Studies ; Health Knowledge, Attitudes, Practice ; Altitude ; Adult ; Surveys and Questionnaires ; Middle Aged ; Young Adult ; Aged
    Language English
    Publishing date 2024-04-10
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2711781-9
    ISSN 2296-2565 ; 2296-2565
    ISSN (online) 2296-2565
    ISSN 2296-2565
    DOI 10.3389/fpubh.2024.1322366
    Database MEDical Literature Analysis and Retrieval System OnLINE

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