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Article ; Online: Nitratireductor luteus sp. nov. isolated from saline-alkali land

Yang, Xinping / Zhou, Liuyan / Wang, Xiaowu / Wang, Zhifang / Li, Pengbin / Dai, Jinping / Zhang, Huitao / Xie, Yuqing

Antonie van Leeuwenhoek. 2023 Mar., v. 116, no. 3 p.221-229

2023

Abstract: The Gram-staining negative, oxidase and catalase negative strain KC-ST17ᵀ, isolated from saline-alkali land, was characterized using a polyphasic approach to determine its taxonomic position. Using 16S rRNA gene sequence analysis, the highest similarity ... ...

Abstract	The Gram-staining negative, oxidase and catalase negative strain KC-ST17ᵀ, isolated from saline-alkali land, was characterized using a polyphasic approach to determine its taxonomic position. Using 16S rRNA gene sequence analysis, the highest similarity of strain KC-ST17ᵀ was found with Nitratireductor pacificus CCTCC AB 209302ᵀ (97.2%). Cells are aerobic, non-motile, and rod-shaped. The isolate was found to be able to grow in NaCl concentrations of 0–4.0%. The assembled genome of strain KC-ST17ᵀ had a total length of 4.9 Mb with a G + C content of 62.7%. According to genome analysis, strain KC-ST17ᵀ encodes genes involved in the reduction of nitrate to nitrite, which may play a role in the utilization of nitrogenous compounds from the soil as an immediate source of energy. Based on the phenotypic characteristics and phylogenetic analysis, strain KC-ST17ᵀ was confirmed to represent a novel species in the Nitratireductor genus; thus, the name Nitratireductor luteus sp. nov. was proposed. The type strain of this species was KC-ST17ᵀ (= KCTC 92119ᵀ = MCCC 1K07309ᵀ).
Keywords	Nitratireductor ; catalase ; energy ; genome ; nitrates ; nitrites ; nucleotide sequences ; phenotype ; phylogeny ; sequence analysis ; soil
Language	English
Dates of publication	2023-03
Size	p. 221-229.
Publishing place	Springer International Publishing
Document type	Article ; Online
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-022-01797-7
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Lysobacter chinensis sp. nov., a cellulose-degrading strain isolated from cow dung compost.

Liu, Yuyue / Zhou, Liuyan / Yang, Xinping / Li, Pengbin / Dai, Jinping / Xie, Yuqing / Wang, Xiaowu / Wang, Zhifang / Su, Zhanqiang / Feng, Lei

Antonie van Leeuwenhoek

2022 Volume 115, Issue 8, Page(s) 1031–1040

Abstract: A novel bacterial strain, TLK- ... ...

Abstract	A novel bacterial strain, TLK-CK17
MeSH term(s)	Animals ; Bacterial Typing Techniques ; Cattle ; Cellulose/metabolism ; Composting ; DNA, Bacterial/chemistry ; Fatty Acids/metabolism ; Lysobacter ; Nucleic Acid Hybridization ; Phospholipids/analysis ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/metabolism ; Sequence Analysis, DNA ; Soil Microbiology
Chemical Substances	DNA, Bacterial ; Fatty Acids ; Phospholipids ; RNA, Ribosomal, 16S ; Cellulose (9004-34-6)
Language	English
Publishing date	2022-06-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-022-01755-3
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Article ; Online: Nitratireductor luteus sp. nov. isolated from saline-alkali land.

Yang, Xinping / Zhou, Liuyan / Wang, Xiaowu / Wang, Zhifang / Li, Pengbin / Dai, Jinping / Zhang, Huitao / Xie, Yuqing

Antonie van Leeuwenhoek

2022 Volume 116, Issue 3, Page(s) 221–229

Abstract: The Gram-staining negative, oxidase and catalase negative strain KC- ... ...

Abstract	The Gram-staining negative, oxidase and catalase negative strain KC-ST17
MeSH term(s)	Fatty Acids/analysis ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; DNA, Bacterial/genetics ; Bacterial Typing Techniques ; Phospholipids/analysis
Chemical Substances	Fatty Acids ; RNA, Ribosomal, 16S ; DNA, Bacterial ; Phospholipids
Language	English
Publishing date	2022-12-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-022-01797-7
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Article: Lysobacter chinensis sp. nov., a cellulose-degrading strain isolated from cow dung compost

Liu, Yuyue / Zhou, Liuyan / Yang, Xinping / Li, Pengbin / Dai, Jinping / Xie, Yuqing / Wang, Xiaowu / Wang, Zhifang / Su, Zhanqiang / Feng, Lei

Antonie van Leeuwenhoek. 2022 Aug., v. 115, no. 8

2022

Abstract: A novel bacterial strain, TLK-CK17ᵀ, was isolated from cow dung compost sample. The strain was Gram-staining negative, non-gliding rods, aerobic, and displayed growth at 15–40 °C (optimally, 35 °C), with 0–5.0% (w/v) NaCl (optimally, 0.5) and at pH 6.5–8. ...

Abstract	A novel bacterial strain, TLK-CK17ᵀ, was isolated from cow dung compost sample. The strain was Gram-staining negative, non-gliding rods, aerobic, and displayed growth at 15–40 °C (optimally, 35 °C), with 0–5.0% (w/v) NaCl (optimally, 0.5) and at pH 6.5–8.5 (optimally, 7.0–7.5). The assembled genome of strain TLK-CK17ᵀ has a total length of 4.3 Mb with a G + C content of 68.2%. According to the genome analysis, strain TLK-CK17ᵀ encodes quite a few glycoside hydrolases that may play a role in the degradation of accumulated plant biomass in compost. On the basis 16S rRNA gene sequence analysis, strain TLK-CK17ᵀ showed the highest sequence similarity (98.9%) with L. penaei GDMCC 1.1817 ᵀ, followed by L. maris KCTC 42381 ᵀ (98.3%). Cells contained iso-C₁₆:₀, iso-C₁₅:₀, and summed feature 9 (comprising C₁₇:₁ ω9c and/or 10-methyl C₁₆:₀), as its major cellular fatty acids (> 10.0%) and ubiquinone-8 as the exclusively respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol prevailed among phospholipids. Based on the phenotypic, genomic and phylogenetic data, strain TLK-CK17ᵀ represents a novel species of the genus Lysobacter, for which the name Lysobacter chinensis sp. nov. is proposed, and the type strain is TLK-CK17ᵀ (= CCTCC AB2021257ᵀ = KCTC 92122 ᵀ).
Keywords	Lysobacter ; composts ; cow manure ; genome ; genomics ; glycosidases ; nucleotide sequences ; pH ; phenotype ; phosphatidylethanolamines ; phylogeny ; phytomass ; quinones ; sequence homology
Language	English
Dates of publication	2022-08
Size	p. 1031-1040.
Publishing place	Springer International Publishing
Document type	Article
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-022-01755-3
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Article ; Online: RBD class 1 and 2 antibody epitopes elicit around 70% neutralizing capacity against SARS-CoV-2 virus following boosting with inactivated virus vaccine.

Wang, Yuanyuan / Hu, Yunqi / Ma, Yong / Li, Pengbin / Zhou, Siwei / Xu, Mengxin / He, Bing / Liu, Shuning / Lv, Kexin / Liu, Sizhe / Zhang, Yu / Zhou, Na / Chen, Shifeng / Ye, Feng / Chen, Yao-Qing

Vaccine

2023 Volume 41, Issue 52, Page(s) 7641–7646

Abstract: A third dose of inactivated virus vaccine (IVV) boosts neutralizing antibodies, reducing SARS-CoV-2 transmission rate and COVID-19 severity. However, the impact of RBD-elicited antibodies and their neutralizing activity by the boost of IVV is unknown. We ...

Abstract	A third dose of inactivated virus vaccine (IVV) boosts neutralizing antibodies, reducing SARS-CoV-2 transmission rate and COVID-19 severity. However, the impact of RBD-elicited antibodies and their neutralizing activity by the boost of IVV is unknown. We investigated the impact of IVV's boost shot on RBD-elicited antibodies and their neutralizing activity in 18 subjects receiving the second and third IVV doses. Using an RBD antibodies depletion assay, we assessed the neutralizing activity of RBD-elicited antibodies. After the second dose, RBD-antigen elicitation accounted for ∼60% of neutralizing activity, which increased to 82% after the IVV boost against ancestral SARS-CoV-2. Depleting class 3 and class 4-specific antibodies with the Beta-RBD protein revealed that NAbs targeting RBD class 1 and class 2 subdomains increased from 57% to 75% post-boost. These findings highlight the significant enhancement of RBD-specific antibodies, especially against RBD class 1 and class 2, with IVV booster doses. Our study offers valuable insights for optimizing COVID-19 vaccine strategies.
MeSH term(s)	Humans ; SARS-CoV-2 ; Epitopes ; Vaccines, Inactivated ; COVID-19 Vaccines ; COVID-19/prevention & control ; Antibodies ; Antibodies, Blocking ; Antibodies, Neutralizing ; Antibodies, Viral
Chemical Substances	Epitopes ; Vaccines, Inactivated ; COVID-19 Vaccines ; Antibodies ; Antibodies, Blocking ; Antibodies, Neutralizing ; Antibodies, Viral
Language	English
Publishing date	2023-11-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.11.037
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Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Whole Exome Sequencing Facilitated the Identification of a Mosaic Small Supernumerary Marker Chromosome (sSMC).

Xing, Huan-Xia / Li, Peng-Bin / Cui, Li-Min / Jiang, Jian-Ye / Hu, Ning-Ning / Zhang, Xiao-Bin

BioMed research international

2021 Volume 2021, Page(s) 6258527

Abstract: Small supernumerary marker chromosomes (sSMCs) are a group of rare chromosomal anomalies, which pose challenges in the clinical practice of prenatal diagnosis and genetic counseling. This study enrolled an extended family with an underage male patient ... ...

Abstract	Small supernumerary marker chromosomes (sSMCs) are a group of rare chromosomal anomalies, which pose challenges in the clinical practice of prenatal diagnosis and genetic counseling. This study enrolled an extended family with an underage male patient displaying infantile seizures, intellectual disability, and retarded speech and psychomotor function. A series of multiplatform genetic detections was conducted to explore the diagnostic variation. Whole exome sequencing (WES) and chromosomal microarray analysis (CMA) indicated a mosaic sSMC derived from the pericentromeric region of chromosome 8 in the patient, which was confirmed using cytogenetic methods. The proband and his mother, who carried this mosaic variant, exhibited strong phenotypic variability. We also ruled out the pathogenicity of a
MeSH term(s)	Adult ; Base Sequence ; Centromere/genetics ; Child, Preschool ; Chromosomes, Human/genetics ; Cytogenetic Analysis ; Family ; Genetic Markers ; Humans ; Male ; Mosaicism ; Mutation/genetics ; Reproducibility of Results ; Whole Exome Sequencing
Chemical Substances	Genetic Markers
Language	English
Publishing date	2021-07-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2021/6258527
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Article ; Online: Gorlos-Phos for palladium-catalyzed borylation of aryl chlorides.

Li, Pengbin / Fu, Chunling / Ma, Shengming

Organic & biomolecular chemistry

2014 Volume 12, Issue 22, Page(s) 3604–3610

Abstract: Using a readily available form of the mono-phosphine ligand, Gorlos-Phos·HBF4, Pd-catalyzed borylation of aryl chlorides afforded aryl boronates in high yields. A variety of functional groups are well compatible with this palladium catalyzed borylation ... ...

Abstract	Using a readily available form of the mono-phosphine ligand, Gorlos-Phos·HBF4, Pd-catalyzed borylation of aryl chlorides afforded aryl boronates in high yields. A variety of functional groups are well compatible with this palladium catalyzed borylation reaction.
Language	English
Publishing date	2014-06-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2097583-1
ISSN	1477-0539 ; 1477-0520
ISSN (online)	1477-0539
ISSN	1477-0520
DOI	10.1039/c4ob00293h
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Article ; Online: Palladium-catalysed construction of butafulvenes.

Huang, Xin / Chen, Bing-Zhi / Li, Pengbin / Ji, Ding-Wei / Liu, Jinxian / Zheng, Hao / Yang, Sa-Na / Hu, Yan-Cheng / Wan, Boshun / Hu, Xiang-Ping / Fu, Chunling / Huang, Yankai / Zheng, Jian / Chen, Qing-An / Ma, Shengming

Nature chemistry

2022 Volume 14, Issue 10, Page(s) 1185–1192

Abstract: Butafulvene is a constitutional isomer of benzene, comprising a cyclobutene skeleton bearing two exocyclic conjugated methylene units. As a result of the intrinsic high strain energy and anti-aromaticity, the preparation of butafulvene compounds has been ...

Abstract	Butafulvene is a constitutional isomer of benzene, comprising a cyclobutene skeleton bearing two exocyclic conjugated methylene units. As a result of the intrinsic high strain energy and anti-aromaticity, the preparation of butafulvene compounds has been a fundamental issue for the development of butafulvene chemistry. Here an efficient palladium-catalysed coupling protocol involving propargylic compounds has been developed, providing a solid and versatile strategy for the rapid assembly of symmetric butafulvene derivatives. Based on mechanistic studies, two complementary mechanisms, both involving palladium catalysis, have been confirmed. With the mechanism unveiled, the synthesis of non-symmetric butafulvenes has also been achieved. Advantages of this strategy include tolerance to a wide range of propargylic molecules, mild reaction conditions, simple catalytic systems and easy scalability. The synthetic potential of the products as platform molecules for cyclobutene derivatives has also been demonstrated.
MeSH term(s)	Benzene ; Catalysis ; Palladium/chemistry
Chemical Substances	Palladium (5TWQ1V240M) ; Benzene (J64922108F)
Language	English
Publishing date	2022-08-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2464596-5
ISSN	1755-4349 ; 1755-4330
ISSN (online)	1755-4349
ISSN	1755-4330
DOI	10.1038/s41557-022-01017-9
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Article ; Online: Pd-catalyzed Suzuki coupling reaction of chloroalkylidene-β-lactones with LB-Phos as the ligand.

Li, Pengbin / Lü, Bo / Fu, Chunling / Ma, Shengming

Organic & biomolecular chemistry

2013 Volume 11, Issue 1, Page(s) 98–109

Abstract: In this paper, LB-Phos·HBF(4) salt has been applied for the Pd-catalysed Suzuki coupling reactions of optically active (Z)-α-choroalkylidene-β-lactones. Aryl, vinyl, alkyl and heteroaromatic boronic acids may be coupled with optically active β-lactones ... ...

Abstract	In this paper, LB-Phos·HBF(4) salt has been applied for the Pd-catalysed Suzuki coupling reactions of optically active (Z)-α-choroalkylidene-β-lactones. Aryl, vinyl, alkyl and heteroaromatic boronic acids may be coupled with optically active β-lactones affording corresponding optically active products highly selectively and efficiently.
MeSH term(s)	Boronic Acids/chemistry ; Catalysis ; Lactones/chemical synthesis ; Lactones/chemistry ; Ligands ; Models, Molecular ; Molecular Structure ; Organometallic Compounds/chemistry ; Palladium/chemistry
Chemical Substances	Boronic Acids ; Lactones ; Ligands ; Organometallic Compounds ; Palladium (5TWQ1V240M)
Language	English
Publishing date	2013-01-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2097583-1
ISSN	1477-0539 ; 1477-0520
ISSN (online)	1477-0539
ISSN	1477-0520
DOI	10.1039/c2ob26365c
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Article ; Online: Analysis of factors influencing parents' willingness to accept the quadrivalent influenza vaccine for school-aged children in the Nanhai District, China.

Li, Pengbin / Qiu, Zongyao / Feng, Wanling / Zeng, Hong / Chen, Weiming / Ke, Zhipan / Chen, Weiju / Lv, Haiyun / Luo, Guohan / Huang, Ximing

Human vaccines & immunotherapeutics

2019 Volume 16, Issue 5, Page(s) 1078–1085

Abstract: Recently, China has attached great importance to promoting immunization, prompting the media, scholars, and public to focus on its coverage and efficacy. This study aimed to understand the factors influencing parental willingness to have their school- ... ...

Abstract	Recently, China has attached great importance to promoting immunization, prompting the media, scholars, and public to focus on its coverage and efficacy. This study aimed to understand the factors influencing parental willingness to have their school-aged children vaccinated with quadrivalent influenza vaccines (QIVs). A cross-sectional study through face-to-face interviews was conducted between September and December 2018. Forty-four kindergartens and primary and junior high schools were randomly selected via stratified three-stage cluster sampling. Of 4,430 participants, 24.6% reported having heard of QIV and 24.2% reported having previously received information on QIV. Of these, 42.8% expressed willingness to obtain the QIV for their children. A junior college degree (adjusted odds ratio [aOR] = 1.447; 95% confidence interval [95% CI]: 1.202-1.742), higher influenza knowledge level (medium level, aOR = 1.150, 95% CI, 1.006-1.314; high level, aOR = 1.332, 95% CI, 1.045-1.697), and previous influenza information (aOR = 2.241; 95% CI, 1.604-3.130) were positively correlated with vaccination willingness. In contrast, no previous QIV-related information (aOR = 0.490; 95% CI, 0.418-0.575), no perceived susceptibility of children to influenza (aOR = 0.576; 95% CI, 0.489-0.680), fear of side effects (aOR = 0.599; 95% CI, 0.488-0.735), concern that vaccines need to be carefully administered (aOR = 0.728; 95% CI, 0.593-0.894), and mistrust of new vaccines (aOR = 0.730; 95% CI, 0.628-0.849) were pivotal barriers hindering parents from having their children vaccinated. This study provides baseline information for future immunization programs and delivery, with the ultimate goal of increasing vaccine uptake and minimizing school-wide influenza outbreaks.
MeSH term(s)	Child ; China/epidemiology ; Cross-Sectional Studies ; Health Knowledge, Attitudes, Practice ; Humans ; Influenza Vaccines ; Influenza, Human/prevention & control ; Parents ; Schools ; Surveys and Questionnaires ; Vaccination
Chemical Substances	Influenza Vaccines
Language	English
Publishing date	2019-08-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2019.1644881
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