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Article ; Online: Simultaneous improvement of thermostability and maltotriose-forming ability of a fungal α-amylase for bread making by directed evolution.

Wang, Yu-Chuan / Ma, Jun-Wen / Liu, Hai-Jie / Jiang, Zheng-Qiang / Li, Yan-Xiao

International journal of biological macromolecules

2024 Volume 264, Issue Pt 1, Page(s) 130481

Abstract: For applications in food industries, a fungal α-amylase from Malbranchea cinnamomea was engineered by directed evolution. Through two rounds of screening, a mutant α-amylase (mMcAmyA) was obtained with higher optimal temperature (70 °C, 5 °C increase) ... ...

Abstract	For applications in food industries, a fungal α-amylase from Malbranchea cinnamomea was engineered by directed evolution. Through two rounds of screening, a mutant α-amylase (mMcAmyA) was obtained with higher optimal temperature (70 °C, 5 °C increase) and better hydrolysis properties (18.6 % maltotriose yield, 2.5-fold increase) compared to the wild-type α-amylase (McAmyA). Site-directed mutations revealed that Threonine (Thr) 226 Serine (Ser) substitution was the main reason for the property evolution of mMcAmyA. Through high cell density fermentation, the highest expression level of Thr226Ser was 3951 U/mL. Thr226Ser was further used for bread baking with a dosage of 1000 U/kg flour, resulting in a 17.8 % increase in specific volume and a 35.6 % decrease in hardness compared to the control. The results were a significant improvement on those of McAmyA. Moreover, the mutant showed better anti-staling properties compared to McAmyA, as indicated by the improved sensory evaluation after 4 days of storage at 4 and 25 °C. These findings provide insights into the structure-function relationship of fungal α-amylase and introduce a potential candidate for bread-making industry.
MeSH term(s)	alpha-Amylases/genetics ; alpha-Amylases/metabolism ; Bread ; Hydrolysis ; Trisaccharides
Chemical Substances	alpha-Amylases (EC 3.2.1.1) ; maltotriose (639K0T34IK) ; Trisaccharides
Language	English
Publishing date	2024-02-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.130481
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Article ; Online: Tamarind Xyloglucan Oligosaccharides Attenuate Metabolic Disorders via the Gut–Liver Axis in Mice with High-Fat-Diet-Induced Obesity

Zhu, Chun-Hua / Li, Yan-Xiao / Xu, Yun-Cong / Wang, Nan-Nan / Yan, Qiao-Juan / Jiang, Zheng-Qiang

Foods. 2023 Mar. 24, v. 12, no. 7

2023

Abstract: Functional oligosaccharides exert obesity-reducing effects by acting at various pathological sites responsible for the development of obesity. In this study, tamarind xyloglucan oligosaccharides (TXOS) were used to attenuate metabolic disorders via the ... ...

Abstract	Functional oligosaccharides exert obesity-reducing effects by acting at various pathological sites responsible for the development of obesity. In this study, tamarind xyloglucan oligosaccharides (TXOS) were used to attenuate metabolic disorders via the gut–liver axis in mice with high-fat-diet (HFD)-induced obesity, as determined through LC/MS-MS and 16S rRNA sequencing technology. A TXOS dose equivalent to 0.39 g/kg/day in humans restored the gut microbiota in obese mice, which was in part supported by the key microflora, particularly Bifidobacterium pseudolongum. Moreover, TXOS reduced the abundance of opportunistic pathogen species, such as Klebsiella variicola and Romboutsia ilealis. The bodyweight and weight gain of TXOS-treated (4.8 g/kg per day) mice began to decrease at the 14th week, decreasing by 12.8% and 23.3%, respectively. Sixteen fatty acids were identified as potential biomarkers in the liver, and B. pseudolongum and caprylic acid were found to tightly regulate each other. This was associated with reduced inflammation in the liver, circulation, and adipose tissue and protection from metabolic disorders. The findings of this study indicate that TXOS can significantly increase the gut microbiota diversity of obese mice and restore the HFD-induced dysbiosis of gut microbiota.
Keywords	Bifidobacterium pseudolongum ; Klebsiella variicola ; adipose tissue ; biomarkers ; dysbiosis ; high fat diet ; inflammation ; intestinal microorganisms ; liver ; obesity ; octanoic acid ; oligosaccharides ; opportunistic pathogens ; tamarinds ; weight gain ; xyloglucans
Language	English
Dates of publication	2023-0324
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12071382
Database	NAL-Catalogue (AGRICOLA)

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Article: One-Pot Three-Enzyme System for Production of a Novel Prebiotic Mannosyl-β-(1 → 4)-Fructose Using a d-Mannose Isomerase from Xanthomonas phaseoli

Li, Yan-xiao / Hua, Xiao-han / Yan, Qiao-juan / Jin, Yan / Jiang, Zheng-qiang

Journal of agricultural and food chemistry. 2022 Sept. 19, v. 70, no. 38

2022

Abstract: The present supply of prebiotics is entirely inadequate to meet their demand. To produce novel prebiotics, a d-mannose isomerase (XpMIaseA) from Xanthomonas phaseoli was first produced in Komagataella phaffii (Pichia pastoris). XpMIaseA shared the ... ...

Abstract	The present supply of prebiotics is entirely inadequate to meet their demand. To produce novel prebiotics, a d-mannose isomerase (XpMIaseA) from Xanthomonas phaseoli was first produced in Komagataella phaffii (Pichia pastoris). XpMIaseA shared the highest amino acid sequence identity (58.0%) with the enzyme from Marinomonas mediterranea. Efficient secretory production of XpMIaseA (282.0 U mL–¹) was achieved using high cell density fermentation. The optimal conditions of XpMIaseA were pH 7.5 and 55 °C. It showed a broad substrate specificity, which isomerized d-mannose, d-talose, mannobiose, epilactose, and mannotriose. XpMIaseA was employed to construct a one-pot three-enzyme system for the production of mannosyl-β-(1 → 4)-fructose (MF) using mannan (5%, w/v) as the substrate. The equilibrium yield of MF was 58.2%. In in vitro fermentations, MF significantly stimulated (≤3.2-fold) the growth of 12 among 15 tested Bifidobacterium and Lactobacillus strains compared with fructo-oligosaccharides. Thus, the novel d-mannose isomerase provides a one-pot bioconversion strategy for efficiently producing novel prebiotics.
Keywords	Bifidobacterium ; Komagataella pastoris ; Lactobacillus ; Marinomonas mediterranea ; Xanthomonas campestris pv. phaseoli ; amino acid sequences ; biotransformation ; fermentation ; food chemistry ; fructooligosaccharides ; isomerases ; isomerization ; mannose ; pH ; prebiotics ; sequence analysis ; substrate specificity
Language	English
Dates of publication	2022-0919
Size	p. 12117-12127.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.2c04649
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Article ; Online: One-Pot Three-Enzyme System for Production of a Novel Prebiotic Mannosyl-β-(1 → 4)-Fructose Using a d-Mannose Isomerase from

Li, Yan-Xiao / Hua, Xiao-Han / Yan, Qiao-Juan / Jin, Yan / Jiang, Zheng-Qiang

Journal of agricultural and food chemistry

2022 Volume 70, Issue 38, Page(s) 12117–12127

Abstract: The present supply of prebiotics is entirely inadequate to meet their demand. To produce novel prebiotics, a d-mannose isomerase ( ...

Abstract	The present supply of prebiotics is entirely inadequate to meet their demand. To produce novel prebiotics, a d-mannose isomerase (
MeSH term(s)	Aldose-Ketose Isomerases ; Fructose ; Mannans/metabolism ; Mannose/metabolism ; Oligosaccharides/chemistry ; Xanthomonas
Chemical Substances	Mannans ; Oligosaccharides ; Fructose (30237-26-4) ; Aldose-Ketose Isomerases (EC 5.3.1.-) ; mannose isomerase (EC 5.3.1.7) ; Mannose (PHA4727WTP)
Language	English
Publishing date	2022-09-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.2c04649
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Article: Tamarind Xyloglucan Oligosaccharides Attenuate Metabolic Disorders via the Gut-Liver Axis in Mice with High-Fat-Diet-Induced Obesity.

Zhu, Chun-Hua / Li, Yan-Xiao / Xu, Yun-Cong / Wang, Nan-Nan / Yan, Qiao-Juan / Jiang, Zheng-Qiang

Foods (Basel, Switzerland)

2023 Volume 12, Issue 7

Abstract	Functional oligosaccharides exert obesity-reducing effects by acting at various pathological sites responsible for the development of obesity. In this study, tamarind xyloglucan oligosaccharides (TXOS) were used to attenuate metabolic disorders via the gut-liver axis in mice with high-fat-diet (HFD)-induced obesity, as determined through LC/MS-MS and 16S rRNA sequencing technology. A TXOS dose equivalent to 0.39 g/kg/day in humans restored the gut microbiota in obese mice, which was in part supported by the key microflora, particularly
Language	English
Publishing date	2023-03-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12071382
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Article: A novel neutral thermophilic β-mannanase from Malbranchea cinnamomea for controllable production of partially hydrolyzed konjac powder

Li, Yan-xiao / Wang, Nan-nan / Yan, Qiao-juan / Hua, Xiao-han / Liu, Yu / Jiang, Zheng-qiang

Applied microbiology and biotechnology. 2022 Mar., v. 106, no. 5-6

2022

Abstract: Partially hydrolyzed konjac powder (PHKP) can be used to increase the daily intake of dietary fibers of consumers. To produce PHKP by enzymatic hydrolysis, a novel β-mannanase gene (McMan5B) from Malbranchea cinnamomea was expressed in Pichia pastoris. ... ...

Abstract	Partially hydrolyzed konjac powder (PHKP) can be used to increase the daily intake of dietary fibers of consumers. To produce PHKP by enzymatic hydrolysis, a novel β-mannanase gene (McMan5B) from Malbranchea cinnamomea was expressed in Pichia pastoris. It showed a low identity of less than 52% with other GH family 5 β-mannanases. Through high cell density fermentation, the highest β-mannanase activity of 42200 U mL⁻¹ was obtained. McMan5B showed the maximal activity at pH 7.5 and 75 °C, respectively. It exhibited excellent pH stability and thermostability. Due to the different residues (Phe214, Pro253, and His328) in catalytic groove and the change of β2-α2 loop, McMan5B showed unique hydrolysis property as compared to other β-mannanases. The enzyme was employed to hydrolyze konjac powder for controllable production of PHKP with a weight-average molecular weight of 22000 Da (average degree of polymerization 136). Furthermore, the influence of PHKP (1.0%−4.0%) on the qualities of steamed bread was evaluated. The steamed bread adding 3.0% PHKP had the maximum specific volume and the minimum hardness, which showed 11.0% increment and 25.4% decrement as compared to the control, respectively. Thus, a suitable β-mannanase for PHKP controllable production and a fiber supplement for steamed bread preparation were provided in this study. KEY POINTS: • A novel β-mannanase gene (McMan5B) was cloned from Malbranchea cinnamomea and expressed in Pichia pastoris at high level. • McMan5B hydrolyzed konjac powder to yield partially hydrolyzed konjac powder (PHKP) instead of manno-oligosaccharides. • PHKP showed more positive effect on the quality of steamed bread than many other dietary fibers including konjac powder.
Keywords	Amorphophallus ; Komagataella pastoris ; Malbranchea ; biotechnology ; breads ; enzymatic hydrolysis ; fermentation ; genes ; hardness ; hydrolysis ; microbiology ; molecular weight ; pH stability ; polymerization ; thermal stability
Language	English
Dates of publication	2022-03
Size	p. 1919-1932.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-022-11832-2
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Article ; Online: Protective effects of lotus plumule ethanol extracts on bleomycin-induced pulmonary fibrosis in mice.

Guo, Peng / Li, Bin / Liu, Meng-Meng / Li, Yan-Xiao / Weng, Gong-Yu / Gao, Ying

Drug and chemical toxicology

2021 Volume 45, Issue 3, Page(s) 1432–1441

Abstract: Pulmonary fibrosis (PF) is a progressive fibrosing disease, characterized by excessive accumulation of extracellular matrix (ECM) and remodeling of the lung architecture, which finally result in respiratory failure. Currently, there is no satisfactory ... ...

Abstract	Pulmonary fibrosis (PF) is a progressive fibrosing disease, characterized by excessive accumulation of extracellular matrix (ECM) and remodeling of the lung architecture, which finally result in respiratory failure. Currently, there is no satisfactory treatment for PF， therefore, the development of effective agents is urgently needed. Lotus plumule, the green embryo of
MeSH term(s)	Animals ; Bleomycin/toxicity ; Drugs, Chinese Herbal/pharmacology ; Ethanol/toxicity ; Lotus/metabolism ; Lung ; Mice ; Pulmonary Fibrosis/chemically induced ; Pulmonary Fibrosis/drug therapy ; Pulmonary Fibrosis/prevention & control ; Transforming Growth Factor beta1/genetics ; Transforming Growth Factor beta1/metabolism
Chemical Substances	Drugs, Chinese Herbal ; Transforming Growth Factor beta1 ; Bleomycin (11056-06-7) ; Ethanol (3K9958V90M)
Language	English
Publishing date	2021-11-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	548368-2
ISSN	1525-6014 ; 0148-0545
ISSN (online)	1525-6014
ISSN	0148-0545
DOI	10.1080/01480545.2021.1993670
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Article: [Study on Regulation Effects of Yiqiyangyin Formula on Advanced Lung Cancer Cachexia Mice].

Li, Bin / Li, Yan-xiao / Pan, Hong-ran

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials

2018 Volume 39, Issue 6, Page(s) 1389–1392

Abstract: Objective: To observe the effect of Yiqiyangyin formula on advanced lung cancer cachexia mice.: Methods: 60 C57 BL /6inbred mice( SPF) were randomly divided into normal control group, model group, Yiqiyangyin formula group,indomethacin group, and ... ...

Abstract	Objective: To observe the effect of Yiqiyangyin formula on advanced lung cancer cachexia mice. Methods: 60 C57 BL /6inbred mice( SPF) were randomly divided into normal control group, model group, Yiqiyangyin formula group,indomethacin group, and cisplatin group, with 12 mice in each group. Model group and normal control group were treated with the same amount of normal saline. Except for the normal control group, the mice of the other groups were built with Lewis lung cancer model. The changes of the level of body mass, feed consumption,serum cytokine,the expression of TNF-α and its receptor were observed. Results: After eight days of treatment, compared with cisplatin group, the levels of CEA,NSE,Pro GRP in serum,CD3+,CD4+,CD4+/ CD8+levels in serum peripheral blood and TNF-R1 expressions in tumor tissues of Yiqiyangyin formula group were increased( P < 0. 05),the contents of TNF-α and IL-6 and the expressions of TNF-α and TNF-R2 in tumor tissues of Yiqiyangyin formula group were significantly decreased( P <0. 05). Conclusion: Yiqiyangyin formula affects on advanced lung cancer cachexia, which may be related to the regulation of the immune and inflammatory cytokines expression.
MeSH term(s)	Animals ; Cachexia ; Cytokines ; Indomethacin ; Lung ; Lung Neoplasms ; Male ; Mice ; Mice, Inbred C57BL
Chemical Substances	Cytokines ; Indomethacin (XXE1CET956)
Language	Chinese
Publishing date	2018-10-02
Publishing country	China
Document type	Journal Article
ISSN	1001-4454
ISSN	1001-4454
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Article ; Online: A novel neutral thermophilic β-mannanase from Malbranchea cinnamomea for controllable production of partially hydrolyzed konjac powder.

Li, Yan-Xiao / Wang, Nan-Nan / Yan, Qiao-Juan / Hua, Xiao-Han / Liu, Yu / Jiang, Zheng-Qiang

Applied microbiology and biotechnology

2022 Volume 106, Issue 5-6, Page(s) 1919–1932

Abstract	Partially hydrolyzed konjac powder (PHKP) can be used to increase the daily intake of dietary fibers of consumers. To produce PHKP by enzymatic hydrolysis, a novel β-mannanase gene (McMan5B) from Malbranchea cinnamomea was expressed in Pichia pastoris. It showed a low identity of less than 52% with other GH family 5 β-mannanases. Through high cell density fermentation, the highest β-mannanase activity of 42200 U mL
MeSH term(s)	Amorphophallus/genetics ; Cloning, Molecular ; Hydrogen-Ion Concentration ; Mannans/chemistry ; Onygenales ; Pichia/genetics ; Powders ; beta-Mannosidase/chemistry ; beta-Mannosidase/genetics
Chemical Substances	Mannans ; Powders ; beta-Mannosidase (EC 3.2.1.25)
Language	English
Publishing date	2022-02-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-022-11832-2
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Article: High level expression of a xyloglucanase from Rhizomucor miehei in Pichia pastoris for production of xyloglucan oligosaccharides and its application in yoghurt

Wang, Nan-nan / Li, Yan-xiao / Miao, Miao / Zhu, Chun-hua / Yan, Qiao-juan / Jiang, Zheng-qiang

International journal of biological macromolecules. 2021 Nov. 01, v. 190

2021

Abstract: The xyloglucanase gene (RmXEG12A) from Rhizomucor miehei CAU432 was successfully expressed in Pichia pastoris. The highest xyloglucanase activity of 25,700 U mL⁻¹ was secreted using high cell density fermentation. RmXEG12A was optimally active at pH 7.0 ... ...

Abstract	The xyloglucanase gene (RmXEG12A) from Rhizomucor miehei CAU432 was successfully expressed in Pichia pastoris. The highest xyloglucanase activity of 25,700 U mL⁻¹ was secreted using high cell density fermentation. RmXEG12A was optimally active at pH 7.0 and 65 °C, respectively. The xyloglucanase exhibited the highest specific activity towards xyloglucan (7915.5 U mg⁻¹). RmXEG12A was subjected to hydrolyze tamarind powder to produce xyloglucan oligosaccharides with the degree of polymerization (DP) 7–9. The hydrolysis ratio of xyloglucan in tamarind powder was 89.8%. Moreover, xyloglucan oligosaccharides (2.0%, w/w) improved the water holding capacity (WHC) of yoghurt by 1.1-fold and promoted the growth of Lactobacillus bulgaricus and Streptococcus thermophiles by 2.3 and 1.6-fold, respectively. Therefore, a suitable xyloglucanase for tamarind powder hydrolysis was expressed in P. pastoris at high level and xyloglucan oligosaccharides improved the quality of yoghurt.
Keywords	Komagataella pastoris ; Lactobacillus delbrueckii subsp. bulgaricus ; Rhizomucor miehei ; Streptococcus thermophilus ; fermentation ; genes ; hydrolysis ; oligosaccharides ; pH ; polymerization ; tamarinds ; xyloglucan-specific endo-beta-1,4-glucanase ; xyloglucans ; yogurt
Language	English
Dates of publication	2021-1101
Size	p. 845-852.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2021.09.035
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