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  1. Article: Application of thermosensitive-hydrogel combined with dental pulp stem cells on the injured fallopian tube mucosa in an animal model.

    Luo, Lihua / Zhu, Qunyan / Li, Yejian / Hu, Fengting / Yu, Jiangtao / Liao, Xiangyan / Xing, Zhenjie / He, Yan / Ye, Qingsong

    Frontiers in bioengineering and biotechnology

    2023  Volume 10, Page(s) 1062646

    Abstract: Objectives: ...

    Abstract Objectives:
    Language English
    Publishing date 2023-01-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2022.1062646
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Photosensitive Hydrogels Encapsulating DPSCs and AgNPs for Dental Pulp Regeneration.

    He, Yan / Zhang, Yanni / Hu, Fengting / Chen, Min / Wang, Ben / Li, Yejian / Xu, Haichao / Dong, Na / Zhang, Chen / Hu, Yunfan / Lin, Zhiqiang / Peng, Youjian / Ye, Qingsong / Luo, Lihua

    International dental journal

    2024  

    Abstract: Objective: Pulp regeneration with bioactive dentin-pulp complex has been a research hotspot in recent years. Stem cell therapy provided an interest strategy to regenerate the dental-pulp complex. Hence, this study aimed to evaluate the effects of ... ...

    Abstract Objective: Pulp regeneration with bioactive dentin-pulp complex has been a research hotspot in recent years. Stem cell therapy provided an interest strategy to regenerate the dental-pulp complex. Hence, this study aimed to evaluate the effects of photosensitive gelatin methacrylate (GelMA) hydrogel encapsulating dental pulp stem cells (DPSCs) and silver nanoparticles (AgNPs) for dental pulp regeneration in vitro.
    Methods: First, the AgNPs@GelMA hydrogels were prepared by lithium phenyl-2,4,6-trimethyl-benzoyl phosphinate (LAP) initiation via blue-light emitting diode light. The physical and chemical properties of AgNPs@GelMA hydrogels were comprehensively analysed via scanning electron microscopy (SEM), and mechanical characterisation, such as swelling ability, degradation properties, and AgNP release profile. Then, AgNPs@GelMA hydrogels encapsulated DPSCs were used to establish an AgNPs@GelMA biomimetic complex, further analysing its biocompatibility, antibacterial properties, and angiogenic capacity in vitro.
    Results: The results indicated that GelMA hydrogels demontrated optimal characteristics with a monomer:LAP ratio of 16:1. The physico-chemical properties of AgNPs@GelMA hydrogels did not change significantly after loading with AgNPs. There was no significant difference in AgNP release rate amongst different concentrations of AgNPs@GelMA hydrogels. Fifty to 200 μg/mL AgNPs@GelMA hydrogels could disperse E faecalis biofilm and reduce its metabolic activity . Furthermore, cell proliferation was arrested in 100 and 200 μg/mL AgNPs@GelMA hydrogels. The inhibition of 50 μg/mL AgNPs@GelMA hydrogels on E faecalis biofilm was above 50%, and the cell viability of the hydrogels was higher than 90%. The angiogenesis assay indicated that AgNPs@GelMA hydrogels encapsulating DPSCs could induce the formation of capillary-like structures and express angiogenic markers CD31, vascular endothelial growth factor , and von willebrand factor (vWF) in vitro.
    Conclusions: Results of this study indicate that 50 μg/mL AgNPs@GelMA hydrogels encapsulating DPSCs had significant antibacterial properties and angiogenic capacity, which could provide a significant experimental basis for the regeneration of the dentin-pulp complex.
    Language English
    Publishing date 2024-02-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 218262-2
    ISSN 1875-595X ; 0020-6539
    ISSN (online) 1875-595X
    ISSN 0020-6539
    DOI 10.1016/j.identj.2024.01.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 205: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

    Luo, Lihua / Xing, Zhenjie / Liao, Xiangyan / Li, Yejian / Luo, Yu / Ai, Yilong / He, Yan / Ye, Qingsong

    Cell proliferation

    2022  Volume 55, Issue 10, Page(s) e13293

    Abstract: Objectives: As a result of the current limitation of therapeutic strategies, the repair and regeneration of oviduct injuries required an alternative treatment. We present a novel approach to treat oviduct injuries through a dental pulp stem cells (DPSCs) ...

    Abstract Objectives: As a result of the current limitation of therapeutic strategies, the repair and regeneration of oviduct injuries required an alternative treatment. We present a novel approach to treat oviduct injuries through a dental pulp stem cells (DPSCs)-based therapy.
    Materials and methods: In vitro and in vivo models have been established. Immunofluorescence staining, flow cytometry and enzyme-linked immunosorbent assay (ELISA) analysis were used to investigate the features and angiogenic properties of DPSCs, as well as their impact on macrophages, in vitro. For the in vivo experiment with female SD rat model, immunohistochemical staining and ELISA analysis were used to assess the effects of DPSCs on the repair and regeneration of damaged oviducts.
    Results: The present data showed that intraperitoneal injection of DPSCs reduced the expression of IL-6 and TNF-α to inhibit the immunoreaction in injured sites, as well as increased the expression of VEGF to promote the in situ formation of vessel-like structures, thus the repair and recovery process could be initiated.
    Conclusions: We concluded that DPSCs-based therapy could be a novel potential technique for restoring the structure and function of damaged oviduct by enhancing immuno-regulated effect and promoting angiogenic property.
    MeSH term(s) Animals ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dental Pulp/metabolism ; Female ; Humans ; Immunomodulation ; Interleukin-6/metabolism ; Oviducts/metabolism ; Rats ; Rats, Sprague-Dawley ; Stem Cells ; Tumor Necrosis Factor-alpha/metabolism ; Vascular Endothelial Growth Factor A/metabolism
    Chemical Substances Interleukin-6 ; Tumor Necrosis Factor-alpha ; Vascular Endothelial Growth Factor A
    Language English
    Publishing date 2022-07-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 1064202-x
    ISSN 1365-2184 ; 0008-8730 ; 0960-7722
    ISSN (online) 1365-2184
    ISSN 0008-8730 ; 0960-7722
    DOI 10.1111/cpr.13293
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 532: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Influence of Fluoride-Resistant

    Zhang, Keke / Xiang, Yangfan / Peng, Youjian / Tang, Fengyu / Cao, Yanfan / Xing, Zhenjie / Li, Yejian / Liao, Xiangyan / Sun, Yan / He, Yan / Ye, Qingsong

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 801569

    Abstract: The widespread application of fluoride, an extremely effective caries prevention agent, induces the generation of fluoride-resistant strains of opportunistic cariogenic bacteria such as fluoride- ... ...

    Abstract The widespread application of fluoride, an extremely effective caries prevention agent, induces the generation of fluoride-resistant strains of opportunistic cariogenic bacteria such as fluoride-resistant
    MeSH term(s) Biofilms ; Cariostatic Agents ; Dental Caries/prevention & control ; Fluorides/pharmacology ; Humans ; In Situ Hybridization, Fluorescence ; Streptococcus mutans/genetics
    Chemical Substances Cariostatic Agents ; Fluorides (Q80VPU408O)
    Language English
    Publishing date 2022-02-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.801569
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Dental pulp stem cell-derived exosomes alleviate cerebral ischaemia-reperfusion injury through suppressing inflammatory response.

    Li, Song / Luo, Lihua / He, Yan / Li, Ruohan / Xiang, Yangfan / Xing, Zhenjie / Li, Yejian / Albashari, Abdullkhaleg Ali / Liao, Xiangyan / Zhang, Keke / Gao, Liang / Ye, Qingsong

    Cell proliferation

    2021  Volume 54, Issue 8, Page(s) e13093

    Abstract: Objectives: The study aimed to determine whether dental pulp stem cell-derived exosomes (DPSC-Exos) exert protective effects against cerebral ischaemia-reperfusion (I/R) injury and explore its underlying mechanism.: Materials and methods: Exosomes ... ...

    Abstract Objectives: The study aimed to determine whether dental pulp stem cell-derived exosomes (DPSC-Exos) exert protective effects against cerebral ischaemia-reperfusion (I/R) injury and explore its underlying mechanism.
    Materials and methods: Exosomes were isolated from the culture medium of human DPSC. Adult male C57BL/6 mice were subjected to 2 hours transient middle cerebral artery occlusion (tMCAO) injury followed by 2 hours reperfusion, after which singular injection of DPSC-Exos via tail vein was administrated. Brain oedema, cerebral infarction and neurological impairment were measured on day 7 after exosomes injection. Then, oxygen-glucose deprivation-reperfusion (OGD/R) induced BV2 cells were studied to analyse the therapeutic effects of DPSC-Exos on I/R injury in vitro. Protein levels of TLR4, MyD88, NF-κB p65, HMGB1, IL-6, IL-1β and TNF-α were determined by western blot or enzyme-linked immunosorbent assay. The cytoplasmic translocation of HMGB1 was detected by immunofluorescence staining.
    Results: DPSC-Exos alleviated brain oedema, cerebral infarction and neurological impairment in I/R mice. DPSC-Exos inhibited the I/R-mediated expression of TLR4, MyD88 and NF-κB significantly. DPSC-Exos also reduced the protein expression of IL-6, IL-1β and TNF-α compared with those of the control both in vitro and in vivo. Meanwhile, DPSC-Exos markedly decreased the HMGB1 cytoplasmic translocation induced by I/R damage.
    Conclusions: DPSC-Exos can ameliorate I/R-induced cerebral injury in mice. Its anti-inflammatory mechanism might be related with the inhibition of the HMGB1/TLR4/MyD88/NF-κB pathway.
    MeSH term(s) Animals ; Cell Survival ; Cytokines/metabolism ; Cytoplasm/metabolism ; Dental Pulp/cytology ; Dental Pulp/metabolism ; Disease Models, Animal ; Exosomes/metabolism ; Exosomes/transplantation ; HMGB1 Protein/metabolism ; Inflammation/metabolism ; Inflammation/therapy ; Interleukin-1beta/metabolism ; Interleukin-6/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Microglia/cytology ; Microglia/metabolism ; Myeloid Differentiation Factor 88/metabolism ; Reperfusion Injury/metabolism ; Reperfusion Injury/therapy ; Stem Cells/cytology ; Stem Cells/metabolism ; Toll-Like Receptor 4/metabolism ; Transcription Factor RelA/metabolism
    Chemical Substances Cytokines ; HMGB1 Protein ; Interleukin-1beta ; Interleukin-6 ; Myd88 protein, mouse ; Myeloid Differentiation Factor 88 ; Toll-Like Receptor 4 ; Transcription Factor RelA
    Language English
    Publishing date 2021-07-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 1064202-x
    ISSN 1365-2184 ; 0008-8730 ; 0960-7722
    ISSN (online) 1365-2184
    ISSN 0008-8730 ; 0960-7722
    DOI 10.1111/cpr.13093
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 532: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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