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  1. Article: A new automatic stitching method for full-length lower limb radiography.

    Alkhatatbeh, Tariq / Wang, Jia Lin / Zhang, Wei Jia / Li, Yong Wei / Xia, Yong / Wang, Wei

    Frontiers in surgery

    2022  Volume 9, Page(s) 1000074

    Abstract: Full-length lower limb x-rays are used to diagnose and plan surgical procedures, such as Total Knee Arthroplasty (TKA) and High Tibial Osteotomy (HTO). Due to the size limitation of digital radiography (DR), panoramic x-ray images cannot be obtained in a ...

    Abstract Full-length lower limb x-rays are used to diagnose and plan surgical procedures, such as Total Knee Arthroplasty (TKA) and High Tibial Osteotomy (HTO). Due to the size limitation of digital radiography (DR), panoramic x-ray images cannot be obtained in a single exposure, necessitating multiple exposures and image stitching. In favor of manually constructing full-length x-ray images, we propose a new feature-based automated method for stitching together x-ray images. This new method is based on Canny algorithm, which detects and aligns bone edges before fusing them using a Wavelet form domain. Twenty-eight sets of lower limb x-ray images obtained from our hospital have been stitched and evaluated. The hip, knee, and ankle (HKA) angle was computed in two different ways then compared to manually stitched x-ray images by an expert. The stitching time was only three seconds, and the
    Language English
    Publishing date 2022-10-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2773823-1
    ISSN 2296-875X
    ISSN 2296-875X
    DOI 10.3389/fsurg.2022.1000074
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Aberrant Expression of Cancer-Testis Antigen FBXO39 in Breast Cancer and its Clinical Significance.

    Liu, Yu-Chun / Yan, Sha / Liu, Dong-Mei / Pei, Dong-Xu / Li, Yong-Wei

    Clinical laboratory

    2020  Volume 66, Issue 9

    Abstract: Background: Cancer/testis antigen (CTA) is a class of antigen molecules expressed only in the germinal epithelium of testis and some tumor tissues. As an important CTA molecule, the expression of F-box protein 39 (FBXO39) in breast cancer (BC) and its ... ...

    Abstract Background: Cancer/testis antigen (CTA) is a class of antigen molecules expressed only in the germinal epithelium of testis and some tumor tissues. As an important CTA molecule, the expression of F-box protein 39 (FBXO39) in breast cancer (BC) and its clinical significance remain unclear. The objective of this study is to explore the value of FBXO39 in the diagnosis, efficacy monitoring, and prognostic evaluation of BC.
    Methods: The expression of FBXO39 mRNA in the serum exosomes of patients with BC before and after the initial diagnosis and treatment was detected by qRT-PCR, and the corresponding ROC curve was plotted. The expression of FBXO39 protein in BC cancer tissues was detected by immunohistochemistry, along with the analysis of the correlation between FBXO39 expression and clinical pathological features as well as prognosis of BC cases.
    Results: The serum-derived exosomes were successfully isolated and identified. The positive rate of FBXO39 mRNA in serum exosomes of patients with BC was up to 86%; there was a correlation between the expression level of serum exosomal FBXO39 and clinical staging, HER2, and Ki-67 expression (all with p < 0.05). The sensitivity of serum exosomal FBXO39 in distinguishing BC patients from healthy controls was 88%, with the specificity as 86%, and AUC as 0.9432. The expression change of FBXO39 in serum-sourced exosomes of patients with BC was related to their treatment situation, indicating that the level of FBXO39 decreased significantly after treatment. The expression of FBXO39 in cancer tissue was related to the clinical stage (p = 0.023) and lymphatic metastasis (p = 0.015) of the BC patients. Survival analysis showed that the expression of FBXO39 was negatively correlated with the prognosis of BC patients, with the high expression of FBXO39 indicating poor prognosis.
    Conclusions: Serum-derived exosomal FBXO39 could serve as an important indicator of BC diagnosis and efficacy evaluation; FBXO39 could be rated as an important indicator of BC prognosis evaluation.
    MeSH term(s) Biomarkers, Tumor/genetics ; Breast Neoplasms/diagnosis ; Breast Neoplasms/genetics ; Exosomes ; F-Box Proteins/genetics ; Humans ; Male ; Prognosis ; Testis
    Chemical Substances Biomarkers, Tumor ; F-Box Proteins ; FBXO39 protein, human
    Language English
    Publishing date 2020-10-08
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1307629-2
    ISSN 1433-6510 ; 0941-2131
    ISSN 1433-6510 ; 0941-2131
    DOI 10.7754/Clin.Lab.2020.200121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: [Effect of breastfeeding versus formula milk feeding on preterm infants in the neonatal intensive care unit].

    Li, Yong-Wei / Yan, Chao-Ying / Yang, Lei / Han, Zong-Lai

    Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

    2017  Volume 19, Issue 5, Page(s) 572–575

    Abstract: Objective: To investigate the importance of breastfeeding in preterm infants with various gestational ages.: Methods: A total of 639 preterm infants with a gestational age of 28: Results: Compared with the formula milk feeding group, the ... ...

    Abstract Objective: To investigate the importance of breastfeeding in preterm infants with various gestational ages.
    Methods: A total of 639 preterm infants with a gestational age of 28
    Results: Compared with the formula milk feeding group, the breastfeeding group had a significantly faster increase in body weight, a significantly lower incidence rate of NEC, a significantly higher ALP level, and a significantly lower Alb level in the preterm infants with a gestational age of 28-30 weeks (P<0.05); there were no significant differences between the two groups in the incidence rates of anemia, ROP, bronchopulmonary dysplasia (BPD), and nosocomial infection and length of hospital stay (P>0.05). For the preterm infants with a gestational age of 31-33 weeks, the breastfeeding group had a significantly faster increase in body weight, a significantly lower incidence rate of feeding intolerance, a significantly shorter length of hospital stay, and a significantly higher ALP level (P<0.05); there were no significant differences between the two groups in the incidence rates of NEC, anemia, ROP, BPD, and nosocomial infection and the Alb level (P>0.05). For the preterm infants with a gestational age of 34-36 weeks, there were no significant differences in these indices between the two groups (P>0.05).
    Conclusions: Breastfeeding plays an important role in increasing body weight, reducing the incidence rates of feeding intolerance and NEC, and shortening the length of hospital stay in preterm infants with a gestational age of 28-33 weeks.
    MeSH term(s) Breast Feeding ; Bronchopulmonary Dysplasia/etiology ; Enterocolitis, Necrotizing/etiology ; Humans ; Infant Formula ; Infant, Newborn ; Infant, Premature ; Intensive Care Units, Neonatal ; Retinopathy of Prematurity/etiology
    Language Chinese
    Publishing date 2017-05-10
    Publishing country China
    Document type Comparative Study ; Journal Article
    ISSN 1008-8830
    ISSN 1008-8830
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Feasibility study of a human papillomavirus E6 and E7 oncoprotein test for the diagnosis of cervical precancer and cancer.

    Zhang, Jin-Jun / Cao, Xin-Chun / Zheng, Xiang-Yu / Wang, Hai-Ying / Li, Yong-Wei

    The Journal of international medical research

    2018  Volume 46, Issue 3, Page(s) 1033–1042

    Abstract: Objective To evaluate the clinical value of human papillomavirus (HPV) E6 and E7 oncoprotein (HPV E6/E7) detection in the early screening of cervical cancer. Methods This prospective study evaluated all patients with suspected cervical intraepithelial ... ...

    Abstract Objective To evaluate the clinical value of human papillomavirus (HPV) E6 and E7 oncoprotein (HPV E6/E7) detection in the early screening of cervical cancer. Methods This prospective study evaluated all patients with suspected cervical intraepithelial neoplasia (CIN) as identified by the presence of at least one positive indicator from a ThinPrep cytologic test (TCT) and/or a Hybrid Capture 2 (HC2) HPV DNA test. The levels of E6/E7 oncoproteins were determined using Western blot analysis. The diagnostic value of the HPV E6/E7 protein assay was compared with the clinical diagnosis from TCT, HC2 and the gold standard of cervical biopsy histology. Results A total of 450 patients were enrolled in the study and based on histological findings, 102 patients were diagnosed with CIN1 (22.7%), 241 with CIN2 (53.6%), 96 with CIN3 (21.3%) and 11 with squamous cell carcinoma (2.4%). For a diagnosis of CIN2+, although the sensitivity of the HPV E6/E7 assay was lower than HC2 (65.5% versus 96.6%, respectively), the specificity was higher (38.2% versus 5.9%, respectively). The sensitivity of the HPV E6/E7 assay was higher than TCT (65.5% versus 36.2%, respectively). Conclusion Measuring HPV E6/E7 oncoprotein levels is a potential new biomarker for HPV type 16.
    MeSH term(s) Adult ; Biomarkers/metabolism ; Carcinoma, Squamous Cell/diagnosis ; Carcinoma, Squamous Cell/virology ; Cervical Intraepithelial Neoplasia/diagnosis ; Cervical Intraepithelial Neoplasia/virology ; DNA, Viral/genetics ; Diagnosis, Differential ; Feasibility Studies ; Female ; Histocytochemistry ; Human papillomavirus 16/genetics ; Human papillomavirus 16/isolation & purification ; Humans ; Middle Aged ; Oncogene Proteins, Viral/genetics ; Papillomavirus E7 Proteins/genetics ; Papillomavirus Infections/diagnosis ; Papillomavirus Infections/virology ; Prospective Studies ; Repressor Proteins/genetics ; Uterine Cervical Neoplasms/diagnosis ; Uterine Cervical Neoplasms/virology
    Chemical Substances Biomarkers ; DNA, Viral ; E6 protein, Human papillomavirus type 16 ; Oncogene Proteins, Viral ; Papillomavirus E7 Proteins ; Repressor Proteins ; oncogene protein E7, Human papillomavirus type 16
    Language English
    Publishing date 2018-01-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 184023-x
    ISSN 1473-2300 ; 0300-0605 ; 0142-2596
    ISSN (online) 1473-2300
    ISSN 0300-0605 ; 0142-2596
    DOI 10.1177/0300060517736913
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: [Value of abdominal ultrasound in the diagnosis of neonatal necrotizing enterocolitis and evaluation of disease severity].

    Yang, Lei / Xu, Wei / Li, Yong-Wei / Yan, Chao-Ying

    Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics

    2016  Volume 18, Issue 2, Page(s) 108–112

    Abstract: Objective: To investigate the value of abdominal ultrasound in diagnosing neonatal necrotizing enterocolitis (NEC) and its significance in evaluating the disease severity.: Methods: The clinical data of 84 neonates who were diagnosed with NEC between ...

    Abstract Objective: To investigate the value of abdominal ultrasound in diagnosing neonatal necrotizing enterocolitis (NEC) and its significance in evaluating the disease severity.
    Methods: The clinical data of 84 neonates who were diagnosed with NEC between July 2013 and January 2015 were analyzed retrospectively. According to the modified Bell-NEC staging criteria, these neonates were divided into a suspected NEC group (n=44) and a confirmed NEC group (n=40); according to clinical prognosis, they were divided into a medical treatment and full recovery group (n=58) and a surgery/death group (n=26). The changes in the results of abdominal ultrasound and abdominal X-ray plain film were compared between groups.
    Results: In the confirmed NEC group, abdominal ultrasound showed significantly higher detection rates of portal venous gas and dilatation of the intestine than abdominal X-ray plain film (P<0.05). Compared with the medical treatment and full recovery group, the surgery/death group had significantly higher detection rates of dilatation of intestine, bowel wall thickening, peritoneal effusion and free intraperitoneal air (P<0.05). Dilatation of the intestine and free intraperitoneal air shown by abdominal X-ray plain film were more common in the surgery/death group.
    Conclusions: Abdominal ultrasound is useful for the diagnosis of NEC. Ultrasonic findings can contribute to the prediction of the severity of NEC.
    MeSH term(s) Abdomen/diagnostic imaging ; Enterocolitis, Necrotizing/diagnosis ; Enterocolitis, Necrotizing/diagnostic imaging ; Female ; Humans ; Infant ; Infant, Newborn ; Infant, Newborn, Diseases/diagnosis ; Infant, Newborn, Diseases/diagnostic imaging ; Male ; Retrospective Studies ; Ultrasonography
    Language Chinese
    Publishing date 2016-02-15
    Publishing country China
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1008-8830
    ISSN 1008-8830
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Hepatocellular carcinoma and hepatitis B surface protein.

    Li, Yong-Wei / Yang, Feng-Cai / Lu, Hui-Qiong / Zhang, Jiong-Shan

    World journal of gastroenterology

    2016  Volume 22, Issue 6, Page(s) 1943–1952

    Abstract: The tumorigenesis of hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful for judging the natural disease ... ...

    Abstract The tumorigenesis of hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful for judging the natural disease course and guiding treatment. Truncated and mutated preS/S are produced by integrated viral sequences that are defective for replication. The preS/S mutants are considered "precursor lesions" of HCC. Different preS/S mutants induce various mechanisms of tumorigenesis, such as transactivation of transcription factors and an immune inflammatory response, thereby contributing to HCC. The preS2 mutants and type II "Ground Glass" hepatocytes represent novel biomarkers of HBV-associated HCC. The preS mutants may induce the unfolded protein response and endoplasmic reticulum stress-dependent and stress-independent pathways. Treatments to inhibit hepatitis B surface antigen (HBsAg) and damage secondary to HBsAg or the preS/S mutants include antivirals and antioxidants, such as silymarin, resveratrol, and glycyrrhizin acid. Methods for the prevention and treatment of HCC should be comprehensive.
    MeSH term(s) Animals ; Antioxidants/therapeutic use ; Antiviral Agents/therapeutic use ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/pathology ; Carcinoma, Hepatocellular/prevention & control ; Carcinoma, Hepatocellular/virology ; Cell Transformation, Viral ; Endoplasmic Reticulum Stress ; Genotype ; Hepatitis B/complications ; Hepatitis B/diagnosis ; Hepatitis B/drug therapy ; Hepatitis B/virology ; Hepatitis B Surface Antigens/genetics ; Hepatitis B Surface Antigens/immunology ; Hepatitis B Surface Antigens/metabolism ; Hepatitis B virus/drug effects ; Hepatitis B virus/genetics ; Hepatitis B virus/immunology ; Hepatitis B virus/metabolism ; Host-Pathogen Interactions ; Humans ; Liver Neoplasms/metabolism ; Liver Neoplasms/pathology ; Liver Neoplasms/prevention & control ; Liver Neoplasms/virology ; Mutation ; Risk Factors
    Chemical Substances Antioxidants ; Antiviral Agents ; Hepatitis B Surface Antigens
    Language English
    Publishing date 2016-02-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v22.i6.1943
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Silencing of Armadillo Repeat-Containing Protein 8 (ARMc8) Inhibits TGF-β-Induced EMT in Bladder Carcinoma UMUC3 Cells.

    Liang, Xuan / Men, Qun-Li / Li, Yong-Wei / Li, He-Cheng / Chong, Tie / Li, Zhao-Lun

    Oncology research

    2017  Volume 25, Issue 1, Page(s) 99–105

    Abstract: Armadillo repeat-containing protein 8 (ARMc8) is a key factor in regulating cell migration, proliferation, tissue maintenance, and tumorigenesis. However, its role in bladder cancer remains unknown. Thus, in this study we sought to investigate the effect ...

    Abstract Armadillo repeat-containing protein 8 (ARMc8) is a key factor in regulating cell migration, proliferation, tissue maintenance, and tumorigenesis. However, its role in bladder cancer remains unknown. Thus, in this study we sought to investigate the effect of ARMc8 on the epithelial-to-mesenchymal transition (EMT) progress in bladder cancer cells induced by transforming growth factor-β1 (TGF-β1). Our results found that ARMc8 was highly expressed in bladder cancer cell lines. ARMc8 silencing inhibited the TGF-β1-induced migration and invasion and suppressed the EMT progress in bladder cancer cells. Furthermore, ARMc8 silencing inhibited the TGF-β1-induced expression of β-catenin, cyclin D1, and c-myc in bladder cancer cells. In conclusion, the present study demonstrates a novel function for ARMc8, which acts as a mediator for TGF-β1-induced cell migration/invasion through modulation of the Wnt/β-catenin signaling pathway in bladder cancer cells. This study suggests that ARMc8 may be a potential therapeutic target for the development of therapies for bladder cancer.
    MeSH term(s) Armadillo Domain Proteins/genetics ; Cell Line, Tumor ; Cell Movement/drug effects ; Cell Movement/genetics ; Epithelial-Mesenchymal Transition/drug effects ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression ; Gene Silencing ; Humans ; RNA, Small Interfering/genetics ; Transforming Growth Factor beta/metabolism ; Transforming Growth Factor beta/pharmacology ; Urinary Bladder Neoplasms/genetics ; Urinary Bladder Neoplasms/metabolism ; Wnt Signaling Pathway/drug effects
    Chemical Substances ARMC8 protein, human ; Armadillo Domain Proteins ; RNA, Small Interfering ; Transforming Growth Factor beta
    Language English
    Publishing date 2017-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1114699-0
    ISSN 1555-3906 ; 0965-0407
    ISSN (online) 1555-3906
    ISSN 0965-0407
    DOI 10.3727/096504016X14719078133609
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Association between IL-6 Gene (-174 & -572 G/C) Polymorphisms and Endometrial Adenocarcinoma Risk.

    Wang, Hai-Ying / Zhang, Jin-Jun / Zheng, Xiang-Yu / Liu, Jian-Hua / Li, Yong-Wei

    Pathology oncology research : POR

    2016  Volume 22, Issue 4, Page(s) 825–829

    Abstract: We aimed to evaluate the association of IL-6 gene polymorphisms at positions of -174 and -572 and predisposition of endometrial adenocarcinoma (EAC) in a Chinese population. EAC patients have remarkably higher frequency of IL-6 -174 CC genotype [odds ... ...

    Abstract We aimed to evaluate the association of IL-6 gene polymorphisms at positions of -174 and -572 and predisposition of endometrial adenocarcinoma (EAC) in a Chinese population. EAC patients have remarkably higher frequency of IL-6 -174 CC genotype [odds ratio (OR) =1.56, 95 % confidence interval (CI) =1.07-2.23; P = 0.03], IL-6 -572 CC genotype (OR =1.93, 95%CI =1.17-3.15; P = 0.01) and IL-6 -174 C allele (OR =1.22, 95 % CI =1.03-1.46; P = 0.04) compared with healthy controls. When stratified with FIGO stage, patients with III-IV EAC have a significantly higher frequency of IL-6 -174 CC genotype (OR =1.66, 95% CI =1.06-2.58; P = 0.02) than healthy controls. The CC genotype of IL-6 gene polymorphisms at positions of -174 and -572 may denote potential high risk of EAC.
    MeSH term(s) Adenocarcinoma/genetics ; Alleles ; Asian Continental Ancestry Group/genetics ; Case-Control Studies ; Endometrial Neoplasms/genetics ; Female ; Gene Frequency/genetics ; Genetic Predisposition to Disease/genetics ; Genotype ; Humans ; Interleukin-6/genetics ; Middle Aged ; Polymorphism, Genetic/genetics ; Risk Factors
    Chemical Substances IL6 protein, human ; Interleukin-6
    Language English
    Publishing date 2016-10
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1375979-6
    ISSN 1532-2807 ; 1219-4956
    ISSN (online) 1532-2807
    ISSN 1219-4956
    DOI 10.1007/s12253-016-0073-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Upper gastrointestinal cancer burden in Hebei Province, China: A population-based study.

    Li, Dao-Juan / Liang, Di / Song, Guo-Hui / Li, Yong-Wei / Wen, Deng-Gui / Jin, Jing / He, Yu-Tong

    World journal of gastroenterology

    2017  Volume 23, Issue 14, Page(s) 2625–2634

    Abstract: Aim: To investigate the incidence and mortality rates of upper gastrointestinal cancer (UGIC) in Hebei Province, China, and to identify high-risk populations to improve UGIC prevention and control.: Methods: Data for UGIC patients were collected from ...

    Abstract Aim: To investigate the incidence and mortality rates of upper gastrointestinal cancer (UGIC) in Hebei Province, China, and to identify high-risk populations to improve UGIC prevention and control.
    Methods: Data for UGIC patients were collected from 21 population-based cancer registries covering 15.25% of the population in Hebei Province. Mortality data were extracted from three national retrospective death surveys (1973-1975, 1990-1992 and 2004-2005). The data were stratified by 5-year age groups, gender and area (high-risk/non-high-risk areas) for analysis. The age-period-cohort and grey system model were used.
    Results: The crude incidence rate of UGIC was 55.47/100000, and the adjusted rate (Segi's population) was 44.90/100000. Males in rural areas had the highest incidence rate (world age-standardized rate = 87.89/100000). The crude mortality rate of UGIC displayed a decreasing trend in Hebei Province from the 1970s to 2013, and the adjusted rate decreased by 43.81% from the 1970s (58.07/100000) to 2013 (32.63/100000). The mortality rate declined more significantly in the high-risk areas (57.26%) than in the non-high-risk areas (55.02%) from the 1970s to 2013. The median age at diagnosis of UGIC was 65.06 years in 2013. There was a notable delay in the median age at death from the 1970s (66.15 years) to 2013 (70.39 years), especially in the high-risk areas. In Cixian, the total trend of the cohort effect declined, and people aged 65-69 years were a population at relatively high risk for UGIC. We predicted that the crude mortality rates of UGIC in Cixian and Shexian would decrease to 98.80 and 133.99 per 100000 in 2018, respectively.
    Conclusion: UGIC was the major cause of cancer death in Hebei Province, and males in rural areas were a high-risk population. We should strengthen early detection and treatment of UGIC in this population.
    Language English
    Publishing date 2017-04-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v23.i14.2625
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Determination and fingerprint analysis of steroidal saponins in roots of Liriope muscari (Decne.) L. H. Bailey by ultra high performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry.

    Li, Yong-Wei / Qi, Jin / Wen-Zhang / Zhou, Shui-Ping / Yan-Wu / Yu, Bo-Yang

    Journal of separation science

    2014  Volume 37, Issue 14, Page(s) 1762–1772

    Abstract: Liriope muscari (Decne.) L. H. Bailey is a well-known traditional Chinese medicine used for treating cough and insomnia. There are few reports on the quality evaluation of this herb partly because the major steroid saponins are not readily identified by ... ...

    Abstract Liriope muscari (Decne.) L. H. Bailey is a well-known traditional Chinese medicine used for treating cough and insomnia. There are few reports on the quality evaluation of this herb partly because the major steroid saponins are not readily identified by UV detectors and are not easily isolated due to the existence of many similar isomers. In this study, a qualitative and quantitative method was developed to analyze the major components in L. muscari (Decne.) L. H. Bailey roots. Sixteen components were deduced and identified primarily by the information obtained from ultra high performance liquid chromatography with ion-trap time-of-flight mass spectrometry. The method demonstrated the desired specificity, linearity, stability, precision, and accuracy for simultaneous determination of 15 constituents (13 steroidal glycosides, 25(R)-ruscogenin, and pentylbenzoate) in 26 samples from different origins. The fingerprint was established, and the evaluation was achieved using similarity analysis and principal component analysis of 15 fingerprint peaks from 26 samples by ultra high performance liquid chromatography. The results from similarity analysis were consistent with those of principal component analysis. All results suggest that the established method could be applied effectively to the determination of multi-ingredients and fingerprint analysis of steroid saponins for quality assessment and control of L. muscari (Decne.) L. H. Bailey.
    MeSH term(s) Chromatography, High Pressure Liquid/methods ; Drugs, Chinese Herbal/chemistry ; Isomerism ; Liriope Plant/chemistry ; Plant Roots/chemistry ; Saponins/chemistry ; Spectrometry, Mass, Electrospray Ionization/methods
    Chemical Substances Drugs, Chinese Herbal ; Saponins
    Language English
    Publishing date 2014-07
    Publishing country Germany
    Document type Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2047990-6
    ISSN 1615-9314 ; 1615-9306
    ISSN (online) 1615-9314
    ISSN 1615-9306
    DOI 10.1002/jssc.201400273
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