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Article ; Online: Absceso cerebral por Nocardia en paciente diagnosticado de proteinosis alveolar.

Godoy Hurtado, A / Liébana Martos, C / Bartschi, P / Al-Ghanem, R / Galicia Bulnes, J M / Saldívar Gómez, J

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2020 Volume 34, Issue 1, Page(s) 61–63

Title translation	Brain abscess caused by Nocardia in patient with alveolar proteinosis.
MeSH term(s)	Brain Abscess/diagnostic imaging ; Brain Abscess/drug therapy ; Humans ; Nocardia ; Nocardia Infections/drug therapy ; Pulmonary Alveolar Proteinosis
Language	Spanish
Publishing date	2020-11-23
Publishing country	Spain
Document type	Letter
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
DOI	10.37201/req/069.2020
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Article ; Online: Prevalencia en varones de la población general de agentes productores de infecciones no ulcerativas del aparato genital, asistidos en atención especializada.

Foronda-García-Hidalgo, C / Liébana-Martos, C / Gutiérrez-Soto, B / Expósito-Ruiz, M / Navarro-Marí, J M / Gutiérrez-Fernández, J

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2019 Volume 32, Issue 6, Page(s) 545–550

Abstract: Objective: Male genital infections are a major problem due to their high frequency and morbidity and their role in cases of male infertility. We studied the presence, in males assisted in specialized care, of non-ulcerative genital tract infections- ... ...

Title translation	Prevalence among males from the general population of agents responsible of not ulcerative genital tract infections, assisted in specialized care.
Abstract	Objective: Male genital infections are a major problem due to their high frequency and morbidity and their role in cases of male infertility. We studied the presence, in males assisted in specialized care, of non-ulcerative genital tract infections-producing agents. Methods: We studied descriptively and retrospective microbiological results of 3,066 samples of male patients, with diagnosis of genital tract infection episode, received between January 1, 2016 and December 31, 2017. Detection of microorganisms in the sample was performed using techniques of artificial culture and PCR (BD-MAX). Results: Positive results were obtained in 451 samples (14.71%). By culture, the most frequent pathogens were Enterobacterales (18.40%), Enterococcus (13.75%), Haemophilus (8.65%), Neisseria gonorrhoeae (8.43%), Ureaplasma (5.10%), and Candida (3.77%). By polymerase chain reaction (PCR), the most frequent were N. gonorrhoeae (28.37%), Chlamydia trachomatis (26.95%), Ureaplasma urealyticum (17.73%), Mycoplasma hominis/Ureaplasma parvum (10.64%), and Mycoplasma genitalium (7.10%). The age was older in patients infected with Enterobacterales, Candida, or Enterococcus and younger in those infected with N. gonorrhoeae. Conclusions: N. gonorrhoeae and C. trachomatis are still more common in male genital infection pathogens, although other culturable microorganisms have an important role. These findings demonstrate the importance of systematically applying both conventional culture and PCR techniques for pathogen detection.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Cross-Sectional Studies ; Humans ; Infant ; Male ; Middle Aged ; Prevalence ; Reproductive Tract Infections/epidemiology ; Reproductive Tract Infections/microbiology ; Retrospective Studies ; Young Adult
Language	Spanish
Publishing date	2019-10-22
Publishing country	Spain
Document type	Journal Article
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
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Article ; Online: Detección de uropatógenos inusuales durante un periodo de 3 años en un hospital regional.

Gómez-Camarasa, C / Liébana-Martos, C / Navarro-Marí, J M / Gutiérrez-Fernández, J

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2015 Volume 28, Issue 2, Page(s) 86–91

Abstract: Urinary tract infections (UTIs) are one of the most frequent both in the community and in hospitals infectious diseases. The etiology of urinary tract infections is well established but may vary depending on various factors such as age, the presence of ... ...

Title translation	Detection of unusual uropathogens during a period of three years in a regional hospital.
Abstract	Urinary tract infections (UTIs) are one of the most frequent both in the community and in hospitals infectious diseases. The etiology of urinary tract infections is well established but may vary depending on various factors such as age, the presence of underlying diseases such as diabetes, instrumental procedures such as urinary catheterization or exposure to antibiotics or previous hospitalizations. UTIs diagnosed cases were retrospectively reviewed for unusual microorganisms over a period of 3 years (2011-2013) in the Microbiology Laboratory of the Hospital Virgen de las Nieves of Granada (Spain), following the standard operating procedure, which we describe four cases caused by Trichosporon asahii, Aerococcus urinae, Pasteurella bettyae and Neisseria sicca. Hence the importance of having in the Clinical Microbiology Laboratory of the tools necessary to detection UTIs and reach a correct identification in all cases.
MeSH term(s)	Adult ; Aged ; Bacteria/isolation & purification ; Colony Count, Microbial ; Cross Infection/microbiology ; Gram-Negative Bacterial Infections/microbiology ; Gram-Negative Bacterial Infections/urine ; Hospitals ; Humans ; Male ; Middle Aged ; Neisseria sicca ; Neisseriaceae Infections/microbiology ; Pasteurella ; Retrospective Studies ; Spain/epidemiology ; Trichosporon ; Trichosporonosis/drug therapy ; Trichosporonosis/microbiology ; Urinary Tract Infections/epidemiology ; Urinary Tract Infections/microbiology
Language	Spanish
Publishing date	2015-04
Publishing country	Spain
Document type	English Abstract ; Journal Article
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
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Article ; Online: MALDI-TOF MS, a useful instrument for differentiating metallo-β-lactamases in Enterobacteriaceae and Pseudomonas spp.

Hoyos-Mallecot, Y / Cabrera-Alvargonzalez, J J / Miranda-Casas, C / Rojo-Martín, M D / Liebana-Martos, C / Navarro-Marí, J M

Letters in applied microbiology

2014 Volume 58, Issue 4, Page(s) 325–329

Abstract: Unlabelled: We have evaluated a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for the identification of carbapenemases and for distinguishing metallo-β-lactamases (MBLs). A total of 49 ... ...

Abstract	Unlabelled: We have evaluated a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for the identification of carbapenemases and for distinguishing metallo-β-lactamases (MBLs). A total of 49 noncarbapenemase-producing and 14 carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa clinical strains, previously characterized by PCR, were included in the study. With MALDI-TOF MS, the presence of carbapenemases was confirmed by the detection of ertapenem hydrolysis (lost of molecular peaks: 476·5 Da, 498·5 Da, 520·5 Da and presence of degradation products) in the mixture of the bacteria with the antibiotic, and classification was achieved by selective inhibition of carbapenemase activity (the ertapenem molecular peak was maintained) with ethylenediaminetetraacetic acid (EDTA). We obtained a good concordance among the results of PCR and MALDI-TOF MS. This method appears to be simple, fast and reliable for distinguishing in few hours different classes of carbapenemases, which can be very useful for epidemiological studies or to establish a specific antimicrobial therapy. Significance and impact of the study: MALDI-TOF mass spectrometry is increasingly present in microbiology laboratories due to its increasing use for bacterial identification. This study describes a method for detection of carbapenemase activity using MALDI-TOF, which is similar to the reference method: the detection of imipenem hydrolysis using UV spectrometry.
MeSH term(s)	Anti-Bacterial Agents/metabolism ; Bacterial Proteins/analysis ; Bacterial Proteins/metabolism ; Enterobacteriaceae/enzymology ; Humans ; Pseudomonas aeruginosa/enzymology ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; beta-Lactamases/analysis ; beta-Lactamases/metabolism ; beta-Lactams/metabolism
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; beta-Lactams ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6) ; ertapenem (G32F6EID2H)
Language	English
Publishing date	2014-04
Publishing country	England
Document type	Evaluation Studies ; Journal Article
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1111/lam.12203
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Article ; Online: Recent clinical relevance of mono-genital colonization/infection by Ureaplasma parvum.

Siles-Guerrero, Víctor / Cardona-Benavides, Inmaculada / Liébana-Martos, Carmen / Vázquez-Alonso, Fernando / Expósito-Ruiz, Manuela / Navarro-Marí, José María / Gutiérrez-Fernández, José

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2020 Volume 39, Issue 10, Page(s) 1899–1905

Abstract: Ureaplasma parvum is the most prevalent genital mycoplasma in women of childbearing age. There is debate around the relevance of its presence in male or female genitals for disease development and as a cofactor. The objective of this study was to ... ...

Abstract	Ureaplasma parvum is the most prevalent genital mycoplasma in women of childbearing age. There is debate around the relevance of its presence in male or female genitals for disease development and as a cofactor. The objective of this study was to determine the prevalence of colonization/infection by U. parvum and its possible relationship with reproductive tract infections. We retrospectively analyzed the presence of U. parvum in patients referred by specialist clinicians for suspicion of genitourinary tract infection. U. parvum was detected in 23.8% of samples, significantly more frequently in females (39.9%) than in males (6%). Among the males, U. parvum was found alone in 68.4% of episodes, with Ct < 30. Among the females, U. parvum was detected in 88.6% of cases, with Ct < 30, including 22 cases with premature rupture of membranes and 6 cases with threat of preterm labor. Co-infection was significantly more frequent in females (62.6%) than in males (31.6%). Given the high prevalence of U. parvum as sole isolate in males and females with genitourinary symptoms, it should be considered in the diagnosis and treatment of genital infections, although its pathogenic role in some diseases has not been fully elucidated.
MeSH term(s)	Adolescent ; Adult ; Cross-Sectional Studies ; Female ; Humans ; Male ; Pregnancy ; Pregnancy Complications, Infectious/epidemiology ; Pregnancy Complications, Infectious/microbiology ; Prevalence ; Reproductive Tract Infections/epidemiology ; Reproductive Tract Infections/microbiology ; Sex Factors ; Spain/epidemiology ; Ureaplasma/genetics ; Ureaplasma/isolation & purification ; Ureaplasma Infections/epidemiology ; Ureaplasma Infections/microbiology ; Young Adult
Language	English
Publishing date	2020-05-21
Publishing country	Germany
Document type	Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-020-03928-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1732: Show issues

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Article ; Online: Streptococcus agalactiae: prevention and vaccine development.

Puertas-Prieto, A / Lara-Oya, A / Liébana Martos, C / Rodríguez-Granger, J / Cobo, F / Sampedro, A / Padilla, A / Gutiérrez-Fernández, J / Manzanares-Galán, S / Cueto-López, M / Rosa-Fraile, M / Navarro-Mari, J M

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2017 Volume 30, Issue 5, Page(s) 312–318

Abstract: Streptococcus agalactiae, group B Streptococcus (SGB), is the most important cause of morbi-mortality among newborn population, and an important pathogen among immunossupressed adult patients. Despite the advances in the treatment and prevention of ... ...

Title translation	Streptococcus agalactiae: prevención y desarrollo de vacunas.
Abstract	Streptococcus agalactiae, group B Streptococcus (SGB), is the most important cause of morbi-mortality among newborn population, and an important pathogen among immunossupressed adult patients. Despite the advances in the treatment and prevention of neonatal infections as a consequence of implementation of national and international recommendations for prevention of infection, there are still some improvements for the final control of the disease. In this sense, the vaccination against SGB could be an effective measure for the prevention of disease in those cases where intrapartum prophylaxis is not useful and in adult patients with risk factors for invasive infection due to SGB. This review summarizes the efforts made until now in order to establish the control of the infection, and brings some information on the current state-of-the art of vaccines against SGB, in which different strategies in their design have been used.
MeSH term(s)	Bacterial Vaccines/therapeutic use ; Female ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases/microbiology ; Infant, Newborn, Diseases/prevention & control ; Pregnancy ; Pregnancy Complications, Infectious ; Streptococcal Infections/epidemiology ; Streptococcal Infections/immunology ; Streptococcal Infections/microbiology ; Streptococcal Infections/prevention & control ; Streptococcus agalactiae/immunology ; Vaccination ; Vaccines, Conjugate
Chemical Substances	Bacterial Vaccines ; Vaccines, Conjugate
Language	English
Publishing date	2017-10
Publishing country	Spain
Document type	Journal Article ; Review
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A multicentre study investigating parameters which influence direct bacterial identification from urine.

Zboromyrska, Yuliya / Bosch, Jordi / Aramburu, Jesus / Cuadros, Juan / García-Riestra, Carlos / Guzmán-Puche, Julia / Liébana Martos, Carmen / Loza, Elena / Muñoz-Algarra, María / Ruiz de Alegría, Carlos / Sánchez-Hellín, Victoria / Vila, Jordi

PloS one

2018 Volume 13, Issue 12, Page(s) e0207822

Abstract: Rapid diagnosis is one of the best ways to improve patient management and prognosis as well as to combat the development of bacterial resistance. The aim of this study was to study parameters that impact the achievement of reliable identification using a ...

Abstract	Rapid diagnosis is one of the best ways to improve patient management and prognosis as well as to combat the development of bacterial resistance. The aim of this study was to study parameters that impact the achievement of reliable identification using a combination of flow cytometry and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF-MS).The study was carried out in nine hospitals in Spain and included 1,050 urine samples with bacterial counts of 5x106 bacteria/ml. MALDI-ToF-MS-based identification was performed according to a previously described protocol. Valid identification by direct MALDI-ToF-MS was obtained in 72.8% of samples, in 80.3% of samples found to be positive by culture, 32.2% of contaminated samples, and 19.7% of negative samples. Among the positives samples with a valid identification the concordance at the species level was 97.2%. The parameters related to success of direct identification were: high bacterial count, the presence of Escherichia coli as a pathogen and rod-bacteria morphology provided by flow cytometry. The parameters related to failure were a high epithelial cell (EC) count, a high white blood cell (WBC) count and urine samples obtained from in-patients. In summary, this multicentre study confirms previously published data on the usefulness and accuracy of direct MALDI-ToF-MS-based identification of bacteria from urine samples. It seems important to evaluate not only the bacterial count, but also other parameters, such as EC and WBC counts, bacterial species and morphology, and the health care setting, to decide whether the sample is suitable for direct identification.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Bacteriological Techniques ; Bacteriuria/diagnosis ; Bacteriuria/microbiology ; Escherichia coli Infections/diagnosis ; Escherichia coli Infections/microbiology ; Female ; Flow Cytometry ; Humans ; Male ; Middle Aged ; Reproducibility of Results ; Spain ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Urinary Tract Infections/diagnosis ; Urinary Tract Infections/microbiology ; Urine/microbiology
Language	English
Publishing date	2018-12-11
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0207822
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: MALDI‐TOF MS, a useful instrument for differentiating metallo‐β‐lactamases in Enterobacteriaceae and Pseudomonas spp

Hoyos‐Mallecot, Y / Cabrera‐Alvargonzalez, J.J / Miranda‐Casas, C / Rojo‐Martín, M.D / Liebana‐Martos, C / Navarro‐Marí, J.M

Letters in applied microbiology. 2014 Apr., v. 58, no. 4

2014

Abstract: We have evaluated a matrix‐assisted laser desorption ionization–time of flight mass spectrometry (MALDI‐TOF MS) method for the identification of carbapenemases and for distinguishing metallo‐β‐lactamases (MBLs). A total of 49 noncarbapenemase‐producing ... ...

Abstract	We have evaluated a matrix‐assisted laser desorption ionization–time of flight mass spectrometry (MALDI‐TOF MS) method for the identification of carbapenemases and for distinguishing metallo‐β‐lactamases (MBLs). A total of 49 noncarbapenemase‐producing and 14 carbapenemase‐producing Enterobacteriaceae and Pseudomonas aeruginosa clinical strains, previously characterized by PCR, were included in the study. With MALDI‐TOF MS, the presence of carbapenemases was confirmed by the detection of ertapenem hydrolysis (lost of molecular peaks: 476·5� Da, 498·5� Da, 520·5� Da and presence of degradation products) in the mixture of the bacteria with the antibiotic, and classification was achieved by selective inhibition of carbapenemase activity (the ertapenem molecular peak was maintained) with ethylenediaminetetraacetic acid (EDTA). We obtained a good concordance among the results of PCR and MALDI‐TOF MS. This method appears to be simple, fast and reliable for distinguishing in few hours different classes of carbapenemases, which can be very useful for epidemiological studies or to establish a specific antimicrobial therapy. SIGNIFICANCE AND IMPACT OF THE STUDY: MALDI‐TOF mass spectrometry is increasingly present in microbiology laboratories due to its increasing use for bacterial identification. This study describes a method for detection of carbapenemase activity using MALDI‐TOF, which is similar to the reference method: the detection of imipenem hydrolysis using UV spectrometry.
Keywords	EDTA (chelating agent) ; Enterobacteriaceae ; Pseudomonas aeruginosa ; bacteria ; desorption ; epidemiological studies ; hydrolysis ; mass spectrometry ; polymerase chain reaction ; therapeutics ; ultraviolet-visible spectroscopy
Language	English
Dates of publication	2014-04
Size	p. 325-329.
Document type	Article
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1111/lam.12203
Database	NAL-Catalogue (AGRICOLA)

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Article: Specimen storage in transport medium and detection of group B streptococci by culture.

Rosa-Fraile, Manuel / Camacho-Muñoz, Enrique / Rodríguez-Granger, Javier / Liébana-Martos, Carmen

Journal of clinical microbiology

2005 Volume 43, Issue 2, Page(s) 928–930

Abstract: Recovery of group B streptococci (GBS) was assessed in 1,204 vaginorectal swabs stored in Amies transport medium at 4 or 21 degrees C for 1 to 4 days either by direct inoculation onto Granada agar (GA) or by culture in blood agar (BA) and GA after a ... ...

Abstract	Recovery of group B streptococci (GBS) was assessed in 1,204 vaginorectal swabs stored in Amies transport medium at 4 or 21 degrees C for 1 to 4 days either by direct inoculation onto Granada agar (GA) or by culture in blood agar (BA) and GA after a selective broth enrichment (SBE) step. Following storage at 4 degrees C, GBS detection in GA was not affected after 72 h by either direct inoculation or SBE; however, GBS were not detected after SBE in the BA subculture in some samples after 48 h of storage and in GA after 96 h. After storage at 21 degrees C, loss of GBS-positive results was significant after 48 h by direct inoculation in GA and after 96 h by SBE and BA subculture; some GBS-positive samples were not detected after 24 h of storage followed by SBE and BA subculture or after 48 h of storage followed by SBE and GA subculture. Storage of swabs in transport medium, even at 4 degrees C, produced after 24 h an underestimation of the intensity of GBS colonization in most specimens. These data indicate that viability of GBS is not fully preserved by storage of vaginorectal swabs in Amies transport medium, mainly if they are not stored under refrigeration.
MeSH term(s)	Bacteriological Techniques ; Culture Media ; Female ; Humans ; Pregnancy ; Pregnancy Complications, Infectious/diagnosis ; Pregnancy Complications, Infectious/microbiology ; Rectum/microbiology ; Specimen Handling/methods ; Streptococcal Infections/diagnosis ; Streptococcal Infections/microbiology ; Streptococcus agalactiae/growth & development ; Streptococcus agalactiae/isolation & purification ; Vagina/microbiology
Chemical Substances	Culture Media
Language	English
Publishing date	2005-02-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.43.2.928-930.2005
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Article: MALDI‐TOF MS, a useful instrument for differentiating metallo‐β‐lactamases in Enterobacteriaceae and Pseudomonas spp

Hoyos‐Mallecot, Y. / Cabrera‐Alvargonzalez, J.J. / Miranda‐Casas, C. / Rojo‐Martín, M.D. / Liebana‐Martos, C. / Navarro‐Marí, J.M.

Letters in applied microbiology

Volume v. 58,, Issue no. 4

Abstract	We have evaluated a matrix‐assisted laser desorption ionization–time of flight mass spectrometry (MALDI‐TOF MS) method for the identification of carbapenemases and for distinguishing metallo‐β‐lactamases (MBLs). A total of 49 noncarbapenemase‐producing and 14 carbapenemase‐producing Enterobacteriaceae and Pseudomonas aeruginosa clinical strains, previously characterized by PCR, were included in the study. With MALDI‐TOF MS, the presence of carbapenemases was confirmed by the detection of ertapenem hydrolysis (lost of molecular peaks: 476·5� Da, 498·5� Da, 520·5� Da and presence of degradation products) in the mixture of the bacteria with the antibiotic, and classification was achieved by selective inhibition of carbapenemase activity (the ertapenem molecular peak was maintained) with ethylenediaminetetraacetic acid (EDTA). We obtained a good concordance among the results of PCR and MALDI‐TOF MS. This method appears to be simple, fast and reliable for distinguishing in few hours different classes of carbapenemases, which can be very useful for epidemiological studies or to establish a specific antimicrobial therapy. SIGNIFICANCE AND IMPACT OF THE STUDY: MALDI‐TOF mass spectrometry is increasingly present in microbiology laboratories due to its increasing use for bacterial identification. This study describes a method for detection of carbapenemase activity using MALDI‐TOF, which is similar to the reference method: the detection of imipenem hydrolysis using UV spectrometry.
Keywords	EDTA (chelating agent) ; therapeutics ; desorption ; mass spectrometry ; bacteria ; hydrolysis ; ultraviolet-visible spectroscopy ; epidemiological studies ; Pseudomonas aeruginosa ; polymerase chain reaction ; Enterobacteriaceae
Language	English
Document type	Article
ISSN	0266-8254
Database	AGRIS - International Information System for the Agricultural Sciences and Technology

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