Article ; Online: Real-time cell analysis
STAR Protocols, Vol 3, Iss 2, Pp 101387- (2022)
A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape
2022
Abstract: Summary: Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the ... ...
| Abstract | Summary: Real-time cell analysis (RTCA) enables high-throughput, quantitative kinetic measurements of cytopathic effect (CPE) in virus-infected cells. Here, we detail a RTCA approach for assessing antibody neutralization. We describe how to evaluate the neutralizing potency of monoclonal antibodies (mAbs) and identify viral escape mutants to antibody neutralization for severe respiratory syndrome coronavirus 2 (SARS-CoV-2).For complete details on the use and execution of this protocol, please refer to Zost et al. (2020) and Suryadevara et al. (2021). |
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| Keywords | Cell-based Assays ; Health Sciences ; High Throughput Screening ; Immunology ; Microbiology ; Antibody ; Science (General) ; Q1-390 |
| Language | English |
| Publishing date | 2022-06-01T00:00:00Z |
| Publisher | Elsevier |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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