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Article ; Online: A long-tailed marine reptile from China provides new insights into the Middle Triassic pachypleurosaur radiation

Guang-Hui Xu / Yi Ren / Li-Jun Zhao / Jun-Ling Liao / Dong-Hao Feng

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 12

Abstract: Abstract Pachypleurosaurs (Pachypleurosauroidea) are a group of small to medium-sized, lizard-like marine reptiles in the Early to Middle Triassic, including Pachypleurosauridae, Keichousauridae and closely related taxa. The group is generally considered ...

Abstract	Abstract Pachypleurosaurs (Pachypleurosauroidea) are a group of small to medium-sized, lizard-like marine reptiles in the Early to Middle Triassic, including Pachypleurosauridae, Keichousauridae and closely related taxa. The group is generally considered as a sauropterygian radiation, but its phylogenetic interrelationships remain highly debated. Here, we present a new pachypleurosaurid, Honghesaurus longicaudalis gen. et sp. nov., from the early Middle Triassic (Anisian, ~ 244 Ma) marine deposits in Luxi, Yunnan, China. The discovery documents the first really long-tailed pachypleurosaur with totally 121 (69 caudal) vertebrae, providing new evidence for the vertebral multiplication and ecological adaption of this group. The long trunk associated with an incredibly long tail could provide Honghesaurus the advantage of maneuverability and energy efficiency for lateral undulatory swimming. Honghesaurus, although possessing a series of autapomorphies, fills the morphological gap between Qianxisaurus from the Ladinian Xingyi Biota and Wumengosaurus from the Anisian Panxian Biota. Phylogenetic studies unite these three pachypleurosaurids as a monophyletic clade above European pachypleurosaurid clades and provide new insights into the interrelationships of this group. Our scenario of pachypleurosaurian phylogeny combined with the stratigraphic data imply that the Tethys Ocean was a west–east corridor for dispersal of pachypleurosaurids from Europe into South China.
Keywords	Medicine ; R ; Science ; Q
Subject code	590
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Simultaneous Removal of Multiple Heavy Metal Ions from River Water Using Ultrafine Mesoporous Magnetite Nanoparticles

Fato Patrice Fato / Da-Wei Li / Li-Jun Zhao / Kaipei Qiu / Yi-Tao Long

ACS Omega, Vol 4, Iss 4, Pp 7543-

2019 Volume 7549

Keywords	Chemistry ; QD1-999
Language	English
Publishing date	2019-04-01T00:00:00Z
Publisher	American Chemical Society
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: UV Irradiation and Salicylic Acid Immersion Enhance the Level of Mulberroside A in Isolated Mulberry BranchesâA Huge Amount of Agro-Waste

Wang, Jiang / Li-Jun Zhao / Yu-Qing Zhang

Waste and biomass valorization. 2017 Dec., v. 8, no. 8

2017

Abstract: Mulberroside A (MA) is an oxyresveratrol diglycoside that has many biological activities and pharmacological functions. To improve MA levels in the bark of isolated mulberry branches (Ramulus mori), a huge amount of agro-waste. We took isolated mulberry ... ...

Abstract	Mulberroside A (MA) is an oxyresveratrol diglycoside that has many biological activities and pharmacological functions. To improve MA levels in the bark of isolated mulberry branches (Ramulus mori), a huge amount of agro-waste. We took isolated mulberry branches of a huge amount of agro-waste as experimental material and chose equivalent sections of branches for physiochemical treatments with three types of ultraviolet light (UVA, UVB and UVC) and salicylic acid. HPLC analysis showed that the most effective treatment on MA content was UVB irradiation in the wavelength range of 290â320Â nm. The experimental results showed that the three types of UV irradiation stimulate the mulberry branch cortex to induce the biosynthesis and secretion of MA. When the isolated branches were soaked in different concentration of salicylic acid for different time, the MA concentration had increased significantly. The stimulatory effect of salicylic acid and UVA on MA synthesis in cortex cells of the isolated branches decreased with branch age; it was greatest in those harvested in June and lowest in those harvested in September. These results indicated that salicylic acid, like UV irradiation, can stimulate mulberry cortex cells and induce the biosynthesis and secretion of MA.
Keywords	bark ; bioactive properties ; biosynthesis ; branches ; cortex ; glycosides ; high performance liquid chromatography ; irradiation ; mulberries ; salicylic acid ; secretion ; ultraviolet radiation ; wavelengths
Language	English
Dates of publication	2017-12
Size	p. 2643-2651.
Publishing place	Springer Netherlands
Document type	Article
ZDB-ID	2541900-6
ISSN	1877-265X ; 1877-2641
ISSN (online)	1877-265X
ISSN	1877-2641
DOI	10.1007/s12649-016-9702-2
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Safinamide prevents lipopolysaccharide (LPS)-induced inflammation in macrophages by suppressing TLR4/NF-κB signaling.

Qian, LuLu / Li, Jun-Zhao / Sun, XueMei / Chen, Jie-Bin / Dai, Ying / Huang, Qiu-Xiang / Jin, Ying-Ji / Duan, Qing-Ning

International immunopharmacology

2021 Volume 96, Page(s) 107712

Abstract: Inflammation is a basal host defense response that eliminates the causes and consequences of infection and tissue injury. Macrophages are the primary immune cells involved in the inflammatory response. When activated by LPS, macrophages release various ... ...

Abstract	Inflammation is a basal host defense response that eliminates the causes and consequences of infection and tissue injury. Macrophages are the primary immune cells involved in the inflammatory response. When activated by LPS, macrophages release various pro-inflammatory cytokines, chemokines, inflammatory mediators, and MMPs. However, unbridled inflammation causes further damage to tissues. Safinamide is a selective and reversible monoamine oxidase B (MAOB) inhibitor that has been used for the treatment of Parkinson's disease. In this study, we aimed to investigate whether safinamide has effects on LPS-treated macrophages. Our results show that safinamide inhibited the expression of pro-inflammatory cytokines such as IL-1α, TNF-α, and IL-6. Furthermore, safinamide suppressed the production of CXCL1 and CCL2, thereby preventing leukocyte migration. In addition, safinamide reduced iNOS-derived NO, COX-2-derived PGE
MeSH term(s)	Alanine/analogs & derivatives ; Alanine/pharmacology ; Anti-Inflammatory Agents/pharmacology ; Benzylamines/pharmacology ; Cyclooxygenase 2/genetics ; Cyclooxygenase 2/metabolism ; Cytokines/metabolism ; Dinoprostone/metabolism ; Humans ; Inflammation/chemically induced ; Inflammation/prevention & control ; Inflammation Mediators/metabolism ; Lipopolysaccharides/toxicity ; Macrophages/drug effects ; Matrix Metalloproteinase 2/genetics ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/genetics ; Matrix Metalloproteinase 9/metabolism ; NF-kappa B/antagonists & inhibitors ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/genetics ; Nitric Oxide Synthase Type II/metabolism ; Signal Transduction/drug effects ; Toll-Like Receptor 4/antagonists & inhibitors ; Toll-Like Receptor 4/genetics ; Toll-Like Receptor 4/metabolism ; U937 Cells
Chemical Substances	Anti-Inflammatory Agents ; Benzylamines ; Cytokines ; Inflammation Mediators ; Lipopolysaccharides ; NF-kappa B ; TLR4 protein, human ; Toll-Like Receptor 4 ; Nitric Oxide (31C4KY9ESH) ; safinamide (90ENL74SIG) ; NOS2 protein, human (EC 1.14.13.39) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Cyclooxygenase 2 (EC 1.14.99.1) ; MMP2 protein, human (EC 3.4.24.24) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; MMP9 protein, human (EC 3.4.24.35) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Dinoprostone (K7Q1JQR04M) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2021-05-31
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2021.107712
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Article ; Online: Supervised spatial classification of multispectral LiDAR data in urban areas.

Lian-Zhi Huo / Carlos Alberto Silva / Carine Klauberg / Midhun Mohan / Li-Jun Zhao / Ping Tang / Andrew Thomas Hudak

PLoS ONE, Vol 13, Iss 10, p e

2018 Volume 0206185

Abstract: Multispectral LiDAR (light detection and ranging) data have been initially used for land cover classification. However, there are still high classification uncertainties, especially in urban areas, where objects are often mixed and confounded. This study ...

Abstract	Multispectral LiDAR (light detection and ranging) data have been initially used for land cover classification. However, there are still high classification uncertainties, especially in urban areas, where objects are often mixed and confounded. This study investigated the efficiency of combining advanced statistical methods and LiDAR metrics derived from multispectral LiDAR data for improving land cover classification accuracy in urban areas. The study area is located in Oshawa, Ontario, Canada, on the Lake Ontario shoreline. Multispectral Optech Titan LiDAR data over the study area were acquired on 3 September 2014 in a single strip of 3 km2. Using the channels at 1,550 nm (C1), 1,064 nm (C2) and 532 nm (C3), LiDAR intensity data, normalized digital surface model (nDSM), pseudo normalized difference vegetation index (PseudoNDVI), morphological profiles (MP), and a novel hierarchical morphological profiles (HMP) were derived and used as features for the classification. A support vector machine classifier with a radial basis function (RBF) kernel was applied in the classification stage, where the optimal parameters for the classifier were selected by a grid search procedure. The combination of intensity, pseudoNDVI, nDSM and HMP resulted in the best land cover classification, with an overall accuracy of 93.28%.
Keywords	Medicine ; R ; Science ; Q
Subject code	333
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Electrocatalytic Oxidation of Tris(2-carboxyethyl)phosphine at Pyrroloquinoline Quinone Modified Carbon Nanotube through Single Nanoparticle Collision

Patrice, Fato Tano / Da-Wei Li / Essy Kouadio Fodjo / Kaipei Qiu / Li-Jun Zhao / Yi-Tao Long

Analytical chemistry. 2018 Apr. 27, v. 90, no. 10

2018

Abstract: Inspired by the addition-elimination catalytic mechanism of natural pyrroloquinoline quinone (PQQ) containing proteins, PQQ-modified hybrid nanomaterials have been increasingly developed recently as biomimetic heterogeneous electrocatalysts. However, up ... ...

Abstract	Inspired by the addition-elimination catalytic mechanism of natural pyrroloquinoline quinone (PQQ) containing proteins, PQQ-modified hybrid nanomaterials have been increasingly developed recently as biomimetic heterogeneous electrocatalysts. However, up until now, no existing electrochemical approach was able to assess the intrinsic catalytic activity of PQQ sites, impeding the design of efficient PQQ-based electrocatalysts. Herein, in this work, we introduced a new method to calculate the turnover frequency (TOF) of any individual PQQ functional group for electrocatalytic oxidation of tris(2-carboxyethyl)phosphine (TCEP), through the study of single PQQ-decorated carbon nanotube (CNT) collisions at a carbon fiber ultramicroelectrode by chronoamperometry. The core advantage of this approach is being able to resolve the number of PQQ catalytic sites grafted on each individual CNT, so that the charge of any CNT collision event can be accurately translated into the intrinsic activity of the respective PQQ functional groups. The resulting collision-induced current responses clearly showed that the functionalization of CNTs with PQQ could indeed enhance its catalytic performance by 3 times, reaching a TOF value of 133 s–1 at 1.0 V vs Ag/AgCl. Such a single CNT collision technique, which is proposed for the first time in this work, can open up a new avenue for studying the intrinsic (electro)catalytic performance at a molecular level.
Keywords	active sites ; biomimetics ; carbon fibers ; carbon nanotubes ; catalytic activity ; electrochemistry ; moieties ; nanoparticles ; oxidation ; proteins ; quinones ; silver ; silver chloride
Language	English
Dates of publication	2018-0427
Size	p. 6059-6063.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.7b05405
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Study of the Characterization of Side Population Cells in Endometrial Cancer Cell Lines

Bing-jie Liu / Qi-ying Xu / Wei-dong Yu / Na Li / Tian Yao / Li-jun Zhao / Jian-liu Wang / Li-hui Wei / Xiao-ping Li

Frontiers in Medicine, Vol

Chemoresistance, Progestin Resistance, and Radioresistance

2020 Volume 7

Abstract: Introduction: Radiotherapy, combined regimens as platinum-paclitaxel chemotherapy and/or endocrine therapy is an important adjuvant treatment after surgery for endometrial cancer (EC). While, the resistance to them remain unclear. In our study, to ... ...

Abstract	Introduction: Radiotherapy, combined regimens as platinum-paclitaxel chemotherapy and/or endocrine therapy is an important adjuvant treatment after surgery for endometrial cancer (EC). While, the resistance to them remain unclear. In our study, to separate the characteristics of side population (SP) cells from EC cell lines, study the mechanism of Taxol-resistance, progestin resistance and radioresistanc, and provide the basic for EC.Methods: SP cells from EC cell lines HEC-1A, Ishikawa and RL95-2 were separated by Hoechst 33342 staining and flow cytometry analysis. The expression of breast cancer resistance protein (BCRP) in SP cells and non-SP cells from HEC-1A was examined by immunocytochemistry, and the radiation-resistant and Taxol-resistant characteristics of SP cells and non-SP cells were compared by MTS. Ishikawa, Ishikawa-SP, and Ishikawa-non-SP cells incubated with MPA were selected for cell apoptosis assays by using flow cytometry. The expression of caspase-3 was examined by immunocytochemistry, and autophagy was detected by MDC staining.Results: Small proportions of SP cells, namely, 1.44 ± 0.93%, 2.86 ± 3.09%, and 2.87 ± 1.29%, were detected in HEC-1A, Ishikawa and RL95-2, respectively. There was a stronger clone formation efficiency for the SP cells than for non-SP cells in HEC-1A [(6.02 ± 1.17) vs. (0.53±0.20)%, P = 0.001], and there was a significant difference in the rate of tumourigenicity between the SP cells and non-SP cells in HEC-1A (87.5 vs. 12.5%). There were higher levels of BCRP expression (P = 0.001) and resistance to Taxol and radiation (P < 0.05) in the SP cells than in non-SP cells. After MPA treatment, the apoptosis rates were significantly different among the Ishikawa, Ishikawa-SP and Ishikawa-non-SP groups [(4.64 ± 0.18)%, (4.01 ± 0.43)%, and (9.3 ± 0.67)%; (P = 0.05)], and the expression of Caspase-3 in the Ishikawa group was higher than that in Ishikawa-SP group. The autophagic activity of the Ishikawa-SP cells was the strongest, while the autophagic activity of ...
Keywords	endometrial cancer ; side population cells ; chemoresistance ; radioresistance ; breast cancer resistance protein (BCRP) ; Medicine (General) ; R5-920
Subject code	610
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Characterization of extracellular polymeric substances and microbial diversity in anaerobic co-digestion reactor treated sewage sludge with fat, oil, grease

Yang, Zhao-Hui / Li-Jun Zhao / Min Li / Rui Xu / Ting Chen / Yue Zheng

Bioresource technology. 2016 July, v. 212

2016

Abstract: Performance of co-digesters, treated of sewage sludge (SS) with fat, oil and grease (FOG), were conducted semi-continuously in two mesophilic reactors over 180days. Compared with SS mono-digestion, biogas production and TS removal efficiency of co- ... ...

Abstract	Performance of co-digesters, treated of sewage sludge (SS) with fat, oil and grease (FOG), were conducted semi-continuously in two mesophilic reactors over 180days. Compared with SS mono-digestion, biogas production and TS removal efficiency of co-digestion were significantly enhanced up to 35% and 26% by adding upper limit FOG (60% on VS). Enhancement in co-digestion performance was also stimulated by the release of extracellular polymeric substances (EPS), which was increased 40% in both loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS) than that of mono-digester. Based on high-throughput sequencing (HTS), analysis of microbial 16S rRNA gene comprehensively revealed the dynamic change of microbial community. Results showed that both bacterial and archaeal undergone an apparent succession with FOG addition, and large amount of consortium like Methanosaeta and N09 were involved in the process. Redundancy analysis showed the acetoclastic genera Methanosaeta distinctly related with biogas production and EPS degradation.
Keywords	anaerobic digestion ; biogas ; genes ; high-throughput nucleotide sequencing ; Methanosaeta ; microbial communities ; oils ; ribosomal RNA ; sewage sludge ; sewage treatment
Language	English
Dates of publication	2016-07
Size	p. 164-173.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2016.04.046
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Intra-tumor heterogeneity for endometrial cancer and its clinical significance

Fu-Fen Yin / Li-Jun Zhao / Xiao-Yu Ji / Ning Duan / Yan-Kui Wang / Jing-Yi Zhou / Li-Hui Wei / Xiang-Jun He / Jian-Liu Wang / Xiao-Ping Li / Li-Shao Guo

Chinese Medical Journal, Vol 132, Iss 13, Pp 1550-

2019 Volume 1562

Abstract: Abstract. Background:. Management of tumors has become more complex owing to tumor heterogeneity. Fewer studies have been performed on intra-tumor heterogeneity of endometrial cancer (EC) until now. Therefore, it is of great clinical value to explore the ...

Abstract	Abstract. Background:. Management of tumors has become more complex owing to tumor heterogeneity. Fewer studies have been performed on intra-tumor heterogeneity of endometrial cancer (EC) until now. Therefore, it is of great clinical value to explore the intra-tumor heterogeneity of EC based on clinical features and gene expression profiles. Methods:. A total of 1688 patients with EC were screened and 114 patients were finally selected, including specimens from 84 patients with primary EC without relapse (PE) and the paired metastases (P-M) specimens, as well as specimens from 30 patients with primary EC with relapse (RPE) and the paired relapsed EC (P-RE) specimens. Microarray and RNA-seq were used to detect gene expression of EC samples. Clinicopathological characteristics and molecular data were compared between PE and P-M groups and between RPE and P-RE groups to explore the intra-tumor heterogeneity of EC. Results:. The clinical intra-tumor spatial heterogeneity of pathological type, grade, ER status, and PR status between PE and P-M were 17.9%, 13.1%, 28.6%, and 28.6%, respectively. The clinical intra-tumor spatiotemporal heterogeneity of pathological type, grade, ER status, and PR status between RPE and P-RE were 16.7%, 33.3%, 25.0%, and 37.5%, respectively. Cluster analysis sorts EC samples based on progression type of lesion and their pathological type. There were differentially expressed genes between PE and P-M and between RPE and P-RE, of which gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis were mainly enriched in cell proliferation, the p53 signaling pathway, etc. Conclusions:. Clinical and molecular data showed that there was spatiotemporal heterogeneity in intra-tumor of EC, which may add to the complexity of diagnosis and therapeutics for EC. Considering the intra-tumor heterogeneity, sequential chemotherapy and precision medicine may be a more suitable treatment plan for EC.
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2019-07-01T00:00:00Z
Publisher	Wolters Kluwer
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Identification of new fusion genes and their clinical significance in endometrial cancer

Tian Yao / Jin-Jin Liu / Li-Jun Zhao / Jing-Yi Zhou / Jia-Qi Wang / Yue Wang / Zhi-Qi Wang / Li-Hui Wei / Jian-Liu Wang / Xiao-Ping Li / Yi Cui

Chinese Medical Journal, Vol 132, Iss 11, Pp 1314-

2019 Volume 1321

Abstract: Abstract. Background:. Fusion genes may play an important role in tumorigenesis, prognosis, and drug resistance; however, studies on fusion genes in endometrial cancer (EC) are rare. This study aimed to identify new fusion genes and to explore their ... ...

Abstract	Abstract. Background:. Fusion genes may play an important role in tumorigenesis, prognosis, and drug resistance; however, studies on fusion genes in endometrial cancer (EC) are rare. This study aimed to identify new fusion genes and to explore their clinical significance in EC. Methods:. A total of 28 patients diagnosed with EC were enrolled in this study. RNA sequencing was used to obtain entire genomes and transcriptomes. STAR-comparison and STAR-fusion prediction were applied to predict the fusion genes. Chi-square tests and Student t tests were used to verify the clinical significance with SPSS 13.0 software. Results:. New fusion genes were found, and the number of fusion genes varied from 3 to 110 among all patients with EC. The type of fusion genes varied and included messenger RNA (mRNA)-mRNA, long non-coding RNA (lncRNA)-lncRNA, and lncRNA-mRNA. There were six fusion genes with high fusion rates, namely, RP11–123O10.4–GRIP1, RP11–444D3.1–SOX5, RP11–680G10.1–GSE1, NRIP1–AF127936.7, RP11–96H19.1–RP11–446N19.1, and DPH7–PTP4A3. Further studies showed that these fusion genes are related to stage, grade, and recurrence, in which NRIP1–AF127936.7 and DPH7–PTP4A3 were found only in stage III patients with EC. DPH7–PTP4A3 was found in grades 2 and 3, and recurrent patients with EC. Conclusion:. Fusion genes play an essential role in EC. Six genes that are overexpressed with high fusion rates are identified. NRIP1–AF127936.7 and DPH7–PTP4A3 might be related to stage, and DPH7–PTP4A3 be related to grade and recurrence.
Keywords	Medicine ; R
Subject code	610 ; 616
Language	English
Publishing date	2019-06-01T00:00:00Z
Publisher	Wolters Kluwer
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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