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  1. Article ; Online: THE INTEGRATION OF LOCAL ADMINISTRATION IN THE ROMANIAN PUBLIC AND ECONOMIC SPACE

    Gheorghe CRIŞAN / Lia Ioana CRIŞAN

    USV Annals of Economics and Public Administration, Vol 9, Iss Special Number, Pp 29-

    2009  Volume 29

    Abstract: The convergence of the Romanian government to the European public space requires a permanent effort ofadaptation, which should make use of constructive constituents specific to our country and to apply them thecharacteristics of modern and efficient ... ...

    Abstract The convergence of the Romanian government to the European public space requires a permanent effort ofadaptation, which should make use of constructive constituents specific to our country and to apply them thecharacteristics of modern and efficient administrations.Continuous changes of expectations and the interests of citizens requires, however, that the publicadministration (and especially the local one) to continuously reform, which increases the difficulty of this process,which requires a flexible upper of public management.It is important, however, that - despite these difficulties - the process to be done in a legal and organizationalframework, on solid and firm coordinates, based on modern European principles, without affecting a specific area ofcity administration.This paper tries to x-ray this state of facts and proposes some interesting approaches related to themechanisms of integration of local public administration- LPA in this space.This way, analyzing the organization and functioning of the public sector, the paper stresses the changesoccurring in this area following the more pronounced implementation in the administration of the 3 E principle(economy, efficiency and effectiveness), on the background of an intense manifestation of local autonomy and financialdecentralization.The authors note and highlight the phenomena o "slipping" of the public sector towards the private sector,due to the emergence of competition and the need for efficiency and effectiveness in the use of public financialresources, which resizes the mode of action and responsibility for public management, decreasing this way the politicalconnotations
    Keywords local administration ; convergence ; reform ; integration ; competition ; Business ; HF5001-6182 ; Commerce ; HF1-6182 ; Social Sciences ; H ; DOAJ:Business and Management ; DOAJ:Business and Economics
    Subject code 300
    Language Romanian
    Publishing date 2009-12-01T00:00:00Z
    Publisher Universitatii Stefan cel Mare din Suceava
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: PARTICULARITIES OF INFORMATION AND COMMUNICATION IN MANAGING EUROPEAN FUNDS

    GH. CRIŞAN / LIA-IOANA CRIŞAN

    Lucrări Ştiinţifice : Management Agricol, Vol XI, Iss 2, Pp 251-

    2009  Volume 256

    Abstract: European public funds management requires the use of a set of documents and procedures, rigorously followed and severely sanctioned for infringement of their appearance or finding irregularities. Responsible for the efficient use of financial support for ...

    Abstract European public funds management requires the use of a set of documents and procedures, rigorously followed and severely sanctioned for infringement of their appearance or finding irregularities. Responsible for the efficient use of financial support for Member States, the European Union also requires, in the information and communication area, a number of specific requirements, which highlights numerous performance features compared to similar processes in our country and abroad. Information and publicity accompany practically all stages of project cycle management, thus ensuring the support of the transparency and visibility of the process of accessing and managing European funds.
    Keywords information ; communication ; transparency ; visibility ; funds ; Agriculture (General) ; S1-972 ; Agriculture ; S ; DOAJ:Agriculture (General) ; DOAJ:Agriculture and Food Sciences
    Subject code 306
    Language English
    Publishing date 2009-05-01T00:00:00Z
    Publisher Universitatea de Ştiinţe Agricole şi Medicină Veterinară a Banatului Timişoara
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Engineered probiotics for local tumor delivery of checkpoint blockade nanobodies.

    Gurbatri, Candice R / Lia, Ioana / Vincent, Rosa / Coker, Courtney / Castro, Samuel / Treuting, Piper M / Hinchliffe, Taylor E / Arpaia, Nicholas / Danino, Tal

    Science translational medicine

    2020  Volume 12, Issue 530

    Abstract: Checkpoint inhibitors have revolutionized cancer therapy but only work in a subset of patients and can lead to a multitude of toxicities, suggesting the need for more targeted delivery systems. Because of their preferential colonization of tumors, ... ...

    Abstract Checkpoint inhibitors have revolutionized cancer therapy but only work in a subset of patients and can lead to a multitude of toxicities, suggesting the need for more targeted delivery systems. Because of their preferential colonization of tumors, microbes are a natural platform for the local delivery of cancer therapeutics. Here, we engineer a probiotic bacteria system for the controlled production and intratumoral release of nanobodies targeting programmed cell death-ligand 1 (PD-L1) and cytotoxic T lymphocyte-associated protein-4 (CTLA-4) using a stabilized lysing release mechanism. We used computational modeling coupled with experimental validation of lysis circuit dynamics to determine the optimal genetic circuit parameters for maximal therapeutic efficacy. A single injection of this engineered system demonstrated an enhanced therapeutic response compared to analogous clinically relevant antibodies, resulting in tumor regression in syngeneic mouse models. Supporting the potentiation of a systemic immune response, we observed a relative increase in activated T cells, an abscopal effect, and corresponding increases in systemic T cell memory populations in mice treated with probiotically delivered checkpoint inhibitors. Last, we leveraged the modularity of our platform to achieve enhanced therapeutic efficacy in a poorly immunogenic syngeneic mouse model through effective combinations with a probiotically produced cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF). Together, these results demonstrate that our engineered probiotic system bridges synthetic biology and immunology to improve upon checkpoint blockade delivery.
    MeSH term(s) Animals ; CTLA-4 Antigen ; Disease Models, Animal ; Humans ; Immunotherapy ; Mice ; Neoplasms/therapy ; Probiotics ; Single-Domain Antibodies ; T-Lymphocytes
    Chemical Substances CTLA-4 Antigen ; Single-Domain Antibodies
    Language English
    Publishing date 2020-02-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2518854-9
    ISSN 1946-6242 ; 1946-6234
    ISSN (online) 1946-6242
    ISSN 1946-6234
    DOI 10.1126/scitranslmed.aax0876
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Engineering tumor-colonizing E. coli Nissle 1917 for detection and treatment of colorectal neoplasia.

    Gurbatri, Candice R / Radford, Georgette A / Vrbanac, Laura / Im, Jongwon / Thomas, Elaine M / Coker, Courtney / Taylor, Samuel R / Jang, YoungUk / Sivan, Ayelet / Rhee, Kyu / Saleh, Anas A / Chien, Tiffany / Zandkarimi, Fereshteh / Lia, Ioana / Lannagan, Tamsin R M / Wang, Tongtong / Wright, Josephine A / Kobayashi, Hiroki / Ng, Jia Q /
    Lawrence, Matt / Sammour, Tarik / Thomas, Michelle / Lewis, Mark / Papanicolas, Lito / Perry, Joanne / Fitzsimmons, Tracy / Kaazan, Patricia / Lim, Amanda / Stavropoulos, Alexandra M / Gouskos, Dion A / Marker, Julie / Ostroff, Cheri / Rogers, Geraint / Arpaia, Nicholas / Worthley, Daniel L / Woods, Susan L / Danino, Tal

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 646

    Abstract: Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment. Here, first, we demonstrate selective colonization of colorectal adenomas after oral delivery of probiotic E. coli Nissle 1917 (EcN) ...

    Abstract Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment. Here, first, we demonstrate selective colonization of colorectal adenomas after oral delivery of probiotic E. coli Nissle 1917 (EcN) to a genetically-engineered murine model of CRC predisposition and orthotopic models of CRC. We next undertake an interventional, double-blind, dual-centre, prospective clinical trial, in which CRC patients take either placebo or EcN for two weeks prior to resection of neoplastic and adjacent normal colorectal tissue (ACTRN12619000210178). We detect enrichment of EcN in tumor samples over normal tissue from probiotic-treated patients (primary outcome of the trial). Next, we develop early CRC intervention strategies. To detect lesions, we engineer EcN to produce a small molecule, salicylate. Oral delivery of this strain results in increased levels of salicylate in the urine of adenoma-bearing mice, in comparison to healthy controls. To assess therapeutic potential, we engineer EcN to locally release a cytokine, GM-CSF, and blocking nanobodies against PD-L1 and CTLA-4 at the neoplastic site, and demonstrate that oral delivery of this strain reduces adenoma burden by ~50%. Together, these results support the use of EcN as an orally-deliverable platform to detect disease and treat CRC through the production of screening and therapeutic molecules.
    MeSH term(s) Animals ; Humans ; Mice ; Adenoma/diagnosis ; Adenoma/therapy ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/therapy ; Escherichia coli/genetics ; Prospective Studies ; Salicylates ; Double-Blind Method
    Chemical Substances Salicylates
    Language English
    Publishing date 2024-01-20
    Publishing country England
    Document type Clinical Trial ; Journal Article ; Multicenter Study
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-44776-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Colorectal cancer detection and treatment with engineered probiotics.

    Gurbatri, Candice R / Radford, Georgette / Vrbanac, Laura / Coker, Courtney / Im, Jong-Won / Taylor, Samuel R / Jang, YoungUk / Sivan, Ayelet / Rhee, Kyu / Saleh, Anas A / Chien, Tiffany / Zandkarimi, Fereshteh / Lia, Ioana / Lannagan, Tamsin Rm / Wang, Tongtong / Wright, Josephine A / Thomas, Elaine / Kobayashi, Hiroki / Ng, Jia Q /
    Lawrence, Matt / Sammour, Tarik / Thomas, Michelle / Lewis, Mark / Papanicolas, Lito / Perry, Joanne / Fitzsimmons, Tracy / Kaazan, Patricia / Lim, Amanda / Marker, Julie / Ostroff, Cheri / Rogers, Geraint / Arpaia, Nicholas / Worthley, Daniel L / Woods, Susan L / Danino, Tal

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment strategies. Here, we demonstrate the phenomenon of selective, long-term colonization of colorectal adenomas after oral delivery of ... ...

    Abstract Bioengineered probiotics enable new opportunities to improve colorectal cancer (CRC) screening, prevention and treatment strategies. Here, we demonstrate the phenomenon of selective, long-term colonization of colorectal adenomas after oral delivery of probiotic
    Language English
    Publishing date 2023-04-05
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.04.03.535370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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